Introduction to pGLO lab
... one would be able to see the green fluorescence of the GFP that is attached to the hemoglobin. It would be a bit like attaching a light bulb to the hemoglobin molecule. ...
... one would be able to see the green fluorescence of the GFP that is attached to the hemoglobin. It would be a bit like attaching a light bulb to the hemoglobin molecule. ...
DNA-dependent protein kinase in nonhomologous end joining: a
... in a manner that facilitates end joining. In contrast, the 2056 cluster (Fig. 3) has been reported to inhibit DNA end processing upon phosphorylation, suggesting that the 2609 and 2056 clusters work in an opposite direction (Cui et al., 2005). The latter finding has at present not been confirmed, an ...
... in a manner that facilitates end joining. In contrast, the 2056 cluster (Fig. 3) has been reported to inhibit DNA end processing upon phosphorylation, suggesting that the 2609 and 2056 clusters work in an opposite direction (Cui et al., 2005). The latter finding has at present not been confirmed, an ...
A genome-wide analysis of DNA methylation in buccal - VU-DARE
... remain to be elucidated 1. Interestingly, the majority of disease-associated genetic variation is located in regulatory regions of the genome 2, including transcription-factor-occupied regions and DNase I hypersensitive sites (which correspond to open chromatin) 3. This suggests that mechanisms that ...
... remain to be elucidated 1. Interestingly, the majority of disease-associated genetic variation is located in regulatory regions of the genome 2, including transcription-factor-occupied regions and DNase I hypersensitive sites (which correspond to open chromatin) 3. This suggests that mechanisms that ...
hybrid DNA molecules
... Such molecules replicate autonomously with an average copy number of 5-10 covalently closed circles per yeast cell and also replicate as a chromosomally integrated structure. This DNA may be physically isolated in intact form from either yeast or E. coli and used to transform either organism at high ...
... Such molecules replicate autonomously with an average copy number of 5-10 covalently closed circles per yeast cell and also replicate as a chromosomally integrated structure. This DNA may be physically isolated in intact form from either yeast or E. coli and used to transform either organism at high ...
Mass spectrometry and stable isotope labeling for
... to profile 80% of all rRNA methylated sites and pseudouridines present in E. coli and S. Cerevisiae. Great sensitivity and automation of the method allowed us to simultaneously monitor many rRNA modifications even in low-abundant ribosome intermediates samples, permitting quantitative analysis of mo ...
... to profile 80% of all rRNA methylated sites and pseudouridines present in E. coli and S. Cerevisiae. Great sensitivity and automation of the method allowed us to simultaneously monitor many rRNA modifications even in low-abundant ribosome intermediates samples, permitting quantitative analysis of mo ...
Unoshan_project
... directions, and the bases of the individual nucleotides are on the inside of the helix, stacked on top of each other. The larger base shape shown in the picture above depicts the purines (adenine and guanine). The other base shape in the illustration represents the pyrimidines (cytosine and thymine) ...
... directions, and the bases of the individual nucleotides are on the inside of the helix, stacked on top of each other. The larger base shape shown in the picture above depicts the purines (adenine and guanine). The other base shape in the illustration represents the pyrimidines (cytosine and thymine) ...
Mechanical opening of DNA by micromanipulation and force
... 2002 Académie des sciences/Éditions scientifiques et médicales Elsevier SAS. Tous droits réservés S 1 6 3 1 - 0 7 0 5 ( 0 2 ) 0 1 3 4 2 - 7 /FLA ...
... 2002 Académie des sciences/Éditions scientifiques et médicales Elsevier SAS. Tous droits réservés S 1 6 3 1 - 0 7 0 5 ( 0 2 ) 0 1 3 4 2 - 7 /FLA ...
Drug-specific Sites of Topoisomerase II DNA
... observed in vivo (16). Nevertheless, others groups have described differences in cleavage patterns in viral (20), episomal (21), and cellular chromatin (22) following cell treatments with structurally unrelated inhibitors, mAMSA and VM-26, suggesting that the in vivo site selectivity is determined, ...
... observed in vivo (16). Nevertheless, others groups have described differences in cleavage patterns in viral (20), episomal (21), and cellular chromatin (22) following cell treatments with structurally unrelated inhibitors, mAMSA and VM-26, suggesting that the in vivo site selectivity is determined, ...
