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ppt - Chair of Computational Biology
ppt - Chair of Computational Biology

... This suggests that many TFs can be regulated simply by the abundance (expression levels) of the TF. However, across 1000 microarray expression experiments for yeast, the correlation between a TF’s expression and that of its ChIP-based targets was typically very low (only between 0 and 0.25). Conside ...
Repression of the Defense Gene PR-10a by the Single
Repression of the Defense Gene PR-10a by the Single

... The potato pathogenesis-related gene PR-10a is transcriptionally activated in response to pathogen infection or elicitor treatment. Characterization of the cis-acting elements of the PR-10a promoter revealed the presence of a silencing element between residues 52 and 27 that contributes to transcr ...
Characteristic Features of the Nucleotide Sequences of Yeast
Characteristic Features of the Nucleotide Sequences of Yeast

... have recently become available. In addition, the data for indicated. All four genes were predicted to be likely genes chromosomes III and V were extracted from the Gen- when the sc_cul.5 (order 5) matrix was used (Fig. la). In Bank database and analyzed. In the annotation lines contrast, when the MR ...
Transposable elements, genes and recombination in a 215
Transposable elements, genes and recombination in a 215

... a genome designated Am that is closely related to the genome of T. urartu, the A genome donor for tetraploid and hexaploid wheats. The large genome of T. monococcum (1C =5600 Mb; Bennett and Leitch 1995) is approximately 12 times larger than the genome of rice and 40 times larger than the genome of ...
Diapositiva 1
Diapositiva 1

... A non-redundant collection of richly annotated DNA, RNA, and protein sequences from diverse taxa The collection includes sequences from plasmids, organelles, viruses, archaea, bacteria, and eukaryotes Each RefSeq represents a single, naturally occurring molecule from one organism. RefSeq biological ...
PDF
PDF

... Genomic imprinting is an epigenetic phenomenon in mammals whereby the expression of a subset of autosomal genes is restricted to one of the parental chromosomes such that they are expressed either from the maternal or the paternal chromosome. So far more than 80 imprinted genes have been identified ...
Regulatory sequences
Regulatory sequences

... and Y sequences conserved in all MHC-II, Ii, and HLA-DM promoters. RFX, X2BP, NF-Y, and an as yet undefined W-binding protein bind cooperatively to these sequences and assemble into a stable higher order nucleoprotein complex referred to here as the MHC-II enhanceosome. CIITA is tethered to the enha ...
Protein Synthesis powerpoint
Protein Synthesis powerpoint

... • Splicing itself may regulate the passage of mRNA from the nucleus to the cytoplasm. • One clear benefit of split genes is to enable a one gene to encode for more than one polypeptide. ...
file
file

... Examined intergenic regions of human mouse rate and dog ~18,000 genes “Promoters”: 4kb centered on TSS 3UTRs based on RNA annotations 64 Mb, and 15 Mb in total respectively Negative control: Introns of ~120 Mb % of alignable sequence: promoters: 51% (44% upstream and 58% downstream of the TSS), 3’ U ...
Structure and Transcription of the singed Locus of Drosophila
Structure and Transcription of the singed Locus of Drosophila

... transcription of singed in the ovary is from middle to late stages of oogenesis. Analysis of RNA in embryos from the reciprocal crosses between wild type and singed-3 showed that all three RNAs are maternally inherited with very little zygotic transcription in embryos. The mutation singed3 appears t ...
ab initio - Ware Lab
ab initio - Ware Lab

... Now in its third year, the project has produced complete sequences of 15,200 BAC clones comprising approximately 2 billion non-redundant bases, all available via GenBank. Annotation of this first draft, using both ab initio gene prediction and evidence-based approaches, gives preliminary estimates o ...
MIT 2006: Engineering bacteria to smell good
MIT 2006: Engineering bacteria to smell good

... Need: To specifically control who can read the DNA message Means: Riboregulation Some slides borrowed from the 2006 Berkeley Team ...
Evolution of Gene Expression
Evolution of Gene Expression

