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Two genetic codes: Repetitive syntax for active non
Two genetic codes: Repetitive syntax for active non

... RNAs remain the most ancient biological agents that connect information with meaning, in that they interact, based on their nucleic acid syntax that binds to complementary acids of the genetic alphabet. In this way, they create information bearing molecules. However, the meaning of these molecules d ...
c - Holterman
c - Holterman

Everything you wanted to know about small RNA but were afraid to ask
Everything you wanted to know about small RNA but were afraid to ask

... gene was not fully known, but RNA produced by the transgene array was a prime suspect as a mediator of silencing. Shortly thereafter, researchers studying the inconsistent ability of injected RNA to silence genes in C. elegans tested whether sense-, anti-sense, or double-stranded RNA homologous to a ...
SPRI_buffers_v2_2
SPRI_buffers_v2_2

... liquid to slide down the inside walls of the pipette to ensure an accurate volume is added. ...
The lac Operon
The lac Operon

Chapter 04
Chapter 04

... DNA contains a code that enables a cell to make RNA and proteins. Replication copies the DNA code before a cell divides. • DNA’s triplet code enables a cell to code for proteins. ...
02. Molecular basis of heredity. Realization of hereditary information
02. Molecular basis of heredity. Realization of hereditary information

... understanding the interactions between the various systems of a cell, including the interactions between DNA, RNA and protein biosynthesis and learning how these interactions are regulated. ...
B left E
B left E

... 22. Which of the following is true about post-transcriptional RNA modifications in prokaryotes A. The 5’ end of the transcript is capped and the 3’ end is polyadenylated. B. Introns are spliced out of the transcript to form the mature mRNA. C. They do not occur, since translation and trascription ar ...
Translation
Translation

... unlike messenger RNA which is transcribed by RNA polymerase II. pre-tRNAs contain introns; in bacteria these self-splice, whereas in eukaryotes and archaea they are removed by tRNA splicing endonuclease. ...
Plant and animal microRNAs: similarities and differences
Plant and animal microRNAs: similarities and differences

... endogenous target genes by cleavage or translational inhibition of their mRNA (Fig. 1a). Mutations in the gene encoding Dicer1 in Arabidopsis can have major consequences as a result of defective miRNA production (Fig. 1b). To date, miRNAs have been found in all plant and animal multicellular organis ...
genotypes
genotypes

... disease like muscular dystrophy, it is important to consider two steps. The first is to determine if the disorder is autosomal or X-linked. • If the disorder is X-linked most of the males will have the disorder because the Y-chromosome cannot mask the affects of an affected X-chromosome. A female ca ...
Polymorphic miRNA-mediated gene regulation: contribution to
Polymorphic miRNA-mediated gene regulation: contribution to

... called ‘30 compensatory’ (3C) sites. 5D sites can be subdivided in ‘seed’ sites (5DS) that base-pair exclusively with the miRNA seed, and ‘canonical’ sites (5DC) that show base-pairing in the 30 end in addition to a 7- or 8-nt seed match [23]. Whether 5D or 3C, base-pairing seems to be avoided in th ...
Document
Document

... regulation -operons, negative and positive regulation 2) Illustrate the use of genetics in understanding cellular processes 3) Cover some aspects of DNA-binding proteins ...
Regulation of Gene Expression
Regulation of Gene Expression

... and a very small fraction of the non-protein-coding DNA consists of genes for RNA such as rRNA and tRNA • A significant amount of the genome may be transcribed into noncoding RNAs (ncRNAs) • Noncoding RNAs regulate gene expression at two points: mRNA translation and chromatin configuration ...
RNA 3`-terminal phosphate cyclases and cyclase
RNA 3`-terminal phosphate cyclases and cyclase

The Family of MADS – Box Genes Controlling Flower Development
The Family of MADS – Box Genes Controlling Flower Development

... Three AP1-, Five PI-, two AP3-, two AG- and two SEP-like MADS Box genes were isolated from Crocus sativus L. The sequence alignment revealed that the five CsPI proteins contain the typical domain structure of plant MADS box proteins consisted of the conserved N-terminal MADS-box, the I domain, the c ...
A SOLUBLE RIBONUCLEIC ACID INTERMEDIATE IN PROTEIN
A SOLUBLE RIBONUCLEIC ACID INTERMEDIATE IN PROTEIN

... irreversibly into cY-peptide linkage in protein has been used in our laboratories for a number of years as a measure of protein synthesis. The essential components of this system are the microsomal ribonucleoprotein particles, certain enzymes derived from the soluble protein fraction, adenosine trip ...
幻灯片 1
幻灯片 1

... • Carry and directly transfer cancer causing genes by infecting target cells? • Be present in the germ line as provirus copies in the host DNA From infection in times past Reactivated by carcinogenic events? • Be inserted into the host cell genome and misregulate endogenous host genes? • Infect host ...
Part I. Transcription
Part I. Transcription

... Prokaryotes  lack  a  membrane-­‐bound  ________________  ,  so  transcription  and   translation  can  occur  _____________________  in  the  cytoplasm.       ...
VIRUSES
VIRUSES

... cells, and antimicrobial proteins • The body’s first lines of defense against infection are nonspecific – They do not distinguish one infectious microbe from another ...
Chapter 15: Genes and How They Work
Chapter 15: Genes and How They Work

... To find out how a eukaryotic cell uses its DNA to direct the production of particular proteins, you must first ask where in the cell the proteins are made. We can answer this question by placing cells in a medium containing radioactively labeled amino acids for a short time. The cells will take up t ...
Basic Principles of Transcription and Translation
Basic Principles of Transcription and Translation

... of the polypeptides of hemoglobin. The numbers under the RNA refer to the codons. β globin is 146 amino acids long. The β globin gene and its pre mRNA transcript have three exons corresponding to sequences that will leave the nucleus as RNA. (The 5’ UTR and 3’ UTR are parts of exons because they are ...
Protein Synthesis Paper Lab
Protein Synthesis Paper Lab

2008 LASKER AWARDS for MEDICAL RESEARCH
2008 LASKER AWARDS for MEDICAL RESEARCH

... gene—lin-14—were the antithesis of those with inactive lin-4. The animals skip early steps in development and prematurely acquire characteristics that normally appear later. These and other results suggested that lin-4 and lin-14 exert opposite effects in worm cells. To dig further into lin-14's fun ...
DNA and RNA - Mr C Biology
DNA and RNA - Mr C Biology

... Proteins are made in ribosomes in the cytoplasm. DNA cannot leave the cell nucleus. RNA is used as a messenger to carry the code to  the ribosome in the cytoplasm. The RNA must first copy the code from the DNA. This is called Transcription ­ as the code is transcribed. Once the code is copied the mR ...
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RNA silencing

RNA silencing (associated with the concept of post-transcriptional gene silencing or RNA interference) refers to a family of gene silencing effects by which the expression of one or more genes is downregulated or entirely suppressed by non-coding RNAs, particularly small RNAs. It may also refer to the introduction of a synthetic antisense RNA molecule used in scientific experiments on gene expression. RNA silencing may also be defined as sequence-specific regulation of gene expression triggered by double-stranded RNA (dsRNA). RNA silencing mechanisms are highly conserved in most eukaryotes. The most common and well-studied example is RNA interference (RNAi), in which endogenously expressed microRNA (miRNA) or exogenously derived small interfering RNA (siRNA) induces the degradation of complementary messenger RNA. Other classes of small RNA have been identified, including piwi-interacting RNA (piRNA) and its subspecies repeat associated small interfering RNA (rasiRNA).
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