Designing synthetic MLPA probes - MRC
... Designing synthetic MLPA probes 1. Introduction Designing synthetic probes is a common practice performed by many of our customers who want to have an MLPA probemix targeted to genes not covered by any probemix available from MRC-Holland. To facilitate this, we have a protocol available that describ ...
... Designing synthetic MLPA probes 1. Introduction Designing synthetic probes is a common practice performed by many of our customers who want to have an MLPA probemix targeted to genes not covered by any probemix available from MRC-Holland. To facilitate this, we have a protocol available that describ ...
1 FRQ-interacting RNA Helicase (FRH) Mediates Negative and
... respectively (DUNLAP 2008; YOUNG and KAY 2001). Since then, research on many model organisms has revealed a conserved aspect of regulation in which negative feedback plays an essential role in maintaining a self-sustained circadian clock (Dunlap 1999; Heintzen and Liu 2007; Mackey 2007). The circadi ...
... respectively (DUNLAP 2008; YOUNG and KAY 2001). Since then, research on many model organisms has revealed a conserved aspect of regulation in which negative feedback plays an essential role in maintaining a self-sustained circadian clock (Dunlap 1999; Heintzen and Liu 2007; Mackey 2007). The circadi ...
OLSON LAB PROTOCOL: Agarose Gel Electrophoresis using GelRed
... • Larger fragments move more slowly through the agarose gel matrix than small fragments and hence the fragments are size fractionated • The resolution of separation depends on the type and concentration of gel and the length of the run: ‚mini‘ gels are fairly low resolution and typically used for se ...
... • Larger fragments move more slowly through the agarose gel matrix than small fragments and hence the fragments are size fractionated • The resolution of separation depends on the type and concentration of gel and the length of the run: ‚mini‘ gels are fairly low resolution and typically used for se ...
18. The Reference Sequence (RefSeq) Project
... The goal of the NCBI RefSeq [http://www.ncbi.nih.gov/RefSeq/] project is to provide the best nonredundant and comprehensive collection of naturally occurring DNA, RNA, and protein molecules for major organisms. The collection explicitly links the nucleotide and protein sequences. Ideally, all molecu ...
... The goal of the NCBI RefSeq [http://www.ncbi.nih.gov/RefSeq/] project is to provide the best nonredundant and comprehensive collection of naturally occurring DNA, RNA, and protein molecules for major organisms. The collection explicitly links the nucleotide and protein sequences. Ideally, all molecu ...
Causes that Make a Difference - Philsci
... actually made the difference, such as Mary’s striking the match. This distinction is elusive, and it is tempting to dismiss it as illusory. On this view, there is an ontological difference between causes and non-causes, but there is no ontological difference among causes. This was the position taken ...
... actually made the difference, such as Mary’s striking the match. This distinction is elusive, and it is tempting to dismiss it as illusory. On this view, there is an ontological difference between causes and non-causes, but there is no ontological difference among causes. This was the position taken ...
Puffs and PCR: the in vivo dynamics of early gene
... in Wang et al., (1989), Chelly et al., (1990), Makino et al., (1990) and Innis et al., (1990). (a) Design of primers. In order to standardise reaction conditions, we used 20-mer oligonucleotide primers (with a few exceptions, see Table 1), having a GC content as close to 50% as possible. In each cas ...
... in Wang et al., (1989), Chelly et al., (1990), Makino et al., (1990) and Innis et al., (1990). (a) Design of primers. In order to standardise reaction conditions, we used 20-mer oligonucleotide primers (with a few exceptions, see Table 1), having a GC content as close to 50% as possible. In each cas ...
Investigating the link between tRNA and mRNA - EMBL-EBI
... The genetic code describes how a sequence of codons on an mrna is translated into a sequence of amino acids, forming a protein. The genetic code manifests itself in the cell as trna molecules, which fall into several classes of anticodon isoacceptors, each decoding a single codon into its correspond ...
