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lecture-3-techniques-of-molecular-biology
lecture-3-techniques-of-molecular-biology

GUC Notes - Detailed - 23 pages - 2012-2013 - 1
GUC Notes - Detailed - 23 pages - 2012-2013 - 1

... Genome and Genes Review: I. INTRODUCTION: Genome and Genes…..September 1, 2012 A. Genome: the sum total of all the genetic information for any biologic organism 1. DNA - double strand 2. RNA - single strand 3. Expressed as the total number of nucleotides a. Human Genome: ~3,000,000,000 nucleotides ...
Slide 1
Slide 1

... • Both environmental and genetic factors play a role in the development of disease. • A genetic disorder is a disease caused by abnormalities in an individual’s genetic material. – In this course, we will consider four different types of genetic disorders: • Single-gene • Multifactorial • Chromosoma ...
August 2007
August 2007

... chromosomes. Describe how the scientific knowledge gained through the Human Genome Project presents both risk and benefit to society. ...
C15_Chan
C15_Chan

... 22 pairs of autosomes and 1 pair of sex chromosomes ...
Biology –EOC Review Sheet Science Process Skills and Lab Safety
Biology –EOC Review Sheet Science Process Skills and Lab Safety

... Three major types of RNA (Ribosomal – rRNA; Messenger – mRNA; Transfer – tRNA) Leaves the nucleus to carry out functions in cytoplasm Transcription: (mRNA is made from one strand of DNA, carries message to ribosomes) Translation: (mRNA translated into a protein at the ribosomes; tRNA transfers amino ...
Gene Section XPE (xeroderma pigmentosum, complementation group E) Atlas of Genetics and Cytogenetics
Gene Section XPE (xeroderma pigmentosum, complementation group E) Atlas of Genetics and Cytogenetics

... lesions and is inducible by treatment with DNAdamaging agents. After UV irradiation, dynamic nuclear accumulation of DDB1 from the cytoplasm was found after 24 h. The function of the gene product is not completely clarified yet. Band shift assays suggested that the XPE gene product acts as a damaged ...
DNA Fingerprinting
DNA Fingerprinting

... into the gel electrophoresis machine. In lane 1 will be DNA Sample 1 and lane 2 will be DNA Sample 2. For each sample, shade in the box beside the corresponding number of base pairs. Remember, shorter segments ...
Gene ontology and pathways
Gene ontology and pathways

... -Trends supported by several genes in a group gives more power to statistical tests vs a test for an individual gene -Need predefined groups of biologically related genes to help process our list for ...
Full Text  - J
Full Text - J

... sion of SLC39A1, which codes for the ZIP1 protein, decreased upon treatment with 40 μM Cd for 3 hr (Lee et al., 2013). Therefore, we examined the mRNA levels of SLC39A1 in HK-2 cells treated with 40 μM Cd for various time points. Indeed, the mRNA level of SLC39A1 was significantly decreased upon 3 h ...
Slide 1
Slide 1

...  The two strands are referred to as the leading strand and the lagging strand.  Replication on the leading strand is straight forward, with DNA polymerase III adding nucleotides in the 5’ 3’ direction.  On the lagging strand replication also occurs in the 5’3’direction. This results in the form ...
13.3: RNA and Gene Expression
13.3: RNA and Gene Expression

... • The relationship between genes and their effects is complex. Despite the neatness of the genetic code, every gene cannot be simply linked to a single outcome. • Some genes are expressed only at certain times or under specific conditions. • Variations and mistakes can occur at each of the steps in ...
Understanding Genetics
Understanding Genetics

... threadlike structures in the nucleus or central portion, of each human cell ...
DNA Identity
DNA Identity

... Membranes, being composed of phospholipids, can be broken easily with the use of detergents. The DNA can also be protected from effects of other cell chemicals by addition of salt (NaCl). When the salt dissociates into Na+ and Cl- ions, these charged particles prevent the negative charge of the DNA ...
TURNING PAGES
TURNING PAGES

... with DNA and with bacterial and bacteriophage mutants (and, as a concurrent sabbatical activity, learned to fly!). Then, when Fred’s Charon bacteriophages were judged to be safe enough for use in cloning human genes, our groups collaborated in isolating and characterizing the two closely related gen ...
gene therapy
gene therapy

... Gene  therapy  can  be  used  to  treat  diseases  like  cys$c  fibrosis,  sickle   cell  anemia,  and  muscular  dystrophy.   4.  How  are  viruses  used  in  gene  therapy?   Viruses  are  oYen  used  in  gene  therapy  because  they ...
code sequence practice
code sequence practice

... Transcription – making mRNA from DNA 2. If this is your original DNA strand, what is the mRNA sequence that is synthesized? DNA Strand: C A G T G C A T T mRNA strand: 3. Now go backwards, if you are given the following mRNA strand, write the DNA strand that goes with it. mRNA strand: U C G A C C G A ...
DNA & Protein Synthesis
DNA & Protein Synthesis

... CCA UUG GAU CCG ACU ...
Genetics Powerpoint
Genetics Powerpoint

... Humans have two sets of _ (hint: a number) chromosomes—one set from each parent. The specific forms of a gene that you can get are called ____________. A dominant trait is represented by a _____________ letter. Tt is an example of a ____________________ genotype. RR is an example of a ______________ ...
genetics_4
genetics_4

... experiments in their monastery garden. • In 1865 he made his work public, units of inheritance. ...
Notes on Haldane`s mapping function and physical and recomb maps
Notes on Haldane`s mapping function and physical and recomb maps

... physical map. Physical maps involve measuring the distances between genes in terms of the numbers of basepairs of DNA. These can be obtained through sequencing of the entire genome, as for many model organisms (or sequencing a portion of the genome of interest). The gene order of both maps should be ...
8 Cell Division
8 Cell Division

... 1. How does bacterial reproduction differ from eukaryotic cell reproduction? How does the bacterial genome differ in structure and size from the eukaryotic genome? Which one has nucleosomes made of histone proteins? 2. Why are chromosomes copied (replicated) before cellular division? In what phase o ...
Mathematics Semester 1 Study Guide
Mathematics Semester 1 Study Guide

... 18. Glycogen is a polysaccharide found in humans. Where is it stored and what is its function? 19. Starch is a polysaccharide found in plants. What is its function? 20. Cellulose is a polysaccharide found in plants. What is its function? 21. Oils and fats are examples of lipids. What is their chief ...
Supplementary Methods
Supplementary Methods

... (Marvel)(PBSM) for one hour at room temperature followed by an overnight incubation at 4ºC with primary antibody in PBSM. Blots were washed twice in phosphate buffered saline in 0.05% Tween-20 (PBST) at room temperature for 5 minutes each, then incubated in secondary antibody in PBST at room tempera ...
Homework Chapters 8
Homework Chapters 8

... _____ 3) If diploid cells of the fruit fly Drosophila have 10 chromosomes, how many chromosomes would a haploid Drosophila gamete have? A) one B) two C) five D) ten E) twenty _____ 4) Most genes come in alternate forms called A) chromosomes B) alleles C) loci D) gametes E) homologues _____ 5) A cell ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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