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BMC Genomics
BMC Genomics

... Genetic content of the SSRs in S. pyogenes M1 SF370 The cumulative TA-skew of strain SF370 contains five major SSRs (Fig. 1B). The nucleotide sequence of four of them corresponds to the four prophages (370.1, 370.2, 370.3 and 370.4) described in this strain [26]. The fifth SSR is a 47-kb segment con ...
Variable clinical manifestation of a novel missense mutation in the
Variable clinical manifestation of a novel missense mutation in the

... between family members. The clinical presentation of the novel mutation points towards a malignant (high incidence of sudden death) form of FHC with varying hypertrophy from mild to severe, in particular, asymmetrical septal hypertrophy. An interesting finding is the coronary abnormalities encounter ...
M-protein and other intrinsic virulence factors of Streptococcus
M-protein and other intrinsic virulence factors of Streptococcus

... Genetic content of the SSRs in S. pyogenes M1 SF370 The cumulative TA-skew of strain SF370 contains five major SSRs (Fig. 1B). The nucleotide sequence of four of them corresponds to the four prophages (370.1, 370.2, 370.3 and 370.4) described in this strain [26]. The fifth SSR is a 47-kb segment con ...
The Novel Gene HOMOLOGOUS PAIRING
The Novel Gene HOMOLOGOUS PAIRING

... although it ranged from 10% to 60%. On the other hand, no recovery was observed in all four transformants that contained the empty vector as a negative control. From these results, we concluded that this locus encoded functional the PAIR1 gene. The expression profile of PAIR1 was examined by RT-PCR ...
Significance of bacterial identification by molecular
Significance of bacterial identification by molecular

... primer sets can be used for at least three separate taxa (20). As you might expect there are some problems with this approach. The main problem is specificity for example, if a positive result occurs for a sample how do you know that it is actually an amplified product for the ...
Gene Section SIL (SCL/TAL1 interrupting locus) Atlas of Genetics and Cytogenetics
Gene Section SIL (SCL/TAL1 interrupting locus) Atlas of Genetics and Cytogenetics

... This work is licensed under a Creative Commons Attribution-Non-commercial-No Derivative Works 2.0 France Licence. © 2006 Atlas of Genetics and Cytogenetics in Oncology and Haematology ...
The Rate and Tract Length of Gene Conversion between
The Rate and Tract Length of Gene Conversion between

... of gene conversion c to the point mutation rate typically ranges from 10 to 100, which is in agreement with estimates for several duplicates in Drosophila melaogaster [49,50]. There are many locus-specific estimates of c for a wide range of species including malaria parasite [51], plant [52], avian ...
Immunology and Immunodeficiency for the Hematologist/Oncologist
Immunology and Immunodeficiency for the Hematologist/Oncologist

... Explanation: The answer is b. Presentation with Pneumocystis pneumonia is highly suggestive of T cell immunodeficiency and the profile indeed shows an absence of T and B cells. Thus this patient does not have Wiskott-Aldrich syndrome, which is characterized by low platelets, eczema and T/B cell dysf ...
The cell-cycle interactome: a source of growth
The cell-cycle interactome: a source of growth

... CYCD3;1 acts downstream of AINTEGUMENTA (ANT), a transcription factor that confers an increase in leaf size when overexpressed, due to a prolonged cell proliferation phase (Krizek, 1999; Mizukami and Fischer, 2000). When plants overexpressing CYCD3;1 are crossed to ANT-overexpressing plants, a cumul ...
Leishmania donovani - Oxford Academic
Leishmania donovani - Oxford Academic

... clamps completely around its DNA substrate. One lobe (comprising two domains resembling the core subdomains I and II of human topoisomerase I) sits on top of the DNA duplex (as oriented in Fig. 2A) just like the ‘cap’ structure referred to in the human protein (32). It is composed of mixed α and β s ...
Alternative isoleucine synthesis pathway in
Alternative isoleucine synthesis pathway in

... synthase (CimA, GSU1798) from Geobacter sulfurreducens. Reverse-transcription PCR indicated that the cce_0248 gene is expressed and its transcriptional level is lower in medium with isoleucine than in isoleucine-free medium. Additionally, a BLAST search for citramalate synthase and threonine ammonia ...
Analysis of mRNA - quantitation (contd)
Analysis of mRNA - quantitation (contd)

