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Topic 2 & 3: Genetics Review
Topic 2 & 3: Genetics Review

... This 2nd step, after the unwinding and separation of strands, involves having the single strands act as templates for the new strands. Free nucleotides are present in large numbers around the replication fork. The bases of these nucleotides form hydrogen bonds with the bases of the parent strand. DN ...
Lecture 9
Lecture 9

... (frequency – 10-9 ) or 1 in 106 replicated genes (10-6 ) • Mutations usually occur randomly along a chromosome. – A low rate of spontaneous mutations is beneficial in providing the genetic diversity needed for evolution. ...
Lecture#23 - Cloning genes by complementation
Lecture#23 - Cloning genes by complementation

... 1. The isolation of genes proceeds via screening libraries for a gene of interest. 2. A clone containing a specific gene may be identified if it is able to complement a host mutation (single cell organisms). 3. Unfortunately, most genes in most organisms, especially eukaryotes, cannot be isolated by ...
DNA.ELECTROPHORESISREVIEW
DNA.ELECTROPHORESISREVIEW

... Draw a simple graph showing the growth curve of E.Coli. Label it! Why are there different types of media? What is the difference between broth and agar? Define electrophoresis. Explain how fragments move on a gel. What side of the gel box is DNA placed on? Why? Explain why DNA is called a double hel ...
Comp. Genomics
Comp. Genomics

... • Distinguishes between a target sequence – T and other informative sequences (Is) that may contain gaps • States correspond to sequence types in the target sequence ...
ESSAY 1: CONCEPTION
ESSAY 1: CONCEPTION

... proteins and send them around the body, but the particular combinations of proteins cannot be predicted. There are also many ‘switches’ within the DNA that can be switched on by external factors. These external factors can control which proteins are made or unmade and that changes the result of the ...
Virtual Mark Recapture Lab
Virtual Mark Recapture Lab

... 4. Which of the following represents the correct order of steps in DNA fingerprinting? a. DNA isolationrestriction enzyme digestiongel electrophoresis b. Restriction enzyme digestiongel electrophoresisDNA isolation c. DNA isolationgel electrophoresisrestriction enzyme digestion d. None of the ...
Genetic Engineering - Needham Public Schools
Genetic Engineering - Needham Public Schools

... Benefits of Recombinant Bacteria 1. Bacteria can make human insulin or human growth hormone. 1. Bacteria can be engineered to “eat” oil spills. ...
Chapter 12 Section 1
Chapter 12 Section 1

... 3 critical things genes do: ...
File
File

... DNA Fingerprinting Activity Introduction: DNA fingerprinting relies on the fact that the DNA code is universal for all living things and that there are differences between individuals within that code. Because human DNA is very similar to every other human’s DNA, DNA fingerprinting primarily focuses ...
Nerve activates contraction
Nerve activates contraction

... Gene families have evolved by duplication of ancestral genes • Most genes are present as a single copy per haploid set of chromosomes • Multigene families exist as a collection of identical or very similar genes (exceptions). • These likely evolved from a single ancestral gene. • The members of mul ...
File
File

... reproduction through DNA replication during the ​S phase​, or DNA ​s​ynthesis phase of its life cycle. - Replication begins at the ​ORI​, or origin of replication 1) ​Helicase​ unzips the dress ;) to reveal the ​antiparallel​ strands of DNA: one goes 3’ to 5’ while the other goes 5’ to 3’ a) since h ...
Nucleic Acids: An Introduction
Nucleic Acids: An Introduction

... • One strand of the DNA double helix is used as a template by the RNA polymerase to synthesize a messenger RNA (mRNA). • mRNA migrates from the nucleus to the cytoplasm. • mRNA goes through different types of maturation including one called splicing when the non-coding sequences are eliminated. • Th ...
Biotechnology Powerpoint
Biotechnology Powerpoint

... Recombinant DNA: A new DNA sequence created when the DNA of one organism is inserted into the DNA of another organism. This “new combination” of DNA is known as recombinant DNA. ...
Sequence - andreawise
Sequence - andreawise

... literature database called PUBMED).  You can search for similar sequences using the feature called BLAST (by inputting all or part of a DNA or amino acid sequence) and compare two or more sequences. ...
Gene Structure
Gene Structure

... in relative close proximity (200 bp) to the gene. Higher eukaryotic cells often utilize DNA regulatory elements that can be located far away from the genes, either upstream or downstream, or even within introns of genes. ...
1. Gene Mutations
1. Gene Mutations

... 3. Watson and Crick (1953): using all the research to date, discovered the structure for DNA: A DOUBLE HELIX (with sugar-phosphate backbones and bases on the inside held together by H bonds) ...
Chargaff`s Rule - SheltonTechnologyPortfolio
Chargaff`s Rule - SheltonTechnologyPortfolio

...  Transformation was stopped when DNA-destroying enzymes were used. ...
Genetic information determines structure
Genetic information determines structure

... Steps in protein synthesis: - begins at the 5’ end of mRNA - translation begins at first “start” codon (AUG) on mRNA - tRNA molecule with anticodon that matches codon of mRNA is placed in active site of ribosome - amino acid is bonded to amino acid chain and released from tRNA - proceeds one codon a ...
Results of Exam 1 - Pennsylvania State University
Results of Exam 1 - Pennsylvania State University

... • All DNA polymerases need a primer, i.e. a 3’-OH to which new nucleotides can be added. • Synthesis of the new strand oriented away from direction of fork movement: – Discontinuous – Okazaki fragments: short DNA fragments initially made on the lagging strand and then ligated together. ...
polymerase chain reaction
polymerase chain reaction

... So he took this 1-inch long fish and cut away about 20% of their two chambered hearts. The incisions through the abdomen were blotted to stop bleeding, the fish were returned to the water and 8 out of 10 survived the experiment. “They sort of hang out at the bottom of the tank” it was reported. But ...
DNA The Double Helix
DNA The Double Helix

... construct a house, the DNA "blueprint" tells the cell how to build the organism.   Yet, how can a heart be so different from a brain if all the cells contain the same instructions?   Although much work remains in genetics, it has become apparent that a cell has the ability to turn off most genes and ...
Recitation Section 7 Answer Key Molecular Biology—DNA as
Recitation Section 7 Answer Key Molecular Biology—DNA as

... isolated live SIII from the dead mouse. We now understand it to be because some genes (segments of DNA) that encode for formation of polysaccharide coat were transferred in the experiment. 7. Were all the bacteria of the recipient type transformed? Describe the probable process from coinfection to ...
Proteomics
Proteomics

... Based on the cloning and amplification of identified ORFs into homologous (ideally used for bacterial and yeast proteins) or sometimes heterologous systems (insect cells which result in post-translational modifications similar to mammalian cells). A fusion tag (short peptide or protein domain that i ...
gal
gal

... element can re-mobilize and exit a disrupted gene, and in many cases return the gene to its original state, – may transpose to a conjugative plasmids, or near Hfr integration sites for wide spread dispersal, ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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