Relationships between a new type IV secretion system and the icm
... were retained. We suggested that these results could be explained either by a partial requirement for some dot/icm components, or alternatively by the presence of another conjugal system whose components might substitute for the missing dot/icm gene product (Segal and Shuman, 1998b; Segal et al., 19 ...
... were retained. We suggested that these results could be explained either by a partial requirement for some dot/icm components, or alternatively by the presence of another conjugal system whose components might substitute for the missing dot/icm gene product (Segal and Shuman, 1998b; Segal et al., 19 ...
An accessible database for mouse and human whole transcriptome
... To use the database via the UCSC genome browser, follow these steps: (1) In the browser, select organism: ‘human’ or ‘mouse’. (2) Browse to your gene of interest. (3) To view the track ‘Whole transcriptome qPCR primers’ scroll down the page to the panel of suggested tracks named ‘Expression’. Turn t ...
... To use the database via the UCSC genome browser, follow these steps: (1) In the browser, select organism: ‘human’ or ‘mouse’. (2) Browse to your gene of interest. (3) To view the track ‘Whole transcriptome qPCR primers’ scroll down the page to the panel of suggested tracks named ‘Expression’. Turn t ...
Transvection Is Common Throughout the Drosophila
... gen) of the vector used to introduce the CRM-GAL4. A schematic of an integrated CRM-GAL4 is shown in Figure 1A. While screening through CRM-GAL4s that had been integrated into attP2, we identified two transgenes, R24B02GAL4 and R58G08-GAL4, that drove expression in neuroblast hemilineages 12A and 3A ...
... gen) of the vector used to introduce the CRM-GAL4. A schematic of an integrated CRM-GAL4 is shown in Figure 1A. While screening through CRM-GAL4s that had been integrated into attP2, we identified two transgenes, R24B02GAL4 and R58G08-GAL4, that drove expression in neuroblast hemilineages 12A and 3A ...
"Frontmatter". In: Plant Genomics and Proteomics
... comparable processes of pattern formation and the underlying genes appear to be similar, the actual mechanisms of getting to the end points of development are different. Once again, this reinforces the need to look specifically at the plant processes in order to understand how plants function. One o ...
... comparable processes of pattern formation and the underlying genes appear to be similar, the actual mechanisms of getting to the end points of development are different. Once again, this reinforces the need to look specifically at the plant processes in order to understand how plants function. One o ...
Gene Signal Estimates from Exon Arrays v1.0
... of the probe sets are more exploratory in nature, being outside of regions that are annotated by RefSeq as being transcribed. The GeneChip® Human Exon 1.0 ST Array is inclusive by design. In addition to containing probe sets that interrogate exons of RefSeq (Pruitt and Maglott 2001) genes, mRNAs and ...
... of the probe sets are more exploratory in nature, being outside of regions that are annotated by RefSeq as being transcribed. The GeneChip® Human Exon 1.0 ST Array is inclusive by design. In addition to containing probe sets that interrogate exons of RefSeq (Pruitt and Maglott 2001) genes, mRNAs and ...
Progress in the Understanding of the Genetic Etiology of Vertebral
... be erroneous. Instead, a cis mechanism of interaction with Notch was demonstrated, one that results in inhibition of Notch signaling.30,31 Further DLL3 is predominantly expressed in the Golgi apparatus in contrast to other DSL ligands which are expressed on the cell surface.30 Phenotypic Analysis of ...
... be erroneous. Instead, a cis mechanism of interaction with Notch was demonstrated, one that results in inhibition of Notch signaling.30,31 Further DLL3 is predominantly expressed in the Golgi apparatus in contrast to other DSL ligands which are expressed on the cell surface.30 Phenotypic Analysis of ...
3. Inheritance and hereditary
... discrete trait, then the variation can be treated as a qualitative trait, which would simplify its analysis. For example, consider height as a phenotype you wish to study (Figure 3 [SGF-1025]). If the heights of an organism cluster into discrete classes (in the simplest case, tall and short, with no ...
... discrete trait, then the variation can be treated as a qualitative trait, which would simplify its analysis. For example, consider height as a phenotype you wish to study (Figure 3 [SGF-1025]). If the heights of an organism cluster into discrete classes (in the simplest case, tall and short, with no ...
ETHYLENE
... From Wilkinson, J.Q., Lanahan, M.B., Yen, H.-C., Giovannoni, J.J., and Klee, H.J. (1995). An ethylene-inducible component of signal transduction encoded by Neverripe. Science 270: 1807-1809, reprinted with permisison from AAAS; Lanahan, M.B., Yen, H.C., Giovannoni, J.J., and Klee, H.J. (1994). The N ...
... From Wilkinson, J.Q., Lanahan, M.B., Yen, H.-C., Giovannoni, J.J., and Klee, H.J. (1995). An ethylene-inducible component of signal transduction encoded by Neverripe. Science 270: 1807-1809, reprinted with permisison from AAAS; Lanahan, M.B., Yen, H.C., Giovannoni, J.J., and Klee, H.J. (1994). The N ...
