p53 regulation and function in normal cells and tumors
... consequences of such exposure include the pathway of the tumor suppressor p53 protein, a guardian of the genome. p53 is a DNA binding protein; attention has focused on its specific binding to a consensus sequence within promoter regions of growth inhibitory genes. However, p53 also binds sequence-in ...
... consequences of such exposure include the pathway of the tumor suppressor p53 protein, a guardian of the genome. p53 is a DNA binding protein; attention has focused on its specific binding to a consensus sequence within promoter regions of growth inhibitory genes. However, p53 also binds sequence-in ...
Age and menopause affect the expression of
... One of the great successes of modern society is the global increase in life expectancy; in part due to greater control of infectious diseases, better living conditions, better nutrition and new medical technologies [1-3]. Furthermore, the gap between the life expectancy of populations in developed a ...
... One of the great successes of modern society is the global increase in life expectancy; in part due to greater control of infectious diseases, better living conditions, better nutrition and new medical technologies [1-3]. Furthermore, the gap between the life expectancy of populations in developed a ...
Full Text - PDF - Donnish Journals
... A total of 50 blood samples of active visceral leishmaniasis was identified clinically by fever of more than two-week duration, hepatosplenomegaly, and pancytopenia then confirmed by rK39 Dipstick. Fifty bone marrow smears showed moderate to sever megaloblastosis, an increased number of plasma cells ...
... A total of 50 blood samples of active visceral leishmaniasis was identified clinically by fever of more than two-week duration, hepatosplenomegaly, and pancytopenia then confirmed by rK39 Dipstick. Fifty bone marrow smears showed moderate to sever megaloblastosis, an increased number of plasma cells ...
PDF - Paragon Bioservices
... production of cytokines and chemokines that direct inflammation and promote the induction of adaptive immunity. Accordingly, exposure to flagellin, either by natural infection or immunization, results in high levels of serum anti-flagellin antibodies and robust CD4+ T-cell responses [4,15,16]. The powe ...
... production of cytokines and chemokines that direct inflammation and promote the induction of adaptive immunity. Accordingly, exposure to flagellin, either by natural infection or immunization, results in high levels of serum anti-flagellin antibodies and robust CD4+ T-cell responses [4,15,16]. The powe ...
Scenario 3 - People.vcu.edu
... 1533628 (start-TTG) Length = 301 amino acids IC: Inferred by curator Use this when your assertion is based on your own judgement Function: PvuII DNA methyltransferaseof sequence similarity or other information, when there is no direct experimental data. Cyanobacterial orthologs: none ...
... 1533628 (start-TTG) Length = 301 amino acids IC: Inferred by curator Use this when your assertion is based on your own judgement Function: PvuII DNA methyltransferaseof sequence similarity or other information, when there is no direct experimental data. Cyanobacterial orthologs: none ...
Blockade of ASC but not NLRP3 Inhibits DC Proliferation and T cell
... Dendritic cells are professional APCs capable of stimulating a potent T cell response to alloantigen. Pro-inflammatory signals from innate immune pattern recognition receptors (PRRs) on donor DCs are thought to be key initiators of T cell responses [1-4]. The PRRs, such as toll-like receptors (TLRs) ...
... Dendritic cells are professional APCs capable of stimulating a potent T cell response to alloantigen. Pro-inflammatory signals from innate immune pattern recognition receptors (PRRs) on donor DCs are thought to be key initiators of T cell responses [1-4]. The PRRs, such as toll-like receptors (TLRs) ...
Assembly of additional heterochromatin distinct from centromere
... fragment (SV40-bsr cassette) with SV40 early promoter (including the enhancer element) and bsr gene from pSV2bsr (Kaken, Japan) was inserted into the SalI site of pBluescript II (Stratagene) after endfilling reactions. An XhoI and SalI fragment containing the SV40-bsr cassette from the resultant pla ...
... fragment (SV40-bsr cassette) with SV40 early promoter (including the enhancer element) and bsr gene from pSV2bsr (Kaken, Japan) was inserted into the SalI site of pBluescript II (Stratagene) after endfilling reactions. An XhoI and SalI fragment containing the SV40-bsr cassette from the resultant pla ...
Lymphatic/Immune Power Point
... • T cells: cell-mediated immunity – Killer T cells: punch holes in pathogen’s cell membrane and secrete lymphokines that enhance phagocytic activity – Helper T cells: secrete a lymphokine that stimulates T cells and B cells and enhances immune response – Suppressor T cells: inhibit immune response w ...
