Single Processing Center Models for Human Dicer and Bacterial
... function as a homodimer. Based on an X-ray structure of the Aquifex aeolicus RNase III, models of the enzyme interaction with dsRNA, and its cleavage at two composite catalytic centers, have been proposed. We have generated mutations in human Dicer and Escherichia coli RNase III residues implicated ...
... function as a homodimer. Based on an X-ray structure of the Aquifex aeolicus RNase III, models of the enzyme interaction with dsRNA, and its cleavage at two composite catalytic centers, have been proposed. We have generated mutations in human Dicer and Escherichia coli RNase III residues implicated ...
In situ hybridization
... Summary: Similar to immunohistochemistry procedure, dioxigenin (DIG) will represent the antigen. An anti-DIG antibody-AP conjugate is then attached to the DIG-labeled RNA probe. Then, an enzyme substrate will be used to detect the anti-DIG antibody. Procedure: 1. Wash in Buffer 1 at room temperature ...
... Summary: Similar to immunohistochemistry procedure, dioxigenin (DIG) will represent the antigen. An anti-DIG antibody-AP conjugate is then attached to the DIG-labeled RNA probe. Then, an enzyme substrate will be used to detect the anti-DIG antibody. Procedure: 1. Wash in Buffer 1 at room temperature ...
Nerve activates contraction
... an RNA molecule that functions as an enzyme. • Like pre-mRNA, other kinds of primary transcripts may also be spliced, but by diverse mechanisms that do not involve spliceosomes. • In a few cases, intron RNA can catalyze its own excision without proteins or extra RNA molecules. • The discovery of rib ...
... an RNA molecule that functions as an enzyme. • Like pre-mRNA, other kinds of primary transcripts may also be spliced, but by diverse mechanisms that do not involve spliceosomes. • In a few cases, intron RNA can catalyze its own excision without proteins or extra RNA molecules. • The discovery of rib ...
Motif Finding with Gibbs Sampling
... 169 transcription factors (excluding sigmas) 3322 edges 1753 activation, 1369 repression, 185 both, 3 unknown ...
... 169 transcription factors (excluding sigmas) 3322 edges 1753 activation, 1369 repression, 185 both, 3 unknown ...
Molecular cloning and functional characterisation of a glucose
... Fig. 2. Functional analysis of CaHGT1. A: Growth of AVY701. Comparison of growth of the transformant AVY701, RE700A and RE700A+vector(pYEX-BX) on 2% glucose without uracil. B: Consumption of D-glucose by AVY701, RE700A and RE700A+vector. (P) RE700A; (b) AVY701 ; (F) RE700A+vector; (7) consumption of ...
... Fig. 2. Functional analysis of CaHGT1. A: Growth of AVY701. Comparison of growth of the transformant AVY701, RE700A and RE700A+vector(pYEX-BX) on 2% glucose without uracil. B: Consumption of D-glucose by AVY701, RE700A and RE700A+vector. (P) RE700A; (b) AVY701 ; (F) RE700A+vector; (7) consumption of ...
Computational Identification of Plant MicroRNAs and
... The primary method of identifying miRNA genes has been to isolate, reverse transcribe, clone, and sequence small cellular RNAs (Lagos-Quintana et al., 2001; Lau et al., 2001; Lee and Ambros, 2001; Llave et al., 2002a; Park et al., 2002; Reinhart et al., 2002). However, molecular cloning is biased to ...
... The primary method of identifying miRNA genes has been to isolate, reverse transcribe, clone, and sequence small cellular RNAs (Lagos-Quintana et al., 2001; Lau et al., 2001; Lee and Ambros, 2001; Llave et al., 2002a; Park et al., 2002; Reinhart et al., 2002). However, molecular cloning is biased to ...
19.1 CONSTITUTIVE, INDUCIBLE AND REPRESSIBLE GENE
... This leads to diauxic pattern of growth in laboratory media containing both sugars. The Lac promoter is intrinsically "weak" because of a poor match to consensus sequence of the sigma factor. This is important for positive regulation by CAP. The mechanism by which glucose regulates adenyl cyclase ac ...
... This leads to diauxic pattern of growth in laboratory media containing both sugars. The Lac promoter is intrinsically "weak" because of a poor match to consensus sequence of the sigma factor. This is important for positive regulation by CAP. The mechanism by which glucose regulates adenyl cyclase ac ...
Chapt. 14 Eukaryotic mRNA processing I: splicing 14.1 Genes are in
... 2. Diagram the lariat mechanism of splicing. 6. Describe results of experiment showing sequence UACUAAC within yeast intron is critical for splicing 27. Describe yeast two-hybrid assay for interaction between two known proteins (ex. Fos and Jun) 28. Describe yeast two-hybrid experiment to identify u ...
... 2. Diagram the lariat mechanism of splicing. 6. Describe results of experiment showing sequence UACUAAC within yeast intron is critical for splicing 27. Describe yeast two-hybrid assay for interaction between two known proteins (ex. Fos and Jun) 28. Describe yeast two-hybrid experiment to identify u ...
lac
... • These genes are controlled. E. coli is a successful competitor in the gut because it doesn’t waste time and energy making mRNA and proteins that are not needed. The lac genes are only transcribed if lactose is present in the growth medium. ...
