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dna
dna

... Steps in Replication 2. A molecule of DNA POLYMERASE binds to one of the strands of DNA and begins to move in the 3’ to 5’ direction along it. This produces a new strand of DNA that is called the LEADING STRAND. DNA in the leading strand is synthesized in the 5’ to 3’ direction which is the ONLY wa ...
Chap3 Recombinant DNA
Chap3 Recombinant DNA

Mitochondrial DNA - MrsWrightsSciencePage
Mitochondrial DNA - MrsWrightsSciencePage

... Introns are generally NONCODING DNA segments or Junk DNA ...
Bio 6B Lecture Slides - J
Bio 6B Lecture Slides - J

... Colonies of cells containing the gene of interest have been identified by nucleic acid hybridization. Cells from colonies tagged with the probe can be grown in large tanks of liquid growth medium. Large amounts of the DNA containing the gene of interest can be isolated from these cultures. By using ...
Important advances in next generation genome editing
Important advances in next generation genome editing

... One of these tricks they can do is to serve as a sort of stop sign for the cell. When the machinery that normally reads DNA arrives at the mutant HD gene, appropriately designed genome editing tools can call them off - telling them not to do their work at that precise gene. This results in no mutant ...
Host Parasite - De Anza College
Host Parasite - De Anza College

... • Antibody dependent: target cell is coated with antibodies • NK, macrophages, neutrophils and eosinophils will bind to Fc of antibodies • Cells especially eosinophils release chemicals that lyse large pathogens ...
Timeline Review - stephen fleenor
Timeline Review - stephen fleenor

5.1.1 Cellular Control
5.1.1 Cellular Control

... can be used to trigger destruction of this RNA, preventing HIV from multiplying. Another approach is to use RNA interference to silence genes for cell surface receptors, such as the CD4 and CCR5 molecules on human white blood cells. If these genes do not produce their protein antigens, HIV cannot bi ...
Transcription and Translation Title: The Central Dogma: By Humans
Transcription and Translation Title: The Central Dogma: By Humans

... Direct the students representing mRNA to take their mRNA code through the nuclear membrane and out of the nucleus (the DNA and RNA polymerase cannot follow). Assign additional students to represent ribosomes. These students should be equipped with codon tables so that they can translate the mRNA int ...
ACQUIRED (SECONDARY) IMMUNODEFICIENCIES
ACQUIRED (SECONDARY) IMMUNODEFICIENCIES

...  New triple-drug therapy, HAART (highly active anti-retroviral therapy) ...
Molecular motors: DNA takes control
Molecular motors: DNA takes control

... before by using either proteins or DNA as a molecular scaffold3,4. These systems were primarily developed to examine the mechanisms governing collective motorforce production and transport behaviours, which are important in many transport and trafficking processes in cells. Furthermore, DNA self-ass ...
6.G Meiosis Graphic Organizer 6.H Genetic Variation
6.G Meiosis Graphic Organizer 6.H Genetic Variation

... a. creating new DNA molecules from nucleotide sequences. b. rearranging nucleotides in a gene of an organism so that new traits appear in the development of an embryo. c. moving genes from a chromosome of one organism to a chromosome of a different organism. d. building a new species by combining ge ...
12.3 DNA Replication
12.3 DNA Replication

... An enzyme called ______________ compensates for this problem by adding short, repeated DNA sequences to telomeres, _______________________ the chromosomes slightly and making it less likely that important gene sequences will be lost from the telomeres during replication. ...
Poster back - Australian Academy of Science
Poster back - Australian Academy of Science

... Diagram 4 A killer T-cell does not lock into an MHC antigen (self) without a viral antigen. But when a viral antigen binds to an MHC antigen, the shape of the MHC antigen is altered and a matching receptor on a killer T-cell can recognise the viral-MHC antigen complex (altered self). When a T-cell r ...
Slide 1
Slide 1

... Base pairs are read three at a time. Only two bases are needed to code for 16 of the 20 amino acids (4×4=16). Does this hint to an earlier, simpler chemistry? ...
Figure 1: The “Central Dogma” of Biology
Figure 1: The “Central Dogma” of Biology

... A common neurotransmitter is serotonin (which is chemically related to the amino acid tryptophan). Many widely-used antidepressants (Prozac, Zoloft, and others) inhibit the reuptake step for serotonin, and are thus called selective serotonin re-uptake inhibitors (SSRIs). They cause serotonin to accu ...
Enzyme Mechanisms - Illinois Institute of Technology
Enzyme Mechanisms - Illinois Institute of Technology

... other PTMs that eukaryotic expression enables This is considerably more complex Common approach is to use vectors derived from viruses and having the vector infect cells derived from the virus’s host Example: baculovirus, infecting lepidopteran cells; gene cloned just beyond promoter for polyhedrin, ...
Tumor Immunology and Immunotherapy
Tumor Immunology and Immunotherapy

... Gail D Sckisel, Arta M Monjazeb, Annie Mirsoian, Anthony E Zamora, Steven Grossenbacher, and William J Murphy ...
ppt
ppt

... • Complementary base-pairing with mRNA will occur  prevents translation • Use to de-activate specific mRNA’s associated with disease ...
PCR-technique Applications
PCR-technique Applications

... - group specific sequences in 16S rRNA as probes (species, ….domains) - different fluorescent dyes attached to the probe - the cells are fixated and made permeable to the probe/s - hybridization direct to the ribosomes ...
Lymphatic system - s3.amazonaws.com
Lymphatic system - s3.amazonaws.com

... cells may recognize the donor’s tissue a foreign and attempt to destroy transplanted tissue  Immunosuppresive drugs are used to reduce rejection of transplanted tissue  Interfere with recipient’s immune response by suppressing formation of antibodies or production of T cells  Leaves unprotected a ...
Summary - NIH Guidelines for Research Involving
Summary - NIH Guidelines for Research Involving

... Experiments involving recombinant DNA containing less than ½ of any eukaryotic viral genome propagated and maintained in cells in tissue culture. The use of existing tissue culture cell lines created by the introduction of recombinant or synthetic nucelic acid molecules if no further recombinant act ...
EOC Review Packet #2
EOC Review Packet #2

... • In humans there is a disease called Phenylketonuria (PKU)which is caused by a recessive allele. People with this allele have a defective enzyme and cannot break down the amino acid phenylalanine. This disease can result in mental retardation or death. Let “E” represent the normal enzyme. Also in h ...
Maxpar® Human Regulatory T Cell Phenotyping Panel Kit
Maxpar® Human Regulatory T Cell Phenotyping Panel Kit

... Description: Regulatory T cells (Tregs) are a suppressive subset of CD4+ T helper (Th) cells important for the regulation of immune responses. Tregs are defined by expression of the transcription factor Foxp3. Additional Treg markers include constitutive expression of the high-affinity IL-2Rα chain ...
Beyond Mendel: Molecular genetics, cell division, and sex
Beyond Mendel: Molecular genetics, cell division, and sex

... − the shape is largely determined by the sequence of amino acids: the protein’s primary structure − because that affects how and where the chain can bend, stick to itself, and so on − Proteins have several kinds of functions − some are structural proteins, which form the physical structure of the bo ...
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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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