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2014 Spring Seminar Speaker Series Xuegong Zhang, PhD
2014 Spring Seminar Speaker Series Xuegong Zhang, PhD

... human niches. The next-generation sequencing (NGS) technology has made large-scale study of metagenomes feasible, which opens a promising new way for understanding our “other self”: the microbiomes that live with us. Comparing and discriminating metagenome samples is a basic task on analyzing metage ...
Control of Gene Expression in Prokaryotes
Control of Gene Expression in Prokaryotes

... produce enzymes and other proteins required to metabolize a nutrient in its environment? Justify your answer with specific details from Model 1 or Model 2. ...
Molecular Biology Fourth Edition
Molecular Biology Fourth Edition

... Hershey & Chase investigated bacteriophage, virus particle by itself, a package of genes – This has no metabolic activity of its own – When virus infects a host cell, the cell begins to make viral proteins – Viral genes are replicated and newly made genes with viral protein assemble into virus parti ...
Who are you? This question can be answered many ways…
Who are you? This question can be answered many ways…

... substance of DNA in 1868, while researching the nucleus of fish sperm. He did not know it’s purpose. • Scientists predicted that DNA held the information of inheritance, but they weren’t sure how. • Using X-ray diffraction, Rosalind Franklin discovered the structure of DNA as a double helix in 1951. ...
Review Questions yeast lecture 18
Review Questions yeast lecture 18

... 1. What general minimum requirements does a plasmid have in order to be useful for us as a tool in molecular biology? (1) ...
2011 - Barley World
2011 - Barley World

... 1. Considering the case of “Roundup Ready” sugarbeet seed production in the Willamette Valley, which of the following gene flow mechanisms is the most likely? a. Sugar b. Prions c. Eggs d. Pollen 2. If there is gene flow between a homozygous diploid Roundup Ready plant and a homozygous non-Roundup R ...
1 From Lewontin, The Triple Helix IV. Directions in the Study of
1 From Lewontin, The Triple Helix IV. Directions in the Study of

... been three attempts to bring biological phenomena under the aegis of very general properties of systems that are changing in time. They are the Three C's: catastrophe theory, chaos theory, and complexity theory. All are attempts to show that extremely simple relationships in dynamical systems will l ...
DNA structure and replication power point
DNA structure and replication power point

... hereditary material because it was more complex than DNA • Proteins were composed of 20 different amino acids in long polypeptide chains ...
Chapter 10
Chapter 10

... Identification of DNA • mRNA sequence comes from specific regions of DNA (Genes) • mRNA sequence is used to make proteins and defines the physical/behavioral characteristics of the organism • Therefore we use mRNA to identify active regions of DNA • Use mRNA sequence and base pairing rules to identi ...
Supplementary Information (doc 83K)
Supplementary Information (doc 83K)

... (dddW-lacZ) and pBIO1947 (SPO0454-lacZ). These plasmids were transferred in separate triparental conjugational matings with E. coli containing the mobilising plasmid pRK2013 as the helper strain (Figurski & Helinski, 1979) and R. leguminosarum 3841 (Young et al., 2006) or J470 (R. pomeroyi rifampici ...
Mechanisms of Genetic exchange
Mechanisms of Genetic exchange

... virus. Viruses are non-cellular entities known to infect all types of cells; those infecting bacteria are called bacteriophages. Viruses vary considerably in form and exact composition, but typically have a genome composed of either DNA or RNA (not both) surrounded by a protein coat. Though in most ...
AP Biology
AP Biology

... students and scientists alike can compare known and unknown DNA sequences, establish common relationships between organisms, and look for similar protein structures in different organisms. All in a matter of seconds. This lab is an activity introducing you to using this amazing computer program. The ...
General Biology Program for Secondary
General Biology Program for Secondary

... Woodrow 8). At the molecular level, DNA resembles a twisted ladder; this structure is called the DNA double helix (Hermanson-Miller and Woodrow 9). This lab activity makes DNA visible for students. The DNA extracted by students is precipitated into a large enough mass to see (Hermanson-Miller and Wo ...
Deoxyribonucleic acid, DNA
Deoxyribonucleic acid, DNA

... Griffith called this process transformation because one strain of bacteria (the harmless strain) had changed permanently into another (the disease-causing strain). Griffith hypothesized that a factor must contain information that could change harmless bacteria into disease-causing ones. ...
for Genetic Testing
for Genetic Testing

... and the length of the entire repeat is from 0.1 to 1 Mb. Satellite DNA is clustered in centromeric regions and is rarely used in genetic testing. • Minisatellites: the repeated unit typically ranges from 20 to 70 bp, and the length of the entire repeat may reach 20kb.This is the class most often ref ...
U4Word
U4Word

... tubes, one with each antibiotic. e. Cells which grow in the presence of tetracycline and not in the presence of ampicillin contain the recombinant plasmid. f. If the target was a cDNA any such cell (as in e) is used since the target is homogeneous, not a mixture of various DNAs. 2. A variation on in ...
Chapter 21
Chapter 21

... • The basis of change at the genomic level is mutation, which underlies much of genome evolution • The earliest forms of life likely had a minimal number of genes, including only those necessary for survival and reproduction • The size of genomes has increased over evolutionary time, with the extra ...
Milestone1
Milestone1

... 3) When trying to determine whether two genes are orthologous, one must consider the possibility that two different genes are similar because, over time, their sequences converged towards one another instead of sharing similarity because they diverged from a common ancestral gene. If two genes have ...
Sample Exam 3 answer key
Sample Exam 3 answer key

... Plant B: Two independent insertions of the T-DNA occurred on different chromosomes. Therefore the two T-DNA insertions are unlinked. When plant B is selfed, the two loci will segregate 9:3:3:1, with 15 individuals that are kanamycin resistant and 1 individual out of 16 that is kanamycin sensistive. ...
File
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... combination of mutations in a single cell allows uncontrolled cell division. • Further mutations expand the tumor cells' ability to divide and spread. • Cancer is most likely to strike older people, those who have been exposed to mutationcausing agents, and those who have inherited mutations in canc ...
Genetica per Scienze Naturali aa 05
Genetica per Scienze Naturali aa 05

... because not enough time has elapsed since the last common ancestor for large numbers of changes to have occurred. In contrast, because of functional constraints in human-mouse comparisons the exons in genes stand out as small islands of conservation in a sea of introns. The sequence conservation fou ...
Biology 120 Lab Exam 2 Review Session
Biology 120 Lab Exam 2 Review Session

Product Datasheets
Product Datasheets

... hours to overnight) and reaction volume is recommend. ➢ Preparation of DNA insert fragment by PCR or chemical synthesis ✔ If DNA fragments are prepared by PCR, purify the DNA fragments through a PCR cleanup kit. After preparing your DNA fragments by PCR, verify the PCR products by gel electrophoresi ...
Slide 1
Slide 1

... Replication – the process DNA uses to copy itself Bacteriophage – a virus that infects bacteria Virus – a type of germ that is not living Marker – a material, such as an atom, used to mark an item • Semi-conservative replication – a model of DNA replication in which an old strand of DNA is used to m ...
Project 1 Concepts in Biology Project 1 Development of a PCR
Project 1 Concepts in Biology Project 1 Development of a PCR

... Mutations and Disease DNA is constantly subject to mutations, accidental changes in its code. Mutations can lead to missing or malformed proteins, and that can lead to disease. We all start out our lives with some mutations. These mutations inherited from your parents are called germ-line mutations. ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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