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PCR: an outstanding method
PCR: an outstanding method

Promoter Analysis for Intestinally
Promoter Analysis for Intestinally

RecQ-like helicases and the DNA replication checkpoint
RecQ-like helicases and the DNA replication checkpoint

... ‡Author for correspondence (e-mail: [email protected]) ...
The Schistosoma gene discovery program: state of the art
The Schistosoma gene discovery program: state of the art

... It is very dicult to keep Schistosoma in the laboratory. The complex life cycle can only be maintained by passage through invertebrate hosts (snails) and mammalian hosts (mouse or hamster). This fact restricts the availability of material from some stages for preparation of cDNA and genomic librari ...
University of Groningen Characterization of the lytic-lysogenic
University of Groningen Characterization of the lytic-lysogenic

... ORF76 at the amino acid level, binds the switch region from Tuc2009 (data not shown). In order to determine the sequences recognized by Cro2009 DNase I protection assays were performed on the DNA encoding the intergenic region in the presence of Cro2009 (Fig. 2D). Two protected regions were observed ...
Saccharomyces Genome Database.
Saccharomyces Genome Database.

... SHUAI WENG, DAVID BOTSTEIN,and J. MICHAELCHERRY Introduction The goal of the Saccharomyces Genome Database (SGD) is to provide information about the genome of this yeast, the genes it encodes, and their biological functions. The genome sequence of S. cerevisiae provides the structure around which in ...
Background and Overview of Comparative Genomics
Background and Overview of Comparative Genomics

... Petri dish. The vector carrying a particular sequence can then be isolated by cloning the progeny of a single virus, bacterium, or yeast. One variation is to start with messenger RNA prepared from a particular tissue, make DNA copies (cDNA) and splice them into a vector. These cDNA libraries provide ...
[ 19] Saccharomyces Genome Database - SGD-Wiki
[ 19] Saccharomyces Genome Database - SGD-Wiki

... SHUAI WENG, DAVID BOTSTEIN,and J. MICHAELCHERRY Introduction The goal of the Saccharomyces Genome Database (SGD) is to provide information about the genome of this yeast, the genes it encodes, and their biological functions. The genome sequence of S. cerevisiae provides the structure around which in ...
Prentice Hall Biology - Mid
Prentice Hall Biology - Mid

... The Good With the Bad The manipulation of DNA allows scientists to do some interesting things. Scientists have developed many transgenic organisms, which are organisms that contain genes from other organisms. Recently, scientists have removed a gene for green fluorescent protein from a jellyfish and ...
Exam #3 Part of Ch. 13, Ch.14-17 and Ch. 20 Supplement to notes
Exam #3 Part of Ch. 13, Ch.14-17 and Ch. 20 Supplement to notes

... 17.1 One gene- one enzyme versus One-gene one polypeptide hypothesis, Basics principles of transcription and translation, RNA processing, pre-mRNA, primary transcript, the genetic code, codon, triplet code, template strand, template, nontemplate strands, translation read from 5’ to 3’, reading frame ...
tRNA and Protein Building
tRNA and Protein Building

... ribosomes. This RNA is a specific sequence of base copied from the DNA which carries the chromosomal genetic message to the cytoplasm. Thus, it is called messenger RNA (mRNA). At the ribosomes, mRNA directs the building of proteins. Proteins are made up of smaller molecules called amino acids. How d ...
Organization of Project
Organization of Project

... you provided, to make sure that there was at least one time you all could meet. You can change that time (or supplement it), so long as there is one time that does not conflict with the times of others (so that I can attend the meeting). ...
S1.An RFLP marker is located 1 million bp away from a gene of
S1.An RFLP marker is located 1 million bp away from a gene of

... S1.An RFLP marker is located 1 million bp away from a gene of interest. Your goal is to start at this RFLP marker and walk to this gene. The average insert size in the library is 55,000 bp and the average overlap at each end is 5,000 bp. Approximately how many steps will it take to get there? Answer ...
Examining the Process of de Novo Gene Birth
Examining the Process of de Novo Gene Birth

... One final introductory note: while the Abrusán (2013) article is indeed hypothesis-driven research, it may seem a bit different from what most people think of as experimental ...
Document
Document

... S1.An RFLP marker is located 1 million bp away from a gene of interest. Your goal is to start at this RFLP marker and walk to this gene. The average insert size in the library is 55,000 bp and the average overlap at each end is 5,000 bp. Approximately how many steps will it take to get there? Answer ...
Common DNA sequences with potential for detection of genetically
Common DNA sequences with potential for detection of genetically

... markers used in laboratory construction of plant expression systems may be eliminated from genetically modified plants prior to their application in the field. Herbicide resistance markers (Table 1) are less likely than antibiotic markers to be removed from plant cells prior to release into the fiel ...
Microbial Ecology
Microbial Ecology

... endophytes has been stimulated by their potential use for plant-growth promotion, antagonistic effects against plant pathogens, or biological nitrogen fixation for sustainable agriculture [13]. Although several graminaceous plants have been investigated for the occurrence and diversity of endophytic ...
Introduction to Molecular Markers and their
Introduction to Molecular Markers and their

... •SSR-a site in the genome that contains many short tandem repeat sequences (microsatellites). These sites are usually in the size range of 100-500 base pairs composed of dinucleotide and trinucleotide repeats. They are very polymorphic, scattered through out genomes. Genomes typically contain 1,000s ...
you can view a sample report here.
you can view a sample report here.

... Variants Found production of neurotransmitters, reduced conversion of homocysteine to methionine, and reduced s-adenosylMultiple have considered riboflavin and shown that the MTHFR 677that TTWhen genoMTRR studies MTRR attaches a methyl group tostatus B12 considered and variants here will slow thesho ...
TARGETING YOUR DNA WITH THE CRE/LOX SYSTEM
TARGETING YOUR DNA WITH THE CRE/LOX SYSTEM

... front of the gene that functions to recruit the cellular machinery that will initiate the multi-step process of protein production (called gene expression). How the promoter functions to do this can vary, from always recruiting cellular machinery and thus always being ‘on’, to only doing this in spe ...
Sample pages 2 PDF
Sample pages 2 PDF

... single nucleotide polymorphisms (SNPs) and copy number variations (CNVs). SNPs refer to specific positions in a chromosome where different nucleobases are observed, the result of a so-called point mutation. Copy number variation refers to relatively long stretches of DNA which are repeated a differe ...
Producing a Recombinant Plasmid, pARA-R
Producing a Recombinant Plasmid, pARA-R

... molecules because the four restriction fragments have been recombined in different ways to produce new constructs. For example, assume that the four plasmid fragments were represented by the letter A, A’, K and R, where A and A’ represent the pARA fragments and K and R represent the two fragments re ...
Leukaemia Section t(2;9)(p23;q33) TRAF1/ALK Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(2;9)(p23;q33) TRAF1/ALK Atlas of Genetics and Cytogenetics in Oncology and Haematology

... Result of the chromosomal anomaly ...
A mutation in the Zn-finger of the GAL4
A mutation in the Zn-finger of the GAL4

... derepression of the gene in the absence of a fermentable carbon source (8). Induction of the gene in response to oxygen is controlled by different transcription factors, HAP1 and RC2 which bind to the adjacent UAS 1 element (9). Here we present an example in which a single transcription factor LAC9 ...
Chapter 7 Microbial Genetics
Chapter 7 Microbial Genetics

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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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