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Molecular basis of the adult i phenotype and the gene responsible
Molecular basis of the adult i phenotype and the gene responsible

... decreases, until the normal Ii status of adult RBCs is reached after approximately 18 months of life.5,6 Like ABH antigens, Ii antigens are also referred to as histo-blood group antigens7 because they are detected not only on RBCs but also on the surfaces of most human cells and on soluble glycoprot ...
Analysis of the DNA microarray hybridization images using
Analysis of the DNA microarray hybridization images using

... in spots) with carefully selected and strictly specified (known) structure [4]. A single DNA array may contain many thousands of spots. Main idea of microarray experiment relies on hybridization of known DNA strands from BioChip with an unknown and fluorescently tagged nucleic acid sample in solutio ...
bacterial plasmids - Acta Medica Medianae
bacterial plasmids - Acta Medica Medianae

... Identification and classification of plasmids are based on genetic characteristics which constantly exist in all of them, as is the case with their ability to preserve themselves in a host cell, and especially regarding control of replication. However, as there are differences in the control of repl ...
Human immune responses to Plasmodium
Human immune responses to Plasmodium

Shallow Gene Pool – No Diving! The Study of Cell Reproduction
Shallow Gene Pool – No Diving! The Study of Cell Reproduction

... 1. DNA – deoxyribonucleic acid, which is the genetic material of all organisms, made up of two twisted strands of sugar-phosphate molecules and nitrogen bases. 2. gene – section of DNA on a chromosome that contains instructions for making specific proteins. 3. mutation – any permanent change in a ge ...
emboj7601802-sup
emboj7601802-sup

... the psaA 5’UTR (psaA encodes one of the major subunit of PSI, the expression of which is independent of ATP synthase biogenesis). We replaced the atpA gene from the wild-type strain by this 5’psaA-atpA chimera (fig. S2A), associated with the aadA cassette. Spectinomycin-resistant transformants aA  ...
Development of a Recombinant Protein Vaccine Based on Cell
Development of a Recombinant Protein Vaccine Based on Cell

... truncated human hepatitis B core antigen using the CFPS system. Furthermore, viral structural proteins have been successfully expressed and CFPS has been used for drug delivery and gene therapy agents [2]. Viral coat protein spontaneously encapsulates the RNA transcripts when it is folded [20]. Poly ...
Structure and Transcription of the singed Locus of Drosophila
Structure and Transcription of the singed Locus of Drosophila

Effect of a Mangosteen Dietary Supplement on Human Immune
Effect of a Mangosteen Dietary Supplement on Human Immune

... monocyte=macrophage activity, Ig deficiencies, and reduction of serum concentrations of cytokines and complement components. Moreover, it has been demonstrated that induction of oxidative stress by glutathione depletion interferes with Th1 immunity, IL-12 production, and costimulatory receptor expre ...
Current Awareness Of Issues Related To Genetically Modified Food
Current Awareness Of Issues Related To Genetically Modified Food

... because the chloroplast is enclosed in a double membrane that is a physical barrier and there are no viruses or bacteria known to infect chloroplasts that could be used as a vector for gene transfer. With biolistics, tungsten or gold particles coated with DNA are shot through the chloroplast envelop ...
(From the Variety Club Heart Hospital Pediatric Research
(From the Variety Club Heart Hospital Pediatric Research

... as lysozymes in body fluids, polyelectrolytes, complement, non-specific bactericidal substances, and phagocytosis are among the innate mechanisms of resistance. Although some of these are well developed in invertebrates and m a y indeed be the effective mechanisms of defense, they have been reviewed ...
Correlations among measles virus
Correlations among measles virus

... (r = 0·15, P = 0·005). Measles antibody levels were correlated with lymphoproliferation (r = 0·12, P = 0·03), but lacked correlation to either cytokine type. In conclusion, we demonstrated the presence of both long-term cellular and humoral responses after MMR-II vaccination in a significant proport ...
talk
talk

A Sex Chromosome Rearrangement in a Human XX
A Sex Chromosome Rearrangement in a Human XX

