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Plant Molecular Biology
Plant Molecular Biology

... (or almost opposite) the protoxylem poles of the central cylinder [60, 67]. In the case of Medicago the nodule primordia are capable of further developing into full-grown nodules which have the anatomical and histological features of genuine rhizobium-induced nodules, such as apical meristems and pe ...
The role of variable DNA tandem repeats in bacterial adaptation
The role of variable DNA tandem repeats in bacterial adaptation

... to rearrange and cause frameshift mutations in genes (Coenye & Vandamme, 2005; Ackermann & Chao, 2006; Orsi et al., 2010; Lin & Kussell, 2012). In contrast, SSRs whose unit size is a multiple of three nucleotides (3, 6, 9 …) are overrepresented in open reading frames (ORFs) because their expansion o ...
Ch. 3  - SBCC Biological Sciences Department
Ch. 3 - SBCC Biological Sciences Department

... according to their positions. Membrane-spanning (transmembrane) proteins extend through the lipid bilayer and may protrude from one or both faces. Peripheral membrane proteins associate mostly with one side of the bilayer. Membrane proteins also vary in shape— they may be globular, rodlike, or fibro ...
Prenatal diagnosis of phenylketonuria
Prenatal diagnosis of phenylketonuria

... indicating that the foetus had inherited the affected allele (a) from only one parent and normal allele (b) from the other. The parents were consanguineous and hence the affected daughter was homozygous for all three loci studied. All the three markers taken together indicated that the foetus had in ...
Mcbio 316: Exam 2 ANSWER KEY (10) 1. Proteins encoded by the
Mcbio 316: Exam 2 ANSWER KEY (10) 1. Proteins encoded by the

... Note that the three phage (A1, A2, and A8) are each infecting the same pair of bacterial strains. a. Suggest an explanation for each of the six results. ANSWER: • Phage A1 cannot lyse DB21 or the P22 lysogen of DB21, indicating that it cannot adsorb to or cannot complete replication and morphogenesi ...
Nucleic acid hybridisation and polymerase chain reaction
Nucleic acid hybridisation and polymerase chain reaction

... microscopic visualisation, or by detection of biological activity or structural components (antigenic proteins and nucleic acids). The nucleotide sequence of the nucleic acid of each pathogen is different to that of other micro-organisms and susceptible hosts. This uniqueness forms the basis of two ...
Yeast epsins contain an essential N‐terminal ENTH domain, bind
Yeast epsins contain an essential N‐terminal ENTH domain, bind

... were viable and able to grow at both low and high temperatures. Proper function of the secretory and vacuolar protein sorting pathways was also confirmed (data not shown). Interestingly, however, cells in which both ENT1 and ENT2 are deleted were inviable, suggesting that these genes encode proteins ...
5-Aminoimidazole-4-Carboxamide Riboside Mimics the
5-Aminoimidazole-4-Carboxamide Riboside Mimics the

... ne of the most important actions of insulin is the regulation of gene expression. Insulin has been shown to regulate the expression of >100 genes (1). Two of the best-studied insulin-responsive genes are PEPCK (2–6) and glucose-6-phosphatase (G6Pase) (7–9). The products of these genes are key enzyme ...
Factors modifying the yield of radiation
Factors modifying the yield of radiation

... the frequencies observed in two or three FISH painted chromosomes. By PCC technique, it is possible to visualize the breaks and exchanges induced immediately and after different times following irradiation of human lymphocytes. By combining PCC with FISH it was possible to study the process of excha ...
THE G2 MICRONUCLEUS ASSAY FOR THE ANALYSIS OF IN
THE G2 MICRONUCLEUS ASSAY FOR THE ANALYSIS OF IN

... BRCA1 and BRCA2 play an important role in the DNA damage response pathway. Mutations in these genes may be associated with increased radiosensitivity, an important issue as several guidelines advise regular mammography screening from young age in BRCA1 and BRCA2 mutation carriers. To determine the d ...
Protist PowerPoint Notes
Protist PowerPoint Notes

... ___________. ___________ are short and numerous, and they move somewhat like oars on a boat. ___________ are relatively long and usually number only one or two per cell. Some flagella spin like tiny propellers, but most produce a wavelike motion from base to tip. Passive Movement Some protists are _ ...
A small organic compound enhances the religation reaction of
A small organic compound enhances the religation reaction of