... The genetic code describes how a sequence of codons on an mrna is translated into a sequence of amino acids, forming a protein. The genetic code manifests itself in the cell as trna molecules, which fall into several classes of anticodon isoacceptors, each decoding a single codon into its correspond ...
Analysis of the genetic potential of the spongederived fungus
... lab and for introducing me into the puzzling world of polyketides. I am grateful for all suggestions that I got from him during the practical work in the lab, as well as for the writing of my thesis. Moreover, I want to thank him for his trust and unique experience in building up a laboratory togeth ...
... lab and for introducing me into the puzzling world of polyketides. I am grateful for all suggestions that I got from him during the practical work in the lab, as well as for the writing of my thesis. Moreover, I want to thank him for his trust and unique experience in building up a laboratory togeth ...
International Agency for Research on Cancer (IARC)
... Mixtures of aflatoxins and aflatoxin B1 have been tested extensively for carcinogenicity by various routes of administration in several strains of mice and rats, in hamsters, several strains of fish, ducks, tree shrews and monkeys. Following their oral administration, mixtures of aflatoxins and afla ...
... Mixtures of aflatoxins and aflatoxin B1 have been tested extensively for carcinogenicity by various routes of administration in several strains of mice and rats, in hamsters, several strains of fish, ducks, tree shrews and monkeys. Following their oral administration, mixtures of aflatoxins and afla ...
View PDF - OSU Biochemistry and Molecular Biology
... Hox function, but also to elucidate the mechanisms of Hox target gene selection and regulation. In this context, chromatin immunoprecipitation (ChIP) was developed, which has become one of the most powerful tools for the study of protein–DNA interactions in vivo. Before genomic arrays or massively p ...
... Hox function, but also to elucidate the mechanisms of Hox target gene selection and regulation. In this context, chromatin immunoprecipitation (ChIP) was developed, which has become one of the most powerful tools for the study of protein–DNA interactions in vivo. Before genomic arrays or massively p ...
Novel pathogen-specific primers for the detection of Agrobacterium
... (BURR et al. 1998, BURR and OTTEN 1999). The latter ones have a unique structure and T-DNA organization only distantly related to the most common octopine and nopaline type plasmids (CANADAY et al. 1992, GÉRARD et al. 1992). Since the pathogen systemically infects its host, propagating materials do ...
... (BURR et al. 1998, BURR and OTTEN 1999). The latter ones have a unique structure and T-DNA organization only distantly related to the most common octopine and nopaline type plasmids (CANADAY et al. 1992, GÉRARD et al. 1992). Since the pathogen systemically infects its host, propagating materials do ...
Defining the Schistosoma haematobium kinome enables the
... and can, together with other factors, induce malignant bladder cancer9. As there is no effective vaccine against schistosomiasis, current treatment relies on a single drug, praziquantel10. With increased efforts to control this disease by mass treatment, the possibility of praziquantel resistance de ...
... and can, together with other factors, induce malignant bladder cancer9. As there is no effective vaccine against schistosomiasis, current treatment relies on a single drug, praziquantel10. With increased efforts to control this disease by mass treatment, the possibility of praziquantel resistance de ...
GC-content of synonymous codons profoundly influences amino
... could be related to the different amino acid usage patterns in organisms. In order to address this question, we focused on the relationship between GCsyn, regional GC-content, and amino acid usage in different genomes, hypothesizing that the amino acids with high GCsyn could be used more frequently ...
... could be related to the different amino acid usage patterns in organisms. In order to address this question, we focused on the relationship between GCsyn, regional GC-content, and amino acid usage in different genomes, hypothesizing that the amino acids with high GCsyn could be used more frequently ...
The Myriad case (Association for Molecular Pathology v
... • Native BRCA1/2 resides on chromosomes 17/13 part of millions of nucleotides • Isolated BRCA1/2 – exons only covering as few as 15 to about 10000 nucleotides not found in nature © 2011 Connolly Bove Lodge & Hutz LLP • Focus is on structure Myriad Slip Op. at 42 © 2011 Connolly Bove Lodge & Hutz ...
