... generated from the polyk mRNA of this tumor (Fig. 3E). These observations indicated that the two classes of clones corresponded to the transcripts coding for the most basic and the most acidic 29-kDa proteins. For the first group of hepatocellular carcinoma cDNA clones, sequence information was obta ...
Why Mitochondrial Genes are Most Often Found in Nuclei
... of these have originated from the proteobacterial ancestor, but a significant fraction have arisen within the eukaryotic genome (Andersson and Kurland 1999; unpublished data). In the yeast Saccharomyces cerevisiae, in which the nucleus encodes more than 400 mitochondrial proteins, at most half of th ...
... of these have originated from the proteobacterial ancestor, but a significant fraction have arisen within the eukaryotic genome (Andersson and Kurland 1999; unpublished data). In the yeast Saccharomyces cerevisiae, in which the nucleus encodes more than 400 mitochondrial proteins, at most half of th ...
Sequence Heterogeneities Among 16s
... different but closely related genera (enterobacteria) and for different species or strains within a single genus (Escherichia or Salmonella), and finally how similar are the ribosomal operons within a single organism (Escherichia coli). These sequences have been analyzed by neighbor-joining, maximum ...
... different but closely related genera (enterobacteria) and for different species or strains within a single genus (Escherichia or Salmonella), and finally how similar are the ribosomal operons within a single organism (Escherichia coli). These sequences have been analyzed by neighbor-joining, maximum ...
The energetic basis of the DNA double helix: a
... rather controversial in a number of aspects. The first DSC studies of natural DNA suggested that their unfolding/dissociation proceeds without noticeable heat capacity increment, Cp (9). Since according to Kirchhoff’s relation, Cp = ∂(H)/∂T, it was assumed, and widely accepted, that the enthalpy ...
... rather controversial in a number of aspects. The first DSC studies of natural DNA suggested that their unfolding/dissociation proceeds without noticeable heat capacity increment, Cp (9). Since according to Kirchhoff’s relation, Cp = ∂(H)/∂T, it was assumed, and widely accepted, that the enthalpy ...
DNA Polyacrylamide Gel Electrophoresis
... 7. After polymerization is complete, surround the comb and the top of the gel with paper towels that have been soaked in 1x TBE. Then seal the entire gel in Saran Wrap or plastic bag and store it at 4°C until needed. 8. When ready to proceed with electrophoresis, remove gels from gel caster, careful ...
... 7. After polymerization is complete, surround the comb and the top of the gel with paper towels that have been soaked in 1x TBE. Then seal the entire gel in Saran Wrap or plastic bag and store it at 4°C until needed. 8. When ready to proceed with electrophoresis, remove gels from gel caster, careful ...
Lampetra fluviatilis Neurotrophin Homolog, Descendant of a
... each site as an independent character. Sequences were imported to PAUP v. 3.1.1 (Swofford, 1993) for an unbiased parsimonious analysis. The neurotrophin and Trk matrices were analyzed using unweighted characters and with the exclusion of uninformative characters. The phylogenetic trees were generate ...
... each site as an independent character. Sequences were imported to PAUP v. 3.1.1 (Swofford, 1993) for an unbiased parsimonious analysis. The neurotrophin and Trk matrices were analyzed using unweighted characters and with the exclusion of uninformative characters. The phylogenetic trees were generate ...
ANALYSIS OF MULTIPLE RESTRICTION FRAGMENT LENGTH
... fragments in the FF individuals; therefore, an allelic Sac I fragment could not be identified (Fig . 4) . Sac I also generated a CRI-1 +, CRI-4'}, 7 .9-kb restriction fragment that was present only in the DNA of the four FF donors and two FS donors who exhibited the CRI-1+, GBIR1+, 14 .5-kb Bam HI f ...
... fragments in the FF individuals; therefore, an allelic Sac I fragment could not be identified (Fig . 4) . Sac I also generated a CRI-1 +, CRI-4'}, 7 .9-kb restriction fragment that was present only in the DNA of the four FF donors and two FS donors who exhibited the CRI-1+, GBIR1+, 14 .5-kb Bam HI f ...
DNA-Based Methods for the Identification of Commercial Fish and
... with highly processed samples (Lenstra 2003). Although DNA molecules can degrade during processing, they are more thermostable than proteins: DNA fragments as long as 300 bp can still be recovered following sterilization (Chapela and others 2007). Also, DNA has the potential to provide a greater amo ...
... with highly processed samples (Lenstra 2003). Although DNA molecules can degrade during processing, they are more thermostable than proteins: DNA fragments as long as 300 bp can still be recovered following sterilization (Chapela and others 2007). Also, DNA has the potential to provide a greater amo ...
sacB - GENI
... We have designed the core of a curriculum, based on genetic complementation of defined E. coli mutants, that enables true experimentation in the classroom The modular format allows testing completely different genes every semester but does so with repetitive sets of protocols and materials Consisten ...
... We have designed the core of a curriculum, based on genetic complementation of defined E. coli mutants, that enables true experimentation in the classroom The modular format allows testing completely different genes every semester but does so with repetitive sets of protocols and materials Consisten ...
