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Bacteriophage-mediated nucleic acid immunisation
Bacteriophage-mediated nucleic acid immunisation

... 8 weeks after the primary immunisation; data not shown). This would suggest that an initial priming of the immune system against the phage carrier may be important, and speci¢c experiments to investigate the in£uence of the antiphage response on the e⁄ciency of the system are under way. Further work ...
Per cent of children with 1st cousin parents
Per cent of children with 1st cousin parents

... the fetus to detect chemical imbalances that point to specific disorders. ...
Chinese company edits pig DNA, develops piglets that will stay pet
Chinese company edits pig DNA, develops piglets that will stay pet

... pigs by changing their DNA, which contains the instructions for how each cell in the body works. DNA is passed on from parents to children. ...
Huntingtin grabs a hammer: DNA repair in HD
Huntingtin grabs a hammer: DNA repair in HD

... Truant’s team, helmed by postdoctoral researcher Tam Maiuri, used an innovative method to pursue their hypothesis, using molecules called “chromobodies.” These can attach to specific protein targets and emit fluorescent light, illuminating working proteins that can be tracked under a microscope. In ...
The case for transgenerational epigenetic inheritance in humans
The case for transgenerational epigenetic inheritance in humans

... 15, but some cases have been reported where there is no apparent mutation, but, instead, aberrant methylation, i.e., an epimutation (Buiting et al. 2003). This appears to be the result of an allele that has passed through the male germline without clearing of the silent epigenetic state previously e ...
Principles of Virology
Principles of Virology

... Key terms and questions What is an ori and what are it´s functions? What are the functions of T antigen during the SV40 infectious cycle? How are circular replicating viral genomes separated? What is a permissive and non-permissive cell/infection? A papilloma virus enters a cell and does not produce ...
Prentice Hall Biology - Mid
Prentice Hall Biology - Mid

... 2. The rows of the table should be labeled with characteristics found in both breeds of dogs. Examples might include size, color, type of coat, intelligence, aggression, and so on. Additional traits might include shape of ears, shape of muzzle (pointed or square), or length of legs with respect to b ...
Combinatorial  protein  design  by recombination in  vitro
Combinatorial protein design by recombination in vitro

... identity to cross-prime one another, they can be 'family shuffled.' Crameri et al. [22"] recently shuffled four cephalosporinase genes (57-82% identical at the DNA sequence level) to create a library of mosaic genes from which enzymes conferring high resistance to moxalactam could be isolated. An ev ...
We present here a collection of DNA sequence
We present here a collection of DNA sequence

... with the same slope as the main line. Deletions and insertions can be detected by a jagged line, and inverted repeats are shown (using a window of one nucleotide) by diagonal lines with negative slopes. The user can also use the PC function keys to select an area from the plot to either replot at hi ...
Glaciecola psychrophila sp. nov., a novel psychrophilic bacterium
Glaciecola psychrophila sp. nov., a novel psychrophilic bacterium

... The genus Glaciecola was proposed by Bowman et al. (1998) to accommodate Gram-negative, aerobic, psychrophilic, pigmented and seawater-requiring bacteria. At the time of writing, the genus comprised four recognized species: Glaciecola punicea and Glaciecola pallidula (Bowman et al., 1998), Glaciecol ...
wg: Use primers wg550F and wgABRZ with cycler profile ST
wg: Use primers wg550F and wgABRZ with cycler profile ST

... having a low e-value (<1e-50), no similar hits with low e-values that might indicate paralogs, and an amino acid sequence with no internal stop codons. The query earwig or moth sequences were constructed from RNA sequences, however, the B. sp. nr. transversale contigs were generated from genomes, th ...
DNA sequence representation by trianders and determinative
DNA sequence representation by trianders and determinative

... Abstract: A new version of DNA walks, where nucleotides are regarded unequal in their contribution to a walk is introduced, which allows us to study thoroughly the “fine structure” of nucleotide sequences. The approach is based on the assumption that nucleotides have an inner abstract characteristic ...
The Genetic Code
The Genetic Code

... shown below. The region of sequence shown is from the transcriptional start site to the transcriptional stop site. The gene’s one small intron is shown for you in bold. 5’-CTACGTACTAGCTATTCCGATCTATACTCGATCTAGTCGCATTCCGATTCGATCGTAC-3’ 3’-GATGCATGATCGATAAGGCTAGATATGAGCTAGATCAGCGTAAGGCTAAGCTAGCATG-5’ ( ...
See Fig. 13.1c
See Fig. 13.1c