... • Native BRCA1/2 resides on chromosomes 17/13 part of millions of nucleotides • Isolated BRCA1/2 – exons only covering as few as 15 to about 10000 nucleotides not found in nature © 2011 Connolly Bove Lodge & Hutz LLP • Focus is on structure Myriad Slip Op. at 42 © 2011 Connolly Bove Lodge & Hutz ...
A MIAME-compliant Microarray Database
... • In the first experiment, they found some genes regulated by addition of GA-4 • They made GFP fusions to three of these genes ...
... • In the first experiment, they found some genes regulated by addition of GA-4 • They made GFP fusions to three of these genes ...
Genetic Codes with No Dedicated Stop Codon: Context
... here. Surprisingly, in two of these species, we find efficient translation of all 64 codons as standard amino acids and recognition of either one or all three stop codons. How, therefore, does the translation machinery interpret a ‘‘stop’’ codon? We provide evidence, based on ribosomal profiling and ...
... here. Surprisingly, in two of these species, we find efficient translation of all 64 codons as standard amino acids and recognition of either one or all three stop codons. How, therefore, does the translation machinery interpret a ‘‘stop’’ codon? We provide evidence, based on ribosomal profiling and ...
New variants of the tet(M) gene in Clostridium
... tet(M)1, 2, 3 and 4 variants were found in C. difficile isolates resistant or inducibly resistant to tetracycline, and tet(M)5 and 6 were found in susceptible isolates. The organization of the element carrying tet(M)3, tet(M)6 and tet(M)1 of cd12 was similar to that of E. faecalis DS16. This analysi ...
... tet(M)1, 2, 3 and 4 variants were found in C. difficile isolates resistant or inducibly resistant to tetracycline, and tet(M)5 and 6 were found in susceptible isolates. The organization of the element carrying tet(M)3, tet(M)6 and tet(M)1 of cd12 was similar to that of E. faecalis DS16. This analysi ...
Microbial Genetics, Mutation and Repair Answer the following
... 7. Genetic information transferred from one bacteria to another using a pilus is known as _____________. 8. F+ cells contain a fertility _____________. 9. An ____________ plasmid contains both the genetic information needed for a sex pilus as well as parts of the bacterial chromosome. 10. Plasmids t ...
... 7. Genetic information transferred from one bacteria to another using a pilus is known as _____________. 8. F+ cells contain a fertility _____________. 9. An ____________ plasmid contains both the genetic information needed for a sex pilus as well as parts of the bacterial chromosome. 10. Plasmids t ...
Specific and ubiquitous expression of different Zn finger protein
... specific DNA binding (31). Furthermore,they lack the conserved Phe and Leu residues in the finger loop and the H-C link peptide TGEKP. Interestingly, when Kruppel(Kr), the Drosophila segmentation gene of the gap class(32) was isolated and sequenced.it was found to contain 5 Zn fingers(5),thereby imp ...
... specific DNA binding (31). Furthermore,they lack the conserved Phe and Leu residues in the finger loop and the H-C link peptide TGEKP. Interestingly, when Kruppel(Kr), the Drosophila segmentation gene of the gap class(32) was isolated and sequenced.it was found to contain 5 Zn fingers(5),thereby imp ...
Chromosomes in Saccharomyces cerevisiae
... FIG. 1. Circularly permuted version of chromosome III. (a) Construction of the linear chromosome III-Tr. From top to bottom the figure shows: the normal version of chromosome III; the circular derivative of chromosome III (4); chromosome III-Tr which results from the resolution of atn inverted repea ...
... FIG. 1. Circularly permuted version of chromosome III. (a) Construction of the linear chromosome III-Tr. From top to bottom the figure shows: the normal version of chromosome III; the circular derivative of chromosome III (4); chromosome III-Tr which results from the resolution of atn inverted repea ...