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4132010
4132010

... A form of post-transcriptional gene silencing, mimicking the effect of loss-of-gene-function. ...
Qβ replicase discriminates between legitimate and illegitimate
Qβ replicase discriminates between legitimate and illegitimate

... thermodynamically more stable than are the intrastand secondary structures: If a mixture of complementary is annealed (melted and then slow cooled), they are completely converted into double helix. • Within the replicative complex, the template and the nascent strands are close to one another, which ...
Biology 1060 Chapter 17 - College of Southern Maryland
Biology 1060 Chapter 17 - College of Southern Maryland

... Describe the three stages of transcription Discuss the factors important in RNA polymerase binding and initiation of transcription Discuss how the cell increases the efficiency of transcription to mRNA Describe termination in prokaryotes and eukaryotes ...
Supplementary Methods
Supplementary Methods

... Comparison with the Accuracy of the MAQC microarray results. In the main text Fig. 3b shows a comparison of the ExpressSeq and QRTPCR measurements of the fold-changes (on log base 2 scales) between the A and B sample for the 217 genes found to be “present” in the MAQC TaqMan assays and on all of the ...
The S RNA segment of tomato spotted wilt virus has an ambisense
The S RNA segment of tomato spotted wilt virus has an ambisense

... open reading frames (ORFs), one on each strand. No additional ORFs of significant length (i.e. ORFs encoding proteins larger than 5K) could be detected in any of the other reading frames (Fig. 4). The O R F on the viral R N A strand starts with an A U G codon at position 88 and terminates at an UAA ...
Gill: Genes Enrichment, Gene Regulation I
Gill: Genes Enrichment, Gene Regulation I

... • Promoter: The region of DNA 100-1,000bp immediately “upstream” of the TSS, which encodes binding sites for the general purpose RNA polymerase associated TFs, and at times some context specific sites. – There are as many promoters as there are TSS’s in the human genome. Many genes have more than on ...
Tumor-Suppressor Genes
Tumor-Suppressor Genes

... condensed than that of mitotic chromosomes • Much of the interphase chromatin is present as a 10-nm fiber, and some is 30-nm fiber, which in some regions is folded into looped domains ...
Leukaemia Section t(1;21)(p32;q22) Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(1;21)(p32;q22) Atlas of Genetics and Cytogenetics in Oncology and Haematology

... Chromosome and FISH images showing : 1) partial karyotype and ideogram of t(1;21)(p32;q22) including a second copy of the der(1)t(1;21) present in the clone; and 2) metaphase FISH showing red AML1 signal on the two copies of the der(t)t(1;21), the der(21)t(1;21) and the normal 21 homolog. Green TEL ...
The Human Globin Genes
The Human Globin Genes

... Other Repetitive DNA, Including Simple Sequence DNA • About 15% of the human genome consists of duplication of long sequences of DNA from one ...
Genomes
Genomes

... Other Repetitive DNA, Including Simple Sequence DNA • About 15% of the human genome consists of duplication of long sequences of DNA from one ...
Genome Annotation
Genome Annotation

... sequences that are important components of the genome. These sequences include the ribosomal RNAs and tRNAs that are essential for protein translation. In addition, the small nuclear RNAs important to processes such as RNA splicing are necessarily components of the gneome. These sequences exhibit a ...
Chapter 21
Chapter 21

... Concept 21.4: Multicellular eukaryotes have much noncoding DNA and many multigene families • The bulk of most eukaryotic genomes neither encodes proteins nor functional RNAs ...
Chapter 15 Guided Reading
Chapter 15 Guided Reading

... Section 15.2: Eukaryotic Gene Expression Another long one! Good video to watch for this next section: http://www.youtube.com/watch?v=kqFa5xgg9wc 1. What fractions of protein coded genes are typically expressed in a human cell at a time? _______ 2. What about muscle and nerve cells? ...
iCLIP HeLa cells were UV crosslinked before lysing in lysis buffer
iCLIP HeLa cells were UV crosslinked before lysing in lysis buffer

