Characterisation of the katA gene encoding a catalase and evidence
... 20-kb EcoRI fragment is detected in the wild-type, which is substantially shortened in the TX350 DNA due to the introduction of an EcoRI restriction site within the ermB cassette (data not shown). In addition to the katA-containing band, a second weaker hybridisation signal of about 4.5 kb was detec ...
... 20-kb EcoRI fragment is detected in the wild-type, which is substantially shortened in the TX350 DNA due to the introduction of an EcoRI restriction site within the ermB cassette (data not shown). In addition to the katA-containing band, a second weaker hybridisation signal of about 4.5 kb was detec ...
exploring genetics - Cold Spring Harbor Laboratory
... Standard Course of Study for the middle school grades. The career information, reflection activities, and the inquiry based nature of the activities provide a well rounded introduction to genetic science and its relation to mathematics at the middle grades level. The kit was developed as an outreach ...
... Standard Course of Study for the middle school grades. The career information, reflection activities, and the inquiry based nature of the activities provide a well rounded introduction to genetic science and its relation to mathematics at the middle grades level. The kit was developed as an outreach ...
Point Mutation in Intron Sequence Causes Altered Carboxyl
... exceptionally wide differences in susceptibility also within species. Two of these, the divergence between inbred C57BL/6 and DBA/2 mice on the one hand and that between inbred L-E (Turku AB) and outbred H/W (Kuopio) rats on the other, have been exhaustively characterized and exploited as biological ...
... exceptionally wide differences in susceptibility also within species. Two of these, the divergence between inbred C57BL/6 and DBA/2 mice on the one hand and that between inbred L-E (Turku AB) and outbred H/W (Kuopio) rats on the other, have been exhaustively characterized and exploited as biological ...
DNA MUTATIONS OF THE CAT The good, the bad and the ugly
... The word ‘mutation’ generally conjures negative thoughts and associations, such as Frankenstein’s monster or the X-Men. However, just as Frankenstein’s monster was misunderstood, and the X-Men can use their powers for good or evil, so too are the dNA mutations found in the mammalian genome, includin ...
... The word ‘mutation’ generally conjures negative thoughts and associations, such as Frankenstein’s monster or the X-Men. However, just as Frankenstein’s monster was misunderstood, and the X-Men can use their powers for good or evil, so too are the dNA mutations found in the mammalian genome, includin ...
Gene Technology Regulation 2002
... Notifiable low risk dealings suitable for at least physical containment level 2 or 3 ...
... Notifiable low risk dealings suitable for at least physical containment level 2 or 3 ...
DNA Sequencing (cont.) - A computational tour of the human genome
... • Remove transitively inferable overlaps If read r overlaps to the right reads r1, r2, and r1 overlaps r2, then (r, r2) can be inferred by (r, r1) and (r1, r2) ...
... • Remove transitively inferable overlaps If read r overlaps to the right reads r1, r2, and r1 overlaps r2, then (r, r2) can be inferred by (r, r1) and (r1, r2) ...
Exome sequencing as a tool for Mendelian disease gene discovery
... nonsense single-base substitutions or small insertion– deletions (that is, indels), are predicted to have functional consequences and/or to be deleterious14. As such, the exome represents a highly enriched subset of the genome in which to search for variants with large effect sizes. Defining the exo ...
... nonsense single-base substitutions or small insertion– deletions (that is, indels), are predicted to have functional consequences and/or to be deleterious14. As such, the exome represents a highly enriched subset of the genome in which to search for variants with large effect sizes. Defining the exo ...
Guppies – Quang Anh
... coloration (mostly bright) that the simulation starts with and also the end result (gene frequency of the bright guppies is 0.1). This is one of the main factors that make the hypothesis testable as it has specific values that any reader can apply on the simulation. Another reason is because it has ...
... coloration (mostly bright) that the simulation starts with and also the end result (gene frequency of the bright guppies is 0.1). This is one of the main factors that make the hypothesis testable as it has specific values that any reader can apply on the simulation. Another reason is because it has ...
