Computational Definition of
... example. To circumvent this problem, we also compared the non-coding exons to a second class of sequences, the 5’ UTRs of intronless genes. These regions could contain the same nonsplicing information as the non-coding exons, but should lack ESEs. We compared the frequency of 8-mers (allowing one mi ...
... example. To circumvent this problem, we also compared the non-coding exons to a second class of sequences, the 5’ UTRs of intronless genes. These regions could contain the same nonsplicing information as the non-coding exons, but should lack ESEs. We compared the frequency of 8-mers (allowing one mi ...
Cells: building blocks of living organisms
... Mitochondria are energy-generating organelles (cellular power plants) ...
... Mitochondria are energy-generating organelles (cellular power plants) ...
Conservation of Brachyury (T) genes in amphioxus and vertebrates
... (Vertebrata). This contention was expanded by Beddington and Smith (1993) in a bold attempt to define a ‘molecular phylotypic stage’ for early vertebrate development, using similarities in expression patterns of several genes. Such conclusions rely on extrapolation, since mouse, Xenopus, chick and z ...
... (Vertebrata). This contention was expanded by Beddington and Smith (1993) in a bold attempt to define a ‘molecular phylotypic stage’ for early vertebrate development, using similarities in expression patterns of several genes. Such conclusions rely on extrapolation, since mouse, Xenopus, chick and z ...
BIF CH4 5th proofs.qxd
... function if one of the proteins is known only from its sequence. The operation of natural selection tends to result in the acceptance of mutations that preserve the folding and function of a protein, whereas those that destroy folding or function will be eliminated. However, similar or identical ali ...
... function if one of the proteins is known only from its sequence. The operation of natural selection tends to result in the acceptance of mutations that preserve the folding and function of a protein, whereas those that destroy folding or function will be eliminated. However, similar or identical ali ...
Zebra Finch Glucokinase Containing Two Homologous Halves Is an
... We found out that 40 amino acid residues from the Cterminus of Zebra finch glucokinase N-half actually belong to the C-terminus of hexokinase II N-half. “Original” Zebra finch glucokinase C-terminus has been reconstructed by us. It is encoded by newly mapped exon. That “new” exon has been found in c ...
... We found out that 40 amino acid residues from the Cterminus of Zebra finch glucokinase N-half actually belong to the C-terminus of hexokinase II N-half. “Original” Zebra finch glucokinase C-terminus has been reconstructed by us. It is encoded by newly mapped exon. That “new” exon has been found in c ...
The trp Operon - aandersonbiology
... 2. Explain that in this lesson they will explore Jacob-Monod’s model of gene expression, the operon. Jacob and Monod devised this model in 1961 to explain how a particular set of genes are expressed in prokaryotes. Although operons are not present in eukaryotes, the expression of genes in eukaryotes ...
... 2. Explain that in this lesson they will explore Jacob-Monod’s model of gene expression, the operon. Jacob and Monod devised this model in 1961 to explain how a particular set of genes are expressed in prokaryotes. Although operons are not present in eukaryotes, the expression of genes in eukaryotes ...
53 - Lab Times
... vectors used in cloning kits for protein expression are especially mind-boggling – at least for someone like me who has been out of the lab for a number of years. Up until the beginning of the new century, cloning of DNA was based on assembling recombinant DNA by restriction enzyme cutting and relig ...
... vectors used in cloning kits for protein expression are especially mind-boggling – at least for someone like me who has been out of the lab for a number of years. Up until the beginning of the new century, cloning of DNA was based on assembling recombinant DNA by restriction enzyme cutting and relig ...
PDF
... bp (FERRO_contig000001). The gaps between these contigs are thought to contain ribosomal RNA gene clusters since both ends of FERRO_contig000001 and one end of each of the other two contigs contain ribosomal RNA genes. The therefore nearly complete genome sequence of “Ferrovum” strain JA12 consists ...
... bp (FERRO_contig000001). The gaps between these contigs are thought to contain ribosomal RNA gene clusters since both ends of FERRO_contig000001 and one end of each of the other two contigs contain ribosomal RNA genes. The therefore nearly complete genome sequence of “Ferrovum” strain JA12 consists ...
Fibrinogen Bern I: Substitution y 337 Asn + Lys Is
... at 37°C with 0.3 U/mL plasminogen (Kabi) and 6 U/mL streptokinase (Kabi). The resulting fragmentsD,, D,, and D, were reduced with 2% dithiothreitol (Fluka) and separated by isoelectric focusing and sodium dodecyl sulfate (SDS) polyacrylamidegel electrophoresis according to Reber et aLzo DNA isolatio ...
... at 37°C with 0.3 U/mL plasminogen (Kabi) and 6 U/mL streptokinase (Kabi). The resulting fragmentsD,, D,, and D, were reduced with 2% dithiothreitol (Fluka) and separated by isoelectric focusing and sodium dodecyl sulfate (SDS) polyacrylamidegel electrophoresis according to Reber et aLzo DNA isolatio ...
Tutorial - QIAGEN Bioinformatics
... Bisulfite sequencing is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. Changes in cytosine methylation levels are implicated in regulation of gene expression, and have been shown ...
... Bisulfite sequencing is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. Changes in cytosine methylation levels are implicated in regulation of gene expression, and have been shown ...