... et al., 1985), which consists essentially of highly homologous sequences locatedtt on both the Y chromosome short arm (Vergnaud et al., 1986) and the X chromosome long arm (Geldwerth et al., 1985). Probe 47z, which originates from the other end of the cosmid 47 insert, does not detect any Y-spettttt ...
Fluorescence In Situ Hybridization in Cardiovascular Disease
Fluorescence In Situ Hybridization in Cardiovascular Disease

Initiation of HAART during acute simian immunodeficiency virus
Initiation of HAART during acute simian immunodeficiency virus

... the CSF (1,726,000 versus 38,400 copy eq./day; slope ranges, −0.36 to −0.92 and −0.17 to −0.73, respectively). At least two factors may be responsible for the difference in the rate of decline. First, viral RNA in plasma at its peak (7 days p.i.) was 100-fold higher in plasma than in CSF. As a resul ...
Training load and URTI - ACCEPTED MS Text 01-11
Training load and URTI - ACCEPTED MS Text 01-11

... for at least 2 weeks and had not taken any medication in the 4 weeks prior to the study. All ...
Fish and Shellfish Immunology
Fish and Shellfish Immunology

... response [2,8,9]. Innate immune response mechanisms against A. hydrophila have been studied in several fish species [3,4,9]; however little is known on gilthead seabream. Understanding the immune defence mechanisms of fish against bacteria is important in terms of control and prevention, as it could p ...
Disparate developmental patterns of immune responses to bacterial
Disparate developmental patterns of immune responses to bacterial

... acid-inducible gene I (RIGI)-like receptors (RLR)9–12. In the case of virus infection, specialized PRRs that recognize viral RNA or DNA nucleic acids are remarkably conserved between teleosts and mammals, indicating an early origin of antiviral innate immunity within vertebrates13–15 (reviewed in16) ...
GENETIC VARIATION OF TASTE RECEPTORS Abstract
GENETIC VARIATION OF TASTE RECEPTORS Abstract

... Material and Methods: To determine the TAS2R38 (A262V) genotype by using the polymerase chain reaction (PCR) and restriction endonuclease digestion, Fnu4H1 enzyme. The procedure that has been done was as the following: 1. Protocol of DNA Extraction from Cheek Cell (scrape or wash): First week take a ...
Aalborg Universitet immune cells
Aalborg Universitet immune cells

... significantly increased the secretion of IL-1β, IL-6, CXCL8, IL-10, and TNF-α (p = 0.005, p = 0.019, p = 0.012, p = 0.009, and p<0.001, respectively; n = 6) from the LPS-stimulated PBMCs, as shown in Fig 1. DMSO had no effect on cytokine secretion compared to medium alone (IL-1β: p = 0.72, IL-6: p = ...
functions occur only through constant mutualism with the INTRODUCTION
functions occur only through constant mutualism with the INTRODUCTION

... stimulation of Tregs, which are the most important immune T cells aimed at suppressing immune responses to microbetriggered intestinal inflammation. For instance, it has been reported that several species of bacteria such as altered Schaedler flora resulted in a de novo expansion of mucosal Tregs in ...
PTC Receptor Project Lab Protocol
PTC Receptor Project Lab Protocol

... homozygotes. The rare AVI/AAV heterozygotes had a mean PTC score slightly, but significantly, higher than the AVI/AVI homozygotes. Finally, sequencing the PTC gene from several non-human primates determined that all were homozygous for the PAV form. Thus, the AVI nontaster haplotype arose after hum ...
Differences in Participation of Innate and Adaptive Immunity to
Differences in Participation of Innate and Adaptive Immunity to

... as described above. If the interface layer had nRBC contamination, the dextran sedimentation step and LSM density centrifugation were repeated up to 2 times until no contamination was evident. This procedure has been shown to be without effect on the MC proportions [14]. Monocytes comprised 20%– 26% ...
Do bacteria have a role in asthma development? EDITORIAL
Do bacteria have a role in asthma development? EDITORIAL

... syncytial virus (RSV) in the first years of life. Other viruses such as parainfluenza, metapneumovirus, coronavirus, adenovirus, influenza and enteroviruses have also been implicated, mostly in older patients. In ,10% of episodes multiple viruses were found [2]. Most recently, the H1N1 virus has bee ...
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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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