... • T cells: cell-mediated immunity – Killer T cells: punch holes in pathogen’s cell membrane and secrete lymphokines that enhance phagocytic activity – Helper T cells: secrete a lymphokine that stimulates T cells and B cells and enhances immune response – Suppressor T cells: inhibit immune response w ...
B7-1, B7-2 and class II MHC molecules in idiopathic pulmonary
... epithelial cells, as well as alveolar macrophages in lung tissues from patients with IPF (fig. 1A and B). In normal lung parenchyma, positive signals were found in macrophages but not in epithelial cells (fig. 1C). As shown in figure 2, the degree of B7-1 expression in bronchiolar and alveolar epith ...
... epithelial cells, as well as alveolar macrophages in lung tissues from patients with IPF (fig. 1A and B). In normal lung parenchyma, positive signals were found in macrophages but not in epithelial cells (fig. 1C). As shown in figure 2, the degree of B7-1 expression in bronchiolar and alveolar epith ...
Yeast genetics to investigate the function of core pre
... (snRNAs) (see chapters by Lührmann and Rymond). Compositions of both human and yeast core spliceosomes are now known, and many protein factors and all the snRNAs are evolutionarily conserved. Yeast have been used to investigate mutations in core spliceosome factors known to cause disease in humans [ ...
... (snRNAs) (see chapters by Lührmann and Rymond). Compositions of both human and yeast core spliceosomes are now known, and many protein factors and all the snRNAs are evolutionarily conserved. Yeast have been used to investigate mutations in core spliceosome factors known to cause disease in humans [ ...
Biology
... During translation, the cell uses information from messenger RNA to produce proteins and tell what order they should be listed in on the polypeptide. ...
... During translation, the cell uses information from messenger RNA to produce proteins and tell what order they should be listed in on the polypeptide. ...
University of Groningen DNA-based asymmetric catalysis
... with enzymes. Although small molecule catalysts can have high catalytic activity, they are generally still outperformed by enzymes. However, the possibility to catalyze a wide variety of reactions, renders small molecule catalysts very important for applications of asymmetric catalysis in industry,8 ...
... with enzymes. Although small molecule catalysts can have high catalytic activity, they are generally still outperformed by enzymes. However, the possibility to catalyze a wide variety of reactions, renders small molecule catalysts very important for applications of asymmetric catalysis in industry,8 ...
The phenotype of alveolar macrophages ... with immune cells in bronchoalveolar ...
... Distinct phenotypic subpopulations of AMs have recently been identified [15, 16]. Macrophages with properties of dendritic, phagocytic or suppressive cells may be separated on the basis of the eo-expression of membrane antigens RFD1, RFD7 and RFD9 [17, 18]. Other subpopulations of AMs can be disting ...
... Distinct phenotypic subpopulations of AMs have recently been identified [15, 16]. Macrophages with properties of dendritic, phagocytic or suppressive cells may be separated on the basis of the eo-expression of membrane antigens RFD1, RFD7 and RFD9 [17, 18]. Other subpopulations of AMs can be disting ...
Deprivation of protein or amino acid induces C/EBPβ synthesis and
... fluctuations in intracellular pools, a number of dietary and pathological conditions can result in decreased intracellular amino acid availability, which in turn can modulate a number of fundamental processes. Under these circumstances, the amino acids are not only serving their role as metabolic or ...
... fluctuations in intracellular pools, a number of dietary and pathological conditions can result in decreased intracellular amino acid availability, which in turn can modulate a number of fundamental processes. Under these circumstances, the amino acids are not only serving their role as metabolic or ...
Immune diseases And Children
... Immunity depends on an intricate homeostatic system aimed at maintaining a delicate balance between health and disease. Its function is maintained by a series of complex, highly regulated, multi-cellular, physiologic mechanisms designed to accomplish a singular goal: to differentiate self from non-s ...
... Immunity depends on an intricate homeostatic system aimed at maintaining a delicate balance between health and disease. Its function is maintained by a series of complex, highly regulated, multi-cellular, physiologic mechanisms designed to accomplish a singular goal: to differentiate self from non-s ...
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... detecting codons that have undergone positive selection, where dS is the number of synonymous substitutions per site (s/S) and dN is the number of nonsynonymous substitutions per site (n/N). A ...
