Altered adherence properties of a Streptococcus
... 1986), restriction sites (underlined in the primer sequences below) were incorporated into the primers designed to amplify the cshA promoter region. The primer pair comprised SMAPl (nucleotides 240-266, cshA locus), 5'-CTGCCCGGGATCGTGACTATCTATTTG-3', and SACP2 (complementary to nucleotides 619-645, ...
... 1986), restriction sites (underlined in the primer sequences below) were incorporated into the primers designed to amplify the cshA promoter region. The primer pair comprised SMAPl (nucleotides 240-266, cshA locus), 5'-CTGCCCGGGATCGTGACTATCTATTTG-3', and SACP2 (complementary to nucleotides 619-645, ...
Characterization of the neurohypophysial hormone gene loci in
... The presence of oxytocin in these distantly related vertebrates is the result of independent nucleotide substitutions in the two lineages. The neurohypophysial hormones are synthesized as part of a larger precursor molecule comprising a signal peptide, the nonapeptide hormone, and a neurophysin. The ...
... The presence of oxytocin in these distantly related vertebrates is the result of independent nucleotide substitutions in the two lineages. The neurohypophysial hormones are synthesized as part of a larger precursor molecule comprising a signal peptide, the nonapeptide hormone, and a neurophysin. The ...
Amplified fragment length polymorphism (AFLP)
... development. Genes that confer resistance to Hessian fly provide the most efficient and economical means of crop protection against this damaging insect. To date, 27 resistance genes (H1–H27) have been reported in wheat; among these, 11 are very effective in Morocco. In this study, we have utilized ...
... development. Genes that confer resistance to Hessian fly provide the most efficient and economical means of crop protection against this damaging insect. To date, 27 resistance genes (H1–H27) have been reported in wheat; among these, 11 are very effective in Morocco. In this study, we have utilized ...
The dystrophin / utrophin homologues in Drosophila and in sea urchin
... cDNA primer was from exon 4. The antisense PCR primer that consisted of exons 3±4 border sequences, and sense primers that were speci®c to each dmDLP isoform. The PCR reaction was stopped after 27 cycles. The products were size fractionated and transferred to nylon ®lters. After hybridization to int ...
... cDNA primer was from exon 4. The antisense PCR primer that consisted of exons 3±4 border sequences, and sense primers that were speci®c to each dmDLP isoform. The PCR reaction was stopped after 27 cycles. The products were size fractionated and transferred to nylon ®lters. After hybridization to int ...
Molecular Plant-Microbe Interacttions
... ments of interest were eluted, re-amplified by PCR with the same set of primers, and subsequently cloned into the pCR-2 vector plasmid. To minimize the number of false positives, a hybridization screen method was employed (Consalez et al. 1996). Six insert sequences scored positive. The sequences o ...
... ments of interest were eluted, re-amplified by PCR with the same set of primers, and subsequently cloned into the pCR-2 vector plasmid. To minimize the number of false positives, a hybridization screen method was employed (Consalez et al. 1996). Six insert sequences scored positive. The sequences o ...
Supplementary Methods Tables
... present a low risk of re-identification of individual participants. The open access data tier does not require user certification for data access. The controlled access tier contains data, including all raw sequence data, that is unique to the individual participant or for which there is a high risk ...
... present a low risk of re-identification of individual participants. The open access data tier does not require user certification for data access. The controlled access tier contains data, including all raw sequence data, that is unique to the individual participant or for which there is a high risk ...
little piggy
... “Yeah,” said John. Sue noticed he sounded a little tired. “There are a couple of research groups trying to figure out how to lower or eliminate the chemicals responsible for causing the odor. There are benefits in using intact males instead of barrows, namely lower feed costs and the meat is leaner. ...
... “Yeah,” said John. Sue noticed he sounded a little tired. “There are a couple of research groups trying to figure out how to lower or eliminate the chemicals responsible for causing the odor. There are benefits in using intact males instead of barrows, namely lower feed costs and the meat is leaner. ...
