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ARTÍCULOS
ARTÍCULOS

... any posttranslational modification other than removal of the N-terminal Met residue. We constructed an H-NS–His recombinant protein and found that, as expected, it interacts with Hha. We used a Ni2ⴙ-nitrilotriacetic acid agarose method for affinity chromatography copurification of proteins to identify ...
Matthew Kwong - GEP Community Server
Matthew Kwong - GEP Community Server

... Annotation projects involving multiple species of the same genus are important for understanding genomic evolution, adaptation, and conserved function. The Drosophila 12 Genomes Consortium paper titled, "Evolution of genes and genomes on the Drosophila phylogeny" demonstrates the wealth of knowledge ...
Genetic defects causing mitochondrial respiratory
Genetic defects causing mitochondrial respiratory

... treatments will lie in a better understanding of the genetic bases of the respiratory chain defects. Special aspects of mitochondrial genetics The five enzyme complexes of the respiratory chain are each composed of multiple polypeptides, totalling over 80 different gene products. In addition, a numb ...
Reliable classification of two-class cancer data using evolutionary
Reliable classification of two-class cancer data using evolutionary

... consider the latter case and describe the procedure of filtering and normalizing the data for their use in the identification process. Since negative and very small gene expression values arise mainly due to various experimental difficulties, we first filter all expression values as follows. Any val ...
development, the Linker histone H1 is essential for Drosophila
development, the Linker histone H1 is essential for Drosophila

... 1991; Brown et al. 1996). Recent attempts to study the functions of linker histones in vivo have used gene inactivation approaches. Elimination of the linker histone-like protein Hho1p in Saccharomyces cerevisiae did not cause any major phenotypic effects, nor were any perturbations in chromosome st ...
Screening Mutant Libraries of Fungal Laccases in the Presence of
Screening Mutant Libraries of Fungal Laccases in the Presence of

... biocatalyst. An equal volume of several organic solvents was added to ABTS•+ solution, and its UV/Vis spectrum was recorded (Fig. 1). After 1 h of incubation, no dramatic change was detected for acetonitrile, ethanol, or DMSO. As it was reported elsewhere,30 dioxane decreased the stability of ABTS•+ ...
Pluripotent stem cell metabolism and mitochondria: beyond ATP
Pluripotent stem cell metabolism and mitochondria: beyond ATP

... Cellular oxygen ranges from 1.3 – 2.5%, while oxygen within mitochondria is estimated to be < 1.3% [41]. The mammalian reproductive tract, within which the preimplantation embryo develops, has been measured at 2 - 9% oxygen in the rat, rabbit, hamster and rhesus monkey [42, 43]. The uterine environm ...
Functional Genomics
Functional Genomics

... discuss what it will mean to scientists interested in the fundamentals of life. The progression of biology into a data-rich science has been orchestrated by computational biologists. On page 823, David Eisenberg and colleagues look at the role computers will play in predicting the function of a gene ...
Gene Section EXT2 (exostoses (multiple) 2) Atlas of Genetics and Cytogenetics
Gene Section EXT2 (exostoses (multiple) 2) Atlas of Genetics and Cytogenetics

... A tumour suppressor function is suggested; exostosin-2 (EXT2) is an endoplasmic reticulum localized type II transmembrane glycoprotein which together with exostosin-1 (EXT1) forms a Golgi-localized heterooligomeric complex that catalyzes heparan sulphate (HS) polymerization. It is thus hypothesized ...
Article Mitochondrial DNA turnover occurs during preimplantation
Article Mitochondrial DNA turnover occurs during preimplantation

... either be inherited, or generated de novo by adverse environmental factors that disturb mitochondrial DNA synthesis or destabilize mtDNA. The preimplantation period of development in mammals was thought to be relatively immune from environmentally induced changes to mtDNA, since no replication of mt ...
Part 3 - Bioinformatics.ca
Part 3 - Bioinformatics.ca

... thresholds • For most predictive models this ratio would increase ...
Mating-Type Genes From the Homothallic Fungus Sordaria
Mating-Type Genes From the Homothallic Fungus Sordaria

... macrospora genomic DNA and cosmid DNAs revealed that the cloned DNAs are not rearranged with respect to their true genomic organization and that the S. macrospora mating-type genes all have a single copy in the genome (data not shown). The deduced physical map of the genome in the mating-type region ...
The Mutant of sll1961, Which Encodes a Putative
The Mutant of sll1961, Which Encodes a Putative