... The different steps of the human Top1 (topoisomerase I) catalytic cycle have been analysed in the presence of a pentacyclic-diquinoid synthetic compound. The experiments indicate that it efficiently inhibits the cleavage step of the enzyme reaction, fitting well into the catalytic site. Surprisingly ...
Gene Regulation in Development and Evolution
Gene Regulation in Development and Evolution

... Nanog—were particularly potent in converting or reprogramming fibroblasts into iPS cells. These reprogrammed cells have most or all of the properties of bona fide ICM cells. The iPS cells can be induced to form just about any cell type, such as cardiomyocytes (heart muscle). In a further remarkable ...
Lesson Plan, GeneChip® Microarrays: Teacher`s Guide
Lesson Plan, GeneChip® Microarrays: Teacher`s Guide

... Affymetrix.com may be useful. As it is shown in the “Suggested Calendar”, the reading for the students could be assigned as homework over a one or two day time period. There are a lot of questions, though most of them are basic feedback type of questions. You may decide to cut out some of the questi ...
Phylogenetic, amino acid content and indel analyses
Phylogenetic, amino acid content and indel analyses

... rRNA for a range of bacterial species was assessed by constructing neighbour-joining trees from the β subunit data. Trees were generated using both amino acid similarities and the equivalent DNA sequence at the second codon position. Studies using the B subunit (the equivalent of the bacterial β sub ...
workshop - Dr Amy Yasko
workshop - Dr Amy Yasko

... Previous studies have suggested that lead exposure may be associated with increased risk of amyotrophic lateral sclerosis (ALS). Polymorphisms in the genes for -aminolevulinic acid dehydratase (ALAD) and the vitamin D receptor (VDR) may affect susceptibility to lead exposure. We used data from a cas ...
Mosaic screens
Mosaic screens

... 1. Direct screens: will often fail to identify essential genes required earlier in development (exceptionhypomorphic mutations). ...
Determinants of  mRNA  localization University
Determinants of mRNA localization University

... Therefore, mRNA localization may serve to increase local intracellular concentrations to promote these interactions. Conversely, the effects of non-localized protein synthesis may cause non-productive, promiscuous interactions between polypeptides, or lethal consequences in the case of morphogens. A ...
Technical file
Technical file

... Description of the insert and/or vector construction; .................. 30 d) Purity of the insert from any unknown sequence and information on the degree to which the inserted sequence is limited to the DNA required to perform the intended function;................................................. ...


... (34 oC) temperatures. We cloned the three genes by complementation (Davis 2000), utilizing the Orbach/Sachs N. crassa genomic DNA cosmid library (Orbach and Sachs 1991). The cot-2 and cot-4 genes are unlinked. However, as both genes reside on linkage group V (Perkins et al. 2001) the same complement ...
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... of such events includes the formation of γ-H2AX by phosphorylation of the serine residue 139 of histone H2AX (1–3). γ-H2AX generates a chromosomal microenvironment that facilitates recruitment of DNA repair proteins by spreading along the chromosome up to 1∼2 Mb from the damaged site. However, littl ...
Marwa Yahia Ahmed_o
Marwa Yahia Ahmed_o

... significantly higher in healthy 79-year-olds than in healthy 39-year-olds. The significance of this observation to the decline in immunity with increasing age remains to be evaluated. Interestingly, levels of IL-1Ra in amniotic fluids and urine of newborns were also shown to be significantly higher ...
Mouse Label-Retaining Cells Are Molecularly and Functionally
Mouse Label-Retaining Cells Are Molecularly and Functionally

... http://dx.doi.org/10.1053/j.gastro.2016.04.049 ...
microbial genetics
microbial genetics

... When a cell divides, the plasmid molecules are inherited by both daughter cells. For the high copy number plasmids, there are enough plasmid molecules for each daughter cell to receive at least some (Fig.4a). The daughter cells do not have to inherit exactly half of the plasmid molecules. Thus, even ...
UF study: Rapid burst of flowering plants set stage for other species
UF study: Rapid burst of flowering plants set stage for other species

... Women who reported drinking two or more sodas in the previous 24 hours were 1.86 times more likely to have albuminuria, a sensitive marker for early kidney damage. Albuminuria is an excess amount of a protein called albumin in the urine. Since healthy kidneys filter out large molecules such as album ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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