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Centromere Locations and Associated Chromosome
Centromere Locations and Associated Chromosome

... CEN5 is more complex. The formation of chromosome V of A. thaliana involved a reciprocal translocation (see above and Figure 1), and an understanding of this event is critical for interpreting the origin of CEN5. Previous comparative molecular cytogenetic analyses of A. thaliana and A. lyrata could ...
Complete genome sequence of the thermophilic Thermus sp
Complete genome sequence of the thermophilic Thermus sp

... isomerases), pulp and paper manufacturing (e.g. xylanases) as well as animal feed and human food production (amino acid and vitamin synthesis) [13, 14]. Here, we present a summary of classification and a set of features for Thermus sp. CCB_US3_UF1, together with the description of the complete genom ...
Exploring Genetics Across the Middle School Science
Exploring Genetics Across the Middle School Science

... team of university scientists, middle grades classroom teachers, middle school students developed and tested the activities in a school setting. The activities are easily implemented and follow current Common Core Math standards, the North Carolina Essential Science Standards and the former North Ca ...
Compaction of Duplex Nucleic Acids upon Native
Compaction of Duplex Nucleic Acids upon Native

... To find out which tridimensional structures are compatible with the experimental CCS values of the 12bp5- duplexes, we first carried out unbiased MD simulations directly from B- and A-helix structures, stripped of the solvent. Figure 1B,C shows representative CCS distributions. All such simulations ...
Supplemental Tables
Supplemental Tables

... also posted at the AAA site, were also used in this analysis. The FlyBase inferred cytological map locations were assigned to all of the orthologs called in the four species. These associations were then ordered and sorted according to their scaffold assignments and molecular coordinates for each sp ...
fulltext - DiVA portal
fulltext - DiVA portal

... only cytolytic factor found in non-pathogenic strains of E. coli including the K-12 strains commonly used in laboratory studies [2-7]. The clyA+ transcription is known to be subjected to transcriptional silencing by the H-NS nucleoid protein in E. coli K-12 [8] and it can be activated by the transcr ...
T-box-mediated control of the anabolic proline biosynthetic genes of
T-box-mediated control of the anabolic proline biosynthetic genes of

... Bacillus subtilis possesses interlinked routes for the synthesis of proline. The ProJ–ProA–ProH route is responsible for the production of proline as an osmoprotectant, and the ProB–ProA–ProI route provides proline for protein synthesis. We show here that the transcription of the anabolic proBA and ...
Genomics Reveal Ancient Forms of Stanniocalcin in Amphioxus and
Genomics Reveal Ancient Forms of Stanniocalcin in Amphioxus and

... Synopsis Stanniocalcin (STC) is present throughout vertebrates, including humans, but a structure for STC has not been identified in animals that evolved before bony fish. The origin of this pleiotropic hormone known to regulate calcium is not clear. In the present study, we have cloned three stanni ...
High-resolution melting analysis of the single nucleotide
High-resolution melting analysis of the single nucleotide

... Clostridium difficile, a Gram-positive, spore-forming, anaerobic bacterium, is the main causative agent of hospital-acquired diarrhoea worldwide. In addition to metronidazole and vancomycin, rifaximin, a rifamycin derivative, is a promising antibiotic for the treatment of recurring C. difficile infe ...
Gene Section MLL (myeloid/lymphoid or
Gene Section MLL (myeloid/lymphoid or

... t(11;19)(q23;p13)/ANLL --> MLL/EEN ...
Phylogenomics of type II DNA topoisomerases
Phylogenomics of type II DNA topoisomerases

... 1,2). In addition, specific DNA topoisomerases can introduce supercoils in closed circular DNA duplexes, determining both DNA secondary and tertiary structures via changes in the twist and the writhing of DNA duplexes. In order to modify the number of topological links (linking number, Lk) between t ...
Testing Services Markets, SNP Analysis, DNA Sequencing, Retail Clinics, Companion Diagnostics Brochure
Testing Services Markets, SNP Analysis, DNA Sequencing, Retail Clinics, Companion Diagnostics Brochure

... Are companies seeking profit, or a 'back-door' around FDA regulations? And does it have growth potential as a strategy. DNA sequencing and SNPs are essential for genetic testing, clearly the future of diagnostics. What companies/products are dominating these areas, and what growth can be expected? R ...
Alu repeat analysis in the complete human genome: trends and
Alu repeat analysis in the complete human genome: trends and

