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Slide 1
Slide 1

... One of the most interesting discoveries of molecular biology is the nearuniversal nature of the genetic code. Although some organisms show slight variations in the amino acids assigned to particular codons, the code is always read three bases at a time and in the same direction. Despite their enormo ...
ods of time, until activated b), a activated, the I`irtrl DNA hiiacks the
ods of time, until activated b), a activated, the I`irtrl DNA hiiacks the

... co.mpenents: a nitrogenous base (in DNA, A, C, T, or G), a sugar (blue), and a phosphate group (yellow). The nucleotides are joined to one another by covalent'bonds between the sggqq of _on9 nuClgotide and the phosphate of the next. This results in a sugar-phosphate backbone, a repeating pattern of ...
Chapter 21 Nucleic Acids and Protein Synthesis
Chapter 21 Nucleic Acids and Protein Synthesis

...  Is a sequence of amino acids in a mRNA that determine the amino acid order for the protein.  Consists of sets of three bases (triplet) along the mRNA called codons.  Has a different codon for all 20 amino acids needed to build a protein.  Contains certain codons that signal the “start” and “end ...
Protein Synthesis - Workforce Solutions
Protein Synthesis - Workforce Solutions

... • This workforce solution was funded by a grant awarded under the President’s Community-Based Job Training Grants as implemented by the U.S. Department of Labor’s Employment and Training Administration. The solution was created by the grantee and does not necessarily reflect the official position of ...
Chapter 06 Lecture PowerPoint - McGraw Hill Higher Education
Chapter 06 Lecture PowerPoint - McGraw Hill Higher Education

... • Subregions 2.4 and 4.2 are involved in promoter -10 box and -35 box recognition • The s-factor by itself cannot bind to DNA, but DNA interaction with core unmasks a DNAbinding region of s • Region between amino acids 262 and 309 of b’ stimulates s binding to the nontemplate strand in the -10 regio ...
Geuvadis Analysis Meeting
Geuvadis Analysis Meeting

... - Quantified 615 datasets based on the Gencode v7 annotation - Sensitivity is a function of sequencing depth ...
Physicochemical studies on interactions between DNA and RNA
Physicochemical studies on interactions between DNA and RNA

... Figure 2. Hyperchromic changes associated with the binding of E. coli. RNA polymerase to various DNA samples as a function of temperature. The ratio of polymerase to DNA was approximately 6 enzyme molecules per thousand DNA base pairs, and was identical in all measurements. For each series of measur ...
Introduction to Nucleic Acids Definitions By definition
Introduction to Nucleic Acids Definitions By definition

... BUN’s performed in the clinical laboratory are determined by that lab’s processing instrument - many changes have occurred in the last 20 years in instrumentation. BUN’s performed in teaching, research or field/combat hospital laboratories are performed by primitive methods, relatively speaking, tha ...
Unit V DNA RNA Protein Synthesis
Unit V DNA RNA Protein Synthesis

... corresponding amino acids. Another type of RNA called transfer RNA (tRNA) is needed to bring the mRNA and amino acids together. As the code carried by mRNA is “read” on a ribosome, the proper tRNAs arrive in turn and give up the amino acids they carry to the growing polypeptide chain. The process by ...
Having it both ways: transcription factors that bind DNA and RNA
Having it both ways: transcription factors that bind DNA and RNA

... proteins that were initially categorized as DNA-binding transcription factors, but for which subsequent research has shown apparent RNA-binding activities and functions (Table 2). With the expectation that such `moonlighting' by DNA-binding proteins might be more common than previously imagined, we ...
RIBOSWITCHES - Creighton Chemistry Webserver
RIBOSWITCHES - Creighton Chemistry Webserver

... provided a wealth of information regarding RNA structure and ligand interaction ...
Ancient Ciphers: Minireview Translation in
Ancient Ciphers: Minireview Translation in

... repeats surrounding both the 16S and 23S rRNA genes. In bacteria, these repeats form helical structures within the primary transcript that are recognized and cleaved by the duplex-specific endonuclease, RNaseIII. Although RNaseIII is not an essential activity in E. coli, the alternate routes for pre ...
the function and synthesis of ribosomes
the function and synthesis of ribosomes

... of translation, and a key function of the small subunit is to discriminate against aminoacyl-tRNAs that do not match the codon on the message48. This crucial step in the decoding process was poorly understood until the demonstration that the conformation of several residues on the 16S rRNA is sensit ...
“The function and synthesis of ribosomes.” Nature Reviews Mol Cell
“The function and synthesis of ribosomes.” Nature Reviews Mol Cell

... of translation, and a key function of the small subunit is to discriminate against aminoacyl-tRNAs that do not match the codon on the message48. This crucial step in the decoding process was poorly understood until the demonstration that the conformation of several residues on the 16S rRNA is sensit ...
Translation: RNA-protein
Translation: RNA-protein