... proceeding to further analysis. (iii) Control file with random placement of iCLIP reads on corresponding genes was generated 100 times. Each 5’UTR, 3’ UTR, and each intron is its own region; all remaining parts of the gene are its own region (these will be all exononic sequences corresponding to OR ...
transcription factor
transcription factor

... • Loops of chromatin extend from individual chromosomes into specific sites in the nucleus • Loops from different chromosomes may congregate at particular sites, some of which are rich in transcription factors and RNA polymerases • These may be areas specialized for a common ...
w latach 2016-2018 na Wydziale Biologii Uniwersytetu im. Adama
w latach 2016-2018 na Wydziale Biologii Uniwersytetu im. Adama

... material known as meiotic recombination or crossover (CO). This process is required for proper chromosome segregation, therefore it is obligatory for each chromosome pair. CO is also the basic source of genetic variation within natural populations as it creates new arrangements of alleles. For this ...
19. Gene Regulation
19. Gene Regulation

... I gene is transcribed and translated to produce repressor Repressor binds inducer (form of lactose) Conformation of repressor is altered Repressor can’t bind operator RNA polymerase can bind promoter and transcribe genes in the operon (ZYA) The polycistronic mRNA is translated to give β-galactosidas ...
problem set
problem set

... of the transcript at the cleavage and polyadenylation site in the fifth intron. However, the splicing of the fifth intron is efficient in muscle cells. This removes the cleavage and polyadenylation signal and results in a longer spliced mRNA containing all 10 exons. A muscle-specific splicing factor ...
Fig. 17.1 Levels at which gene expression can be controlled in
Fig. 17.1 Levels at which gene expression can be controlled in

... • What evidence has shown the role of chromosome packaging and histone proteins in gene regulation? • What role does DNA methylation play? • What are DNA binding motifs in transcription factor proteins? • What are enhancers and silencers? • How does RNA processing and stability contribute to gene re ...
Do the constraints of human speciation cause
Do the constraints of human speciation cause

... necessity due to the constraints of the speciation process. (1) Even at the evolutionary state of the early vertebrate, the genome was well balanced and had reached a high degree of complexity. No gross additions (or deletions) of genes have occurred since that time (Wakefield and Graves, 1996). The ...
Genetics Journal Club - Perelman School of Medicine at the
Genetics Journal Club - Perelman School of Medicine at the

... Allele specific variants are defined using histone acetylation, H3K9me3, H3K27me3, DHS and H3K4me3 h) Number of allele-biased genes showing consistent allele specific chromatin states in their promoter regions. Active variants are defined by H3K4me3, DHS or histone acetylation. Inactive promoter var ...
Document
Document

... funded by the Wellcome Trust. • Goals of Ensembl • The Ensembl project aims to provide: • Accurate, automatic analysis of genome data. • Analysis and annotation maintained on the current data. • Presentation of the analysis to all via the web. • Distribution of the analysis to other bioinformatics l ...
Microbial Minimalism: Genome Reduction in Bacterial Pathogens
Microbial Minimalism: Genome Reduction in Bacterial Pathogens

... the opportunity to reconstruct the process of genome reduction. Such an attempt to reconstruct the pattern of gene deletions during the evolution of Buchnera suggested that, in addition to gradual erosion of some individual genes through small deletions, some deletions were large and spanned dozens ...
Document
Document

... Initial sequence annotations of the human genome have uncovered at least 32,000 genes (International Human Genome Sequencing Consortium 2001), or 26,000–39,000 genes (Venter et al. 2001). The mean gene size is thought to be 27 kb. Although these gene count estimates are acknowledged, by the authors ...
Poster
Poster

... In alternative splicing, precursor messenger RNA is processed to produce many different messenger RNAs. The expression of these different RNAs from one gene makes possible the enormous protein diversity found in humans. Alternative splicing affects over 90 percent of our genome, allowing humans to b ...
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Short interspersed nuclear elements (SINEs)

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