Simple identification of dominant p53 mutants by
... are unable to function as sequence-specific transcription factors, generally because they contain mutations in the DNA binding domain which reduce the affinity for DNA (3,4). The high level of p53 protein commonly seen in tumours probably reflects the presence of a persistent p53 activating signal a ...
... are unable to function as sequence-specific transcription factors, generally because they contain mutations in the DNA binding domain which reduce the affinity for DNA (3,4). The high level of p53 protein commonly seen in tumours probably reflects the presence of a persistent p53 activating signal a ...
Oxalate decarboxylase of the white-rot fungus
... Dichomitus squalens, and cloned the corresponding Ds-odc gene. The primary structure of the Ds-ODC protein contains two conserved Mn-binding cupin motifs, but at the N-terminus, a unique, approximately 60 aa alanine-serine-rich region is found. Real-time quantitative RT-PCR analysis confirmed gene e ...
... Dichomitus squalens, and cloned the corresponding Ds-odc gene. The primary structure of the Ds-ODC protein contains two conserved Mn-binding cupin motifs, but at the N-terminus, a unique, approximately 60 aa alanine-serine-rich region is found. Real-time quantitative RT-PCR analysis confirmed gene e ...
Are You suprised ?
... is determined by the sequence of nucleotides in a gene. A change in the DNA nucleotide sequence (mutation) of a gene that codes for a protein may result in a change in the amino-acid sequence of the protein. Biochemical evidence of evolution compares favorably with structural evidence of evolution. ...
... is determined by the sequence of nucleotides in a gene. A change in the DNA nucleotide sequence (mutation) of a gene that codes for a protein may result in a change in the amino-acid sequence of the protein. Biochemical evidence of evolution compares favorably with structural evidence of evolution. ...
Identification and isolation of active N2O reducers in rice paddy soil
... Dissolved N2O is occasionally detected in surface and ground water in rice paddy fields, while little or no N2O is emitted to the atmosphere above these fields. This indicates the occurrence of N2O reduction in rice paddy fields; however, identity of the N2O reducers is largely unknown. In this stud ...
... Dissolved N2O is occasionally detected in surface and ground water in rice paddy fields, while little or no N2O is emitted to the atmosphere above these fields. This indicates the occurrence of N2O reduction in rice paddy fields; however, identity of the N2O reducers is largely unknown. In this stud ...
Evidence from glycine transfer RNA of a frozen accident at the dawn
... form. Each form could be aminoacylated with glycine by an RNA predecessor of a modern aminoacyl tRNA synthetase (Figure 1, bottom left and middle). The ligated duplex glycine RNA evolved into the modern glycine tRNA, but in this proposal has also experienced expanded evolution by duplication and mut ...
... form. Each form could be aminoacylated with glycine by an RNA predecessor of a modern aminoacyl tRNA synthetase (Figure 1, bottom left and middle). The ligated duplex glycine RNA evolved into the modern glycine tRNA, but in this proposal has also experienced expanded evolution by duplication and mut ...
VCR 221 - Potato - UC Davis Plant Sciences
... Agrobacterium transient expression system as a tool for the isolation of disease resistance genes: application to the Rx2 locus in potato. The Plant J. 21:73-81. Birhman RK and K.Hosaka 2000. Production of inbred progenies of diploid potatoes using the S locus inhibitor (Sli) gene, and their charact ...
... Agrobacterium transient expression system as a tool for the isolation of disease resistance genes: application to the Rx2 locus in potato. The Plant J. 21:73-81. Birhman RK and K.Hosaka 2000. Production of inbred progenies of diploid potatoes using the S locus inhibitor (Sli) gene, and their charact ...
The full-length HIV-1 molecular clone pLAI 61 was used to produce
... gift of Dr. Jean-Marc Jacque, University of Massachusetts Medical School, USA), and pH1sh-nef have been described previously 7,8. ...
... gift of Dr. Jean-Marc Jacque, University of Massachusetts Medical School, USA), and pH1sh-nef have been described previously 7,8. ...
Detection of GM Papaya Event 55-1 in Fresh
... the more than 130 GMOs developed in other countries have been approved in Korea [Kim et al., 2010]. Therefore, it is necessary to develop effective GMO detection methods to monitor and identify approved as well as unapproved GMOs. In this study, we designed a set of primers to amplify an endogenous ...