... pathogenic, S. pneumoniae to live nonpathogenic cells transforming them into pathogens… It was later (1944) shown that the “transforming principle” was DNA (thus studies of transformation led to identification of DNA as the genetic material in cells). ...
cDNA chips
cDNA chips

... Genome-scale gene expression analysis ...
1. Telomeres 2. Centromeric Repeats 3. Retrotransposons (Class I
1. Telomeres 2. Centromeric Repeats 3. Retrotransposons (Class I

... In rice: Use of Activator and Ds from maize by transformation These elements can insert into a gene leading to a nonfunctional allele and phenotype Example: The promoter of frizzy panicle locus was tagged with Ds These mutations are now called “transposon-tagged” and can be cloned Example: Screen fo ...
Deep Insight Section Common fragile sites and genomic instability
Deep Insight Section Common fragile sites and genomic instability

... separated (Wei et al., 1998). Usually transcription occurs in G1 phase and sometimes in S phase. When this happens, transcription is thought to be spatially separated from replication sites (Vieira et al., 2004). Gene expression induction in mammalian cells caused recombination processes within the ...
A Variant within the DNA Repair Gene XRCC3 Is
A Variant within the DNA Repair Gene XRCC3 Is

... ing mechanism for the genetic basis of not only malignant melanoma, but of other types of cancer as well. To date there are no studies relating to how the XRCC3 polymorphism in position 18067 of exon 7 affects the overall function of the protein. The polymorphism results in an amino acid substitutio ...
Non-small-cell lung carcinoma
Non-small-cell lung carcinoma

... 1. DNA from a test sample and normal reference sample are labelled differentially, using different fluorophores, 2. hybridized to several thousand probes. The probes are derived from most of the known genes and non-coding regions of the genome, printed on a glass slide. 3. The fluorescence intensity ...
Genome sequencing, assembly and annotation
Genome sequencing, assembly and annotation

... Micro-beads are loaded onto picotiterplate and sequenced l  pyro-sequencing ...
Using a Single-Nucleotide Polymorphism to Predict
Using a Single-Nucleotide Polymorphism to Predict

... These fragment are 221 base pairs long. The "D" lane contains your digested PCR products. If the "D" lane contains a single band, your PCR products were not cut by the restriction enzyme HaeIII. In this case both copies of your TAS2R38 gene are identical (the same allele). This form of the gene has ...
Name____________________ Genetics Study Guide/Reality Check
Name____________________ Genetics Study Guide/Reality Check

... and “F2” generation mean. He first took a purebred tall and purebred short plant. All of the F1 generation was tall. Then, he took two F1 plants and crossed them together. The F2 generation had ¾ tall plants and ¼ short plants. 29. What phenotype (dominant or recessive) do all heterozygous individua ...
Duplication of Small Segments Within the Major
Duplication of Small Segments Within the Major

... indicated as boxes. Each autoradiogram panel shows lanes from the same blot probed with each of the t w o probes. Lanes 1 through 5 are Taq I,Bg/ll/BamHI. Bg/II/BspHI,Bg/II, andBg/II/Scal digested DNA from patient A in Fig 1, respectively. In the Southern assays using probe 3. germline restriction f ...
The Effects of Plasmid on Genotype and Phenotype
The Effects of Plasmid on Genotype and Phenotype

... replicate independently of the bacterial chromosome, and many plasmids can also be transferred naturally among their bacterial hosts. Generally, plasmid genes code for proteins that are not essential for the survival of their host in its normal environment. However, some plasmid DNAs encode genes th ...
Genotyping of Ryanodine receptor 1 (RYR1) gene associated with
Genotyping of Ryanodine receptor 1 (RYR1) gene associated with

... guidelines. Nanodrop (Lifetechnologies, USA) was used to examine the quantity, purity, and integrity of purified DNA templates. The purified DNA was stored at -20°C until use. PCR-RFLP and DNA sequencing Genotypes of RYR1 gene were determined by the PCR-RFLP method according to Fujii et al. (1991). ...
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Epigenomics

Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous to genomics and proteomics, which are the study of the genome and proteome of a cell (Russell 2010 p. 217 & 230). Epigenetic modifications are reversible modifications on a cell’s DNA or histones that affect gene expression without altering the DNA sequence (Russell 2010 p. 475). Two of the most characterized epigenetic modifications are DNA methylation and histone modification. Epigenetic modifications play an important role in gene expression and regulation, and are involved in numerous cellular processes such as in differentiation/development and tumorigenesis (Russell 2010 p. 597). The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high-throughput assays (Laird 2010) and.
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