Editing Multiple Alignments
... Secondary structure elements Hydrophobic regions, polar residues ...
... Secondary structure elements Hydrophobic regions, polar residues ...
Genetic Polymorphism of Human CYP2E1
... To evaluate the functional importance of the mutations in the CYP2E1*2 and CYP2E1*3 alleles, site-directed mutagenesis was used to introduce these mutations into the wild-type CYP2E1 cDNA. The cDNAs were inserted into the pCMV4 expression vector and subsequently expressed in COS-1 cells. For compari ...
... To evaluate the functional importance of the mutations in the CYP2E1*2 and CYP2E1*3 alleles, site-directed mutagenesis was used to introduce these mutations into the wild-type CYP2E1 cDNA. The cDNAs were inserted into the pCMV4 expression vector and subsequently expressed in COS-1 cells. For compari ...
Gene Section ID4 (inhibitor of DNA binding 4, dominant negative helix-loop-helix protein)
... of the cell cycle machinery. Other ID genes are not redondant with ID4 during telencephalic develop-ment, supporting the idea that ID4 function is unique in this context (Yun et al., 2004). In immature neurons with high expression of ID proteins, heterodimers of bHLHID prevent DNA binding and expres ...
... of the cell cycle machinery. Other ID genes are not redondant with ID4 during telencephalic develop-ment, supporting the idea that ID4 function is unique in this context (Yun et al., 2004). In immature neurons with high expression of ID proteins, heterodimers of bHLHID prevent DNA binding and expres ...
PDF
... Polycomb Group (PcG) proteins mediate chromatin repression in plants and animals by catalyzing H3K27 methylation and H2AK118/119 mono-ubiquitination through the activity of the Polycomb repressive complex 2 (PRC2) and PRC1, respectively. PcG proteins were extensively studied in higher plants, but th ...
... Polycomb Group (PcG) proteins mediate chromatin repression in plants and animals by catalyzing H3K27 methylation and H2AK118/119 mono-ubiquitination through the activity of the Polycomb repressive complex 2 (PRC2) and PRC1, respectively. PcG proteins were extensively studied in higher plants, but th ...
SPT3 interacts with TFIID to allow normal transcription in
... spt3 sup alleles were used: one strong allele, spt3-401, and three weak alleles, spt3-417, spt3-426, and spt3-445. For each case examined, the spt3 ~up m u t a t i o n s suppressed the transcriptional defects caused by sptl5-21 (Fig. 2). For his4-9176, previous work demonstrated that sptl 521 suppre ...
... spt3 sup alleles were used: one strong allele, spt3-401, and three weak alleles, spt3-417, spt3-426, and spt3-445. For each case examined, the spt3 ~up m u t a t i o n s suppressed the transcriptional defects caused by sptl5-21 (Fig. 2). For his4-9176, previous work demonstrated that sptl 521 suppre ...
WS Procs 975 x 65
... and a more realistic motif model should be able to describe nucleotide interdependence. Man and Stormo [18] have arrived at a similar conclusion in their analysis of Salmonella bacteriophage repressor Mnt: they found that interactions of Mnt with nucleotides at positions 16 and 17 of the 21 bp bindi ...
... and a more realistic motif model should be able to describe nucleotide interdependence. Man and Stormo [18] have arrived at a similar conclusion in their analysis of Salmonella bacteriophage repressor Mnt: they found that interactions of Mnt with nucleotides at positions 16 and 17 of the 21 bp bindi ...
Genetic mapping of mutations using phenotypic pools and
... require gene expression. We have demonstrated a molecular approach to the mapping of mutant genes using RAPD markers and pooling of Individuals based on phenotype. To map genes by phenotypic pooling a strain carrying a mutation is crossed to a strain that is homozygous for the wild-type allele of th ...
... require gene expression. We have demonstrated a molecular approach to the mapping of mutant genes using RAPD markers and pooling of Individuals based on phenotype. To map genes by phenotypic pooling a strain carrying a mutation is crossed to a strain that is homozygous for the wild-type allele of th ...
lecture - Berkeley MCB
... regulation, is really like • Trans-acting factors do not distribute in the nucleus based on the primary sequence of the genome: some factors fail to bind most genes that have sequences waiting for them, and other factors bind a large number of genes that do NOT have sequences for them • Even when a ...
