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compEpiTools - Bioconductor
compEpiTools - Bioconductor

... GRanges objects, GRanges metadata, putative methylation sites and their associated absolute and relative methylation level. All these data types are highly relevant for epigenomics integrative analyses and can include but they are not limited to: base-resolution or low-resolution DNA methylation dat ...
Lab Investigation: Examining a Single Gene
Lab Investigation: Examining a Single Gene

... • Keeping in mind what a cell does when it replicates its DNA, make a list of steps involved in replicating DNA: ...
Student Guide - the BIOTECH Project
Student Guide - the BIOTECH Project

... You will load your PCR reactions and standard DNA markers sample into the gel. A standard DNA marker has a bunch of different sized pieces of DNA so you can compare it to the DNA from your PCR reaction to figure out what size piece it is. Each gel should have both positive and negative control sampl ...
Lab 6: Electrophoresis
Lab 6: Electrophoresis

... separating and identifying DNA fragments using gel electrophoresis. In this experiment, three samples of DNA from the bacteriophage lambda (48,502 base pairs in length) are separated using gel electrophoresis: one cut with the restriction endonuclease EcoRI, one cut with the restriction endonuclease ...
Your Spitting Image Guide DOC - University of Maryland School of
Your Spitting Image Guide DOC - University of Maryland School of

... 4. Once students have completed their model, discuss how genes are sections of DNA that are passed on from a parent to a child. Introduce the Human Genome Project to students. Have a class discussion about its research and why it is important to our lives. 5. Have students work in their beginning pa ...
Chapter 14 Lecture Notes: Nucleic Acids
Chapter 14 Lecture Notes: Nucleic Acids

... 19. Given the primary structure of DNA or mRNA, use the genetic code table to predict the sequence of amino acids in the polypeptide that would be produced in translation. 20. Describe the three types of RNA and understand the role of each in translation. 21. Define the term “gene expression.” 22. D ...
Chapter 29 DNA as the Genetic Material Recombination of DNA
Chapter 29 DNA as the Genetic Material Recombination of DNA

... • Messelson and Weigle showed by 13 C and 15N labeling that recombinant phage contained DNA from both “parents” ...
Nucleic acids and protein synthesis
Nucleic acids and protein synthesis

... result from the combination of nucleic acids and proteins and, in certain cells, constitute the majorJart of the solid material. ...
Exercise 5
Exercise 5

... We want to know the relationship of this class of maternal RNAs to the genes from which they are transcribed, and to the corresponding functional mRNAs from which cellular proteins are translated. At least some of this maternal RNA cannot be translated by polysomes as a message for proteins: transla ...
Histological identifications of lesions
Histological identifications of lesions

... Procedure for grossing and study of pre-neoplastic lesions: Formalin-fixed, paraffin-embedded gallbladder specimens were examined for the presence of epithelial changes – normal mucosa, hyperplasia, metaplasia, dysplasia and carcinoma in situ. The gallbladder specimen was collected from 350 patients ...
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Answer Key

... carry foreign genes along with viral DNA into the host cell clone DNA fragments cut large DNA molecules at sequence-specific sites join DNA fragments ...
Folie 1 - Indentifying Species with DNA Barcoding
Folie 1 - Indentifying Species with DNA Barcoding

... • Therefore, it is recommended to utilise several markers for the genetic identification of fishes with DNA microarrays • Nevertheless, identification of fishes is possible with DNA microarrays, but probes have to be tested intensively in ...
Transformation Lab
Transformation Lab

... They can be transferred between organisms. In the lab they can be used to manipulate and introduce DNA of interest into bacterium. ...
Cheating is so 1999
Cheating is so 1999

... and asked for help. Willard had his students scour every study they could find in which a gene was proved to influence an athletic trait. The students came up with about three dozen, including obvious qualities such as size and speed. But there were more subtle traits, too, like grip power and ...
BIO 141 PTC DNA Fingerprint Analysis
BIO 141 PTC DNA Fingerprint Analysis

... samples obtained at the crime scene, we can determine with a high degree of accuracy whether the suspect might be guilty of the crime. DNA fingerprinting is extremely useful in exonerating innocent people who are suspects in criminal cases. DNA’s structure resembles a twisted ladder called the doubl ...
PTC Assessment - Teacher Version
PTC Assessment - Teacher Version

