Guidelines and Assignments
... 1. (MT1) A. How is the 5-mC distributed within the human genome? B. Do all human genes have CpG island at their promoters? C. How bisulfite treatment may affect the CpG methylation status? D. What methods can be used to detect the methylation status of DNA? Please describe at least four different me ...
... 1. (MT1) A. How is the 5-mC distributed within the human genome? B. Do all human genes have CpG island at their promoters? C. How bisulfite treatment may affect the CpG methylation status? D. What methods can be used to detect the methylation status of DNA? Please describe at least four different me ...
Biology Lecture 2 – Genes
... o Telomerase: lengthens telomeres (repeated sequences at ends of eukaryotic DNA, protects from being eroded) • RNA o 2’ carbon on sugar has an OH instead of an H o Generally single stranded, though can have special secondary structures o Has uracil (U) instead of T, so A=U and C≡G bonds • Types of R ...
... o Telomerase: lengthens telomeres (repeated sequences at ends of eukaryotic DNA, protects from being eroded) • RNA o 2’ carbon on sugar has an OH instead of an H o Generally single stranded, though can have special secondary structures o Has uracil (U) instead of T, so A=U and C≡G bonds • Types of R ...
Regulation of Gene Transcription
... Uses a lot of the same mechanisms as prokaryotes, but their greater complexity makes it necessary to have more complex controls as well. Eukaryotic genomes are also larger and typically have many more genes Greater regulation is also necessary to achieve finer control, this therefore increases the n ...
... Uses a lot of the same mechanisms as prokaryotes, but their greater complexity makes it necessary to have more complex controls as well. Eukaryotic genomes are also larger and typically have many more genes Greater regulation is also necessary to achieve finer control, this therefore increases the n ...
Unit VII Study Guide KEY
... _repressible____ operon, on the other hand, is normally on. Therefore, its repressor is synthesized in a _dysfunctional_______ form. An example is the _trp____ operon. When _tryptophan_____ is present in the cell due to over-production or availability from surrounding environment, it binds to the re ...
... _repressible____ operon, on the other hand, is normally on. Therefore, its repressor is synthesized in a _dysfunctional_______ form. An example is the _trp____ operon. When _tryptophan_____ is present in the cell due to over-production or availability from surrounding environment, it binds to the re ...
Microbiology: A Systems Approach, 2nd ed.
... (easier to manufacture) • For some genes, once the antisense strand bound to the mRNA, the hybrid RNA was not able to leave the nucleus • Antisense DNA: when delivered into the cytoplasm and nucleus, it binds to specific sites on any mRNAs that are the targets of therapy ...
... (easier to manufacture) • For some genes, once the antisense strand bound to the mRNA, the hybrid RNA was not able to leave the nucleus • Antisense DNA: when delivered into the cytoplasm and nucleus, it binds to specific sites on any mRNAs that are the targets of therapy ...
Positional Cloning 08
... linked to within 1 cM of a disease gene, chromosome walking can be used to clone the disease gene itself. A probe is first constructed from a genomic fragment identified from a library as being the closest linked marker to the gene. A restriction fragment isolated from the end of the clone near the ...
... linked to within 1 cM of a disease gene, chromosome walking can be used to clone the disease gene itself. A probe is first constructed from a genomic fragment identified from a library as being the closest linked marker to the gene. A restriction fragment isolated from the end of the clone near the ...
Gene Expression
... Transcription is the process of creating RNA from DNA. Transcription occurs in the cell's nucleus. RNA polymerase is the protein molecule that reads the DNA and creates the RNA intermediary. Transcription requires: DNA, RNA polymerase, ribonucleotides, and some ATP for energy. Uracil (U) is substitu ...
... Transcription is the process of creating RNA from DNA. Transcription occurs in the cell's nucleus. RNA polymerase is the protein molecule that reads the DNA and creates the RNA intermediary. Transcription requires: DNA, RNA polymerase, ribonucleotides, and some ATP for energy. Uracil (U) is substitu ...
Laboratory #1 Lecture Guide: Forensic DNA Fingerprinting
... 3. Why might it be evolutionary advantageous for bacteria to evolve to produce restriction enzymes? ...
... 3. Why might it be evolutionary advantageous for bacteria to evolve to produce restriction enzymes? ...
W09micr430Lec17 - Cal State LA
... The cell uses the DnaK-DnaJ-GrpE chaperon team to interact with σ32 at low temperature, sequestering σ32 from RNAP. Failure to bind RNAP facilitates degradation of the σ32 factor. Upon heat shock, there is an increase in the number of other unfolded or denatured proteins that can bind to DnaK or Dna ...