... the more than 130 GMOs developed in other countries have been approved in Korea [Kim et al., 2010]. Therefore, it is necessary to develop effective GMO detection methods to monitor and identify approved as well as unapproved GMOs. In this study, we designed a set of primers to amplify an endogenous ...
An Illustrated Introduction to the Basic Biological
... contrast, inert entities such as DNA have a flat landscape and generate configurations that are less biased than proteins, but lack vigorous activity and useful function except for pattern formation (Fig. 1). Compared to DNA, unstable protein biased to degraded configurations is only one of importan ...
... contrast, inert entities such as DNA have a flat landscape and generate configurations that are less biased than proteins, but lack vigorous activity and useful function except for pattern formation (Fig. 1). Compared to DNA, unstable protein biased to degraded configurations is only one of importan ...
PCR Primer Design
... PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. ...
... PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. ...
Codon usage bias from tRNA`s point of view
... species. Surprisingly, despite the wide G+C variation of bacterial genomes these anticodons are the same in most genomes. This suggests an optimization of the translation machinery to use a small subset of optimal codons and anticodons in fast-growing bacteria and in highly expressed genes. As a res ...
... species. Surprisingly, despite the wide G+C variation of bacterial genomes these anticodons are the same in most genomes. This suggests an optimization of the translation machinery to use a small subset of optimal codons and anticodons in fast-growing bacteria and in highly expressed genes. As a res ...
Genetic studies of psoriasis and psoriatic arthritis
... locus on chromosome 6, although different alleles within this locus have been found to associate with the respective diseases. While this is the strongest and most replicated locus, other susceptibility loci have also been identified through genome-wide linkage studies and candidate gene approaches. ...
... locus on chromosome 6, although different alleles within this locus have been found to associate with the respective diseases. While this is the strongest and most replicated locus, other susceptibility loci have also been identified through genome-wide linkage studies and candidate gene approaches. ...
A Novel Mouse Chromosome 17 Hybrid Sterility Locus
... are described by a notation which takes the following form: X. [Y/Z]. The first letter, outside the brackets, is indicative of the genetic background of the animal. Letters inside the brackets, separated by a "/," symbolize the chromosome I7 homologs. D represents M. domesticus, S represents M. spre ...
... are described by a notation which takes the following form: X. [Y/Z]. The first letter, outside the brackets, is indicative of the genetic background of the animal. Letters inside the brackets, separated by a "/," symbolize the chromosome I7 homologs. D represents M. domesticus, S represents M. spre ...
Chapter 4 - DORAS
... Iron nutrition minimal media bioassays were used to analyse the utilisation of haemin by the transconjugants. A 1/50 dilution of E. coli overnight cultures, which had been grown in LB broth and the appropriate antibiotics, was used to inoculate 5 ml aliquots of M63 minimal media (section 2.2). These ...
... Iron nutrition minimal media bioassays were used to analyse the utilisation of haemin by the transconjugants. A 1/50 dilution of E. coli overnight cultures, which had been grown in LB broth and the appropriate antibiotics, was used to inoculate 5 ml aliquots of M63 minimal media (section 2.2). These ...
Regulatory Motifs in DNA Sequences
... • Every gene contains a regulatory region (RR) typically stretching 100-1000 bp upstream of the transcriptional start site • Located within the RR are the Transcription Factor Binding Sites (TFBS), also known as motifs, specific for a given transcription factor • TFs influence gene expression by bin ...
... • Every gene contains a regulatory region (RR) typically stretching 100-1000 bp upstream of the transcriptional start site • Located within the RR are the Transcription Factor Binding Sites (TFBS), also known as motifs, specific for a given transcription factor • TFs influence gene expression by bin ...
Molecular mechanics of the interactions of spermine with DNA: DNA
... constants were taken from Weiner et al. (34) and Singh et al. (37), and the partial atomic charges on spermine were calculated using the Gaussian 80-UCSF program (37). Because water was not explicitly included in these calculations, we used a distancedependent dielectric constant e = R,r The structu ...