Product Information FluoroVueTM Nucleic Acid Gel Stain (10,000X
... Fig. 2. The FluoroVueTM Nucleic Acid Gel Stain shows a green-yellow fluorescence under blue light excitation. The sensitivity of NS1000 is about 0.14 ng (arrow) for a 4 kb fragment. Caution Dispose of the stain in accordance to local rules and regulations. The fluorescent staining dye stock solutio ...
... Fig. 2. The FluoroVueTM Nucleic Acid Gel Stain shows a green-yellow fluorescence under blue light excitation. The sensitivity of NS1000 is about 0.14 ng (arrow) for a 4 kb fragment. Caution Dispose of the stain in accordance to local rules and regulations. The fluorescent staining dye stock solutio ...
DNA breathing dynamics distinguish binding from nonbinding
... identifies two probable YY1 consensus binding sites that are centered at positions 243 and 18 upstream of the transcriptional start site (TSS). We assembled gel shift reactions with recombinant YY1 protein and the 33P-labeled 18 bp long 243YY1 and 18YY1 PLG oligonucleotides comprising the YY1 consens ...
... identifies two probable YY1 consensus binding sites that are centered at positions 243 and 18 upstream of the transcriptional start site (TSS). We assembled gel shift reactions with recombinant YY1 protein and the 33P-labeled 18 bp long 243YY1 and 18YY1 PLG oligonucleotides comprising the YY1 consens ...
Review Molecular Biology in Arteriosclerosis Research
... strategies used to identify the particular clones. The avian very low density lipoprotein (VLDL) apoprotein (apo) II cDNA clone was the first apolipoprotein clone isolated.45 In this case, the cDNA was prepared from highly purified apo II mRNA, thereby simplifying the identification of the cDNA clon ...
... strategies used to identify the particular clones. The avian very low density lipoprotein (VLDL) apoprotein (apo) II cDNA clone was the first apolipoprotein clone isolated.45 In this case, the cDNA was prepared from highly purified apo II mRNA, thereby simplifying the identification of the cDNA clon ...
Parallel Genetic and Phenotypic Evolution of DNA Superhelicity in
... although the populations had different alleles at each locus. Statistical analyses of these polymorphisms implied the action of positive selection and, moreover, suggested that fis and dusB, which belong to the same operon, have related functions. Indeed, we demonstrated that DusB regulates the expr ...
... although the populations had different alleles at each locus. Statistical analyses of these polymorphisms implied the action of positive selection and, moreover, suggested that fis and dusB, which belong to the same operon, have related functions. Indeed, we demonstrated that DusB regulates the expr ...
生物信息学主要英文术语及释义
... state of a hid-den Markov model (representing one column of a multiple sequence alignment of proteins), based on prior distributions found in conserved protein domains (blocks). Distance in sequence analysis（序列距离） The number of observed changes in an optimal alignment of two sequences, usually not c ...
... state of a hid-den Markov model (representing one column of a multiple sequence alignment of proteins), based on prior distributions found in conserved protein domains (blocks). Distance in sequence analysis（序列距离） The number of observed changes in an optimal alignment of two sequences, usually not c ...
final1-eu-marie-curie-final-report
... A sustainable supply of marine natural products for potential therapeutics is one of the greatest challenges facing drug discovery efforts today, especially during clinical trials. Nearly 300 compounds with therapeutic potential have been isolated from the tropical marine cyanobacterium Lyngbya maju ...
... A sustainable supply of marine natural products for potential therapeutics is one of the greatest challenges facing drug discovery efforts today, especially during clinical trials. Nearly 300 compounds with therapeutic potential have been isolated from the tropical marine cyanobacterium Lyngbya maju ...
Candidate gene analysis of thyroid hormone receptors
... of six), the two unique TRb alleles were associated with metamorphic populations. Overall, these data indicate no general relationship between life cycle mode and TR allelic variation among populations or species. High sequence similarity was found among the alleles of each TR gene; pairwise dieren ...
... of six), the two unique TRb alleles were associated with metamorphic populations. Overall, these data indicate no general relationship between life cycle mode and TR allelic variation among populations or species. High sequence similarity was found among the alleles of each TR gene; pairwise dieren ...
induction of instability at selected loci in maize
... 1:3 ratio will occur if the plants are not homozygous for the mutation (i.e. there is only one a1m‐4). 1/2 of gametes wil posses Ac, while only 1/4 of the gametes will possess the mutation. Therefore, less kernels will the mutation in which Ds can "jump out" of, producing less variegated kernels. 2 ...
... 1:3 ratio will occur if the plants are not homozygous for the mutation (i.e. there is only one a1m‐4). 1/2 of gametes wil posses Ac, while only 1/4 of the gametes will possess the mutation. Therefore, less kernels will the mutation in which Ds can "jump out" of, producing less variegated kernels. 2 ...
Messenger RNA
... Unstable, has a half-life of only a few minutes. Transcription and translation similar rate ...
... Unstable, has a half-life of only a few minutes. Transcription and translation similar rate ...