... The cell uses the DnaK-DnaJ-GrpE chaperon team to interact with σ32 at low temperature, sequestering σ32 from RNAP. Failure to bind RNAP facilitates degradation of the σ32 factor. Upon heat shock, there is an increase in the number of other unfolded or denatured proteins that can bind to DnaK or Dna ...
File
... DNA Fingerprinting Activity Introduction: DNA fingerprinting relies on the fact that the DNA code is universal for all living things and that there are differences between individuals within that code. Because human DNA is very similar to every other human’s DNA, DNA fingerprinting primarily focuses ...
... DNA Fingerprinting Activity Introduction: DNA fingerprinting relies on the fact that the DNA code is universal for all living things and that there are differences between individuals within that code. Because human DNA is very similar to every other human’s DNA, DNA fingerprinting primarily focuses ...
organization of chromatin and the fate of a cell
... DNA is a very long ribbon-like polymer that contains the genetic code. Even though different cell types in our body (skin cells, muscle cells, brain cells, etc.) have exactly the same DNA, these cells function very differently. How is this achieved is not well understood. We now know that the fate o ...
... DNA is a very long ribbon-like polymer that contains the genetic code. Even though different cell types in our body (skin cells, muscle cells, brain cells, etc.) have exactly the same DNA, these cells function very differently. How is this achieved is not well understood. We now know that the fate o ...
WHAT IS A GENE? II.
... In higher eukaryotes, two transcripts that originate from the same transcription start site (sharing the same promoter and regulatory elements) but do not share any sequence elements in their final products (e.g., because of alternative splicing) would not be products of the same gene. A similar log ...
... In higher eukaryotes, two transcripts that originate from the same transcription start site (sharing the same promoter and regulatory elements) but do not share any sequence elements in their final products (e.g., because of alternative splicing) would not be products of the same gene. A similar log ...
bacteriophage
... The phage fX174 is an icosahedral phage that contains a circular singlestranded DNA molecule of 5386 nucleotides. It codes for 11 proteins, each of which has been identified. Adding together the size of all those proteins comes to 2330 amino acids, which1 would require 6990 nucleotides (3 2330) – su ...
... The phage fX174 is an icosahedral phage that contains a circular singlestranded DNA molecule of 5386 nucleotides. It codes for 11 proteins, each of which has been identified. Adding together the size of all those proteins comes to 2330 amino acids, which1 would require 6990 nucleotides (3 2330) – su ...
Fruitful DNA Extraction
... studying DNA molecules). Because DNA mutations accumulate over evolutionary time, the same gene in different individuals or species may be made up of slightly different DNA sequences. This sequence of nucleotide bases is called an organism’s genotype. This molecular data can be used to determine evo ...
... studying DNA molecules). Because DNA mutations accumulate over evolutionary time, the same gene in different individuals or species may be made up of slightly different DNA sequences. This sequence of nucleotide bases is called an organism’s genotype. This molecular data can be used to determine evo ...
Applied genetics - questions
... 1 A strain of barley (A) has a high yield of seeds but a long stem which is subject to ‘lodging’ (a flattening of areas of the crop). Another strain (B) has a short, sturdy stem but a lower yield. The genotype of variety A is HHss (high yield, long stem) and the genotype of B is hhSS (low yield, sho ...
... 1 A strain of barley (A) has a high yield of seeds but a long stem which is subject to ‘lodging’ (a flattening of areas of the crop). Another strain (B) has a short, sturdy stem but a lower yield. The genotype of variety A is HHss (high yield, long stem) and the genotype of B is hhSS (low yield, sho ...
prokaryotic protein synthesis
... Bacterial DNA is found in one large, single, circular chromosome as well as several smaller loops called plasmids. The larger chromosome loop is highly condensed (supercoiled) to fit its 1100 m length into the 2 m length of the bacteria. This supercoiling also seems to be important for the DNA to ...
... Bacterial DNA is found in one large, single, circular chromosome as well as several smaller loops called plasmids. The larger chromosome loop is highly condensed (supercoiled) to fit its 1100 m length into the 2 m length of the bacteria. This supercoiling also seems to be important for the DNA to ...
Introduction to Genetics (Genetics)
... Genetics, study of the function and behavior of genes. Genes are bits of biochemical instructions found inside the cells of every organism from bacteria to humans. Offspring receive a mixture of genetic information from both parents. This process contributes to the great variation of traits that we ...
... Genetics, study of the function and behavior of genes. Genes are bits of biochemical instructions found inside the cells of every organism from bacteria to humans. Offspring receive a mixture of genetic information from both parents. This process contributes to the great variation of traits that we ...