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I. Comparing genome sequences
I. Comparing genome sequences

touchlight
touchlight

... This  document  includes  statements  that  are,  or  may  be  deemed  to  be,  "forward-­‐looking  statements".  These  forward-­‐looking  statements  can   be   iden1fied   by   the   use   of   forward-­‐looking   terminology,   including   the ...
Use of DNA Technology:
Use of DNA Technology:

... • Method to identify an individual based upon their DNA • Used in: • Criminal investigations • Parentage Testing ...
Paper Plasmid activity - Liberty Union High School District
Paper Plasmid activity - Liberty Union High School District

... (This is a gene from a vertebrate not a bacterium, so it is linear not circular.) 4. The start and stop sequences for transcribing the Jellyfish GFP or Glo gene are highlighted. 5. These are needed to transcribe the gene properly when it is read. 6. The HindIII & EcoR1 restriction enzyme cutting sit ...
DNA and Genes - Biology at Mott
DNA and Genes - Biology at Mott

... Uracil, Cytosine, and Guanine. DNA is doublestranded; RNA is single-stranded. ...
Supplemental Figures
Supplemental Figures

... Supplemental Data Figure 2. Analytic sensitivity of COLD-PCR / HRM assays. Analytic sensitivity of (A) fast and (B) full COLD-PCR/HRM assays to detect mutations in codons 12/13 of KRAS gene was established by the analysis of serial dilutions of DNA extracted from KRAS mutant A549 cell line (harbours ...
Biology I - DNA/RNA Test Review
Biology I - DNA/RNA Test Review

... 26. DNA is the code for making proteins at the ribosome. Why is it important for the cell to make so many proteins? ...
Plant Transformation
Plant Transformation

... been transformed and it lights up • Green Fluorescent Protein - from jellyfish under lights and filter the transgenic plants will fluoresce • GUS - glucuronidase gene will convert added substrate to blue color. ...
Lecture7
Lecture7

... • In a given sequence (e.g., an ORF), compute frequency distribution of codons (64 element array): codon usage array • Codon usage array for coding sequences is different from that for non-coding sequences • If the codon usage array for an ORF is much more similar to that of coding sequences than to ...
EK 3.A.1: DNA, and in some cases rna, is the primary
EK 3.A.1: DNA, and in some cases rna, is the primary

... • RNA polymerase makes mRNA • mRNA is processed in eukaryotes • mRNA interacts with the ribosome to initiate translation • At the start codon • Nucleotides on mRNA is read in triplet codons ...
Mutation Worksheet
Mutation Worksheet

... 9. Write the complementary DNA strand. 10.Write the mRNA strand from the master DNA strand. 11.Write the Amino Acid sequence. 12.If the seventh nucleotide in the original master strand of DNA was changed from A to T, what would the resulting new mRNA be. 13.Write the new Amino Acid sequence that res ...
DNA lecture Notes
DNA lecture Notes

... make use of the genetic info stored in DNA? – They need to change that information into proteins, which are made up of amino acids – This is all dependent on the sequence of DNA subunits ...
Name Hr. _____ Macabobby`s DNA Fingerprinting Webquest Part I
Name Hr. _____ Macabobby`s DNA Fingerprinting Webquest Part I

... used to screen parents and fetuses for the presence of inherited abnormalities, such as Huntington’s disease or muscular dystrophy, so appropriate advice can be given and precautions taken as needed. Do you think knowing your child had a genetic disease prior to its birth would help you or cause you ...
File
File

... typing to determine whether your suspicion is correct? How would you go about using DNA technology to test your theory? 3. Should DNA evidence alone be sufficient to convict when there is no corroborative evidence? State your opinion and provide support for it. 4. Extra Credit: A woman reports being ...
DNAppt
DNAppt

... acids • Tertiary Structure: 3-D structure of protein (the way the amino acids roll up together, like a wad of string) ...
electroporation of a - The Steve Clough Lab
electroporation of a - The Steve Clough Lab

... 5. Apply a single 2.5kV electrical pulse (field strength of 12.5 kV/cm) by simultaneously pressing both red buttoms on face of gene pulser. Pulser will beep when finished. Time reading ideally will be above 9.3, but lower values may still be ok. Time will be lower the more salt (remember that DNA is ...
RNA interference - Creighton University
RNA interference - Creighton University

... genes (lin-41 and lin-28) which direct developmental progress • At that time, the so-called small temporal RNAs (stRNAs) were found to repress translational of the target mRNAs by interacting with complementary sites in their 3’ untranslated regions (UTRs) • It was later appreciated that the stRNAs ...
DNA – The Double Helix In 1952, Rosalind Franklin discovered that
DNA – The Double Helix In 1952, Rosalind Franklin discovered that

... bond with Thymine. Guanine will only bond with Cytosine. This means the amount of cytosine in a cell will always equal the amount of guanine and the amount of adenine will always equal the amount of thymine. These bases can occur in any order along a strand of DNA. ***Note that the bases attach to t ...
news and views feature
news and views feature

... eukaryotes — several of which are completed, with many others being explored — range from less than 3 million bp for an intracellular microsporidian to the 4 billion found in the human genome, and more. The genomes of eukaryotes are, however, often greatly inflated by the presence of considerable am ...
Cell Reproduction
Cell Reproduction

... A man has a discolored area on the back of his hand. The doctor has assured him it is a harmless body cell mutation. Explain why the mutation probably will not appear in his children. This is a body cell mutation. If the mutation had appeared in an egg or sperm, a child that developed from the sex c ...
DNA Sequencing
DNA Sequencing

... presence of polynucleotide kinase, which attaches P labeled to the 5’terminal The labeled DNA fragment is then divided into four aliquots, each of which is treated with a reagent which modifies a specific base 1. Aliquot A + dimethyl sulphate, which methylates guanine residue 2. Aliquot B + formic ...
Teacher: Kolleen Kopchak Grade 9
Teacher: Kolleen Kopchak Grade 9

... Response Modes: I do not have students that require response modes as all my students are mild to moderate SLD identifications. However, if I did, I would education myself more on what to look for and develop each level (Pre, Emerging, and Symbolic). I would also collaborate with their previous teac ...
ANALYSE OF THE MOLECULAR EVOLUTION OF THE ZOONOTIC
ANALYSE OF THE MOLECULAR EVOLUTION OF THE ZOONOTIC

... fragments of the genomic DNA from the strains to be analyzed were added to this: if this genomic DNA contained the same genes as were present in the array, they bound to them, which could be measured with biochemistry techniques. A microarray analysis allows one to see the differences in the gene co ...
Ch_ 19_2
Ch_ 19_2

... adjust levels of enzymes for synthesis & digestion ...
Gene Section MSH3 (mutS homolog 3 (E. coli)) in Oncology and Haematology
Gene Section MSH3 (mutS homolog 3 (E. coli)) in Oncology and Haematology

... intensities, with higher expression in testis and pancreas and lower in small intestine and colon. ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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