An analysis of taenidial fold formation via the study of Blimp-1
... friendship, my life in Barcelona got much more fulfilling. I would like to also thank to my mother, father and brother for their limitless love and continuous support. Last but not least I would like to thank to Ilker, my love, my husband and my best friend. As many other things in my life, without ...
... friendship, my life in Barcelona got much more fulfilling. I would like to also thank to my mother, father and brother for their limitless love and continuous support. Last but not least I would like to thank to Ilker, my love, my husband and my best friend. As many other things in my life, without ...
special - Microbiology
... coelicolor possesses several genes with pleiotropic effects on antibiotic production. At least some of these are likely to play regulatory roles. The product of one, AfsR, is a homologue of ActII-ORF4 and RedD, with its N-terminal region showing 33 YOamino acid sequence identity to each of the pathw ...
... coelicolor possesses several genes with pleiotropic effects on antibiotic production. At least some of these are likely to play regulatory roles. The product of one, AfsR, is a homologue of ActII-ORF4 and RedD, with its N-terminal region showing 33 YOamino acid sequence identity to each of the pathw ...
CNS Tumors
... • Most often arise next to the ependyma-lined ventricular system, including the central canal of the spinal cord • Occurs in the first two decades of life, they typically occur near the fourth ventricle • In adults, the spinal cord is their most common location ...
... • Most often arise next to the ependyma-lined ventricular system, including the central canal of the spinal cord • Occurs in the first two decades of life, they typically occur near the fourth ventricle • In adults, the spinal cord is their most common location ...
Homologous Recombination Between Episomal Plasmids and Chromosomes in Yeast.
... strains was done on solid medium containing: 2% potassium acetate, 0.1% glucose, 0.25% Bactoyeast extract (Difco) and 1.5% Bacto-agar (Difco). Yeast transformation: Yeast strains were transformed using the procedure of HINNEN,HICKSand FINK (1978) with the following modification. T h e glusulase (End ...
... strains was done on solid medium containing: 2% potassium acetate, 0.1% glucose, 0.25% Bactoyeast extract (Difco) and 1.5% Bacto-agar (Difco). Yeast transformation: Yeast strains were transformed using the procedure of HINNEN,HICKSand FINK (1978) with the following modification. T h e glusulase (End ...
Replication of plasmids with the p15A origin in Shewanella
... Shewanella putrefaciens MR-1 (Myers and Nealson 1988a) and Escherichia coli JM109 (Yanisch-Perron et al. 1985) were grown aerobically on LB medium (Sambrook et al. 1989) supplemented, as needed, with antibiotics at the following concentrations : tetracycline (Tc), 8 mg ml−1 and chloramphenicol (Cm), ...
... Shewanella putrefaciens MR-1 (Myers and Nealson 1988a) and Escherichia coli JM109 (Yanisch-Perron et al. 1985) were grown aerobically on LB medium (Sambrook et al. 1989) supplemented, as needed, with antibiotics at the following concentrations : tetracycline (Tc), 8 mg ml−1 and chloramphenicol (Cm), ...
Early frameshift alleles of zebrafish tbx5a that fail to
... first coding exon (Fig. 1A)11. This sgRNA targets the coding sequence in the first coding exon downstream of the conserved translation initiation codon 12. We targeted the first exon to introduce frameshift and subsequent stop codons early in the open reading frame to avoid potential translation of ...
... first coding exon (Fig. 1A)11. This sgRNA targets the coding sequence in the first coding exon downstream of the conserved translation initiation codon 12. We targeted the first exon to introduce frameshift and subsequent stop codons early in the open reading frame to avoid potential translation of ...
Click
... This tutorial should have shown you the extensive resources at NCBI. The databases are interlinked, and it is possible to readily move between databases to mine all types of data from NCBI. Once you have found the gene that you are interested from either from an ENTREZ search or an OMIM search, you ...
... This tutorial should have shown you the extensive resources at NCBI. The databases are interlinked, and it is possible to readily move between databases to mine all types of data from NCBI. Once you have found the gene that you are interested from either from an ENTREZ search or an OMIM search, you ...
A novel role for the floral homeotic gene APETALA2
... 35S::BP gain-of-function fruits have greatly enlarged repla, and bp mutations enhance the reduced replum phenotype of rpl mutants (Alonso-Cantabrana et al., 2007). BP is negatively regulated in fruits (Alonso-Cantabrana et al., 2007) and in leaves (Byrne et al., 2000; Ori et al., 2000; Semiarti et a ...
... 35S::BP gain-of-function fruits have greatly enlarged repla, and bp mutations enhance the reduced replum phenotype of rpl mutants (Alonso-Cantabrana et al., 2007). BP is negatively regulated in fruits (Alonso-Cantabrana et al., 2007) and in leaves (Byrne et al., 2000; Ori et al., 2000; Semiarti et a ...
