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PPT - Altogen Biosystems
PPT - Altogen Biosystems

... among a host of 100+ cell line specific In Vitro Transfection Kits. The DU145 Transfection Reagent is an advanced lipid formulation reagent, and it has been developed to provide high transfection efficiency with the DU145 cell line. This cell line is a good host for studying prostate cancers and cel ...
The nucleotides
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... Each chromosome in the nucleus of a eukaryote contains one long linear molecule of double-stranded DNA, which is bound to a complex mixture of proteins to form chromatin. Eukaryotes have also closed circular DNA molecules in their mitochondria, as do plant chloroplasts. A prokaryotic organism contai ...
Using the NCBI Genome Databases to Compare the
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... et al., 1990). In this tutorial, you will learn how to use these databases comparison is interesting because the two proteins are identical in amino and BLAST to compare human and chimpanzee hemoglobin. acid sequence but, as students will find out, the genes that code for these Hemoglobin is a prote ...
Algorithms for Genetics: Introduction, and sources of
Algorithms for Genetics: Introduction, and sources of

... species. In humans and most other animal species, the somatic cells are usually diploid, meaning they have 2 copies of each chromosome, whereas the gamete cells are haploid and have a single copy of each chromosome. Some plant and animal species are known to have more than 2 copies of each chromosom ...
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... protoplasts the next day, and I saw only sporadic nuclear division ofprotoplasts, which did not continue at all. I could not find a solution to this problem for more than six months. Then one day we decided to try another cultivar, although we felt there would not be many major differences among cul ...
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Molecules of Life Note Taking Guide

... A diet rich in saturated fats can lead to cardiovascular disease. Why do we need fats at all? Compact reservoir of energy; insulation and cushion for vital organs ...
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Tools for genetic analysis in Trypanosoma brucei unlinked fields

... other genes to compensate for the loss of the knockout target. The obvious controls are to show that each allele can be deleted independently, with either of the drug-resistance knockout constructs. The best control is to perform a conditional knockout, where the first allele is deleted and an ectop ...
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process development for monoclonal antibodies

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Fruit Fly Meiosis
Fruit Fly Meiosis

... Take 1 yellow pipe cleaner and place in cell. Flip a coin to determine if it will mutate. – Heads = mutation: Place a white bead on the pipe cleaner. ...
Local Copy - Synthetic Neurobiology Group
Local Copy - Synthetic Neurobiology Group

... in HEK293 cells (Wang et al., 2009). Of course, this method is useful for quantifying protein localization in the cells, but it does not provide information on whether the protein is properly folded and functional, within the membrane. Ultimately it is the number of proteins in the membrane that are ...
Tutorial - GeneSifter
Tutorial - GeneSifter

... mouse aortas 3. Pairwise analysis is used to identify differentially expressed genes in two groups. There are three replicates for each of the two groups in this study. Select the three replicates for the wild type mice (WT Aorta) for group 1. Select the three replicates for the apoE -/- mice (apoE ...
Biology - H Hungary is already a member of EU system so you can
Biology - H Hungary is already a member of EU system so you can

... foods contain sugars or carbohydrates ...bread, for example. Using this as an example, the sugars are broken down by enzymes that split them into the simplest form of sugar which is called glucose. Then, glucose enters the cell by special molecules in the membrane called “glucose transporters”. Once ...
Overview of DNA Purification for Nucleic Acid
Overview of DNA Purification for Nucleic Acid

... interfere with the PCR (29), or the samples can be diluted to prevent the inhibition of enzymatic reactions (30). When the amount of target material analyzed is very low, such as for the analysis of single cells or bacteria that have been concentrated by immunomagnetic separation, the DNA may actual ...
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... In many cases, diseases occur because of mutations in genes, resulting in abnormal proteins being produced by the cells of the body, or the complete absence of a protein necessary for certain functions in the cell. Mutations can occur for many different reasons, and in many different ways. For examp ...
DNA Before Proteins? Recent Discoveries in
DNA Before Proteins? Recent Discoveries in

... (1971) model where every mutation from the master sequence is considered deleterious (Kun et al., 2005; Takeuchi et al., 2005). The possibility remains, however, that the riboorganism genome had reached the maximum allowed by the fidelity of RNA polymerase ribozymes (Johnston et al., 2001; Zaher and ...
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... b) The probability of obtaining a parental chromosome (non-recombinant) in this region is the product of the probability of no recombination between A & R (70%) and the probability of no recombination between R & B (90%). This would mean that (70%)(90%) = 63% should be “parental” and 1/2 (63%) = 31. ...
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... • The number of different types of gametes can be calculated by this formula: 2n, where n is the number of homologous pairs • In a man’s testes, the number of gamete types that can be produced based on independent assortment is 223, which equals 8.5 million possibilities • If you had 23 bags, each w ...
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Diapositiva 1

... Minisatellites are molecular marker loci consisting of tandem repeat units of a 10-50 base motif, flanked by conserved endonuclease restriction sites. They are detected by gel electrophoresis of restricted DNA and subsequent Southern blot hybridization to a radiolabeled DNA probe containing multiple ...
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05 DetailLectOut 2012

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l Saccharomyces cerevisiae as a Genetic Model Organism

... carbon energy source must be added, such as glucose (dextrose), sucrose, lactic acid, or others depending on the genotype of the strain and its ability to utilize various carbon sources. Glucose is the richest and most readily available carbon source and a rich medium containing glucose is referred ...
Exceptions to Mendel`s Laws:
Exceptions to Mendel`s Laws:

...  Brown eyes might be BB or Bb… how can we tell?? ...
DNA interference: DNA-induced gene silencing in the
DNA interference: DNA-induced gene silencing in the

... invading nucleic acids, such as viruses and transposons. A host defence system was first reported in the petunia flower [1], where overexpression of mRNAs involved in floral pigmentation unexpectedly induced a reduction of such pigmentation. The gene-silencing mechanism known as RNA interference (RN ...
Pollution-fighting plants
Pollution-fighting plants

... tetracycline, it stops the GM protein from working: in effect, it acts like an antidote • So when we feed the modified mosquitoes with this supplement in the lab, they stay perfectly healthy • But when the male mosquitoes mate with females in the wild, their children inherit the lethal gene • Tetrac ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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