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Genetic Transformation
Genetic Transformation

... Plasmids exist in bacteria, yeast, organelles Single or multiple plasmid copies per cell Easy to isolate and manipulate Used as vector for transforming bacteria with foreign DNA Foreign DNA is inserted after cutting with restriction enzymes Plasmids contain certain genes which offer a competitive ad ...
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... What is the phenotype of the offspring in box with an “X”? ___________Brown eyes____________ What is the genotype of the offspring in the box with an “X”? __________BB___________ What is the genotypic ratio of the offspring of the above cross? _________1:2:1_______________ ...
Lecture 11 Analysis of Gene Sequences Anatomy of a bacterial
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... Now let’s consider how to obtain DNA segments that are suitable for sequencing. At first, DNA sequences were obtained from cloned DNA segments (we will discuss some methods to clone new genes in a subsequent lecture). Presently the entire DNA sequence for E. coli, as well as a variety of other bacte ...
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Studying the Embryo Lethality of AT5G03220
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Cell-free fetal DNA

Cell-free fetal DNA (cffDNA) is fetal DNA circulating freely in the maternal blood stream. It can be sampled by venipuncture on the mother. Analysis of cffDNA provides a method of non-invasive prenatal diagnosis.cffDNA originates from the trophoblasts making up the placenta. It is estimated that 2-6% of the DNA in the maternal blood is fetal in origin. The fetal DNA is fragmented and makes its way into the maternal bloodstream via shedding of the placental microparticles into the maternal bloodstream (figure 1). Studies have shown that cffDNA can first be observed as early as 7 weeks gestation, and the amount of cffDNA increases as the pregnancy progresses. cffDNA diminishes quickly after the birth of the baby, so that it is no longer detectable in the maternal blood approximately 2 hours after birth. cffDNA is significantly smaller than the maternal DNA in the bloodstream, with fragments approximately 200bp in size. Many protocols to extract the fetal DNA from the maternal plasma use its size to distinguish it from the maternal DNA.Studies have looked at, and some even optimized, protocols for testing non-compatible RhD factors, sex determination for X-linked genetic disorders and testing for single gene disorders. Current studies are now looking at determining aneuploidies in the developing fetus. These protocols can be done earlier than the current prenatal testing methods, and have no risk of spontaneous abortion, unlike current prenatal testing methods. Non-invasive prenatal diagnosis (NIPD) has been implemented in the UK and parts of the US; it has clear benefits above the standard tests of chorionic villi sample (CVS) and amniocentesis which have procedure-related miscarriage risks of about 1 in 100 pregnancies and 1 in 200 pregnancies, respectively.As a method of prenatal diagnosis, cell-free fetal DNA techniques share the same ethical and practical issues, such as the possibility of prenatal sex discernment and sex selection.
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