University of Groningen Live-Cell Imaging Tool Optimization To
... tion of chloramphenicol (4 mg/ml) at 30°C and shaking at 220 rpm. Fluorescence at various time points was analyzed using the Nikon TI-E microscope with a CoolSNAP H2Q camera. The fluorescein isothiocyanate (FITC) filter (excitation at 465 to 495 nm, dichroic mirror [DM] at 505 nm, barrier filter [BA ...
... tion of chloramphenicol (4 mg/ml) at 30°C and shaking at 220 rpm. Fluorescence at various time points was analyzed using the Nikon TI-E microscope with a CoolSNAP H2Q camera. The fluorescein isothiocyanate (FITC) filter (excitation at 465 to 495 nm, dichroic mirror [DM] at 505 nm, barrier filter [BA ...
Respiratory Epithelial Gene Expression in Patients with Mild and
... microarray analysis of nasal respiratory epithelium to investigate the molecular basis of phenotypic differences in CF by (1 ) identifying differences in gene expression between ⌬F508 homozygotes in the most severe 20th percentile of lung disease by forced expiratory volume in 1 s and those in the m ...
... microarray analysis of nasal respiratory epithelium to investigate the molecular basis of phenotypic differences in CF by (1 ) identifying differences in gene expression between ⌬F508 homozygotes in the most severe 20th percentile of lung disease by forced expiratory volume in 1 s and those in the m ...
GCSE Biology Textbook sample
... • The body’s cells divide and the newly formed cells are identical to the existing cells. • Cells differentiate to become specialised, and specialised cells are organised. • When cell division accelerates out of control, cancer develops. • Cells that are unspecialised in the embryo, and cells ...
... • The body’s cells divide and the newly formed cells are identical to the existing cells. • Cells differentiate to become specialised, and specialised cells are organised. • When cell division accelerates out of control, cancer develops. • Cells that are unspecialised in the embryo, and cells ...
BIO 106 Principles of Cell Biology Fall 2012 Tentative Lecture
... Concept 17.3 Eukaryotic cells modify RNA after transcription Concept 17.4 Translation is the RNA-directed synthesis of a polypeptide: a closer look Concept 17.5 Mutations of one or a few nucleotides can affect protein structure and function Concept 17.6 While gene expression differs among the domain ...
... Concept 17.3 Eukaryotic cells modify RNA after transcription Concept 17.4 Translation is the RNA-directed synthesis of a polypeptide: a closer look Concept 17.5 Mutations of one or a few nucleotides can affect protein structure and function Concept 17.6 While gene expression differs among the domain ...
Deletion of a Disease Resistance Nucleotide-Binding
... encode resistance against almost the same set of P. sojae races (Buzzell et al. 1987). We are interested in investigating the molecular differences that make the two genes distinct in their race specificities. Here we have shown that Rps4 and Rps6 are either allelic or tightly linked genes. We have ...
... encode resistance against almost the same set of P. sojae races (Buzzell et al. 1987). We are interested in investigating the molecular differences that make the two genes distinct in their race specificities. Here we have shown that Rps4 and Rps6 are either allelic or tightly linked genes. We have ...
Respiratory system 1 Prof.Faten Ghazal File
... (nose, nasal & paranasal sinuses, pharynx, and larynx) are among the most common diseases. •They are usually mild and self limiting. •They are mostly due to rhinoviruses but other types of viruses have been implicated as corona v, respiratory syncytial v, parainfluenza v, influenza v, entero v, & ad ...
... (nose, nasal & paranasal sinuses, pharynx, and larynx) are among the most common diseases. •They are usually mild and self limiting. •They are mostly due to rhinoviruses but other types of viruses have been implicated as corona v, respiratory syncytial v, parainfluenza v, influenza v, entero v, & ad ...
Isolation and characterization of Viviparous
... Arabidopsis ABI3 gene encodes a transcriptional factor homologous to maize Vp-1 (McCarty et al., 1991; Giraudat et al., 1992) and acts with ABI5 to control embryonic gene expression and seed sensitivity to ABA (Lopez-Molina et al., 2002; Nakashima et al., 2006). Mutants in the Arabidopsis ABI3 gene ...
