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Stable Nuclear Transformation of the diatom Phaeodactylum
Stable Nuclear Transformation of the diatom Phaeodactylum

... diatom enables the manipulation of the gene structure and the regulation of the genome.  With the ability to perform a nuclear transformation, a new door is open that allows diatoms to be mass produced in a laboratory ...
mc2 Genome_Organization
mc2 Genome_Organization

... SINEs (short interspersed nuclear elements) are very small: 100-400 bp. They contain internal promoters for RNA polymerase 3. Several families, some originated as tRNA genes and others as 7SL RNA, the RNA involved in the signal recognition particle that guides secreted and membrane protein translati ...
Genome projects and model organisms
Genome projects and model organisms

... elegans and 7.5 times smaller than human genome Homologous genes in Fugu and mammals show conserved synteny: Same exon-intron organisation Introns much smaller Useful for identifying conserved essential elements in vertebrate genomes ...
Polymerase Chain Reaction
Polymerase Chain Reaction

... Separation: Double Stranded DNA is denatured by heat into single strands. Short Primers for DNA replication are added to the mixture. DNA polymerase catalyzes the production of complementary new strands. Copying The process is repeated for each new strand created All three steps are carried out in t ...
Sbjct = Alu sequence
Sbjct = Alu sequence

genetic concepts
genetic concepts

... fertilization > restoration duplication of genetic information  one gene of male and one of female origin give rise to a particular feature ...
Lab - Protein Synthesis
Lab - Protein Synthesis

... The coding sequence (5’  3’ “antisense”) of DNA below leads to the production of a specific protein. That makes it a gene. The gene was sequenced from samples taken from healthy human patients. As a genetic researcher you must first transcribe the sequence into an mRNA sequence. Then, using the gen ...
Chromosomes in prokaryotes
Chromosomes in prokaryotes

... structure called chromatin. Chromosomes may exist as either duplicated or unduplicated—unduplicated chromosome is linear DNA molecule, whereas duplicated chromosome contains two copies of DNA joined by a centromere. In prokaryotes DNA is usually arranged as a circle, which is tightly coiled in, some ...
Lecture 20  DNA Repair and Genetic Recombination
Lecture 20 DNA Repair and Genetic Recombination

... loss or insertion of DNA bases. Three types of recombination: – Homologous recombination – also known as generalized; occurs at meiosis (as we have just discussed) or not…. – Site specific (specialized) recombination – typically in bacteria and viruses; enzymes involved act only on a particular pair ...
Replication, Transcription, and Translation
Replication, Transcription, and Translation

The timing of gene expression
The timing of gene expression

... for the formation of body structures (e.g. begin the gene cascade for limb formation) Hox 9-13 control limb development in birds and mammals ...
Junk DNA indicted - Creation Ministries International
Junk DNA indicted - Creation Ministries International

... have regulatory functions, and a large fraction of them are expression is within introns and antisense introns’ embedded in intron sequences: (emphasis added)?11 ‘Although many introns degrade, some con There have always been those who have wondered tain active elements, such as microRNAs that can ...
Document
Document

... including methylaton ...
DOC
DOC

... that is used to decode its genetic code and use the information to make proteins. Genes are made of DNA. The expression of DNA is protein. The term given for making a protein is called “protein synthesis.” This requires DNA to provide the coded genetic information, the three types of RNA, and the am ...
8.6 Gene Expression and Regulation
8.6 Gene Expression and Regulation

... These proteins interact with operator sections of genes Function to control gene expression Different than in bacteria because structural proteins are not linked together in operons- they may be far apart or on different chromosomes •Introns Introns- intervening sequences of bases within genes t ...
Document
Document

... First evidence by Lederberg and Tatum 1946 - mixed two auxotrophic strains for several hours in rich medium than plated on minimal medium. Only recombinants which are now Prototrophic will be able to grow Second evidence- U tube experiment proved direct cell contact was required During this process ...
GENETICS SOL REVIEW – 2015 PART II  Name  ____________________________
GENETICS SOL REVIEW – 2015 PART II Name ____________________________

... Coiled DNA found in nucleus; structure containing DNA and proteins Process of cells reduction division of chromosomes; produces gametes Reproductive cell; eggs and sperm Specific characteristics Traits determined by genes located on the X chromosome In the first meiotic division chromosomes exchange ...
BSC 219
BSC 219

... initiation complex that recruits RNA Polymerase to the promoter region. The DNA sequences and some proteins in the complex are variable between promoters. Prokaryotic initiation relies only upon the relative strength of the promoter sequences at recruiting Sigma factor and RNA Polymerase to the prom ...
Full Text
Full Text

... Venter’s DNA sequence—as a diploid genome sequence—was released in September 2007 (9). The distinction merits clarification, because the Human Genome Sequencing Consortium assembly was a composite sequence of haploids derived from several individuals, and the Celera Genome was a consensus sequence d ...
Biotechnology toolkit part 1 File
Biotechnology toolkit part 1 File

... This is done by mechanically breaking the cells open, then using detergents and enzymes to break down the cell walls and membranes. The detergents also break down the nuclear membrane releasing the DNA. Step 2: remove the unwanted cell debris This is done by filtering the extract or by centrifuging ...
Christ The King School Exampro A-level Biology (7401/7402) DNA
Christ The King School Exampro A-level Biology (7401/7402) DNA

Presentation
Presentation

... sequence and arrangement of amino acids. 3. Amino acids are attached to one another by peptide bonds to form polypeptide chains. 4. The 3D form of the protein determines ...
What is PKU? - cloudfront.net
What is PKU? - cloudfront.net

... The human genome contains about 3 billion base pairs, of which only about 3% constitute expressed genes. The non coding regions of the genome vary tremendously and may differ between individuals by changes in single base pairs of different numbers of repeats of a particular base pair sequence. DNA ...
Genes and How they work!
Genes and How they work!

... about 250 A’s to 3’ end  long A tail. Needed to prevent degradation. ...
Gene Mutations
Gene Mutations

... Bell Ringer 3.24 1. Where does translation occur in the cell? 2. Why do we need RNA to make proteins? 3. How can different codons code for the same amino acid? ...
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Non-coding DNA

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