A mutation in α-tropomyosin affects muscle strength, maturation and
... lies in the major groove of filamentous α-actin and plays a regulatory role in muscle contraction which is dependent on Ca2+ ion concentration (23). The head and tail domains of the protein are believed to have a function in both dimerization and actin binding (22). The Met9Arg missense mutation is ...
... lies in the major groove of filamentous α-actin and plays a regulatory role in muscle contraction which is dependent on Ca2+ ion concentration (23). The head and tail domains of the protein are believed to have a function in both dimerization and actin binding (22). The Met9Arg missense mutation is ...
080201 Clinical and Molecular Genetic Features of Pulmonary
... Figure 1. Signaling Pathway of the Transforming Growth Factor b (TGF-b) Superfamily. In the extracellular space, ligands to the TGF-b superfamily of receptors bind either to an accessory protein, which presents the ligand to the type II receptor, or directly to the type II receptor on the cell membr ...
... Figure 1. Signaling Pathway of the Transforming Growth Factor b (TGF-b) Superfamily. In the extracellular space, ligands to the TGF-b superfamily of receptors bind either to an accessory protein, which presents the ligand to the type II receptor, or directly to the type II receptor on the cell membr ...
Genetic Basis for Osmosensitivity and Genetic Instability of the Wine
... a NADH-dependent glycerol-3-phosphate dehydrogenase, encoded by GPD1 that converts dihydroxyacetone phos‑ phate to glycerol-3-phosphate with the concomitant oxida‑ tion of NADH to NAD +. High levels of glycerol formation during osmotic stress lead to a redox imbalance in the yeast cell (NADH:NAD +). ...
... a NADH-dependent glycerol-3-phosphate dehydrogenase, encoded by GPD1 that converts dihydroxyacetone phos‑ phate to glycerol-3-phosphate with the concomitant oxida‑ tion of NADH to NAD +. High levels of glycerol formation during osmotic stress lead to a redox imbalance in the yeast cell (NADH:NAD +). ...
Repair of Site-Specific DNA Double-Strand Breaks in
... throughout the cell cycle, but predominantly occur during G1, when sister chromatids are not available (reviewed in Lieber, 2010). Moreover, both ends of a DSB may interact separately and follow different repair pathways, which result in various types (and combinations) of rearrangements. A nonrecip ...
... throughout the cell cycle, but predominantly occur during G1, when sister chromatids are not available (reviewed in Lieber, 2010). Moreover, both ends of a DSB may interact separately and follow different repair pathways, which result in various types (and combinations) of rearrangements. A nonrecip ...
Circadian Rhythm of Circumnutation in
... inflorescence stems of Arabidopsis to determine the circadian regulation of circumnutation. Under constant light conditions (LL), circumnutation speed in wild-type plants fluctuates, with the phase of the highest speed at subjective dawn; the period length is close to 24 h. toc1 appears to shorten t ...
... inflorescence stems of Arabidopsis to determine the circadian regulation of circumnutation. Under constant light conditions (LL), circumnutation speed in wild-type plants fluctuates, with the phase of the highest speed at subjective dawn; the period length is close to 24 h. toc1 appears to shorten t ...
Unit 3 Solutions - Manning`s Science
... about experimental setup (i.e., the use of two radioactive isotopes to differentially label DNA and capsid protein), experimental procedure (i.e., the use of agitation in a blender to dislodge viruses, and subsequent centrifugation), and results. A summary of the experiment is found in Figure 5.3 on ...
... about experimental setup (i.e., the use of two radioactive isotopes to differentially label DNA and capsid protein), experimental procedure (i.e., the use of agitation in a blender to dislodge viruses, and subsequent centrifugation), and results. A summary of the experiment is found in Figure 5.3 on ...
Journal Club 3
... • CcO is shown to be reversibly inhibited by low concentrations of NO • This proposal must be supported by further functional studies ...
... • CcO is shown to be reversibly inhibited by low concentrations of NO • This proposal must be supported by further functional studies ...
Centipede Hox genes - Development
... developmental genetics facilitates this, as it provides some basis for speculating about the developmental processes of other arthropods. The body plan of Drosophila is encoded in part by the patterned expression of a set of transcription factors called the Hox proteins, which divide the embryo into ...
... developmental genetics facilitates this, as it provides some basis for speculating about the developmental processes of other arthropods. The body plan of Drosophila is encoded in part by the patterned expression of a set of transcription factors called the Hox proteins, which divide the embryo into ...
Genome Biology - Institute for Applied Ecology
... whether genes in the valley and peaks on the Z chromosome were enriched in specific functions (Tables S2 and S3). Valley genes were found to be enriched in genes involved in regulation of cellular physiological process, regulation of DNA dependent transcription, reproductive system development and f ...
... whether genes in the valley and peaks on the Z chromosome were enriched in specific functions (Tables S2 and S3). Valley genes were found to be enriched in genes involved in regulation of cellular physiological process, regulation of DNA dependent transcription, reproductive system development and f ...