"Silencing of Gene Expression in Cultured Cells Using Small
... initiated a new wave of reverse genetics in mammalian systems. The siRNA-mediated silencing provides fast, target-specific down-regulation of gene expression. The extent to which one can successfully carry out the silencing for determination of gene function depends on the potency of siRNAs, transfe ...
... initiated a new wave of reverse genetics in mammalian systems. The siRNA-mediated silencing provides fast, target-specific down-regulation of gene expression. The extent to which one can successfully carry out the silencing for determination of gene function depends on the potency of siRNAs, transfe ...
Cytokinin and auxin have a crucial role in the control of this balance.
... As it reaches its site of action, auxin is perceived by receptors of the TRANSPORT INHIBITOR RESPONSE 1 (TIR1) family [38,39]. TIR1 encodes for an F-box subunit of the ubiquitin ligase complex SCFTIR1 (SKP1, CDC53/ CULLIN, F-box); when auxin binds to the TIR1 receptor, it stabilizes the interaction ...
... As it reaches its site of action, auxin is perceived by receptors of the TRANSPORT INHIBITOR RESPONSE 1 (TIR1) family [38,39]. TIR1 encodes for an F-box subunit of the ubiquitin ligase complex SCFTIR1 (SKP1, CDC53/ CULLIN, F-box); when auxin binds to the TIR1 receptor, it stabilizes the interaction ...
Fluorescent - Nanoimaging - Friedrich-Schiller
... Different groups of antibodies exist: Polyclonal antibodies They are a mixture of antibodies secreted against a specific antigen, each recognizing a different epitope i.e. bind to different areas of the protein. The protein (e.g. tubulin) for which a special antibody should be generated is injec ...
... Different groups of antibodies exist: Polyclonal antibodies They are a mixture of antibodies secreted against a specific antigen, each recognizing a different epitope i.e. bind to different areas of the protein. The protein (e.g. tubulin) for which a special antibody should be generated is injec ...
AnsteadSeniorHonorsThesis
... mainly using Mcm2 and Mcm5, suggest that proliferative malignant cells, precancerous cells, and potential cancer reoccurrence are marked by increased Mcm levels, especially in cervical and lung cancers, as well as meningioma (Williams, G. 1998; Tan, D. 2001; Hunt, D. 2002). Mcm10 could also play a ...
... mainly using Mcm2 and Mcm5, suggest that proliferative malignant cells, precancerous cells, and potential cancer reoccurrence are marked by increased Mcm levels, especially in cervical and lung cancers, as well as meningioma (Williams, G. 1998; Tan, D. 2001; Hunt, D. 2002). Mcm10 could also play a ...
Ectopic segmentation gene expression and
... terms of a combinatorial role for ftz, suggesting that it ...
... terms of a combinatorial role for ftz, suggesting that it ...
Negative regulation of defense responses in Arabidopsis by two
... analysis indicates that there are orthologs of NPR3 and NPR4 in rice (data not shown). To identify the functions of NPR3 and NPR4, deletion mutants of NPR3 and NPR4 were obtained by screening a fast neutron-mutagenized Arabidopsis population (Li et al., 2001b). These were named npr3-1 and npr4-3, re ...
... analysis indicates that there are orthologs of NPR3 and NPR4 in rice (data not shown). To identify the functions of NPR3 and NPR4, deletion mutants of NPR3 and NPR4 were obtained by screening a fast neutron-mutagenized Arabidopsis population (Li et al., 2001b). These were named npr3-1 and npr4-3, re ...
French 2003 - University of Washington
... often paddleless appendages, while overexpression either expands the operculum at the expense of appendage material or creates multiple, often antler-shaped dorsal structures. The subsequent events underlying dorsal appendage morphogenesis are only beginning to be understood. Analyses of cultured wi ...
... often paddleless appendages, while overexpression either expands the operculum at the expense of appendage material or creates multiple, often antler-shaped dorsal structures. The subsequent events underlying dorsal appendage morphogenesis are only beginning to be understood. Analyses of cultured wi ...
SGD sample annotations
... While the authors mention the use of a direct assay, it is only in passing in the Discussion section and no experiment is shown. Thus, we have used the evidence code NAS, to indicate that this annotation is based on an author statement that cannot be traced to another paper. Note that the main reaso ...
... While the authors mention the use of a direct assay, it is only in passing in the Discussion section and no experiment is shown. Thus, we have used the evidence code NAS, to indicate that this annotation is based on an author statement that cannot be traced to another paper. Note that the main reaso ...
open access - Max-Planck-Institut für Pflanzenzüchtungsforschung
... These factors are predicted to increase the relative rate of nonsynonymous to synonymous (dN/dS) substitution, potentially resulting in the fixation of deleterious alleles (11). Previous studies comparing the distribution of polymorphisms between rice and dogs and their closest wild relatives have su ...
... These factors are predicted to increase the relative rate of nonsynonymous to synonymous (dN/dS) substitution, potentially resulting in the fixation of deleterious alleles (11). Previous studies comparing the distribution of polymorphisms between rice and dogs and their closest wild relatives have su ...
Is it Good to Share? The Parallel between Information
... beneficial genes and facilitates the emergence of complex genomes. Early organisms needed HGT to get going. When heredity is good (small u) HGT is unfavourable because it creates junk DNA. Large genomes can be maintained successfully without HGT. This model does not account for disruption of existin ...
... beneficial genes and facilitates the emergence of complex genomes. Early organisms needed HGT to get going. When heredity is good (small u) HGT is unfavourable because it creates junk DNA. Large genomes can be maintained successfully without HGT. This model does not account for disruption of existin ...
