PSI - Bioinformatics Training Network (BTN)
... UCSC and UniProt, aims to provide a standard set of gene predictions for the human and mouse genomes • Considerable communication effort between curators from different groups is on-going ...
... UCSC and UniProt, aims to provide a standard set of gene predictions for the human and mouse genomes • Considerable communication effort between curators from different groups is on-going ...
A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus
... described the role of RPS3aE gene overexpression in salt tolerance. To characterize the role of AgRPS3aE in salt tolerance, recombinant species containing AgRPS3aE were constructed. The salt-tolerant properties of AgRPS3aE were demonstrated in M. oryzae and two model plant species, Arabidopsis and t ...
... described the role of RPS3aE gene overexpression in salt tolerance. To characterize the role of AgRPS3aE in salt tolerance, recombinant species containing AgRPS3aE were constructed. The salt-tolerant properties of AgRPS3aE were demonstrated in M. oryzae and two model plant species, Arabidopsis and t ...
Supplementary Methods
... microarrays shown in the top three panels of the Supplementary Figure are ABI Affymetrix (0.72), Agilent (0.84), and Illumina (0.68). The lower three panels in the Supplementary Figure shows a direct comparison of the fold-changes from the MAQC microarray results with the ExpressSeq measurements for ...
... microarrays shown in the top three panels of the Supplementary Figure are ABI Affymetrix (0.72), Agilent (0.84), and Illumina (0.68). The lower three panels in the Supplementary Figure shows a direct comparison of the fold-changes from the MAQC microarray results with the ExpressSeq measurements for ...
A defense-offense multi-layered regulatory switch in a pathogenic
... starts at the OFF state, when the level of the sRNA regulator is 0 and the TF is expressed and active (A), leading to a high level of protein 1 and a low level of protein 2 (B). Upon change in condition (ON step, at t = 10 h) the sRNA is activated, leading to a decrease in the level of the TF (A) as ...
... starts at the OFF state, when the level of the sRNA regulator is 0 and the TF is expressed and active (A), leading to a high level of protein 1 and a low level of protein 2 (B). Upon change in condition (ON step, at t = 10 h) the sRNA is activated, leading to a decrease in the level of the TF (A) as ...
Leukaemia Section t(7;14)(q21;q32) ERVWE1/IgH Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Cµ locus of IGH and the 5' upstream the 5' LTR (long terminal repeat) of ERVWE1 (94 bases upstream). However, the CDK6 gene lies 127 kb downstream ERVWE1, and it cannot be excluded that the target of the Immunoglobulin enhancer is CDK6 instead of ERVWE1 (ERVWE1 is from 91 935 631 to 91 945 186, and ...
... Cµ locus of IGH and the 5' upstream the 5' LTR (long terminal repeat) of ERVWE1 (94 bases upstream). However, the CDK6 gene lies 127 kb downstream ERVWE1, and it cannot be excluded that the target of the Immunoglobulin enhancer is CDK6 instead of ERVWE1 (ERVWE1 is from 91 935 631 to 91 945 186, and ...
Supplementary Text Comparisons of X and autosomal expression
... expression level comparisons between the X and autosomes and then show that X:AA expression ratios increase with increasing expression level cutoffs. However, we note that such an analysis is problematic due to circular reasoning. Specifically, if we assume that the X has indeed not been (completely ...
... expression level comparisons between the X and autosomes and then show that X:AA expression ratios increase with increasing expression level cutoffs. However, we note that such an analysis is problematic due to circular reasoning. Specifically, if we assume that the X has indeed not been (completely ...
RNA-Seq Tutorial - Gene Codes Corporation
... You begin by importing a variable file that classifies each of the samples into one of two categories. The variable file is just a text file with one condition for each sample in the same order as the data file. • Highlight “gene_exp” and go to F ...