Interrogating the Function of Metazoan Histones using
... diversity of cells. Each cell type differentially regulates genome activity to accurately execute a particular program of gene expression, cell-cycle progression, and DNA replication. Failure of this execution can lead to developmental defects or disease states that reduce organismal fitness. Becaus ...
... diversity of cells. Each cell type differentially regulates genome activity to accurately execute a particular program of gene expression, cell-cycle progression, and DNA replication. Failure of this execution can lead to developmental defects or disease states that reduce organismal fitness. Becaus ...
NIH Public Access
... which is the model acetogenic bacterium that has been widely used for elucidating the WoodLjungdahl pathway of CO and CO2 fixation. This pathway, which is also known as the reductive acetyl-CoA pathway, allows acetogenic (often called homoacetogenic) bacteria to convert glucose stoichiometrically in ...
... which is the model acetogenic bacterium that has been widely used for elucidating the WoodLjungdahl pathway of CO and CO2 fixation. This pathway, which is also known as the reductive acetyl-CoA pathway, allows acetogenic (often called homoacetogenic) bacteria to convert glucose stoichiometrically in ...
Classification of Centers for Disease Control Group Eugonic
... In addition, both biotypes can be distinguished based on DNA G+C content (Bercovier et al., 1982), cellular fatty acid content (Dees et al., 1981) and whole-cell protein analysis (Holmes et al., 1990). Analysis of rRNA gene sequence similarities indicated that EF-4 strains were closely related to th ...
... In addition, both biotypes can be distinguished based on DNA G+C content (Bercovier et al., 1982), cellular fatty acid content (Dees et al., 1981) and whole-cell protein analysis (Holmes et al., 1990). Analysis of rRNA gene sequence similarities indicated that EF-4 strains were closely related to th ...
A Single Eubacterial Origin of Eukaryotic
... were used to amplify a fragment of 1,539 bp, which was cloned into pGEMT (Promega). Two clones were sequenced on both strands. To distinguish between this PCR product and the published G. lamblia PFO gene sequence, we refer to the published sequence as G. lamblia PFO1 and to the gene represented by ...
... were used to amplify a fragment of 1,539 bp, which was cloned into pGEMT (Promega). Two clones were sequenced on both strands. To distinguish between this PCR product and the published G. lamblia PFO gene sequence, we refer to the published sequence as G. lamblia PFO1 and to the gene represented by ...
background of the invention
... measurement of interactions between special pathogens such as viruses, bacteria and other biomolecules. In addition, the biosensor can be miniaturized into a chip without requiring fluorescence or enzyme calibration, so that such biosensor provides a convenient way for patients to carry the biosenso ...
... measurement of interactions between special pathogens such as viruses, bacteria and other biomolecules. In addition, the biosensor can be miniaturized into a chip without requiring fluorescence or enzyme calibration, so that such biosensor provides a convenient way for patients to carry the biosenso ...
Selection for TnlO Tet Repressor Binding to tet Operator
... is represented by a linear bar with both the N- and C-terminal ends indicated. The solid portion defines the potential a-helix-turn-ahelix motif, which is thought to be involved in DNA binding (amino acid residues 26 to 47; ISACKSON and BERTRAND1985). The region of the protein for which mutants have ...
... is represented by a linear bar with both the N- and C-terminal ends indicated. The solid portion defines the potential a-helix-turn-ahelix motif, which is thought to be involved in DNA binding (amino acid residues 26 to 47; ISACKSON and BERTRAND1985). The region of the protein for which mutants have ...
Couchioplanes caeruleus - International Journal of Systematic and
... microscope (model JSM-5400; JEOL Ltd.). Samples for scanning electron microscopy were prepared by cutting a block from the agar on which a culture grew, fixing the block in osmium tetroxide vapor at room temperature for 4 h, dehydrating the cells through a graded ethanol series and then in a Hitachi ...
... microscope (model JSM-5400; JEOL Ltd.). Samples for scanning electron microscopy were prepared by cutting a block from the agar on which a culture grew, fixing the block in osmium tetroxide vapor at room temperature for 4 h, dehydrating the cells through a graded ethanol series and then in a Hitachi ...
Control of DNA excision efficiency in Paramecium
... micronuclei undergo meiosis, whereas the macronuclei degenerate. The fusion of two gametic nuclei produces a zygotic nucleus. This nucleus divides twice and the daughter nuclei then differentiate into a micronucleus or a macronucleus. In the second case, the whole genome is processed through chromos ...
... micronuclei undergo meiosis, whereas the macronuclei degenerate. The fusion of two gametic nuclei produces a zygotic nucleus. This nucleus divides twice and the daughter nuclei then differentiate into a micronucleus or a macronucleus. In the second case, the whole genome is processed through chromos ...
Trichothiodystrophy: Update on the sulfur
... Trichothiodystrophy (TTD) refers to a heterogeneous group of autosomal recessive disorders that share the distinctive features of short, brittle hair and an abnormally low sulfur content. Within the spectrum of the TTD syndromes are numerous interrelated neuroectodermal disorders. The TTD syndromes ...
... Trichothiodystrophy (TTD) refers to a heterogeneous group of autosomal recessive disorders that share the distinctive features of short, brittle hair and an abnormally low sulfur content. Within the spectrum of the TTD syndromes are numerous interrelated neuroectodermal disorders. The TTD syndromes ...