Discovery and characterization of chromatin states for Please share
... that compactly and adequately describes the biological datasets (Online Methods), based on a two-stage nested initialization procedure. In the first stage, we learn a large compendium of models at varying numbers of states and from multiple random initializations, and select a best scoring model (Su ...
... that compactly and adequately describes the biological datasets (Online Methods), based on a two-stage nested initialization procedure. In the first stage, we learn a large compendium of models at varying numbers of states and from multiple random initializations, and select a best scoring model (Su ...
Title: Statistical Evidence for Common Ancestry
... 1. The common ancestry of life is supported by an enormous body of evidence and is universally accepted within the scientific community. However, some potential sources of data that can be used to test the thesis of common ancestry have not yet been formally analyzed. 2. We developed a new test of c ...
... 1. The common ancestry of life is supported by an enormous body of evidence and is universally accepted within the scientific community. However, some potential sources of data that can be used to test the thesis of common ancestry have not yet been formally analyzed. 2. We developed a new test of c ...
Homologous recombination in budding yeast expressing the human
... Assaying DSB-stimulated ectopic gene conversion (EGC) Single colonies of strains of the appropriate genotype that contained the his3-Δ3 -HOcs allele at the HIS3 locus on chromosome XV, and the his3-ΔMscI allele at the LEU2 locus on chromosome III were used to inoculate a minimum of 10 one, or five ...
... Assaying DSB-stimulated ectopic gene conversion (EGC) Single colonies of strains of the appropriate genotype that contained the his3-Δ3 -HOcs allele at the HIS3 locus on chromosome XV, and the his3-ΔMscI allele at the LEU2 locus on chromosome III were used to inoculate a minimum of 10 one, or five ...
Ectopic overexpression of castor bean LEAFY
... Sequence-based quantitative trait loci (QTL) analysis has associated many rice and maize genes that encode products containing B3 domain genes with traits such as grain yield, seed weight and number, and protein content [29]. However, the proposed associations of B3-domain-containing master regulato ...
... Sequence-based quantitative trait loci (QTL) analysis has associated many rice and maize genes that encode products containing B3 domain genes with traits such as grain yield, seed weight and number, and protein content [29]. However, the proposed associations of B3-domain-containing master regulato ...
this PDF file - E-Journal Faculty of Medicine Universitas
... this statement, such as studies on CP-related genes as well as studies on the presence of single nucleotide polymorphisms (SNPs).4,5 In this respect, Callaghan et al.6 had conducted a systematic literature review and discovered that there were approximately 22 studies related to the relationship of ...
... this statement, such as studies on CP-related genes as well as studies on the presence of single nucleotide polymorphisms (SNPs).4,5 In this respect, Callaghan et al.6 had conducted a systematic literature review and discovered that there were approximately 22 studies related to the relationship of ...
Complete
... In microfluidic approaches for manipulating biological molecules, the conventional test tubes and pipettes are replaced by miniaturized plumbing channels (with 0.1- to 100µm dimensions) etched into the surface of a wafer using methods borrowed from the integrated circuit industry, such as photolitho ...
... In microfluidic approaches for manipulating biological molecules, the conventional test tubes and pipettes are replaced by miniaturized plumbing channels (with 0.1- to 100µm dimensions) etched into the surface of a wafer using methods borrowed from the integrated circuit industry, such as photolitho ...
Switching between polymerase and exonuclease sites in DNA
... the catalytic domain of Pol2 (Pol2core (aa 1–1228)) revealed that Pol ε lacks the extended -hairpin loop found in most other B-family DNA polymerases that has been proposed to interact with the template DNA and to function as a wedge by breaking hydrogen bonds between the template and nascent stran ...
... the catalytic domain of Pol2 (Pol2core (aa 1–1228)) revealed that Pol ε lacks the extended -hairpin loop found in most other B-family DNA polymerases that has been proposed to interact with the template DNA and to function as a wedge by breaking hydrogen bonds between the template and nascent stran ...