Genetics and Genomics of Core Short Tandem Repeat Loci
... - Triallelic patterns have been observed for many of the core STR loci and recorded on the NIST STRBase Web site can occur as an imbalance in amounts between the three alleles (type 1) or equal amounts of all three alleles (type 2) ...
... - Triallelic patterns have been observed for many of the core STR loci and recorded on the NIST STRBase Web site can occur as an imbalance in amounts between the three alleles (type 1) or equal amounts of all three alleles (type 2) ...
Class VII Using cloned DNA fragments to study gene expression 1
... These differences among genes can be primarily of two types: A. Differences in the sequence of the same gene present in two different individuals or organisms. (These can be alleles or mutations) B. The “activity” of the same gene might be different in two different individuals (or tissues or organ ...
... These differences among genes can be primarily of two types: A. Differences in the sequence of the same gene present in two different individuals or organisms. (These can be alleles or mutations) B. The “activity” of the same gene might be different in two different individuals (or tissues or organ ...
Computed Cell Image Information
... of the measured nuclei and the average ploidy value of a standard population of normal cells divided by the number of measured nuclei. Boecking determined empirical thresholds for a less favorable prognosis from a study of 258 cases of histologically confirmed malignant tumors and 74 benign lesions, ...
... of the measured nuclei and the average ploidy value of a standard population of normal cells divided by the number of measured nuclei. Boecking determined empirical thresholds for a less favorable prognosis from a study of 258 cases of histologically confirmed malignant tumors and 74 benign lesions, ...
The replication of DNA
... ssDNA in a defined direction (POLARITY).This direction is define according to the strand of DNA bound. In case of a DNA helicases that functions on the lagging strand the polarity is 5’-> 3’; On the leading strand 3’-> 5’ . ...
... ssDNA in a defined direction (POLARITY).This direction is define according to the strand of DNA bound. In case of a DNA helicases that functions on the lagging strand the polarity is 5’-> 3’; On the leading strand 3’-> 5’ . ...
Soft inheritance: Challenging the Modern Synthesis
... microbial biology (especially studies of mutational mechanisms and horizontal gene transfer), from ecology (in particular ideas about niche construction and studies of extensive symbiosis), from behavior (where the transmission of information through social learning is a major focus), and from cultu ...
... microbial biology (especially studies of mutational mechanisms and horizontal gene transfer), from ecology (in particular ideas about niche construction and studies of extensive symbiosis), from behavior (where the transmission of information through social learning is a major focus), and from cultu ...
Leukaemia Section t(11;11)(q13;q23) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... control of her karyotype revealed a t(11;11)(q13;q23), in the absence of any sign of leukemia in the bone marrow, over a period of 30 months following the discover of the t(11;11). Other cases of t(11;11)(q13;q23) were: A 13-year-old girl, who have had a M4eo AML with inv(16)(p13q22). ...
... control of her karyotype revealed a t(11;11)(q13;q23), in the absence of any sign of leukemia in the bone marrow, over a period of 30 months following the discover of the t(11;11). Other cases of t(11;11)(q13;q23) were: A 13-year-old girl, who have had a M4eo AML with inv(16)(p13q22). ...
Causes and consequences of nuclear gene positioning
... (Meister et al., 2010). The positioning mechanisms appear to rely on the interaction of the inner nuclear membrane (INM) and the underlying lamina with chromatin regions dispersed throughout the linear genome, which are referred to as lamina associating domains (LADs) (Guelen et al., 2008). One mech ...
... (Meister et al., 2010). The positioning mechanisms appear to rely on the interaction of the inner nuclear membrane (INM) and the underlying lamina with chromatin regions dispersed throughout the linear genome, which are referred to as lamina associating domains (LADs) (Guelen et al., 2008). One mech ...
A one-step cloning method for the construction of somatic cell gene
... somatic cell lines is assembly of the gene targeting construct. Traditionally, this requires an amplification step to obtain two large homologous fragments of genomic DNA, followed by restriction endonuclease digestion, and then numerous cloning steps. It is an extremely time-consuming process and l ...
