Identification of novel epigenetic biomarkers in colorectal cancer
... with an incidence of 3500 new cases per year in Norway alone. There is a need for improved early diagnostics as well as more precise cancer diagnosis to better guide the choice of treatment. CpG island hypermethylation of tumour-suppressor genes has been established as a key molecular event in color ...
... with an incidence of 3500 new cases per year in Norway alone. There is a need for improved early diagnostics as well as more precise cancer diagnosis to better guide the choice of treatment. CpG island hypermethylation of tumour-suppressor genes has been established as a key molecular event in color ...
Evaluation of the Water Stress-Inducible
... Water deficit-inducible promoters that function in multiple species are valuable components for engineering stress-tolerant crops. Wsi18 is a water deficit-inducible promoter native to Oryza sativa. In this study, Brachypodium distachyon (B. distachyon) was used to determine if Wsi18 retained its wa ...
... Water deficit-inducible promoters that function in multiple species are valuable components for engineering stress-tolerant crops. Wsi18 is a water deficit-inducible promoter native to Oryza sativa. In this study, Brachypodium distachyon (B. distachyon) was used to determine if Wsi18 retained its wa ...
A Single Eubacterial Origin of Eukaryotic
... 2, the eukaryote sequences were not monophyletic, suggesting that shared compositional bias is not responsible for bringing these sequences together in subsequent phylogenetic analyses. Because we observed moderate base composition heterogeneity at codon positions 1 and 2, we used LogDet/Paralinear ...
... 2, the eukaryote sequences were not monophyletic, suggesting that shared compositional bias is not responsible for bringing these sequences together in subsequent phylogenetic analyses. Because we observed moderate base composition heterogeneity at codon positions 1 and 2, we used LogDet/Paralinear ...
Regulation of Bacterial Conjugation in Microaerobiosis by
... host-encoded regulators may control conjugal transfer in most (perhaps all) F-like plasmids while others may be plasmid specific. For instance, F transfer undergoes a drastic decrease in stationary phase (Frost and Manchak 1998), a behavior that is not observed in pSLT (Camacho et al. 2005b). Anothe ...
... host-encoded regulators may control conjugal transfer in most (perhaps all) F-like plasmids while others may be plasmid specific. For instance, F transfer undergoes a drastic decrease in stationary phase (Frost and Manchak 1998), a behavior that is not observed in pSLT (Camacho et al. 2005b). Anothe ...
Methods for detecting positive selection and examples among fungi
... positive selection, using either divergence (i.e., variation representing substitutions between species) or polymorphism data. We then illustrate applications of such methods, using relevant studies where selected genes have been identified in pathogens, providing insight into host–pathogen coevoluti ...
... positive selection, using either divergence (i.e., variation representing substitutions between species) or polymorphism data. We then illustrate applications of such methods, using relevant studies where selected genes have been identified in pathogens, providing insight into host–pathogen coevoluti ...
How Cations Can Assist DNase I in DNA Binding and Hydrolysis
... Sites I and II in bpDNase I, identical to their X-ray counterparts, were very strong cation-binding pockets, always occupied by ions throughout the trajectories (Table 3). Na+ spontaneously occupied these sites in the absence of Ca2+ (Sim1). Ion coordination was optimal for both Na+ and Ca2+ (Table ...
... Sites I and II in bpDNase I, identical to their X-ray counterparts, were very strong cation-binding pockets, always occupied by ions throughout the trajectories (Table 3). Na+ spontaneously occupied these sites in the absence of Ca2+ (Sim1). Ion coordination was optimal for both Na+ and Ca2+ (Table ...
File - Jaidean St Onge
... She died due to heart failure in 1931 where she was buried in Stockholm. Herman Goring had Carin’s remains moved three years later to his residence outside of Berlin, Karinhall. Eventually after the war, Karinhall was destroyed and no one knew what had happened to Carin’s remains. In 1951 some skele ...
... She died due to heart failure in 1931 where she was buried in Stockholm. Herman Goring had Carin’s remains moved three years later to his residence outside of Berlin, Karinhall. Eventually after the war, Karinhall was destroyed and no one knew what had happened to Carin’s remains. In 1951 some skele ...
Proviral amplification of the Gypsy endogenous retrovirus of
... (A) Organization of the gypsy element and the Act-env construct. Numbers refer to gypsy nucleotides (Marlor et al., 1986). Boxes correspond to ORFs. Two long terminal repeats (LTRs), shown by right-pointing arrowheads, flank a central region which contains the three ORFs corresponding to gag, pol an ...
... (A) Organization of the gypsy element and the Act-env construct. Numbers refer to gypsy nucleotides (Marlor et al., 1986). Boxes correspond to ORFs. Two long terminal repeats (LTRs), shown by right-pointing arrowheads, flank a central region which contains the three ORFs corresponding to gag, pol an ...
Gene conversion rapidly generates major histocompatibility complex
... haplotypes at the mitochondrial control region and cytochrome b genes respectively, across all 13 populations (Illera et al. 2007). The pipit was also found to have lower levels of genetic variation compared to other passerine species at a set of conserved microsatellite markers (Dawson et al. 2010) ...
