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S4 File
S4 File

... B. Similarity of variant frequency (VF) in blood and tumor DNA was tested for common variants as the % difference between VFtumor – VFblood with cut-offs arbitrarily set at 5% and 10%. Among 801 common variants in blood and tumor, VF was not preserved in the majority of the cases, while no correlati ...
Infectious Bursal Disease Virus (IBDV) genesig
Infectious Bursal Disease Virus (IBDV) genesig

... IBDV is a double stranded RNA virus that has a bi-segmented genome and belongs to the genus Avibirnavirus of family Birnaviridae. There are two distinct serotypes of the virus, but only serotype 1 viruses cause disease in poultry. At least six antigenic subtypes of IBDV serotype 1 have been identifi ...
Molecular analysis of Japanese patients with steroid 21
Molecular analysis of Japanese patients with steroid 21

... shown in Table 3. Among the 68 chromosomes, 56 (82%) were characterized by screening for the seven most common point mutations and deletions. Both chromosomes were affected by one of these mutations in 25 (73%) patients, 6 (18%) patients had only one copy of one of these mutations, and 3 (9%) patien ...
Chapter 5. Genetic Interactions and Pathways
Chapter 5. Genetic Interactions and Pathways

... we will describe methods for genes that act as switches in regulatory pathways and genes that interact in a metabolic pathway, as the logic used to order genes in these types of pathways differ. Included in this chapter are examples of how the analysis differs for recessive and dominant genes, and h ...
ABCA3 Gene Mutations in Newborns with Fatal
ABCA3 Gene Mutations in Newborns with Fatal

... 16 of the 21 infants (76 percent) (Fig. 2 and Table 2). These included homozygous nonsense mutations in codons 106 and 1142, a homozygous frameshift mutation, and heterozygous insertion mutations and splice-site mutations. Seven missense mutations were identified in conserved amino acids (Fig. 2), i ...
letters - Lewis-Sigler Institute | for Integrative Genomics
letters - Lewis-Sigler Institute | for Integrative Genomics

... significant regulation of MAT genes by the pheromone response pathway. The discrepancy could indicate that the S469I variant of Gpa1p affects regulation directly at MAT through a different pathway from that of exogenous pheromone. Alternatively, GPA1 might act indirectly through other regulators of ...
Characterization of the Arabidopsis thaliana Mutant pcb2 which
Characterization of the Arabidopsis thaliana Mutant pcb2 which

... in higher plants (Lange and Ghassemian 2003). Two glutamates form a porphobilinogen after the conversion to 5-aminolevulinic acids, and then four porphobilinogens make up a tetrapyrol ring that leads to protoporphyrin IX after some modifications. Insertion of a Mg ion into protoporphyrin IX by Mg-ch ...
No correlation between phenotype and genotype in boys with a
No correlation between phenotype and genotype in boys with a

... expressed protein acting as a global transcription repressor.4 The protein contains two domains, the 85 amino acid methylCpG binding domain (MBD) and the 102 amino acid transcriptional repression domain (TRD).4 5 Furthermore, a domain in the C terminal part of the protein, facilitating DNA binding a ...
Article The Landscape of Realized Homologous
Article The Landscape of Realized Homologous

... genomes from ten bacterial species and found all species have “hot” regions with elevated rates relative to the genome average. We examined the size, gene content, and chromosomal features associated with these regions and the correlations between closely related species. The recombination landscape ...
POSITION-EFFECT VARIEGATION AT SEVERAL
POSITION-EFFECT VARIEGATION AT SEVERAL

... in the presence of this additional! heterochromatin, appeared as completely recessive. Additional complications arose because the roughest3 phenotype is itself the result of a position effect brought about by a long inversion of the X chromosome which causes the roughest allele to be placed next to ...
Evolutionary consequences of polyploidy in prokaryotes and the
Evolutionary consequences of polyploidy in prokaryotes and the

... existed in the basal eukaryotes. The mating system of the halophilic archaeon Haloferax volcanii probably represents an intermediate stage between typical prokaryotic and eukaryotic sex. H. volcanii is highly polyploid, as well as many other Archaea. Here, we use computer simulation to explore genet ...
Open Access - Cambridge Neuroscience
Open Access - Cambridge Neuroscience

... using pooled DNA in two groups of U.K. samples, based on end of secondary/high school national academic exam achievement: high (n = 419) versus low (n = 183) mathematical ability while controlling for their verbal ability. Significant differences in allele frequencies between these groups were searc ...
Operon review
Operon review