... regulation, is really like • Trans-acting factors do not distribute in the nucleus based on the primary sequence of the genome: some factors fail to bind most genes that have sequences waiting for them, and other factors bind a large number of genes that do NOT have sequences for them • Even when a ...
Picoplankton Bloom in Global South? A High Fraction of Aerobic
... Lami et al., 2007; Schwalbach and Fuhrman, 2005) and despite initial reports, they support the hypothesis proposed by Kolber (2001) that these organisms would have an advantage in oligotrophic conditions. Recent studies, however, suggest that AAPs thrive better in more eutrophic environments (Cottre ...
... Lami et al., 2007; Schwalbach and Fuhrman, 2005) and despite initial reports, they support the hypothesis proposed by Kolber (2001) that these organisms would have an advantage in oligotrophic conditions. Recent studies, however, suggest that AAPs thrive better in more eutrophic environments (Cottre ...
NUCLEIC ACID ECONOMY IN BACTERIA INFECTED WITH
... survival of 10-~) in chilled centrifuge tubes. After about 30 minutes in an ice bath, the tubes were warmed 30 minutes at 37°C. and refrigerated overnight. The next morning I0 #g. desoxyribonuclease was added to each tube and the contents were diluted to 10 ml. with 0.1 per cent peptone water. After ...
... survival of 10-~) in chilled centrifuge tubes. After about 30 minutes in an ice bath, the tubes were warmed 30 minutes at 37°C. and refrigerated overnight. The next morning I0 #g. desoxyribonuclease was added to each tube and the contents were diluted to 10 ml. with 0.1 per cent peptone water. After ...
Chapter 29 Slides
... Genetic Information Can Be Transferred Between Bacteria • In 1946, Lederberg and Tatum showed that two different strains of bacteria with different growth requirements could exchange genes • Lederberg and Tatum surmised that the bacterial cells must interact with each other - the process is now know ...
... Genetic Information Can Be Transferred Between Bacteria • In 1946, Lederberg and Tatum showed that two different strains of bacteria with different growth requirements could exchange genes • Lederberg and Tatum surmised that the bacterial cells must interact with each other - the process is now know ...
Centromere Locations and Associated Chromosome
... the centromere (CEN) regions of A. thaliana, using at least two genes from the short and long arms of each chromosome. In our map, genes from all 10 A. thaliana chromosome arms are also tightly linked in A. lyrata. Genes from the regions on the two sides of CEN5 have distant map localizations in A. ...
... the centromere (CEN) regions of A. thaliana, using at least two genes from the short and long arms of each chromosome. In our map, genes from all 10 A. thaliana chromosome arms are also tightly linked in A. lyrata. Genes from the regions on the two sides of CEN5 have distant map localizations in A. ...
5 Genetic Analysis of Kidney Disease in Mice
... The process of identifying the genes underlying QTLs can be accelerated by using a variety of statistical and bioinformatics techniques, which we will illustrate using examples from several phenotypes. We begin by selecting QTL found in three or more crosses that also had a concordant human QTL. We ...
... The process of identifying the genes underlying QTLs can be accelerated by using a variety of statistical and bioinformatics techniques, which we will illustrate using examples from several phenotypes. We begin by selecting QTL found in three or more crosses that also had a concordant human QTL. We ...
View Tutorial
... and accumulate over evolutionary time. As a result, the same genes compared across multiple species will not be identical, but will display insertions, deletions, or substitutions of nucleotides. Since DNA codes for protein, similar changes occur in protein sequences. Over long periods of time, subs ...
... and accumulate over evolutionary time. As a result, the same genes compared across multiple species will not be identical, but will display insertions, deletions, or substitutions of nucleotides. Since DNA codes for protein, similar changes occur in protein sequences. Over long periods of time, subs ...