... haplotypes at the mitochondrial control region and cytochrome b genes respectively, across all 13 populations (Illera et al. 2007). The pipit was also found to have lower levels of genetic variation compared to other passerine species at a set of conserved microsatellite markers (Dawson et al. 2010) ...
Identification of New Genes Involved in Meiosis by a Genetic Screen
... Budding yeast Saccharomyces cerevisiae contains a group of proteins named ZMM that constitutes a link between recombination and Synaptonemal Complex (SC) assembly. Yeast mutants that lack ZMM proteins have defects in recombination, SC formation and nuclear division progression. Meiotic cell cycle pr ...
... Budding yeast Saccharomyces cerevisiae contains a group of proteins named ZMM that constitutes a link between recombination and Synaptonemal Complex (SC) assembly. Yeast mutants that lack ZMM proteins have defects in recombination, SC formation and nuclear division progression. Meiotic cell cycle pr ...
Natural rules for Arabidopsis thaliana
... the complicacy of plant species, there are many uncertainties of plant pre-mRNA splicing mechanisms [12]. The signals that specifically define the borders of splicing sites in plant are still not fully understood due to lack of a complete and accurate description of the gene structure on the basis o ...
... the complicacy of plant species, there are many uncertainties of plant pre-mRNA splicing mechanisms [12]. The signals that specifically define the borders of splicing sites in plant are still not fully understood due to lack of a complete and accurate description of the gene structure on the basis o ...
Laboratory manual for the diagnosis of whooping cough caused by... pertussis/ Bordetella parapertussis
... BvgA/S genes, the vag are not expressed. Instead, a set of other genes called vir-repressed genes (vrg) are expressed. The function of the proteins encoded by the vrg and their role in the pathogenicity of B. pertussis are not yet known. In that case Bordetella species are called phase IV Bordetella ...
... BvgA/S genes, the vag are not expressed. Instead, a set of other genes called vir-repressed genes (vrg) are expressed. The function of the proteins encoded by the vrg and their role in the pathogenicity of B. pertussis are not yet known. In that case Bordetella species are called phase IV Bordetella ...
Poly ADP-ribosylation: a histone shuttle mechanism in DNA excision
... In DNA excision repair of mammalian cells, the tight association of histones with DNA is locally disrupted. Newly synthesized repair patches appear transiently in DNA regions which are accessible to chemical and enzymatic probes. In this process, nucleosomes are unfolded concomitant with incision (S ...
... In DNA excision repair of mammalian cells, the tight association of histones with DNA is locally disrupted. Newly synthesized repair patches appear transiently in DNA regions which are accessible to chemical and enzymatic probes. In this process, nucleosomes are unfolded concomitant with incision (S ...
Two novel heterozygous mutations of EVC2 cause a mild phenotype
... producing a truncated protein containing 241 amino acids. Given these possibilities, exon 6 skipping appears to be the most likely form of alternative splicing. A considerable number of EvC cases have already been screened for mutations, including the systematic screening of 65 EvC cases in which al ...
... producing a truncated protein containing 241 amino acids. Given these possibilities, exon 6 skipping appears to be the most likely form of alternative splicing. A considerable number of EvC cases have already been screened for mutations, including the systematic screening of 65 EvC cases in which al ...
Bacterial plasmids - Micro-Rao
... Mobilizable plasmids are those plasmids that lack genes to initiate self transfer but do encode the functions needed specifically for transfer of their own DNA. The initiation function is provided by other conjugative plasmid present in the same cell. Suicide plasmids are referred to those plasmids ...
... Mobilizable plasmids are those plasmids that lack genes to initiate self transfer but do encode the functions needed specifically for transfer of their own DNA. The initiation function is provided by other conjugative plasmid present in the same cell. Suicide plasmids are referred to those plasmids ...
Binding of Hoechst with nucleic acids using fluorescence spectroscopy
... with the double helix not such strong as with single chains or hairpin structures. The life-time of Hoechst at binding with DNA was increased from 0.4 ns to only 3 ns. Furthermore, there was a shift (albeit it was small) to shorter wavelengths of the emission spectrum. This means that Hoechst not on ...
... with the double helix not such strong as with single chains or hairpin structures. The life-time of Hoechst at binding with DNA was increased from 0.4 ns to only 3 ns. Furthermore, there was a shift (albeit it was small) to shorter wavelengths of the emission spectrum. This means that Hoechst not on ...
DNA methylation profiling identifies epigenetic dysregulation in
... methylation status of 27 578 CpG sites corresponding to 14 475 consensus coding sequences and well-known cancer genes (Bibikova et al, 2009). Although the Infinium methylation assay is not a genome-wide DNA methylation technology, it is a useful screening tool that is sensitive, specific and highly re ...
... methylation status of 27 578 CpG sites corresponding to 14 475 consensus coding sequences and well-known cancer genes (Bibikova et al, 2009). Although the Infinium methylation assay is not a genome-wide DNA methylation technology, it is a useful screening tool that is sensitive, specific and highly re ...
