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Long-span, mate-pair scaffolding and other methods for
Long-span, mate-pair scaffolding and other methods for

... be used to streamline workflow and speed up DNA library preparation for next-generation sequencing. Large insert mate-pair reads have a major impact on the overall ...
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...  The 3-base codons in the mRNA strand will pair up with anti-codons on the transfer RNA (tRNA) molecules. Each tRNA carries an amino acid to the ribosome, and these amino acids link together to form a protein. The amino acids are bound together by peptide bonds and form a polypeptide. The process i ...
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... Luc), in which a target sequence was inserted, were constructed as follows: A fragment of 35S promoter was amplified from pCAMBIA1301 by PCR using a primer set, 35S-A_PstI and 35S-B_SacI. A fragment encoding the Renilla luciferase, used as an internal standard in the SSA assay, was amplified from a ...
6.4 Traits, Genes, and Alleles
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... Question Time Distinguish between the terms locus and allele. An allele is an alternative form of a gene, which codes for a different form of the same trait. Alleles are found at the same location, or locus, on homologous chromosomes ...
14) basic genetic concepts - University of Wisconsin–Madison
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... – Clone the functional allele and insert it in a retroviral vector – Use the virus to deliver the gene to an affected cell type from the patient, such as a bone marrow cell – Viral DNA and the functional allele will insert into the patient’s chromosome – Return the cells to the patient for growth an ...
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... quantitative measurement is referred to as the transformation efficiency. In many experiments, it is important to genetically transform as many cells as possible. For example, in some types of gene therapy, cells are collected from the patient, transformed in the laboratory, and then put back into t ...
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... structural changes (meiotic recombinations) which require the formation and the repair of DSB. Deficiencies in the recombination machinery linked to exogenous agents or intrinsic causes such as gene mutations might result in ectopic synapsis and erroneous resolution of physiological DSB by non-homolo ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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