... detecting codons that have undergone positive selection, where dS is the number of synonymous substitutions per site (s/S) and dN is the number of nonsynonymous substitutions per site (n/N). A ...
Carbohydrate Vaccines
... Therefore, the development of new vaccines to combat these types of infections is very important. Vaccines have generally been made from weakened or killed pathogens, or from several variable carbohydrate molecules, which are extracted from the cell-surface of bacteria and viruses. The carbohydrate- ...
... Therefore, the development of new vaccines to combat these types of infections is very important. Vaccines have generally been made from weakened or killed pathogens, or from several variable carbohydrate molecules, which are extracted from the cell-surface of bacteria and viruses. The carbohydrate- ...
Effects of Ozone Depletion
... sun. It is made up of wavelengths ranging from 100nm to 400nm. • UV radiation includes UV-A, the least dangerous form of UV radiation, with a wavelength range between 315nm to 400nm, UV-B with a wavelength range between 280nm to 315nm, and UV-C which is the most dangerous between 100nm to 280nm. UV- ...
... sun. It is made up of wavelengths ranging from 100nm to 400nm. • UV radiation includes UV-A, the least dangerous form of UV radiation, with a wavelength range between 315nm to 400nm, UV-B with a wavelength range between 280nm to 315nm, and UV-C which is the most dangerous between 100nm to 280nm. UV- ...
The DNA chromatin condensation expressed by the image optical
... central and peripheral regions might be related to different gene expression and cell differentiation (Gregoryev et al. 2006, Finlan et al. 2008, Kumaran and Spector 2008). On the other hand, the heterochromatin DNA IOD, i.e. the condensation state, was not compared with that in mitotic chromosomes ...
... central and peripheral regions might be related to different gene expression and cell differentiation (Gregoryev et al. 2006, Finlan et al. 2008, Kumaran and Spector 2008). On the other hand, the heterochromatin DNA IOD, i.e. the condensation state, was not compared with that in mitotic chromosomes ...
Effects of Mold Exposure on Immune Cells
... and incubated for 24 hours. Cell viability was tested at 30 minutes, 1 hour, 2 hours, 4 hours, 8 hours, and 24 hours. As expected, increasing VOC concentration was correlated with increased cell death, and longer incubation periods resulted in higher cell death percentages (Figure 2, Table 2). Effec ...
... and incubated for 24 hours. Cell viability was tested at 30 minutes, 1 hour, 2 hours, 4 hours, 8 hours, and 24 hours. As expected, increasing VOC concentration was correlated with increased cell death, and longer incubation periods resulted in higher cell death percentages (Figure 2, Table 2). Effec ...
Cloning a Gene for Over-expression and Purification
... follows a ribosome binding site and a transcription initiation site. (plasmid provides these). Extend the primer by 2-10 bases upstream of restriction site (√). Overlap with the desired gene for enough bases to give Tm ≈ 60 °C (72° preferred by some). ...
... follows a ribosome binding site and a transcription initiation site. (plasmid provides these). Extend the primer by 2-10 bases upstream of restriction site (√). Overlap with the desired gene for enough bases to give Tm ≈ 60 °C (72° preferred by some). ...
The Florida State University College of Arts and Sciences
... up regulated in both, mop1-1 and tgr1-1 mutants. Transposable elements (TEs) can influence the expression of nearby genes especially if located +/- 1kb of the gene’s start or end, which is associated with RNA-dependent de novo methylation, particularly CHH methylation (Gent et al. 2013; Lu et al. 20 ...
... up regulated in both, mop1-1 and tgr1-1 mutants. Transposable elements (TEs) can influence the expression of nearby genes especially if located +/- 1kb of the gene’s start or end, which is associated with RNA-dependent de novo methylation, particularly CHH methylation (Gent et al. 2013; Lu et al. 20 ...
DNA SEQUENCING (using a Li
... fragment, then the run time will be short (1-2 h). If the fragment is long, and the desired sequence is closer to the 3' end, then the run time will be longer (2-5 h). The other method of sequencing DNA (Sanger 1977) uses a polymerization reaction (using DNA polymerase) in conjunction with mixtures ...
... fragment, then the run time will be short (1-2 h). If the fragment is long, and the desired sequence is closer to the 3' end, then the run time will be longer (2-5 h). The other method of sequencing DNA (Sanger 1977) uses a polymerization reaction (using DNA polymerase) in conjunction with mixtures ...
DNA vaccination
DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.