Highly sensitive DNA sensor based on upconversion nanoparticles
... provoked by the absorption of other species20 limit the detection sensitivity. These drawbacks could be overcome using upconversion nanoparticles (UCNPs) as fluorescence donors21. The UCNPs are lanthanide doped inorganic materials, which are able to absorb two or more low energy photons and emit flu ...
... provoked by the absorption of other species20 limit the detection sensitivity. These drawbacks could be overcome using upconversion nanoparticles (UCNPs) as fluorescence donors21. The UCNPs are lanthanide doped inorganic materials, which are able to absorb two or more low energy photons and emit flu ...
Analysis of single nucleotide polymorphisms in human
... of amino acid (nonsynonymous) are lower or about the same as substitutions which result in silent changes (synonymous) [4,6]. The ratio of nonsynonymous to synonymous changes due to SNPs varies between 0.3-1.0 [13] depending on the data set used. In our analysis of SNPs in exons, we see that there a ...
... of amino acid (nonsynonymous) are lower or about the same as substitutions which result in silent changes (synonymous) [4,6]. The ratio of nonsynonymous to synonymous changes due to SNPs varies between 0.3-1.0 [13] depending on the data set used. In our analysis of SNPs in exons, we see that there a ...
Identifikasi Molekular Bakteri Pathogen yang Menginfeksi Hama
... (Ishii et al., 2014b). Cecropins are used by insect as the main defence against gram-negative bacteria. Three different proteases exist in S. marcescens can destroy cecropins (Fly et al., 1983). It has been proposed that outer membrane vesicles (OMVs) produced by bacteria might act as long-range tox ...
... (Ishii et al., 2014b). Cecropins are used by insect as the main defence against gram-negative bacteria. Three different proteases exist in S. marcescens can destroy cecropins (Fly et al., 1983). It has been proposed that outer membrane vesicles (OMVs) produced by bacteria might act as long-range tox ...
Identity of zinc finger nucleases with specificity novel HSV-2 vaccine/therapy precursors
... Using the FASTA format of the nucleotide sequences of the whole genome of HSV-2 strain HG52 (Dolan et al. [27]) (provided in additional file 1) and CoDA-ZiFiT [34-36], a proprietary computational software of the ZFN-consortium that was pre-set at its default-user options, identification of over 28,0 ...
... Using the FASTA format of the nucleotide sequences of the whole genome of HSV-2 strain HG52 (Dolan et al. [27]) (provided in additional file 1) and CoDA-ZiFiT [34-36], a proprietary computational software of the ZFN-consortium that was pre-set at its default-user options, identification of over 28,0 ...
MAGMA manual (version 1.06)
... MAGMA detects a header in the file it will look for SNP IDs and p-values in the SNP and P column respectively. If no header is found it will use the first column for SNP IDs and the second column for p-values. If you want to use different columns instead, you can add the use modifier (with two value ...
... MAGMA detects a header in the file it will look for SNP IDs and p-values in the SNP and P column respectively. If no header is found it will use the first column for SNP IDs and the second column for p-values. If you want to use different columns instead, you can add the use modifier (with two value ...
Lactococcus lactis LM0230 contains a single
... step of many amino acids, may have important physiological functions in Lactococcus lactis during growth in milk. In this study, the aspartate ATase gene (aspC) from L. lactis LM0230 was cloned by complementation into Escherichia coli DL39. One chromosomal fragment putatively encoding aspC was parti ...
... step of many amino acids, may have important physiological functions in Lactococcus lactis during growth in milk. In this study, the aspartate ATase gene (aspC) from L. lactis LM0230 was cloned by complementation into Escherichia coli DL39. One chromosomal fragment putatively encoding aspC was parti ...
Quantitative genetics of gene expression during fruit fly - EMBL-EBI
... Taking advantage of these new possibilities, we performed a high-resolution eQTL study on 80 inbred fruit fly lines from the Drosophila Genetic Reference Panel, which represent naturally occurring genetic variation in a wild population of Drosophila melanogaster. Using a 3′ Tag RNA-sequencing protoc ...
... Taking advantage of these new possibilities, we performed a high-resolution eQTL study on 80 inbred fruit fly lines from the Drosophila Genetic Reference Panel, which represent naturally occurring genetic variation in a wild population of Drosophila melanogaster. Using a 3′ Tag RNA-sequencing protoc ...
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... repression. However, it was shown that the mechanism of PcGmediated repression can happen as well in the opposite order, with PRC1 introducing its modification prior to PRC2 activity (Yang et al., 2013; Del Prete et al., 2015). Polycomb-group complexes were identified also in the plant kingdom. Core ...
... repression. However, it was shown that the mechanism of PcGmediated repression can happen as well in the opposite order, with PRC1 introducing its modification prior to PRC2 activity (Yang et al., 2013; Del Prete et al., 2015). Polycomb-group complexes were identified also in the plant kingdom. Core ...