... •Genetic code –redundant but not ambiguous; no codon specifies more than one amino acid (but one amino acid may have >1 codon) ...
Microarray experiment guidelines
Microarray experiment guidelines

... genes, microarrays provide a large quantity of information about an organism/cell/tissue – whether it be mutational studies (monitoring the effects of gene expression by knocking out/in a particular gene), conditional (monitoring the effects on gene expression when presenting the organism/cell to a ...
Answer the following short questions Q 1
Answer the following short questions Q 1

... Nucleosides contain monosaccharide, D-ribose or 2-deoxy-D-ribose, linked to N-1 of pyrimidine or N-9 of purines, by a o-glycosidic linkage Each nucleotide contains nitrogen base, sugar and phosphate DNA contains the purine bases: adenine, guanine and pyrimidine bases Thymine, Cytosine RNA contains U ...
Kofi Annan - UCSF Career - University of California, San Francisco
Kofi Annan - UCSF Career - University of California, San Francisco

... Boutros-Ghali, B, Annan, K, Ki-moon, B Structure & Function Volume 1: Proteins, Eds. Thant U & Thant U, UN Press 0000 11. Name of publication here Boutros-Ghali, B, Annan, K, Ki-moon, B ...
Nucleic Acid Structures, Energetics, and Dynamics
Nucleic Acid Structures, Energetics, and Dynamics

... determined by the standard Sanger method. It may be most used in detecting small changes in sequence, such as singlebase mutations in some genetic diseases. The ultimate goal is to be able to quickly sequence any DNA or RNA from a single molecule without first amplifying it by the polymerase chain r ...
Intracellular Distribution of Radioactivity in Nucleic Acid tration of
Intracellular Distribution of Radioactivity in Nucleic Acid tration of

... tion of precursors of two different moieties of the nucleotide molecule into the nucleic acids of van ous structural and functional elements of the cell. For this purpose, @32and glycine-@-C'4 were chosen. It is now well established that nibonucleic acid (RNA) behaves in an intracellulanly heterogen ...
Genomic organization of infectious salmon anaemia virus
Genomic organization of infectious salmon anaemia virus

... Recent evidence shows that ISAV is similar to orthomyxoviruses in that it binds to sialic acid residues on host cell surfaces and undergoes fusion with the cell in acidic endosomes (Eliassen et al., 2000). The haemagglutinating and acetylesterase activities seem to be carried out by two different pr ...
Supplementary Information (doc 82K)
Supplementary Information (doc 82K)

... Table 1). The SS-cDNA was prepared using forward primers for antisense RNA and reverse primers for sense RNA against 18S rRNA (298 bp), 5.8S rRNA (262 bp) and LSU α (28S α) rRNA (266 bp). Arrow marks indicate amplified fragments obtained for respective sense and antisense RNA. -RT, cDNA was prepared ...
PDF - Andrew Rambaut
PDF - Andrew Rambaut

... bacteria and viruses have been shown to recover fitness through such compensatory changes (Burch and Chao 1999), and simulations of RNA folding have predicted widespread compensatory mutations (Wilke et al. 2003). Compensatory changes may also be caused by interactions between natural selection and ...
Codon Bingo - Flinn Scientific
Codon Bingo - Flinn Scientific

... start codon. The ribosome reads three mRNA nucleotides at a time—these base triplets are called codons. A single mRNA nucleotide sequence—adenine-uracil-guanine (AUG)—acts as the starting point for the translation of any mRNA into a chain of amino acids. There are three different codons that are rea ...
RNA gene prediction
RNA gene prediction

... distributed on the tree of life, and does not reflect the diversity accordingly ...
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RNA



Ribonucleic acid (RNA) is a polymeric molecule implicated in various biological roles in coding, decoding, regulation, and expression of genes. RNA and DNA are nucleic acids, and, along with proteins and carbohydrates, constitute the three major macromolecules essential for all known forms of life. Like DNA, RNA is assembled as a chain of nucleotides, but unlike DNA it is more often found in nature as a single-strand folded onto itself, rather than a paired double-strand. Cellular organisms use messenger RNA (mRNA) to convey genetic information (using the letters G, U, A, and C to denote the nitrogenous bases guanine, uracil, adenine, and cytosine) that directs synthesis of specific proteins. Many viruses encode their genetic information using an RNA genome.Some RNA molecules play an active role within cells by catalyzing biological reactions, controlling gene expression, or sensing and communicating responses to cellular signals. One of these active processes is protein synthesis, a universal function whereby mRNA molecules direct the assembly of proteins on ribosomes. This process uses transfer RNA (tRNA) molecules to deliver amino acids to the ribosome, where ribosomal RNA (rRNA) links amino acids together to form proteins.
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