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A mutation in the Zn-finger of the GAL4
A mutation in the Zn-finger of the GAL4

... derepression of the gene in the absence of a fermentable carbon source (8). Induction of the gene in response to oxygen is controlled by different transcription factors, HAP1 and RC2 which bind to the adjacent UAS 1 element (9). Here we present an example in which a single transcription factor LAC9 ...
Tools for functional annotation
Tools for functional annotation

... – Many tools available for modeling using the GO – Can use computational or manual curation to add annotations ...
High-Efficiency DNA Separation by Capillary Electrophoresis in a
High-Efficiency DNA Separation by Capillary Electrophoresis in a

... polymer concentration and chemical components. For sieving matrixes prepared from cellulose and its derivatives, concentrated solutions are generally required in order to get satisfactory separation of smaller DNA fragments (<1000 bp) because of the smaller mesh size of these solutions. The viscosit ...
Document
Document

... 1. Inoculate broth (usually GMM) with AF spores by gently scraping colony until tip is green and dunking it in the media. Twice should be enough. Grow overnight. Usually 10 ml of broth in a 50 ml centrifuge is adequate but 50 ml in a 250 ml flask may be necessary for large scale extractions. 2. Ther ...
Lab Investigation: Examining a Single Gene
Lab Investigation: Examining a Single Gene

... You will load both your PCR reactions and standard DNA markers sample into the gel. A standard DNA marker has a bunch of different sized pieces of DNA so you can compare it to the DNA from your PCR reaction to figure out what size piece it is. Two or three groups can share a gel, but only one molecu ...
here - Science in School
here - Science in School

... 9. Can you think of reasons why, if you are studying more distantly related organisms, it is better to compare amino acid sequences than DNA sequences? Looking at the amino acid sequences gives a functional meaning to the mutations, which DNA sequences do not provide. For example, silent mutations i ...
Transcription - Faculty Web Pages
Transcription - Faculty Web Pages

here
here

... with TALEs, a newly discovered class of DNA binding proteins. TALEs can ...
Approaches to Repeat Finding
Approaches to Repeat Finding

... Cornell Genomics Forum, 2005-03-18 ...
a-bugno.vp:CorelVentura 7.0
a-bugno.vp:CorelVentura 7.0

... well as mutations associated with genetic diseases. The domestic dog (Canis familiaris) is a species in which over 400 genetically-based diseases have been described, many similar to human diseases (COTMAN & HEAD 2008). For this reason, the dog has become a model species to study the molecular basis ...
Goldmine: Integrating information to place sets of genomic ranges
Goldmine: Integrating information to place sets of genomic ranges

... genomic ranges from any source can be loaded, for example, from BED files. Here we show an example of loading data from the Roadmap Epigenomics Project. In this example, we are interested in how DMRs may overlap and interact with known regulatory regions. The Roadmap Epigenomics Project produced uni ...
Restriction Digests of DNA, Part Two
Restriction Digests of DNA, Part Two

... Last week one of Ms. Levine’s students committed a crime in her class! With the use of DNA you are going to help figure out “who done it”….Ashley Siqueiros brought a brand new lipstick to class. She unsealed it after sitting down in class, but did not open it. There were three other girls sitting at ...
Structure and function of nucleases in DNA repair: shape
Structure and function of nucleases in DNA repair: shape

... al., 1999). This classification has also revealed the relationships between nucleases and identified several new nuclease families. In addition to the classifications of primary sequences, 3D structural data have been rapidly accumulating with respect to the proteins involved in DNA repair, includin ...
Dr. Alison Van Eenennaam
Dr. Alison Van Eenennaam

... Animal Biotechnology and Genomics Education ...
genetic variation in isoniazid metabolism genes
genetic variation in isoniazid metabolism genes

... generally includes the administration of isoniazid (INH), a drug known to cause hepatotoxicity as a potentially serious side effect. INH-induced hepatotoxicity derives from toxic metabolites produced during INH breakdown. Genetic polymorphisms in Nacetyltransferase 2 (NAT2), a core enzyme in INH met ...
Table S10
Table S10

... prototypical VirB system, again, is renowned for introducing T-DNA of the Ti plasmid of Agrobacterium tumefaciens into plant hosts [8]. T4SS homologs are ubiquitous in Alcaligenaceae, with the relevant operons being chromosomal in Te and Bb but borne on the plasmids pWTk445 and pA81 in Tk and A8 res ...
12.3 How Is Biotechnology Used In Forensic Science?
12.3 How Is Biotechnology Used In Forensic Science?

... bred together to produce homozygous transgenic organisms. • Companies have developed salmon and trout with modified or added growth-hormone genes, which make the fish grow much faster than wild fish. • Because medicines are generally more valuable than meat, many researchers are developing animals t ...
Chapter 7: Genetics Lesson 7.3: Human Genetics and Biotechnology
Chapter 7: Genetics Lesson 7.3: Human Genetics and Biotechnology

... http://www.hhmi.org/biointeractive/gender/Y_evolution.html ...
HCS 825 Advanced Plant Breeding
HCS 825 Advanced Plant Breeding

... • Genetic improvement through crossing plants with desired traits and selecting progeny with improved performance and/or improved combinations of traits. • “Accelerated” and “targeted evolution”. • Application of genetics principles to crop improvement. • Systematic procedures used to improve trait ...
Comprehensive genetic approaches to cleft lip/palate
Comprehensive genetic approaches to cleft lip/palate

... • Our laboratory is funded in part by support from the NIDCR • There will be no discussion or endorsement of any products in this presentation ...
Bio2Unit3-7.14.15 - Grainger County Schools
Bio2Unit3-7.14.15 - Grainger County Schools

Divergent evolution and molecular adaptation in
Divergent evolution and molecular adaptation in

... evidence for the action of positive selection in the evolution of these genes, both in insects and in vertebrates [e.g. [1-7]. In addition, olfactory-specific gene families might contribute to the host-specificity shifts occurring in the diversification of super-specialist Drosophila species [8,9]. ...
Chapter 9
Chapter 9

... Figure 9.14 Gene silencing could provide treatments for a wide range of diseases. ...
Journal of Molecular Evolution
Journal of Molecular Evolution

... lation geneticists and empirical evidence has been provided for it by molecular biologists working with hybrid phages (Furth and Yates 1978; Susskind and Botstein 1978), from reversion studies of bacterial mutants (Ebright et al. 1985), and from interspecific hybrids of the mammalian cytochrome chai ...
Document
Document

... The strand of DNA that would produce a mirror image (antisense) messenger RNA that is opposite in sequence to one directing protein synthesis. Antisense technology is used to selectively turn off production of certain proteins. Antiserum. Blood serum containing specific antibodies against an antigen ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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