... – various fluorescent dye mediated methods – advantages • very fast and simple • works with tiny amounts of material – limitations • RT efficiency differs by mRNAs • Must be in linear amplification range • Errors increase exponentially with amplification BioSci D145 lecture 5 ...
Algorithm to extract REP sequences Pattern
Algorithm to extract REP sequences Pattern

... function box. It consists of the name of a function, perhaps one or more required arguments, and optional keywords and flags. A function may be thought of as a black box: you feed it information, it produces a product. ...
Vital Genes in the Heterochromatin of
Vital Genes in the Heterochromatin of

... genetic and molecular studies have shown that this ubiquitous component of eukaryotic chromosomes may perform important biological functions. In D. melanogaster, about 30 genes that are essential for viability and/or fertility have been mapped to the heterochromatin of the major autosomes. Thus far, ...
VCE Biology TSFX REVISION LECTURE UNIT 3 Part 1
VCE Biology TSFX REVISION LECTURE UNIT 3 Part 1

... that polypeptides and proteins are folded, coiled or pleated can be described by secondary (within the chain) and tertiary (overall chain shape) structures, and that those proteins made up of two or more polypeptide chains may be described by a quaternary structure. Students are expected to identify ...
Presentation: Computation to Solve Problems
Presentation: Computation to Solve Problems

... function box. It consists of the name of a function, perhaps one or more required arguments, and optional keywords and flags. A function may be thought of as a black box: you feed it information, it produces a product. ...
Biomereology_Montrea.. - Buffalo Ontology Site
Biomereology_Montrea.. - Buffalo Ontology Site

... 1) pure mathematics (theories of structures such as order, set, function, mapping) employed in every domain 2) applied mathematics, applications of these theories = re-using the same definitions, theorems, proofs in new application domains ...
Cell Cycle Control and Meiosis Notes
Cell Cycle Control and Meiosis Notes

... 8.9 Growth factors signal the cell cycle control system  Effects of a growth factor at the G1 checkpoint – A growth factor binds to a receptor in the plasma membrane – Within the cell, a signal transduction pathway propagates the signal through a series of relay molecules – The signal reaches the ...
Stochastic processes and Markov chains (part II)
Stochastic processes and Markov chains (part II)

... Processes back in time Interpretation For a reversible Markov chain it is not possible to determine the direction of the process from the observed state sequence alone. • Molecular M l l phylogenetics h l ti aims i tto reconstruct t t evolutionary relationships between present day species from thei ...
PBI 6 Features on Teacher`s Map 2-08.qxp
PBI 6 Features on Teacher`s Map 2-08.qxp

... All introns begin with the nucleotides GU and end with the nucleotides AG. These two invariant dinucleotides are not enough in themselves to define a donor splice site (at the 5' end of the intron) and an acceptor splice site (at the 3' end of the intron). The consensus donor and acceptor splice sit ...
Giant chromosomes
Giant chromosomes

... each corresponds to the loop of a sister chromatid. • The chromomere at the base of the loops consists of dense chromatin of the two sister chromatids • At the beginning of meiosis, when DNA replication is complete, the homologous pairs lie immediately next to each other and form characteristic stru ...
Ph.D. THESIS Analysis of the chromatin structure of MDR1 gene in
Ph.D. THESIS Analysis of the chromatin structure of MDR1 gene in

... caused by increased energy-dependent efflux of hydrophobic drugs that can enter the cell through diffusion. The drug efflux is generally mediated by the increased activity of a transporter, encoded by ABCB1/MDR1 (multidrug resistance 1) gene, a member of the family of ABC (ATP binding cassette) tran ...
Whole-Genome Sequence and Variant Analysis of W303, a Widely
Whole-Genome Sequence and Variant Analysis of W303, a Widely

... differences, an understanding of the precise variations at the nucleotide level between strains is an important step in elucidating the underlying causes of phenotypic differences. Since its origin, W303 has been widely used for genetic analyses of DNA repair and other biological mechanisms (THOMAS ...
Solving Genetics Problems
Solving Genetics Problems

... 2) B. If you know that they have 2 children, one that can roll and one that can not. Will that give you more info about the parent’s genotypes? r R Yes! c. Draw a punnett square: ...
Electrophoresis Systems for Nucleic Acids
Electrophoresis Systems for Nucleic Acids

... When nucleic acid (DNA/RNA) is dissolved in buffer solution, etc. it has a negative charge. When this solution (sample) is added to agarose gel and electrophoresed in buffer solution for a regulated time, the level of migration shown is in accordance with the size of nucleic acid due to the molecula ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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