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Ch11_lecture - Dr Owen class material
Ch11_lecture - Dr Owen class material

... • Each ribosome consists of two subunits—one small and one large. • The small subunit has binding sites for mRNA, a “start” tRNA, and other proteins that cooperate to read mRNA to start protein synthesis. • The large subunit has two binding sites for tRNA molecules, and one catalytic site where pept ...
Chapter Sixteen: Control of Gene Expression
Chapter Sixteen: Control of Gene Expression

... structural genes will not occur. The hairpin structure formed by the pairing of region 3 with region 4 results in a terminator being formed that stops transcription. When region 2 pairs with region 3, the resulting hairpin acts as an antiterminator allowing for transcription to proceed. Region 1 of ...
mv-lect-06-virus-repl-stratigies
mv-lect-06-virus-repl-stratigies

... These viruses replicate their genomes via one of two unique biochemical pathways: • RNA-dependent RNA synthesis (RNA replication), • RNA-dependent DNA synthesis (reverse transcription) followed by DNA replication and transcription. Both pathways require enzyme activities that are not usually found i ...
Genome Assembly and Annotation
Genome Assembly and Annotation

... – ~ 45% of the human genome consists of repeats interspersed with non-repetitive sequences • Transposon derived (LINEs, SINEs) • 3 – 4% segmental duplications (> 1kb, > 90% similar), ~ 40% believed to be misassembled • Multi-gene families ...
Document
Document

... • This loosens chromatin structure, thereby promoting the initiation of transcription • The addition of methyl groups (methylation) can condense chromatin; the addition of phosphate groups (phosphorylation) next to a methylated amino acid can loosen chromatin Animation: DNA Packing © 2011 Pearson Ed ...
Individual eukaryotic genomes
Individual eukaryotic genomes

... Distinguishing features: It is the malaria parasite vector. Genome size: 278 Mb (twice the size of Drosophila) Chromosomes: 3 Genes: about 14,000 Website: http://www.ensembl.org/Anopheles_gambiae/ --Diverged from Drosophila 250 MYA (average amino acid sequence identity of orthologs is 56%). Compare ...
Health and Technology
Health and Technology

... Linkage & Recombination By the end of this lesson you should be able to: Know what is meant by linked genes. Know the consequences of genes being linked. Understand the effect crossing over has on linked genes. Know the effect that crossing over has on distantly located genes. Know what cross- ...
Yu-GO
Yu-GO

... has many strongly correlated genes from elsewhere of the genome; • but these genes are not highly correlated within themselves, and their cellular roles are diverse. • For instance, when we submit the top 200 genes which have the best correlations (all >0.57) with ‘NAD biosynthesis’ to GO Term Finde ...
RNAi and RNAa - The Yin and Yang of RNAome
RNAi and RNAa - The Yin and Yang of RNAome

... Figure 1: RNAa/RNAi constitutes the Yin and Yang of the RNAome. The dual concepts of yin and yang which describe two primal opposing but complementary principles or cosmic forces said to be found in all non-static objects and processes in the universe. This paradoxical concept can potentially be app ...
Document
Document

... For a given gene, only one strand of the DNA serves as the template for transcription. An example is shown below. The bottom (blue) strand in this example is the template strand, which is also called the minus (-) strand,or the sense strand. It is this strand that serves as a template for the mRNA s ...
Figure 5.x3 James Watson and Francis Crick
Figure 5.x3 James Watson and Francis Crick

... The mRNA then enters the cytoplasm and attaches to a ribosome. Translation begins at AUG, the start codon. Each transfer RNA has an anticodon whose bases are complementary to a codon on the mRNA strand. The ribosome positions the start codon to attract its anticodon, which is part of the tRNA that b ...
Ensembl gene annotation project (e!76) Homo sapiens (human
Ensembl gene annotation project (e!76) Homo sapiens (human

... merged gene set in the future. As for supporting evidence, the merge of Ensembl and HAVANA transcripts also involved merging of protein and cDNA supporting evidence associated with the transcripts to ensure the basis on which the annotations were made would not be lost. Following the merge, the long ...
Using variability in gene expression as a tool for studying
Using variability in gene expression as a tool for studying