... Answer here: The sequence from the opsin gene of P. reticulata most closely matches the distantly related species zebrafish. (only one difference in nucleotide sequence, compared to 4 differences for G. holbrookie). Therefore, the common ancestor of both guppies and zebrafish most likely had a seque ...
An Apple a Day: Extracting DNA from Any Living Thing
An Apple a Day: Extracting DNA from Any Living Thing

... 3. Examples of things with no DNA: Rocks, water, candles, plastic, and pop cans. All of these are things that never lived. B. DNA found in banana cells can be extracted using common materials. This procedure uses household equipment and store supplies to extract DNA from bananas in sufficient quant ...
Honors Genetics: Senior Exam Review Chapter 1: Introduction to
Honors Genetics: Senior Exam Review Chapter 1: Introduction to

... Be able to differentiate human chromosome numbers in -ploidy conditions and –somic conditions (Chap 6/Question 1) Remember, chromosomes are only visible during MITOSIS and MEIOSIS. Describe nondisjunction and the impact it CAN have on gamete chromosome numbers. Be able to identify the critical areas ...
Ku Binds Telomeric DNA in Vitro - Titia de Lange Lab
Ku Binds Telomeric DNA in Vitro - Titia de Lange Lab

... Recently, homologs to both Ku70 and Ku80 have been identified in budding yeast (HDF1/YKU70 and HDF2/YKU80, respectively) (18 –20). In this organism, contrary to mammalian cells, a RAD52-dependent homologous recombination pathway is responsible for the majority of the DSB repair events (21). However, ...
Chapter 6
Chapter 6

... the plasmid transfers itself. If the plasmid is integrated into the bacterial chromosome when it comes time to transfer, then it drags part or all of the bacterial genes along with it. Once inside, the new bit of DNA finds the corresponding stretch of DNA in the resident chromosome and replaces it. ...
Evolution of DNA Sequencing - Journal of the College of Physicians
Evolution of DNA Sequencing - Journal of the College of Physicians

... discrimination between fragment sizes during electrophoresis. Secondly, the analysis of highly polymorphic regions such as Major Histocompatibility Complex (MHC) which is the most polymorphic gene known,17 could generate complex data due to multiple heterozygous positions in the sequence. Thirdly, D ...
Recombinant Paper Plasmids Cut-and
Recombinant Paper Plasmids Cut-and

... sequence of the gene. There are various other methods of finding genes. After scientists have identified the gene, they must isolate it. Restriction enzymes, or endonucleases, from bacterial cells are key in this step. They are isolated from bacteria that use them to destroy, by cleaving or cutting ...
06BIO201 Exam 3 KEY
06BIO201 Exam 3 KEY

... cells that were not treated is converted into cDNA and labeled with green fluorescent nucleotides. The cDNAs are mixed together and hybridized to a microarray/ gene chip containing all the genes in the human genome (22,000), where each spot is a difference gene sequence. When you analyze the array, ...
The Difference Makers
The Difference Makers

... humans fight viruses. About 45 million to 60 million years ago, a retrovirus called MER41 invaded the genome of a primate ancestor of humans. Today, humans have hundreds of copies of the now-extinct retrovirus scattered about their genomes. Other mammals, such as lemurs, vesper bats, carnivores and ...
Visualizing Chromatin Dynamics in Cycling Cells using the
Visualizing Chromatin Dynamics in Cycling Cells using the

... photobleaching experiments. Photoactivation of paGFP-H4 in selected nuclear areas was performed and the resulting fluorescent chromatin patterns were traced through interphase and mitosis (figure 1). Researchers used photobleaching of H2B-mRFP fluorescence in combination with photoactivation of paGF ...
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Epigenomics

Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous to genomics and proteomics, which are the study of the genome and proteome of a cell (Russell 2010 p. 217 & 230). Epigenetic modifications are reversible modifications on a cell’s DNA or histones that affect gene expression without altering the DNA sequence (Russell 2010 p. 475). Two of the most characterized epigenetic modifications are DNA methylation and histone modification. Epigenetic modifications play an important role in gene expression and regulation, and are involved in numerous cellular processes such as in differentiation/development and tumorigenesis (Russell 2010 p. 597). The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high-throughput assays (Laird 2010) and.
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