... mechanisms controlling transcriptional and posttranscriptional regulation of gene expression. Within prokaryotes and eukaryotes, these mechanisms are highly conserved, but they differ significantly between the two groups. The remainder of this chapter focuses solely on transcriptional regulation in ...
pdf
pdf

... middle of mRNAs than the 5’ ends. Thus it is common to have access to this part of the cDNA, and that provides the sequence information for the second, or internal, primer. In contrast, specialized techniques are often employed to get information about the 5’ ends of mRNAs. In the technique outlined ...
Plant Gravitational and Space Genomics Tour
Plant Gravitational and Space Genomics Tour

... We have carried out microarray analysis of Arabidopsis root tips after gravistimulation (reorientation) and mechanical stress and monitored transcript levels ca. 24,000 genes during the first hour after either stimuli. We identified genes responding to either stimulus specifically as well as genes s ...
Nature Biotechnology, 21(4) - Weizmann Institute of Science
Nature Biotechnology, 21(4) - Weizmann Institute of Science

... several years in a variety of eukaryotic organisms2–5. Antisense transcripts often code for proteins involved in diverse biological functions. Noncoding antisense transcripts have also been identified. Their role appears to be mainly regulatory6 and their transcription is often associated with genom ...
The Sexual Nature of the Eukaryote Genome
The Sexual Nature of the Eukaryote Genome

... fusion. (2) Conjugative plasmids permit gene transfer between prokaryotes and eukaryotes, and can be integrated into the eukaryote genome. (3) Genes can be transferred between unrelated eukaryotes, and between different genomes within eukaryotic cells. (4) Elements such as transposons and retrovirus ...
Epigenetics in Yeast
Epigenetics in Yeast

... So H3K9me by Clr4 would repress pho1, contradicts ChIP result. ...
University of Groningen Characterisation of actinomycete
University of Groningen Characterisation of actinomycete

... that does not display similarity to any previously known replication proteins ...
Nuclear Matrix Proteins and Nuclear Targeting
Nuclear Matrix Proteins and Nuclear Targeting

... there is growing evidence that many nuclear proteins contain an Nuclear Targeting Sequence (NTS’s) that target individual proteins to the sites of genomic function/organization. • A classic example is the DNA methyl transferase (MTase) which is an enzyme associated with replication sites in cells an ...
Reading frame
Reading frame

... Eukaryotic Genome: •Non-coding DNA including introns, •Exon=coding DNA •Gene=Exons+Introns ...
M2 RNA Pol Ⅰ genes
M2 RNA Pol Ⅰ genes

... They typically lie upstream of the gene and can have regulatory elements several kilobases away from the transcriptional start site. (Fig.11.19) ...
DNA constructs designed to produce short hairpin, interfering RNAs
DNA constructs designed to produce short hairpin, interfering RNAs

... Abstract. Arylamine N-acetyltransferase (NAT) genes were targeted for inhibition using short hairpin RNA (shRNA) using two different RNA polymerase III promoters. Constructs were developed for NAT1 and NAT2, the endogenous mouse genes, and for human NAT1. There were fetal and neonatal deaths with th ...
Regulation of biosynthesis and transport of aromatic amino acids in
Regulation of biosynthesis and transport of aromatic amino acids in

... Complete genome sequences of B. subtilis, B. halodurans, Streptococcus pneumoniae, L. lactis, Streptococcus pyogenes, Clostridium acetobutylicum, Staphylococcus aureus, and Listeria monocytogenes were downloaded from GenBank (http://www.ncbi.nlm.nih.gov). Partially sequenced genomes of B. stearother ...
Tutorial for Interpretation of T-REx Results
Tutorial for Interpretation of T-REx Results

... Goal: Find genes that connects contrasts (the cohesion of contrasts). This alternative for Venn Diagrams provides a clear overview of the number of genes shared by contrasts or that are specific for one contrast. The list of genes can be easily downloaded via a direct link in the cohesion of contras ...
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Short interspersed nuclear elements (SINEs)

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