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Multidimensional Analysis
Multidimensional Analysis

... • Feature selection: a selected group of genes may be optimal (t-test) • Independent validation: you must test the classifier on samples that were not used for feature selection or for building the classifier (training set - test set or leave-oneout crossvalidation) ...
Genetic Code & Mutations
Genetic Code & Mutations

... “Language of RNA” RNA contains 4 different bases: A,U,C,G  The genetic code is responsible for building all the proteins in the body using 20 different amino acids.  Codon: three consecutive nucleotides that specify a single amino acid. ...
Robust Gene Dys-Regulation in Alzheimer`s Brains
Robust Gene Dys-Regulation in Alzheimer`s Brains

... pathogenic mechanism, for which the brain transcriptome of AD offers some clues at the gene expression level. So far, thousands of genes have been reported to be dys-regulated in the brains of patients suffering from AD. However, the consistency or discrepancy among these studies has not been thorou ...
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... Plant microRNAs regulate gene expression in a sequence-specific manner by binding to target mRNAs, leading to their degradation. Unlike animal microRNAs, plant microRNAs have a high degree of complementarity to their targets, and the scarcity of microRNA lossof-function phenotypes in plants implies ...
A Lite Introduction toComparative Genomics
A Lite Introduction toComparative Genomics

... • SNP: Single Nucleotide Polymorphism - change in one base between two instances of the same gene • Used as genetic flags to identify traits, esp. for genetic diseases • CG goal: Identify as many SNPs as possible • Challenges – Data: need sequenced genomes from many humans along with information abo ...
Scientists have observed that when double
Scientists have observed that when double

... different transcription factors and enhancers, but does not understand that this would not occur during mRNA processing because promoters regulate gene expression before transcription, not after transcription. ...
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16 ge_vs_sb_NOTES

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2421_Ch9.ppt

... to clone eukaryotic genes, the processed mRNA is converted to cDNA by an enzyme called reverse transcriptase The resulting cDNA can then be inserted in a bacterial cell ...
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What is a Gene?

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... 8. _____: The bacterial RNA polymerase consists of a core enzyme (α2ββ’) and a sigma factor (σ). If we treated the enzyme with an antibiotic (rifamycin), the activity of a subunit β was inhibited by preventing initiation of transcription, prior to the formation of the first phospodiester bond. So th ...
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GMO and Biotechnology

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... Problems with expressing Eukaryotic genes in Bacteria • Eventhough bacteria are capable of transcribing and translating eukaryotic genes, they do not have the capacity to splice exons and get rid of introns. So in order for them to be able to make a protein, an in intronless gene has to be inserted ...
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Ribosome and Introduction to DNA Forensics

... the gain or loss 1. Ionic bonds are formed by ________________of electrons by an atom. Covalent bonds form by ________________ of electrons. the sharing 2. Cells contain four major families of small organic molecules, what are they? 1.____________ 2. nucleotides __________________3. ________________ ...
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genetics, dna replication, protein synthesis, biotechnology
genetics, dna replication, protein synthesis, biotechnology

... c. Hydrogen bonds form between bases d. Chromosomes condense 25. Which of the following is required for DNA replication to occur? a. DNA helicase b. DNA ligase ...
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... a genome-wide look into how organisms function, but also provide a whole new assortment of problems. Biological researchers have ever increasing sets of data with inadequate data integration, analysis, and discovery tools. Alone, integration of these large data sets is difficult because 1) each data ...
Gene Interaction that produces novel Phenotype
Gene Interaction that produces novel Phenotype

... • Genomic imprinting: differential expression of genetic material depending on whether it is inherited from the male or female parent. • Epigenetics: Phenomena due to alterations to DNA that do not include changes in the base sequence; often affect the way in which the DNA sequences are expressed. ...
Intro Biology Review for Final
Intro Biology Review for Final

... Note: Please remember that the final will be comprehensive. The final will be fill in the blank and multiple choice questions. Most questions will come straight from the powerpoints, so I would review those first and as you are doing this, please pay attention to the following list of terms and conc ...
What are mutations and how do they affect the production
What are mutations and how do they affect the production

... When the base sequence of DNA is __________________, the ______________________ sequence is also changed. Since amino acids chain together to create a specific ____________________, mutations could affect the operation of an ____________________, preventing it from properly catalyzing a reaction or ...
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File - Mrs. LeCompte

... Eukaryotic Cells Modify mRNA After Transcription Pre-mRNA = primary transcript that will be processed to functional mRNA 1) 5’ Cap = modified guanine nucleotide (guanosine triphosphate = GTP) that is added to the 5’ end of mRNA shortly after transcription begins - Protects the growing mRNA from degr ...
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Fathers and Mothers of Genetics

... (1822 – January 6, 1884) a german monk; referred to as the "father of genetics" for his study of the inheritance of traits in pea plants. Mendel showed that the inheritance of traits follows particular laws, which were later named after him. The significance of Mendel's work was not recognized until ...
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... Queen Victoria was a carrier of hemophilia. She had nine children and passed hemophilia on to several of them. All of her children married into the royal families of various countries of Europe. In this way, all of the Royal Families of Europe inherited the gene for hemophilia. ...
recombinant DNA - Cloudfront.net
recombinant DNA - Cloudfront.net

... restriction enzymes (restriction endonucleases): • Very specific – recognize and then cut DNA molecules at specific base sequences called a restriction site (recognition sequence) – These are often a symmetrical series of four to eight bases on both strands running in opposite directions. • If the r ...
Ch. 13 Genetic Engineering
Ch. 13 Genetic Engineering

... Those cells then continue to produce the protein coded for by the gene ...
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Therapeutic gene modulation

Therapeutic gene modulation refers to the practice of altering the expression of a gene at one of various stages, with a view to alleviate some form of ailment. It differs from gene therapy in that gene modulation seeks to alter the expression of an endogenous gene (perhaps through the introduction of a gene encoding a novel modulatory protein) whereas gene therapy concerns the introduction of a gene whose product aids the recipient directly.Modulation of gene expression can be mediated at the level of transcription by DNA-binding agents (which may be artificial transcription factors), small molecules, or synthetic oligonucleotides. It may also be mediated post-transcriptionally through RNA interference.
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