Exam 2 Review Guide November 8, 2014, 12:30 pm to 2:30 pm
... Understand the key processes in the central dogma Know what does transcription and translation Know what an mRNA is Understand how to transcribe and translate a sequence of DNA as in Homework #3 Understand how the lac operon controls the proteins necessary for lactose use in a bacteria. Know the lac ...
... Understand the key processes in the central dogma Know what does transcription and translation Know what an mRNA is Understand how to transcribe and translate a sequence of DNA as in Homework #3 Understand how the lac operon controls the proteins necessary for lactose use in a bacteria. Know the lac ...
Localization of Three Genes in the Hook
... 300- 1000 discrete areas within the nucleus where DNA replication takes place, termed "replication foci" [10-12]. These domains may be related to the association of both transcription [13-17] and replication [18-201 with the nuclear matrix, a proteinaceous structural support of the nucleus [21]. Law ...
... 300- 1000 discrete areas within the nucleus where DNA replication takes place, termed "replication foci" [10-12]. These domains may be related to the association of both transcription [13-17] and replication [18-201 with the nuclear matrix, a proteinaceous structural support of the nucleus [21]. Law ...
Analysis of mutant strains
... If the diploid is able to grow in the absence of Met, complementation has occurred, and the met mutations in the two haploid strains must be in different genes. If the diploid is not able to survive on the selective plate, the two haploid strains carry mutations in the same gene (although they are ...
... If the diploid is able to grow in the absence of Met, complementation has occurred, and the met mutations in the two haploid strains must be in different genes. If the diploid is not able to survive on the selective plate, the two haploid strains carry mutations in the same gene (although they are ...
Mcbio 316: Exam 2 ANSWER KEY (10) 1. Proteins encoded by the
... Note that the three phage (A1, A2, and A8) are each infecting the same pair of bacterial strains. a. Suggest an explanation for each of the six results. ANSWER: • Phage A1 cannot lyse DB21 or the P22 lysogen of DB21, indicating that it cannot adsorb to or cannot complete replication and morphogenesi ...
... Note that the three phage (A1, A2, and A8) are each infecting the same pair of bacterial strains. a. Suggest an explanation for each of the six results. ANSWER: • Phage A1 cannot lyse DB21 or the P22 lysogen of DB21, indicating that it cannot adsorb to or cannot complete replication and morphogenesi ...
studies on human x-chromosomal disorder
... incurable, till today) have been found to have their origins on the X chromosome. Although there are numerous X-linked disorders, few very important diseases were considered to be analyzed using a bioinformatics approach. The discovery may throw some light on various findings which could be very use ...
... incurable, till today) have been found to have their origins on the X chromosome. Although there are numerous X-linked disorders, few very important diseases were considered to be analyzed using a bioinformatics approach. The discovery may throw some light on various findings which could be very use ...
Advanced Gene Mapping in Eukaryotes
... tetrad directly. That is, because haploid organisms have only one copy of each gene, the phenotype is the direct result of the allele that is present. In other words, dominance and recessiveness issues do not come into play as they do in diploid organisms. We will outline this technique shortly. Bef ...
... tetrad directly. That is, because haploid organisms have only one copy of each gene, the phenotype is the direct result of the allele that is present. In other words, dominance and recessiveness issues do not come into play as they do in diploid organisms. We will outline this technique shortly. Bef ...
... sensitivity to UV-C, continuous light, H2O2 and ionising radiation and exhibits early senescence and reduced seed yield. Expressed in all tissues examined Identification of mutant: Jiang et al. (1997), Hefner et al. (2003). Gene isolation: Hefner et al. At3g05210 (UVR7/AtERCC1) (2003). Mutants are s ...
Investigating Pedigrees Name: Example Pedigree: Example case
... page. Fill in each person’s genotype next to their symbol on the pedigree. If there genotype has more than one possibility, put them both down. Then, answer the questions below. Pedigree for Li and Mai’s families: ...
... page. Fill in each person’s genotype next to their symbol on the pedigree. If there genotype has more than one possibility, put them both down. Then, answer the questions below. Pedigree for Li and Mai’s families: ...
Worm research hits the fast lane
... used to perform drug and compound screens. He is also looking to extrapolate this technology to other model organisms, such as zebrafish and others that grow in liquid culture. “But we cannot extend it to Drosophila because it cannot be cultured in liquid,” says Yanik. This technology is drawing a l ...
... used to perform drug and compound screens. He is also looking to extrapolate this technology to other model organisms, such as zebrafish and others that grow in liquid culture. “But we cannot extend it to Drosophila because it cannot be cultured in liquid,” says Yanik. This technology is drawing a l ...
... sensitivity to UV-C, continuous light, H2O2 and ionising radiation and exhibits early senescence and reduced seed yield. Expressed in all tissues examined Identification of mutant: Jiang et al. (1997), Hefner et al. (2003). Gene isolation: Hefner et al. At3g05210 (UVR7/AtERCC1) (2003). Mutants are s ...
Mef2 gene expression marks the cardiac and skeletal muscle
... MEF2 DNA-binding activity is also present in neonatal cardiac myocytes, indicating that MEF2 expression can be controlled independently of the skeletal muscle bHLH proteins (Zhu et al., 1991; Yu et al., 1992; Navankasattusas et al., 1992; Ianello et al., 1991). The majority of cardiac gene control r ...
... MEF2 DNA-binding activity is also present in neonatal cardiac myocytes, indicating that MEF2 expression can be controlled independently of the skeletal muscle bHLH proteins (Zhu et al., 1991; Yu et al., 1992; Navankasattusas et al., 1992; Ianello et al., 1991). The majority of cardiac gene control r ...