... Many cyanobacterial mutants with different photosystem stoichiometry from that of the wild type were reported (Wilde et al., 1995, 2001; Mann et al., 2000; Shen et al., 2002; Kufryk and Vermaas, 2003; Yu et al., 2003; Wang et al., 2004). The deletion of genes encoding the subunits of PSI or PSII may ...
Genes@ Work: an efficient algorithm for pattern discovery and
Genes@ Work: an efficient algorithm for pattern discovery and

... gene (Brown et al., 2000; Eisen et al., 1998; Mateos et al., ...
Evolution of Coloration Patterns
Evolution of Coloration Patterns

... D. melanogaster (reviewed in Wittkopp et al. 2003a). Pigmentation in mice is also a vast field of research. There are more than 127 loci in mice affecting coloration (Bennett & Lamoreux 2003). Mammalian melanocytes, or pigment cells, are derived from the neural crest and migrate to various areas of t ...
simple patterns of inheritance
simple patterns of inheritance

... noticed a recurring pattern. Although there was some experimental variation, he always observed approximately a 3:1 ratio between the dominant and the recessive trait (Figure 16.5b). This quantitative observation allowed him to conclude that the two copies of a gene carried by an F1 plant segregate ...
CHK2 kinase: cancer susceptibility and cancer therapy – two sides
CHK2 kinase: cancer susceptibility and cancer therapy – two sides

... cell-cycle arrest, and evasion of senescence is a common theme in cancer48. Recent studies have implicated CHK2 as an inducer of senescence in a number of cellular contexts. CHK2 is activated by telomere erosion, which results from ongoing replication, and induces replicative senescence through p53 ...
Influenza: The Virus
Influenza: The Virus

... 8. Tilt the patient’s head back slightly (about 70o ) to straighten the passage from the front of the nose to the nasopharynx to make insertion of the swab easier 9. Gently insert the swab along the medial part of the septum, along the base of the nose, until it reaches the posterior nares – gentle ...
Multifractal characterisation of length sequences of coding and
Multifractal characterisation of length sequences of coding and

... (ii) Order all lengths of noncoding segments according to the order of noncoding segments in the complete genome. This integer sequence is named noncoding length sequence. Yu and Anh [20] proposed a time series model for the length sequences of DNA. After calculating the correlation dimensions and H ...
The molecular genetics of head development in Drosophila
The molecular genetics of head development in Drosophila

... present-day myriapods (centipedes and millipedes). The insect head, consisting of an asegmental terminal region and cephalized trunk segments, first appeared in primitive form during the Devonian period (Smart and Hughes, 1972). However, because of the scarcity of fossil material from this period, i ...
Prescribing Information
Prescribing Information

... duration will be shorter.7-9 Total body albumin is estimated to be 350 g for a 70 kg man and is distributed throughout the extracellular compartments; more than 60% is located in the extravascular fluid compartment. The half-life of albumin is 15 to 20 days with a turnover of approximately 15 g per ...
Antibiotics involved in Clostridium difficile
Antibiotics involved in Clostridium difficile

... genes encoding known, or putative, colonization factors: three adhesins, P47 (one of the two Slayer proteins), Cwp66 and Fbp68, and a protease, Cwp84. The conditions studied included hyperosmolarity, iron depletion and exposure to several antibiotics (ampicillin, clindamycin, ofloxacin, moxifloxacin ...
ABCA3 Gene Mutations in Newborns with Fatal
ABCA3 Gene Mutations in Newborns with Fatal

... gene is expressed in photoreceptors and encodes a protein that has been implicated in transporting retinal–phosphatidylethanolamine complexes in the photoreceptor membrane disks of rods.7,8 The ABCA4 gene is mutated in several recessive disorders that involve retinal degeneration, including macular ...
Molecular cloning of a rhodopsin gene from salamander rods.
Molecular cloning of a rhodopsin gene from salamander rods.

... However, the salamander opsin genes had not been cloned. The purpose of this study was to clone a salamander rhodopsin and to determine its primary structure and cell type-specific expression. Methods. Using salamander retina RNA as a template and Xenopus rhodopsin-specific oligonucleotides as prime ...
Scientific background report AMFLORA potato
Scientific background report AMFLORA potato

... to shut down production of the GBSS enzyme. Strangely enough they have introduced a genetic construct that expresses GBSS for this purpose. In other words: they have introduced an extra gbss gene, of which one would logically assume that it would rather lead to enhanced production of amylose than of ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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