... You begin by importing a variable file that classifies each of the samples into one of two categories. The variable file is just a text file with one condition for each sample in the same order as the data file. • Highlight “gene_exp” and go to F ...
plasmid
... Plasmid vector pBR322 First plasmid vectors to be developed was pBR322 (Bolivar et al., 1977), constructed by ligating restriction fragments from three naturally occurring E. coli plasmids: R1, R6.5 and pMB1. The pBR322 plasmid is small (just 4361 bp) having the origin of replication, markers for d ...
... Plasmid vector pBR322 First plasmid vectors to be developed was pBR322 (Bolivar et al., 1977), constructed by ligating restriction fragments from three naturally occurring E. coli plasmids: R1, R6.5 and pMB1. The pBR322 plasmid is small (just 4361 bp) having the origin of replication, markers for d ...
Isolation of DNA from A Single Helminth Using New Developed Kit
... and insoluble materials can easily disrupt the DNA binding carrier in the column and the DNA isolation can not be performed efficiently. Due to the insufficient lysis of the worms, the extraction of the DNA from worms directly after removing from 70% ethanol solution was not satisfactory and our res ...
... and insoluble materials can easily disrupt the DNA binding carrier in the column and the DNA isolation can not be performed efficiently. Due to the insufficient lysis of the worms, the extraction of the DNA from worms directly after removing from 70% ethanol solution was not satisfactory and our res ...
Chapter 3 Proteins: - California State University San Marcos
... ► Two homolgous DNA helices paired and held together by reciprocal exchg of two of the four strands ► Two pairs of strands: one pair of crossing strands and one pair or noncrossing ► Isomerization leads to open structure where both pairs occupy equivalent positions ► Holliday junction resolved by cu ...
... ► Two homolgous DNA helices paired and held together by reciprocal exchg of two of the four strands ► Two pairs of strands: one pair of crossing strands and one pair or noncrossing ► Isomerization leads to open structure where both pairs occupy equivalent positions ► Holliday junction resolved by cu ...
A spectrum of genes expressed during early stages of rice... flower development
... available for both these plant species. Genes expressed in a developmental-stage-specific and=or tissue-specific manner are of interest for various reasons. One compelling reason is the likelihood of identifying regulators of a pathway or identifying genes that in turn are regulated by such molecule ...
... available for both these plant species. Genes expressed in a developmental-stage-specific and=or tissue-specific manner are of interest for various reasons. One compelling reason is the likelihood of identifying regulators of a pathway or identifying genes that in turn are regulated by such molecule ...
Novel Function of the Eukaryotic Polypeptide
... AbstractThe mammalian GTP-binding protein GSPT, whose carboxy-terminal sequence is homologous to the eukaryotic elongation factor EF1α, binds to the polypeptide chain releasing factor eRF1 to function as eRF3 in translation termination. However, the amino-terminal domain of GSPT, which contains a p ...
... AbstractThe mammalian GTP-binding protein GSPT, whose carboxy-terminal sequence is homologous to the eukaryotic elongation factor EF1α, binds to the polypeptide chain releasing factor eRF1 to function as eRF3 in translation termination. However, the amino-terminal domain of GSPT, which contains a p ...
Molecular evidence for the origin of birds
... bird-mammal grouping do not appear saturated by substitutions. It is possible that a combination of these two factors (a higher rate of change in the lineages leading to birds and mammals and a G+C substitution bias) is responsible. In the case of myoglobin, there are no nucleotide sequences of the ...
... bird-mammal grouping do not appear saturated by substitutions. It is possible that a combination of these two factors (a higher rate of change in the lineages leading to birds and mammals and a G+C substitution bias) is responsible. In the case of myoglobin, there are no nucleotide sequences of the ...
Problem set 8 answers
... The mutant Kin-2 protein is active in the absence of functional Kin-1 protein. 8. The supply of nitrogen regulates yeast genes affecting nitrogen catabolism (Remember the prion lectures). The Ure2 protein of S. cerevisiae is a negative regulator of nitrogen catabolism that inhibits the Gln3 protein, ...