Kelly PD, Chu F, Woods IG, Ngo‑Hazelett P, Cardozo T, Huang H
... breeding protocol (Knapik et al. 1998; Shimoda et al. 1999). SSLPs segregate typically as codominant markers and they are more useful in diploid crosses than are dominant markers such as RAPDs. An advantage of the diploid protocol is that diploid zebrafish survive to adulthood, providing much more g ...
... breeding protocol (Knapik et al. 1998; Shimoda et al. 1999). SSLPs segregate typically as codominant markers and they are more useful in diploid crosses than are dominant markers such as RAPDs. An advantage of the diploid protocol is that diploid zebrafish survive to adulthood, providing much more g ...
Biological Chemistry
... Extended Response 12. The body has numerous energy reserves. What are they, and where are they found in the body? Which of these reserves provides the greatest source of quick energy? Answer: The body stores energy in the form of fat in adipose tissue, glycogen in the liver, and protein in muscle an ...
... Extended Response 12. The body has numerous energy reserves. What are they, and where are they found in the body? Which of these reserves provides the greatest source of quick energy? Answer: The body stores energy in the form of fat in adipose tissue, glycogen in the liver, and protein in muscle an ...
Genome Biology and
... Conserved core orthologues in vertebrates ¤ Core orthologues conserved in vertebrates have – Highly conserved protein sequences indicating that • They have been subject to purifying selection ...
... Conserved core orthologues in vertebrates ¤ Core orthologues conserved in vertebrates have – Highly conserved protein sequences indicating that • They have been subject to purifying selection ...
Strand A - hrsbstaff.ednet.ns.ca
... showed that they both had the sickle cell trait, they called Beth at the genetic counseling clinic at Children’s Hospital to talk about family planning. Beth told them that there is a very accurate prenatal test that can determine whether the developing fetus has sickle cell anemia, sickle trait, or ...
... showed that they both had the sickle cell trait, they called Beth at the genetic counseling clinic at Children’s Hospital to talk about family planning. Beth told them that there is a very accurate prenatal test that can determine whether the developing fetus has sickle cell anemia, sickle trait, or ...
Discussion paper - Office of the Gene Technology Regulator
... document? 3. Is there any scientific evidence that any of options 2-4 would result in a level of regulation not commensurate with risks posed by gene technology? 4. How might options 2-4 change the regulatory burden on you from the gene technology regulatory scheme? 5. How do you use item 1 of Sched ...
... document? 3. Is there any scientific evidence that any of options 2-4 would result in a level of regulation not commensurate with risks posed by gene technology? 4. How might options 2-4 change the regulatory burden on you from the gene technology regulatory scheme? 5. How do you use item 1 of Sched ...
The landscape of microbial phenotypic traits and associated genes
... not be represented in existing databases. To this end, we constructed a standard ‘bag-of-words’ representation: a matrix where rows correspond to words and columns to organisms for each of the five corpora separately (excluding the mixed collection). In order to enforce consistency between corpora, ...
... not be represented in existing databases. To this end, we constructed a standard ‘bag-of-words’ representation: a matrix where rows correspond to words and columns to organisms for each of the five corpora separately (excluding the mixed collection). In order to enforce consistency between corpora, ...
REVIEW ARTICLE Gene cassettes
... Hall, 1992b). Most 59-base elements have a central axis of symmetry as the consensus regions are imperfect inverted repeats. All 59-base elements also contain inverted repeat sequences in their central region, and it is this region that varies in length. Within the 59-base element family, a few grou ...
... Hall, 1992b). Most 59-base elements have a central axis of symmetry as the consensus regions are imperfect inverted repeats. All 59-base elements also contain inverted repeat sequences in their central region, and it is this region that varies in length. Within the 59-base element family, a few grou ...
Repair of Site-Specific DNA Double-Strand Breaks in
... DSB repair by NHEJ is usually accompanied by loss or gain (or loss and gain) of nucleotides. Therefore, we evaluated the efficiency of DSB repair via NHEJ by testing for short deletions (<30 bp; often linked with classical NHEJ) and longer deletions (indicating alternative end joining; Deriano and Ro ...