... Arabidopsis ABI3 gene encodes a transcriptional factor homologous to maize Vp-1 (McCarty et al., 1991; Giraudat et al., 1992) and acts with ABI5 to control embryonic gene expression and seed sensitivity to ABA (Lopez-Molina et al., 2002; Nakashima et al., 2006). Mutants in the Arabidopsis ABI3 gene ...
Slide 1
... If the first T and the second –P were deleted, the relative position of T and –P would change. ...
... If the first T and the second –P were deleted, the relative position of T and –P would change. ...
NIH Public Access - University of Chicago
... transduced Ikzf1-/- progenitors generated myeloid but not B lineage progeny (data not shown, see below). We next pursued functional bypass experiments to circumvent the block in B cell development caused by the loss of Ikaros. We have previously demonstrated that the transcription factor EBF is a pr ...
... transduced Ikzf1-/- progenitors generated myeloid but not B lineage progeny (data not shown, see below). We next pursued functional bypass experiments to circumvent the block in B cell development caused by the loss of Ikaros. We have previously demonstrated that the transcription factor EBF is a pr ...
Part 2 - Laboratory of Aquaculture & Artemia Reference Center
... On going proteomic research at IART in Artemia on ...
... On going proteomic research at IART in Artemia on ...
The Genome of Deep-Sea Vent Chemolithoautotroph
... chemolithoautotrophic sulfur-oxidizing bacteria isolated from deep-sea hydrothermal vents. This gammaproteobacterium has a single chromosome (2,427,734 base pairs), and its genome illustrates many of the adaptations that have enabled it to thrive at vents globally. It has 14 methyl-accepting chemota ...
... chemolithoautotrophic sulfur-oxidizing bacteria isolated from deep-sea hydrothermal vents. This gammaproteobacterium has a single chromosome (2,427,734 base pairs), and its genome illustrates many of the adaptations that have enabled it to thrive at vents globally. It has 14 methyl-accepting chemota ...
A Primer on Gene Regulation
... the electrophoretic mobility shift assay (Figure 8). A segment of DNA containing an operator site is subjected to electrophoresis through a gel in the presence of an electric field in which the negative pole is at one end (top end in the figure) of the gel and the positive pole at the other. The DNA ...
... the electrophoretic mobility shift assay (Figure 8). A segment of DNA containing an operator site is subjected to electrophoresis through a gel in the presence of an electric field in which the negative pole is at one end (top end in the figure) of the gel and the positive pole at the other. The DNA ...
The Genetics of Beta-galactosidase
... wishes to test if the expression of a certain gene is controlled by environmental factors, a gene fusion with a promoter-less β-galactosidase can be used. This is especially useful if the gene product is uncharacterized or is not easily assayed. Along the same lines, the rate of gene expression can ...
... wishes to test if the expression of a certain gene is controlled by environmental factors, a gene fusion with a promoter-less β-galactosidase can be used. This is especially useful if the gene product is uncharacterized or is not easily assayed. Along the same lines, the rate of gene expression can ...
Genetics of male subfertility: consequences for the clinical work-up
... material are expected to occur during meiosis associated with sister chromatid exchange, spermatid development or in the spermatozoa of the germ line of the patients' fathers or de novo during early embryogenesis (Edwards and Bishop, 1997). A different mechanism leading to Y deletions could relate t ...
... material are expected to occur during meiosis associated with sister chromatid exchange, spermatid development or in the spermatozoa of the germ line of the patients' fathers or de novo during early embryogenesis (Edwards and Bishop, 1997). A different mechanism leading to Y deletions could relate t ...
GenomeSequencing_ver3_20040929
... sequence data of sufficient quality to enable gene identification but which is not sufficient to produce a finished genome sequence Paired reads: sequence reads determined from both ends of a cloned insert in a recombinant clone. Contig: contiguous DNA sequence produced from joining overlapping raw ...