Epigenetic Regulation ofbdnfGene Transcription in the
... hippocampal tissue, purified, and processed for bisulfite modification. For all our methylation-specific real-time PCR (MSP) data, we performed bisulfite treatment of genomic DNA an average of 5– 6 times. Bisulfite treatment of genomic DNA converts cytosine to uracil, but leaves methylated 5⬘-cytosi ...
... hippocampal tissue, purified, and processed for bisulfite modification. For all our methylation-specific real-time PCR (MSP) data, we performed bisulfite treatment of genomic DNA an average of 5– 6 times. Bisulfite treatment of genomic DNA converts cytosine to uracil, but leaves methylated 5⬘-cytosi ...
The Coat of Many Colors
... inserted into the merle gene contains a long string of “A’s” that “turn off” the SILV gene so that it cannot produce the SILV protein helping to form melanosomes. It turns out that mutations occur in this string of “A’s”quite often as cells divide, decreasing the string’s length. The result is that ...
... inserted into the merle gene contains a long string of “A’s” that “turn off” the SILV gene so that it cannot produce the SILV protein helping to form melanosomes. It turns out that mutations occur in this string of “A’s”quite often as cells divide, decreasing the string’s length. The result is that ...
Visualization, description and analysis of the Drosophila melanogaster
... A mutation is an adaptively non-directed change in the genomic sequence of an individual, and mutations in the DNA molecule are the ultimate source of genetic variation. Once a new variant appears by mutation in the DNA it can be replicated and transmitted from generation to generation. Gel electrop ...
... A mutation is an adaptively non-directed change in the genomic sequence of an individual, and mutations in the DNA molecule are the ultimate source of genetic variation. Once a new variant appears by mutation in the DNA it can be replicated and transmitted from generation to generation. Gel electrop ...
Inferring Host Gene Subnetworks Involved in Viral
... unknown (G, H), and two genes whose phenotypes are weak (D, L), are actually key host factors involved in viral replication. The integer linear program used in our approach consists of an objective function and a set of constraints characterizing subnetworks that are deemed biologically interpretabl ...
... unknown (G, H), and two genes whose phenotypes are weak (D, L), are actually key host factors involved in viral replication. The integer linear program used in our approach consists of an objective function and a set of constraints characterizing subnetworks that are deemed biologically interpretabl ...
Environmental Microbiology
... opa (opacity) genes in Neisseria meningitides and Neisseria gonorhoeae species, switching loci ON and OFF is based on changes in the number of pentameric repeat elements (5′-CTTCT-3′) with which the expression state of the opa gene(s) changes. For example, 6, 9 or 12 repeats are equivalent to an Opa ...
... opa (opacity) genes in Neisseria meningitides and Neisseria gonorhoeae species, switching loci ON and OFF is based on changes in the number of pentameric repeat elements (5′-CTTCT-3′) with which the expression state of the opa gene(s) changes. For example, 6, 9 or 12 repeats are equivalent to an Opa ...
Non-invasive Prenatal Testing for Chromosomal Abnormality using
... dichorionic, and so may be discordant, maternal plasma DNA testing would, in theory, not be as straightforward. Two studies reporting relatively small series of twin pregnancies have suggested that shotgun sequencing-based approaches are able to detect fetal trisomies in twin pregnancies.52,53 It is ...
... dichorionic, and so may be discordant, maternal plasma DNA testing would, in theory, not be as straightforward. Two studies reporting relatively small series of twin pregnancies have suggested that shotgun sequencing-based approaches are able to detect fetal trisomies in twin pregnancies.52,53 It is ...
Spectrum of Mutations in MMAB Identified by
... of L-methylmalonyl-CoA to succinyl-CoA. Somatic cells studies have been used to evaluate patient samples for cobalamin related disorders. Due to high basal levels of propionate incorporation, some patients with mild MMA biochemical phenotypes cannot be diagnosed by complementation analysis. A high r ...
... of L-methylmalonyl-CoA to succinyl-CoA. Somatic cells studies have been used to evaluate patient samples for cobalamin related disorders. Due to high basal levels of propionate incorporation, some patients with mild MMA biochemical phenotypes cannot be diagnosed by complementation analysis. A high r ...