... constants were taken from Weiner et al. (34) and Singh et al. (37), and the partial atomic charges on spermine were calculated using the Gaussian 80-UCSF program (37). Because water was not explicitly included in these calculations, we used a distancedependent dielectric constant e = R,r The structu ...
Biology Final Exam Review Questions Answer Section SHORT
... In plant cells, a cell plate forms in the cytoplasm midway between each new nucleus. The cell plate gradually develops into a separating membrane, and a cell wall begins to appear in the cell plate. In animal cells, there is no cell plate. The cell membrane is drawn inward until the cytoplasm is pin ...
... In plant cells, a cell plate forms in the cytoplasm midway between each new nucleus. The cell plate gradually develops into a separating membrane, and a cell wall begins to appear in the cell plate. In animal cells, there is no cell plate. The cell membrane is drawn inward until the cytoplasm is pin ...
Notes for Chaper 4 of the Jones/Pevzer book
... “genetic” language. Symbols in “The Gold Bug” encode for a message in English • In order to solve the problem, we analyze the frequencies of patterns in DNA/Gold Bug message. • Knowledge of established regulatory motifs makes the Motif Finding problem simpler. Knowledge of the words in the English d ...
... “genetic” language. Symbols in “The Gold Bug” encode for a message in English • In order to solve the problem, we analyze the frequencies of patterns in DNA/Gold Bug message. • Knowledge of established regulatory motifs makes the Motif Finding problem simpler. Knowledge of the words in the English d ...
On the Nucleotide Sequence of Yeast Tyrosine Transfer RNA
... Figure 3 shows, however, that it is possible to construct very similar base-paired structures in spite of the limited similarities in sequences. This model was first suggested by J. R. Penswick and was further refined by E. B. Keller. Only 11 out of the 31 nucleotides shown in Table 3 as being in th ...
... Figure 3 shows, however, that it is possible to construct very similar base-paired structures in spite of the limited similarities in sequences. This model was first suggested by J. R. Penswick and was further refined by E. B. Keller. Only 11 out of the 31 nucleotides shown in Table 3 as being in th ...
Proviral amplification of the Gypsy endogenous retrovirus of
... in fertile germinal clones. Their origin can be deduced from their genotype as determined by progeny testing. The following rationale allowed us to tell which of 88 irradiated fertile females obtained in this experiment had the expected y v f mal flam1/y v f mal flam1 clone as a result of mitotic re ...
... in fertile germinal clones. Their origin can be deduced from their genotype as determined by progeny testing. The following rationale allowed us to tell which of 88 irradiated fertile females obtained in this experiment had the expected y v f mal flam1/y v f mal flam1 clone as a result of mitotic re ...
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
... have one trait in common: they employ nanoscale probes to examine the structural or electronic signatures of individual DNA bases. That is, they rely on physical differences between the bases. This is a major departure from existing sequencing paradigms which rely on chemical techniques and physical ...
... have one trait in common: they employ nanoscale probes to examine the structural or electronic signatures of individual DNA bases. That is, they rely on physical differences between the bases. This is a major departure from existing sequencing paradigms which rely on chemical techniques and physical ...
Identification of Genetic and Epigenetic Risk Factors for Psoriasis
... indicating that other genetic effects and environmental triggers are important. In order to identify novel susceptibility genes of PS and PsA, I performed the first large scale genome wide association scan for psoriasis susceptibility loci using 233 cases and 519 controls. It revealed that genes of ...
... indicating that other genetic effects and environmental triggers are important. In order to identify novel susceptibility genes of PS and PsA, I performed the first large scale genome wide association scan for psoriasis susceptibility loci using 233 cases and 519 controls. It revealed that genes of ...
Abundant RNA editing sites of chloroplast protein
... Background: RNA editing is a posttranscriptional modification process that alters the RNA sequence so that it deviates from the genomic DNA sequence. RNA editing mainly occurs in chloroplasts and mitochondrial genomes, and the number of editing sites varies in terrestrial plants. Why and how RNA edi ...
... Background: RNA editing is a posttranscriptional modification process that alters the RNA sequence so that it deviates from the genomic DNA sequence. RNA editing mainly occurs in chloroplasts and mitochondrial genomes, and the number of editing sites varies in terrestrial plants. Why and how RNA edi ...