... sequence data of sufficient quality to enable gene identification but which is not sufficient to produce a finished genome sequence Paired reads: sequence reads determined from both ends of a cloned insert in a recombinant clone. Contig: contiguous DNA sequence produced from joining overlapping raw ...
File
... • Errors in mitosis or meiosis can result in changes in phenotype – Changes in chromosome number often result in: ...
... • Errors in mitosis or meiosis can result in changes in phenotype – Changes in chromosome number often result in: ...
Phytozome Tutorial from David Goodstein
... • Phytozome internal identifiers (you probably won’t use this directly): transcriptid primary_transcriptid clustered proteomes ...
... • Phytozome internal identifiers (you probably won’t use this directly): transcriptid primary_transcriptid clustered proteomes ...
Genetic Markers for Sex Identification in Forensic DNA Analysis
... conserved [6,7]. The PCR products of AMELX and AMELY can be discriminated from one another using primers flanking a 6 bp deletion in the third intron of AMELX that is not present in AMELY [5,8-10]. The most commonly used amelogenin primer sets are those designed by Sullivan et al., producing AMELX/A ...
... conserved [6,7]. The PCR products of AMELX and AMELY can be discriminated from one another using primers flanking a 6 bp deletion in the third intron of AMELX that is not present in AMELY [5,8-10]. The most commonly used amelogenin primer sets are those designed by Sullivan et al., producing AMELX/A ...
Genome Analysis of Cyanobacteria
... One of the notable features of the Synechocystis genome is the presence of two types of repetitive sequences, namely, HIP1 (highly iterated palindrome) and Insertion Sequences (IS)-like elements. HIP1 is an eight-base palindromic sequence, GCGATCGC, first reported in the genomes of Synechococcus spe ...
... One of the notable features of the Synechocystis genome is the presence of two types of repetitive sequences, namely, HIP1 (highly iterated palindrome) and Insertion Sequences (IS)-like elements. HIP1 is an eight-base palindromic sequence, GCGATCGC, first reported in the genomes of Synechococcus spe ...
Regulation of biosynthesis and transport of aromatic amino acids in
... Complete genome sequences of B. subtilis, B. halodurans, Streptococcus pneumoniae, L. lactis, Streptococcus pyogenes, Clostridium acetobutylicum, Staphylococcus aureus, and Listeria monocytogenes were downloaded from GenBank (http://www.ncbi.nlm.nih.gov). Partially sequenced genomes of B. stearother ...
... Complete genome sequences of B. subtilis, B. halodurans, Streptococcus pneumoniae, L. lactis, Streptococcus pyogenes, Clostridium acetobutylicum, Staphylococcus aureus, and Listeria monocytogenes were downloaded from GenBank (http://www.ncbi.nlm.nih.gov). Partially sequenced genomes of B. stearother ...
Annotation report - GEP Community Server
... 2. For each Genscan prediction, perform a BLASTP search using the predicted amino acid sequence against the nr protein database using the strategy described above. 3. Examine the gene expression tracks (e.g., RNA-Seq) for evidence of transcribed regions that do not correspond to alignments to known ...
... 2. For each Genscan prediction, perform a BLASTP search using the predicted amino acid sequence against the nr protein database using the strategy described above. 3. Examine the gene expression tracks (e.g., RNA-Seq) for evidence of transcribed regions that do not correspond to alignments to known ...
Protein A gene expression is regulated by DNA supercoiling which
... negative supercoils into DNA by using the free energy created by ATP hydrolysis. This enzyme consists of two proteins, A and B; the active enzyme is an A2B2 complex. The A protein (GyrA) is responsible for the breakage-reunion of DNA (N-terminal domain) and for the DNA–protein interactions (C-termin ...
... negative supercoils into DNA by using the free energy created by ATP hydrolysis. This enzyme consists of two proteins, A and B; the active enzyme is an A2B2 complex. The A protein (GyrA) is responsible for the breakage-reunion of DNA (N-terminal domain) and for the DNA–protein interactions (C-termin ...