The Effects of Deleterious Mutations on Evolution at
... descendants of all other classes are ultimately destined for elimination. This is equivalent to saying that the effective population size Ne (Wright 1931) is equal to the number of breeding individuals in this “least-loaded” class and is necessarily much smaller than the number of breeding individua ...
... descendants of all other classes are ultimately destined for elimination. This is equivalent to saying that the effective population size Ne (Wright 1931) is equal to the number of breeding individuals in this “least-loaded” class and is necessarily much smaller than the number of breeding individua ...
Genome-Wide Analysis of In Vivo Binding of the Master Regulator
... function) sigma factors genes and some 70 two-component regulatory systems [4]. As producers of over half of known antibiotics, streptomycetes are a paradigm of secondary metaboliteproducing microorganisms [5,6]. Typically, antibiotic biosynthetic gene clusters are, at the very least, activated by a ...
... function) sigma factors genes and some 70 two-component regulatory systems [4]. As producers of over half of known antibiotics, streptomycetes are a paradigm of secondary metaboliteproducing microorganisms [5,6]. Typically, antibiotic biosynthetic gene clusters are, at the very least, activated by a ...
Journal of Bacteriology
... 248 that hybridizes with ropA revealed one large open reading frame of 1,074 bp encoding a mature protein of 38.096 kDa. Homology between this gene and ropA is 91.8% on the DNA level. Homology on the amino acid level is only 69.9% as a result of a frameshift. On the basis of homology and immunochemi ...
... 248 that hybridizes with ropA revealed one large open reading frame of 1,074 bp encoding a mature protein of 38.096 kDa. Homology between this gene and ropA is 91.8% on the DNA level. Homology on the amino acid level is only 69.9% as a result of a frameshift. On the basis of homology and immunochemi ...
Selection at the Wobble Position of Codons Read by the Same tRNA
... (Velculescu et al. 1997). These data, which were obtained under three different growth conditions (logarithmic, G2/M transition, and stationary), refer to a total of 60,633 yeast mRNAs, each represented by a sequence tag of 10 nt preceded by a four-base N1aIII restriction site. Tags associated with ...
... (Velculescu et al. 1997). These data, which were obtained under three different growth conditions (logarithmic, G2/M transition, and stationary), refer to a total of 60,633 yeast mRNAs, each represented by a sequence tag of 10 nt preceded by a four-base N1aIII restriction site. Tags associated with ...
Conjugative plasmids: vessels of the communal gene pool
... individual genomes by lipid membranes. A highly contributing factor to the extent of HGT is also the strikingly economical manner in which prokaryotic genomes are organized. There is usually very little genomic redundancy in prokaryotes, since a strong ‘deletion bias’ towards small genome sizes exis ...
... individual genomes by lipid membranes. A highly contributing factor to the extent of HGT is also the strikingly economical manner in which prokaryotic genomes are organized. There is usually very little genomic redundancy in prokaryotes, since a strong ‘deletion bias’ towards small genome sizes exis ...
Conjugative plasmids: vessels of the communal gene pool
... individual genomes by lipid membranes. A highly contributing factor to the extent of HGT is also the strikingly economical manner in which prokaryotic genomes are organized. There is usually very little genomic redundancy in prokaryotes, since a strong ‘deletion bias’ towards small genome sizes exis ...
... individual genomes by lipid membranes. A highly contributing factor to the extent of HGT is also the strikingly economical manner in which prokaryotic genomes are organized. There is usually very little genomic redundancy in prokaryotes, since a strong ‘deletion bias’ towards small genome sizes exis ...
A Complex Suite of Forces Drives Gene Traffic from Drosophila X
... genes from the X to the autosomes in both Drosophila melanogaster and humans (Betrán et al. 2002; Emerson et al. 2004; Potrzebowski et al. 2008). The autosomal copies of these paralogs tend to be testis expressed, suggesting that these new genes are preferentially retained because they allow for es ...
... genes from the X to the autosomes in both Drosophila melanogaster and humans (Betrán et al. 2002; Emerson et al. 2004; Potrzebowski et al. 2008). The autosomal copies of these paralogs tend to be testis expressed, suggesting that these new genes are preferentially retained because they allow for es ...