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studying genomes - Laboratory of Informatics and Chemistry
studying genomes - Laboratory of Informatics and Chemistry

... exist among individuals so that they are detectable among different members in family studies. • Most variations occur within introns, have little or no effect on an organism, yet they are detectable at the DNA level and can be used as markers. ...
DNA Technology
DNA Technology

... DNA fragments can be separated through a process called gel electrophoresis.  DNA can be separated based on size and charge  The phosphate groups are negatively charged  DNA is placed in a gel and electricity is run through ...
DNA Extraction Lab - IISME Community Site
DNA Extraction Lab - IISME Community Site

... everything but that molecule. Deoxyribonucleic acid or DNA is the molecule that controls everything that happens in the cell. DNA contains the genetic code or commands that direct the activities of cells and ultimately, the body. DNA is present in all living things from bacteria to animals. In anima ...
Document
Document

... buffer (salt, pH) for enzyme to work. Mimics cellular conditions of bacteria they come from. ...
Nucleic Acid Biotechnology Techniques
Nucleic Acid Biotechnology Techniques

... growing bacterial colonies contain the plasmid of interest ...
Cloning vectors share four common properties
Cloning vectors share four common properties

... •Multiple cloning site (MCS) •Selectable marker genes •Expression vectors have sequences that allow RNA polymerase ...
Protein Synthesis SG
Protein Synthesis SG

... 19. Describe the different base substitution mutations and the results of each. 20. What is a frameshift mutation and how does it affect the outcome of the protein? 21. Is the result of a point or frameshift mutation more harmful to the organism? Explain how. 22. In what ways are mutations helpful, ...
What is a pedigree?
What is a pedigree?

... – If the disorder is dominant, one of the parents must have the disorder. – If the disorder is recessive, neither parent has to have the disorder because they can be heterozygous. ...
NUCLEUS
NUCLEUS

... Chromatine activation: Gene activity depends on: the stage of ontogenetic period, type of cell, environment. It also depends on the level of DNA condensation. Transcription is associated with nucleosomal level only. Transcriptionally active chromatin regions have core histones undergoing high rates ...
PRE-AP Stage 3 – Learning Plan
PRE-AP Stage 3 – Learning Plan

... on the family and their traits given to you. Assessment and Closing: Exit ticket will be the final product of the pedigree chart that was created. Opening: Warm-up to review Pedigrees and Karyotypes Guided Practice: Karyotype Lab-Which disorder do you have based on the karyotype. New Material: DNA f ...
Molecular Biology Unit Notes
Molecular Biology Unit Notes

... mRNA binding site P-site holds the tRNA carrying the growing polypeptide chain A-site holds tRNA to be added to the chain with a amino acid E-site is where the discharged tRNAs leave the ribosome 2. tRNA (transfer) reads Codons (made up of three consecutive nucleotides) a. tRNA transfers amio acids ...
How do you go from gene to protein?
How do you go from gene to protein?

... Each chromosome is made of many genes. Each gene is made up of a specific DNA sequence which codes for a specific amino acid sequence, otherwise called a protein. These proteins result in the presence or absence of particular traits, or phenotypes. The process of going from gene, or DNA, to protein ...
DNA Replication - Texas Tech University
DNA Replication - Texas Tech University

... transcription initiation ...
5-5-17-Cloning_Plasmids_with_Paper
5-5-17-Cloning_Plasmids_with_Paper

... These are needed to transcribe the gene properly when it is read. In addition, the HindIII & EcoR1 restriction enzyme cutting sites (sequences of bases) are marked in bold on the Jellyfish Glo gene DNA. The two restriction enzymes and their respective restriction sites are listed below. These enzyme ...
S3. Effects of Mutations on Proteins – Formative
S3. Effects of Mutations on Proteins – Formative

... 5) You have identified a previously unknown human gene that appears to have a role in autism. It is similar enough in DNA sequence to a known mouse gene that you believe that the two genes may be evolutionarily related. You determine and compare the DNA sequences, the predicted mRNA sequences, and ...
Biotechnology - Kinam Park Homepage
Biotechnology - Kinam Park Homepage

... monoclonal antibody cocktail ZMapp is a promising immune-based treatment for Ebola virus disease (EVD). A total of 72 patients were enrolled at sites in Liberia, Sierra Leone, Guinea, and the United States. Of the 71 patients who could be evaluated, 21 died, representing an overall case fatality rat ...
DNA
DNA

... • 3.1.7B Describe the use of models as an application of scientific or technological concepts. • 3.3.7C Know that every organism has a set of genetic instructions that determines inherited traits. ...
Genetics Exam 5
Genetics Exam 5

... Problems (3 points each) You want to design an oligonucleotide probe to identify a clone containing a new enzyme that you purified. You determine that the amino terminal sequence of your enzyme is: MCFYMDW What should be the sequence of the oligonucleotide probe? Indicate redundancy by putting all ...
Created with Sketch. Genetics - true or false
Created with Sketch. Genetics - true or false

... common alternative conceptions about genetics. This activity can be done individually, in pairs or as a whole class. By the end of this activity, students should be able to:  better explain some key genetics terms including ‘DNA’, ‘gene’ and ‘chromosome’  describe the basic processes by which gene ...
CHAPTERS 21 AND 22
CHAPTERS 21 AND 22

... complementary to one of the codons in mRNA Page 663 fig 21.14 Central dogma of molecular biology  The well-established process by which genetic information stored in DNA molecules is expressed in the structure of synthesized proteins There are two steps involved to flow genetic information Transcri ...
Intro to DNA and Genetics
Intro to DNA and Genetics

... A single human DNA molecule contains all the information needed for cells to create, maintain, and keep a human body alive—like an instruction manual. BUT, a cell does NOT need the entire DNA code just to do a single job, like make new hair—just the codes used to make the new hair.  DNA’s structure ...
lecture5
lecture5

... restoring the correct C. This is done without the need to break the DNA backbone (in contrast to the mechanisms of excision repair described below). Some of the drugs used in cancer chemotherapy ("chemo") also damage DNA by alkylation. Some of the methyl groups can be removed by a protein encoded by ...
Genetics Review Sheet
Genetics Review Sheet

...  What is it and why is it important? o Outline the process of protein synthesis- what are the steps that occur? o In what organelle does protein synthesis start? On what organelle are proteins actually made? o How is RNA different than DNA? o What does mRNA stand for? What does tRNA stand for? o T ...
DNA Structure and Function
DNA Structure and Function

... necessary during protein synthesis • Chromosomes – condensed form of genetic material • necessary during cell division ...
Amgen Lab 8
Amgen Lab 8

... section of (double-stranded) DNA, using existing DNA as a template for the synthesis of new DNA strands. In humans and other eukaryotes, replication occurs in the cell nucleus. ...
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Epigenomics

Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous to genomics and proteomics, which are the study of the genome and proteome of a cell (Russell 2010 p. 217 & 230). Epigenetic modifications are reversible modifications on a cell’s DNA or histones that affect gene expression without altering the DNA sequence (Russell 2010 p. 475). Two of the most characterized epigenetic modifications are DNA methylation and histone modification. Epigenetic modifications play an important role in gene expression and regulation, and are involved in numerous cellular processes such as in differentiation/development and tumorigenesis (Russell 2010 p. 597). The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high-throughput assays (Laird 2010) and.
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