... INACTIVE (Circle one) INACTIVE (Circle one) ...
Exploring Human Visual Pigments Copyright 2008, Gale Rhodes
Exploring Human Visual Pigments Copyright 2008, Gale Rhodes

... your search, in the colors that signify their BLAST scores, are located for you on the diagram. Notice that there are about 100 proteins (discovered so far, that is) that have 40% or more positives in alignment with red opsin. The opsins are members of the very large family of G protein-coupled rece ...
Lack of expression of XIST from a small ring X chromosome
Lack of expression of XIST from a small ring X chromosome

... To determine the effect of this sequence alteration we examined the X inactivation status of the chromosome carrying the variant. The primers that recognise the variant basepair flank two methylation-sensitive enzyme sites, which are known to be differentially methylated on the active and inactive X ...
Mendel`s Pea Plants
Mendel`s Pea Plants

... To study genetics, Mendel chose to work with pea plants for three reasons: 1) they have easily identifiable traits, 2) they grow quickly, and 3) they can self-pollinate or be crosspollinated. Self-pollination means that only one flower is involved; the flower’s pollen lands on its own reproductive o ...
Document
Document

... is: heterozygous. 8) When solving inheritance problems, use capital letters to represent dominant genes. 9) When solving inheritance problems, use lowercase letters to represent recessive genes. 10) A genotype is the combination of genes an individual ...
Notch activity induces Nodal expression and mediates the
Notch activity induces Nodal expression and mediates the

... 3H). Taken together, our results show that there are two conserved RBPjk recognition sites in the Nodal promoter, and that both sites are required for perinodal expression of Nodal in the mouse embryo. One of the earliest determinants of LR establishment in the chick embryo is the left-sided express ...
Isolation and identification of molecular partners of the proteins
Isolation and identification of molecular partners of the proteins

... uncontrolled growth and metastasis of cancerous cells (Bilder et al., 2000). An example is the development of colon cancer displayed in Figure 1. The gene products encoded by tumor suppressor genes fall into different categories including cell surface receptors such as Patched, cell adhesion molecul ...
Supplementary Table Legends
Supplementary Table Legends

... with the widest range of expression used for unsupervised clustering of TCGA and CRPC samples. The average RPKM difference between CRPC and TCGA primary prostate cancers is ...
The Ubiquitin-Proteasome Pathway and Plant Development
The Ubiquitin-Proteasome Pathway and Plant Development

... the need for an accessory protein like CIP8, as in the case of HY5 (Seo et al., 2003). In addition to its activity in the dark, COP1 may also target HY5 and LAF1 in the light through its interaction with members of the SPA family of proteins (Suppressor of phyA-105). SPA1 is a lightdependent repress ...
Biology 40S Genetics Booklet (StudentsCopy2)
Biology 40S Genetics Booklet (StudentsCopy2)

A versatile toolbox for PCR-based tagging of yeast genes: new
A versatile toolbox for PCR-based tagging of yeast genes: new

... protein complex purification (Gavin et al., 2002; Ho et al., 2002), as well as for single gene-function analysis. The strategy requires: (a) a pair of primers that contain within their 5 region sequences of homology to the genomic target location; and (b) PCR-cassettes (also termed ‘modules’) that ...
Molecular Evolution in Nonrecombining Regions of the Drosophila
Molecular Evolution in Nonrecombining Regions of the Drosophila

... ª The Author(s) 2012. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/ 3.0), which permi ...
mic.sgmjournals.org
mic.sgmjournals.org

... was observed and we focused our attention on lactate synthesis. Lactate synthesis was due to the ldh-encoded L-lactate dehydrogenase (Ldh). Features of Ldh activity and ldh transcription were analysed. The ldh gene was shown to be regulated at the transcriptional level by SugR, a pleiotropic transcr ...
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Therapeutic gene modulation

Therapeutic gene modulation refers to the practice of altering the expression of a gene at one of various stages, with a view to alleviate some form of ailment. It differs from gene therapy in that gene modulation seeks to alter the expression of an endogenous gene (perhaps through the introduction of a gene encoding a novel modulatory protein) whereas gene therapy concerns the introduction of a gene whose product aids the recipient directly.Modulation of gene expression can be mediated at the level of transcription by DNA-binding agents (which may be artificial transcription factors), small molecules, or synthetic oligonucleotides. It may also be mediated post-transcriptionally through RNA interference.
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