... DSB repair by NHEJ is usually accompanied by loss or gain (or loss and gain) of nucleotides. Therefore, we evaluated the efficiency of DSB repair via NHEJ by testing for short deletions (<30 bp; often linked with classical NHEJ) and longer deletions (indicating alternative end joining; Deriano and Ro ...
Test Info Sheet
... Aniridia is a developmental anomaly of the entire eye, characterized by varying degrees of iris hypoplasia. Ocular abnormalities associated with aniridia include persistent papillary membrane, congenital cataracts, ectopia lentis, developmental glaucoma, corneal pannus with progressive keratopathy a ...
... Aniridia is a developmental anomaly of the entire eye, characterized by varying degrees of iris hypoplasia. Ocular abnormalities associated with aniridia include persistent papillary membrane, congenital cataracts, ectopia lentis, developmental glaucoma, corneal pannus with progressive keratopathy a ...
SVD and PCA
... • Images under different illumination • Most variation captured by first 5 principal components – can re-illuminate by combining only a few images ...
... • Images under different illumination • Most variation captured by first 5 principal components – can re-illuminate by combining only a few images ...
greenland ancient dna - Arctic and Alpine Research Group
... due to DNA damage (17). Approximately 31% of the sequences from the John Evans Glacier silty sample were assigned to plant taxa that passed the authentication and identification criteria. These belong to the order Rosales, the family Salicaceae, and the genus Saxifraga (Table 1). This result is cons ...
... due to DNA damage (17). Approximately 31% of the sequences from the John Evans Glacier silty sample were assigned to plant taxa that passed the authentication and identification criteria. These belong to the order Rosales, the family Salicaceae, and the genus Saxifraga (Table 1). This result is cons ...
rpoB gene sequence-based characterization of emerging non
... mycobacterial infections. Cumulative experience has indicated that this molecular tool underestimates the diversity of this group and does not distinguish between all recognized mycobacterial taxa. In order to improve the recognition of emerging rapidly growing mycobacteria (RGM), rpoB gene sequenci ...
... mycobacterial infections. Cumulative experience has indicated that this molecular tool underestimates the diversity of this group and does not distinguish between all recognized mycobacterial taxa. In order to improve the recognition of emerging rapidly growing mycobacteria (RGM), rpoB gene sequenci ...
Are 100 enough? Inferring acanthomorph teleost phylogeny using
... taxonomic range relative to those markers. This facilitates the capture of homologous loci that are useful for both old and more recent divergences, a property shared with UCEs [22, 27]. One advantage to AHE, which we utilize here, is the ease of generating reliable alignments due to the paucity of ...
... taxonomic range relative to those markers. This facilitates the capture of homologous loci that are useful for both old and more recent divergences, a property shared with UCEs [22, 27]. One advantage to AHE, which we utilize here, is the ease of generating reliable alignments due to the paucity of ...
Presentation
... S7L3. Students will recognize how biological traits are passed on to successive generations. A.Explain the role of genes and chromosomes in the process of inheriting a specific trait. B. Compare and contrast that organisms reproduce asexually and sexually (bacteria, protists, fungi, plants, and an ...
... S7L3. Students will recognize how biological traits are passed on to successive generations. A.Explain the role of genes and chromosomes in the process of inheriting a specific trait. B. Compare and contrast that organisms reproduce asexually and sexually (bacteria, protists, fungi, plants, and an ...
Endogenous Retroviral Elements in Human DNA1
... Abstract Endogenous retroviruses and retroviral elements represent a substan tial component of vertebrate genomes. They are inherited as stable Mendelian genes and may be activated spontaneously or by physical or chemical agents. In the human genome various retroviral elements have been detected by ...
... Abstract Endogenous retroviruses and retroviral elements represent a substan tial component of vertebrate genomes. They are inherited as stable Mendelian genes and may be activated spontaneously or by physical or chemical agents. In the human genome various retroviral elements have been detected by ...