... • These genes are controlled. E. coli is a successful competitor in the gut because it doesn’t waste time and energy making mRNA and proteins that are not needed. The lac genes are only transcribed if lactose is present in the growth medium. ...
Cell-Free Protein Synthesis Course
... and are very valuable therapeutic drugs. Therefore, many different protein expression systems have been developed with different preferences for certain groups of proteins and/or protein applications. Besides the expression of proteins in living cells (“in vivo”), both steps of protein expression ca ...
... and are very valuable therapeutic drugs. Therefore, many different protein expression systems have been developed with different preferences for certain groups of proteins and/or protein applications. Besides the expression of proteins in living cells (“in vivo”), both steps of protein expression ca ...
Making protein (translation)
... Part II: Translation • Using the length of messenger RNA to assemble amino acids into proteins. • Takes place in a ribosome. ...
... Part II: Translation • Using the length of messenger RNA to assemble amino acids into proteins. • Takes place in a ribosome. ...
A proximal conserved repeat in the Xist gene is
... common protein-coding RNAs (Brockdorff et al., 1992; Brown et al., 1992). The Xist RNA is peculiar in that it stays in the nucleus and associates with the X chromosome, from which it is transcribed (Brown et al., 1992; Clemson et al., 1996), where it eventually induces chromosomal silencing by unkno ...
... common protein-coding RNAs (Brockdorff et al., 1992; Brown et al., 1992). The Xist RNA is peculiar in that it stays in the nucleus and associates with the X chromosome, from which it is transcribed (Brown et al., 1992; Clemson et al., 1996), where it eventually induces chromosomal silencing by unkno ...
Translation Section 1 From Genes to Proteins Chapter 10
... • Evaluate three ways that point mutations can alter genetic material. ...
... • Evaluate three ways that point mutations can alter genetic material. ...
Chapter 17 From Gene to Protein Multiple-Choice Questions
... 7) Using RNA as a template for protein synthesis instead of translating proteins directly from the DNA is advantageous for the cell because A) RNA is much more stable than DNA. B) RNA acts as an expendable copy of the genetic material. C) only one mRNA molecule can be transcribed from a single gene, ...
... 7) Using RNA as a template for protein synthesis instead of translating proteins directly from the DNA is advantageous for the cell because A) RNA is much more stable than DNA. B) RNA acts as an expendable copy of the genetic material. C) only one mRNA molecule can be transcribed from a single gene, ...
Crystal Structures of Two Viral IRES RNA Domains Bound to the
... normal growth under physiological conditions as well as responses to internal or external stresses. Viruses do not have their own translation apparatus and have to use the host’s ribosome to synthesize their viral proteins. During viral infections, host cells down-regulate capdependent initiation as ...
... normal growth under physiological conditions as well as responses to internal or external stresses. Viruses do not have their own translation apparatus and have to use the host’s ribosome to synthesize their viral proteins. During viral infections, host cells down-regulate capdependent initiation as ...
Mechanisms of Nucleolar Dominance in Animals and Plants
... were crossed, however, the D chromosomes of only one parent would form a secondary constriction in the hybrid. Heitz (32), among others, had shown that such secondary constrictions are the site at which the nucleolus forms during interphase and that constrictions only form when nucleoli are present. ...
... were crossed, however, the D chromosomes of only one parent would form a secondary constriction in the hybrid. Heitz (32), among others, had shown that such secondary constrictions are the site at which the nucleolus forms during interphase and that constrictions only form when nucleoli are present. ...
Identifying a Novel Isoform of the AZIN1 Gene by Combining High
... reading frame that would change the terminus of the subsequent protein from Ser-Asp-Glu-Asp-stop to PheArg-stop. Follow-up studies could validate this finding on the protein level and then measure gene expression of this new isoform in various tissues, subjects, and time-points. Moreover, the method ...
... reading frame that would change the terminus of the subsequent protein from Ser-Asp-Glu-Asp-stop to PheArg-stop. Follow-up studies could validate this finding on the protein level and then measure gene expression of this new isoform in various tissues, subjects, and time-points. Moreover, the method ...
Figure S1 The yellow color of the Mimulus lewisii nectar
... GUIDELESS_RNAi_F and GUIDELESS_RNAi_R (Table S1) was used to amplify the 339-bp fragment. This fragment was BLASTed against the LF10 genome assembly with an E-value cutoff of 0.1 to ensure that no other genomic regions perfectly match this fragment for a contiguous block longer than 16 bp. The final ...
... GUIDELESS_RNAi_F and GUIDELESS_RNAi_R (Table S1) was used to amplify the 339-bp fragment. This fragment was BLASTed against the LF10 genome assembly with an E-value cutoff of 0.1 to ensure that no other genomic regions perfectly match this fragment for a contiguous block longer than 16 bp. The final ...