... somatic cell lines is assembly of the gene targeting construct. Traditionally, this requires an amplification step to obtain two large homologous fragments of genomic DNA, followed by restriction endonuclease digestion, and then numerous cloning steps. It is an extremely time-consuming process and l ...
The Recombinant DNA Controversy: A Contemporary
... the plasmid. This is important to the molecular biologist because one simply cannot see or study a single molecule of anything. Imagine studying the properties of water (or even quenching your thirst) if you had only one molecule of it. A glassful of water contains a million times a billion times a ...
... the plasmid. This is important to the molecular biologist because one simply cannot see or study a single molecule of anything. Imagine studying the properties of water (or even quenching your thirst) if you had only one molecule of it. A glassful of water contains a million times a billion times a ...
microbial genetics
... segments of definite sizes and the different enzymes differ in their pattern of cutting. Using ...
... segments of definite sizes and the different enzymes differ in their pattern of cutting. Using ...
Recombinases
... Alternating protomers within the synaptic tetramer are active at any given time. The practical consequence of this phenomenon for tyrosine recombinases is that double-strand breaks are avoided; one strand must be religated before its partner can be cleaved. ...
... Alternating protomers within the synaptic tetramer are active at any given time. The practical consequence of this phenomenon for tyrosine recombinases is that double-strand breaks are avoided; one strand must be religated before its partner can be cleaved. ...
DNA/RNA Set - MIT Edgerton Center
... the mRNA codons to an amino acid sequence. Recognize that a stop codon does not signify an amino acid. This codon causes the protein chain to fall off the ribosome. (Use Genetic Code as on p 26, 27 or use other Genetic Code Charts as the teacher selects.) 4. Predict the sequence of amino acids in t ...
... the mRNA codons to an amino acid sequence. Recognize that a stop codon does not signify an amino acid. This codon causes the protein chain to fall off the ribosome. (Use Genetic Code as on p 26, 27 or use other Genetic Code Charts as the teacher selects.) 4. Predict the sequence of amino acids in t ...
DNA/RNA Set - Edgerton Center
... the mRNA codons to an amino acid sequence. Recognize that a stop codon does not signify an amino acid. This codon causes the protein chain to fall off the ribosome. (Use Genetic Code as on p 26, 27 or use other Genetic Code Charts as the teacher selects.) 4. Predict the sequence of amino acids in t ...
... the mRNA codons to an amino acid sequence. Recognize that a stop codon does not signify an amino acid. This codon causes the protein chain to fall off the ribosome. (Use Genetic Code as on p 26, 27 or use other Genetic Code Charts as the teacher selects.) 4. Predict the sequence of amino acids in t ...
Analysis by pulsed-field gel electrophoresis mutations in the
... loci, although three of the five loci were located on the same 330-kb SmaI fragment of the wild-type strain Eagan chromosome. This fragment also contains several important virulence determinants, including the capb locus, and one of the five constitutive mutants had concomitantly lost the ability to ...
... loci, although three of the five loci were located on the same 330-kb SmaI fragment of the wild-type strain Eagan chromosome. This fragment also contains several important virulence determinants, including the capb locus, and one of the five constitutive mutants had concomitantly lost the ability to ...
Upwelling, Downwelling, and El Nino
... telomeres, have special nucleotide sequences. – In human telomeres, this sequence is typically TTAGGG, repeated between 100 and 1,000 times. Telomeres protect genes from being eroded through multiple rounds of DNA replication. ...
... telomeres, have special nucleotide sequences. – In human telomeres, this sequence is typically TTAGGG, repeated between 100 and 1,000 times. Telomeres protect genes from being eroded through multiple rounds of DNA replication. ...
Chromosomal changes associated with changes in development
... Whatever the precise mechanisms are that bring about the various rearrangements to the VSG genes, and whatever the functional requirements for the rearrangements might be, it is clear that here is a case in which defined rearrangements to the DNA which flanks a VSG gene bring about the expression of ...
... Whatever the precise mechanisms are that bring about the various rearrangements to the VSG genes, and whatever the functional requirements for the rearrangements might be, it is clear that here is a case in which defined rearrangements to the DNA which flanks a VSG gene bring about the expression of ...