... expression are RNA knockdown [via small interfering RNA(siRNA), small hairpin RNA(shRNA), antisense oligonucleotides (ASO), RNAi, or morpholino, among other methods] and transgenic overexpression (with other new methods under development19,20 ). The former methods often suffer from the fact that the ...
2017 Reg of Mitosis Genetics Protein Synth Regulation Review
2017 Reg of Mitosis Genetics Protein Synth Regulation Review

... period of time and compare and contrast euchromatin and heterochromatin Be able to compare general transcription factors and specific transcription factors including purpose and place of DNA attachment. Be able to explain the purpose of enhancers, silencers, activators, repressors, DNA bending prote ...
Gene expression regulation and the lactase gene
Gene expression regulation and the lactase gene

... - human: lactase production usually drops about 90% during the first four years of life (varies widely), but there are human populations which tolerate fresh milk and other dairy products throughout their lives (lactase persistence) ...
to get the file - Chair of Computational Biology
to get the file - Chair of Computational Biology

... Using primary sequence similarity of the H. salinarum proteins to characterized orthologs in other organisms left a significant fraction (38%) of ~2,400 putative protein-coding genes that could not be assigned any function. To overcome this hurdle, we applied a more sensitive approach that incorpora ...
ppt - Chair of Computational Biology
ppt - Chair of Computational Biology

... Using primary sequence similarity of the H. salinarum proteins to characterized orthologs in other organisms left a significant fraction (38%) of ~2,400 putative protein-coding genes that could not be assigned any function. To overcome this hurdle, we applied a more sensitive approach that incorpora ...
Clock-Controlled Genes
Clock-Controlled Genes

... (AVP) had been known before as rhythmically expressed genes in the SCN. Another important set of coordinately expressed genes contained enzymes important for carbon source utilization and oxidative phosphorylation in the mitochondria. The detailed analysis of this pathway suggested a circadian rhyth ...
AnnotatorsInterface-GUS
AnnotatorsInterface-GUS

... Provide alignment of features on genomic sequence – could potentially display any feature type currently stored in GUS3.0 – features can be selected and used to generate “curated” features – similar to display and functionality in Apollo ...
Genetic Manipulation of Kinetoplastida
Genetic Manipulation of Kinetoplastida

... quences present in the input plasmid, as results of one the mutant with the gene that had been deleted. study suggested that the minimum size for a stable linInducible expression. Both over- and underexpression ear chromosome was about 50 kb21 whereas, in another of a gene product can be lethal. In ...
Clustering2_11-8
Clustering2_11-8

... Is it possible that some of these gene expression changes are miscalled (i.e. biologically significant but insignificant p value and vice versa) and why? What other criteria might you use to distinguish genes you care about? How many genes pass the cutoff of q<0.01 and how does this compare to the n ...
279 - aaabg
279 - aaabg

... and Parasite:L21 genes (bottom right) clustering of expression levels of the Parasite:L7 (Figure 2). The parasite: L7 heat plot shows 2 distinct gene clusters: 1) those more up regulated in Tc L7 and 2) those more up regulated in Hc L7. This indicates the presence of unique co-expressed gene modules ...
Genomic evidence for ameiotic evolution in the bdelloid
Genomic evidence for ameiotic evolution in the bdelloid

... absent from the genomes of asexuals18 or undergo unrestrained expansion after the switch to asexuality, potentially leading to species extinction unless transposable element proliferation is prevented19. We found that transposable elements cover about 3% of the A. vaga genome, which is less than the ...
File
File

... rates of crossing-over between genes in meiosis might be a clue to something important. Sturtevant reasoned that the farther apart two genes were, the more likely they were to be separated by a crossover in meiosis. That meant that he could use recombination frequencies to determine the distances be ...
Pseudogenes as regulators of biological function
Pseudogenes as regulators of biological function

... The regulation of mRNA stability is one way in which gene expression can be controlled. The stability of an mRNA can be influenced by protein factors that bind at different locations in the RNA [38]. If a pseudogene has a high homology with the parent coding gene, including the presence of the same ...
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Short interspersed nuclear elements (SINEs)

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