... has very low densities of Alu S and J, in fact, least density of Alu S in human genome. Similar trend was observed in chromosomes 13 and 9, with chromosome 13 having least density of Alu J subfamily (Supplementary material II). On the other hand, Chromosomes 8 and X were richer in Alu S and J subfam ...
Application Note: Targeted sequencing and chromosomal haplotype
Application Note: Targeted sequencing and chromosomal haplotype

... using just one primer pair complementary to a short locusspecific sequence. TLA is a strategy to selectively amplify complete loci on the basis of crosslinking physically proximal sequences. Unlike other targeted sequencing methods, TLA works without prior detailed locus information, as one primer p ...
THE SYNTHESIS OF DNA AND NUCLEAR PROTEIN BY
THE SYNTHESIS OF DNA AND NUCLEAR PROTEIN BY

... of De Tomasi as described by Pearse (1960), were used for the determination of the DNA content of germ and Leydig cell nuclei. The microspectrophotometer and general methods used for these determinations have been described by Cleland (1965). The two-wavelength method was adopted, the wavelengths us ...
Cross-talk between cell-cycle control and the environment
Cross-talk between cell-cycle control and the environment

... Even though the understanding of cell-cycle regulators in plants has tremendously increased over the last years, still little is known about cell-cycle regulation in response to environmental signals like DNA damage. A ubiquitous stress for any organism is DNA stress that can either be caused by exo ...
Microsoft Word (Chapter 3) - DORAS
Microsoft Word (Chapter 3) - DORAS

... Region II is typically located in the C-terminus of outer membrane receptors and has the consensus sequence (F/I/L/M/V)XXX(I/L/V)XNLX(D/N)(K/R)XY. The amino acid sequence of the mature FhuA1 was analysed and found to contain the motif ASLNVANLFDKEY at position 596 - 609, which shows good homology to ...
IMPROVE SMALL RNA-MEDIATED GENE SILENCING
IMPROVE SMALL RNA-MEDIATED GENE SILENCING

... We now have a much greater understanding of the gene silencing pathways in plants and their crucial roles in a variety of biological regulation processes, such as development, plant defense against invading viral nucleic acids, and epigenetic modifications. siRNA directed target repression can occur ...
Sporadic Hypertrophic Cardiomyopathy Due to De Novo Myosin
Sporadic Hypertrophic Cardiomyopathy Due to De Novo Myosin

... with familial hypertrophic cardiomyopathy frequently have multiple affected relatives, and must have one affected parent. Sporadic occurrence of hypertrophic cardiomyopathy is defined by similar anatomical and clinical features in an individual whose parents do not have the condition (6, 15). Estima ...
the evolution of genomic base composition in bacteria
the evolution of genomic base composition in bacteria

... the mutational bias toward GC and varies between zero and one. At steady state, the expected GC content under this model is equal to g. If the mutational bias, g, remained constant over time, it would be essentially impossible to explain the enormous variation in GC content observed among bacteria. ...
Polygenes and Modifier Genes for Tetracycline and
Polygenes and Modifier Genes for Tetracycline and

... ‘double dose effect’ does not occur. The tet in 6T20, MT93 and TM14 DNA preparations has additive effects with the mtr in 4B10, increasing its level of resistance from 0.3 to 0.6 ,ug ml-l. The same effect occurs using mtr DNA (4B10 or MT93) in combination with tet (6T20). The tet tern in TM14 DNA ha ...
ARTICLE IN PRESS
ARTICLE IN PRESS

... subcultured to obtain pure cultures, after which they were routinely grown in the appropriate broth media. LAB isolates were grown in MRS broth, while enterobacterial isolates were grown in nutrient broth using the incubation conditions described. All pure cultures were examined using light microsco ...
During the infection of macrophages by bacteria - HAL
During the infection of macrophages by bacteria - HAL

... allowing appropriate DNA repair through homologous recombination or non-homologous end joining [1]. Recently, H2AX has been used in several clinical studies as a very sensitive molecular marker of DNA damage and repair in cancer [4, 5]. Some bacteria exhibit genotoxic potential. For instance, Nouga ...
View Tutorial
View Tutorial

... and accumulate over evolutionary time. As a result, the same genes compared across multiple species will not be identical, but will display insertions, deletions, or substitutions of nucleotides. Since DNA codes for protein, similar changes occur in protein sequences. Over long periods of time, subs ...
biotechnology 22b
biotechnology 22b

... DNA chain cleanly at any point. • The enzyme selected will determine where the chain is cut. • Then two desirable parts can be spliced back together. • This produces an organism with a new set of traits. The process is often called gene splicing. ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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