... The mutant Kin-2 protein is active in the absence of functional Kin-1 protein. 8. The supply of nitrogen regulates yeast genes affecting nitrogen catabolism (Remember the prion lectures). The Ure2 protein of S. cerevisiae is a negative regulator of nitrogen catabolism that inhibits the Gln3 protein, ...
Seed specific polycomb group gene and methods of use for same
... Drosophila. Polycomb group proteins also occur in animals and are highly conserved and ensure the stable inheritance of expression patterns through cell division as Well as control ...
... Drosophila. Polycomb group proteins also occur in animals and are highly conserved and ensure the stable inheritance of expression patterns through cell division as Well as control ...
Questions - National Biology Competition
... 15 moles of CO2 evolved per m2 per hour. 5 moles of CO2 evolved per m2 per hour. 0 moles of CO2 taken up and evolved per m2 per hour. 5 moles of CO2 taken up per m2 per hour. 10 moles of CO2 taken up per m2 per hour. ...
... 15 moles of CO2 evolved per m2 per hour. 5 moles of CO2 evolved per m2 per hour. 0 moles of CO2 taken up and evolved per m2 per hour. 5 moles of CO2 taken up per m2 per hour. 10 moles of CO2 taken up per m2 per hour. ...
Cardiovascular disease
... Hypertension is the most common disease in industrialized nations, with a prevalence above 20 percent in the general population. It imparts an increased risk of stroke, myocardial infarction, heart failure, and renal failure; many clinical trials have shown that reductions in blood pressure reduce t ...
... Hypertension is the most common disease in industrialized nations, with a prevalence above 20 percent in the general population. It imparts an increased risk of stroke, myocardial infarction, heart failure, and renal failure; many clinical trials have shown that reductions in blood pressure reduce t ...
mRNA over-expression/rescue
... Why inject mRNA? 1. Gain-of-function experiments (today): over & ectopic expression 2. Dominant negative (pseudo loss of function) expts 3. Mutant (today) or morpholino (tomorrow) rescue 4. Epistasis: what genes can and cannot rescue your mutant? Where does your mutant gene lie in a pathway? Why inj ...
... Why inject mRNA? 1. Gain-of-function experiments (today): over & ectopic expression 2. Dominant negative (pseudo loss of function) expts 3. Mutant (today) or morpholino (tomorrow) rescue 4. Epistasis: what genes can and cannot rescue your mutant? Where does your mutant gene lie in a pathway? Why inj ...
The Difference Makers
... that have come from “transposon fossils”? Possible student response: If the mobile elements of a transposon are not fully copied, either because of partial copying or a mutation, the transposon will no longer be able to move or jump on its own. Small and large RNAs and some proteins from recycled tr ...
... that have come from “transposon fossils”? Possible student response: If the mobile elements of a transposon are not fully copied, either because of partial copying or a mutation, the transposon will no longer be able to move or jump on its own. Small and large RNAs and some proteins from recycled tr ...
Lisa Byers UNIT 6: Genetic Transformations Unit Plan
... Unifying concepts: Make biotechnology relevant to the students and tie in how it relates to the science they have already learned. Discover how scientific processes can be used for many different purposes. Visually seeing how DNA goes to RNA, which then is turned into a protein that is expressed a t ...
... Unifying concepts: Make biotechnology relevant to the students and tie in how it relates to the science they have already learned. Discover how scientific processes can be used for many different purposes. Visually seeing how DNA goes to RNA, which then is turned into a protein that is expressed a t ...
Document
... encode the twenty standard amino acids, giving most amino acids more than one possible codon. There are also three 'stop' or 'nonsense' codons signifying the end of the coding region; these are the TAA, TGA and TAG codons. ...
... encode the twenty standard amino acids, giving most amino acids more than one possible codon. There are also three 'stop' or 'nonsense' codons signifying the end of the coding region; these are the TAA, TGA and TAG codons. ...
Gene Mutation Link With HIV Resistance
... this complex. These chaperone molecules can tag genes needed by the cell, pry open the appropriate stretch of chromatin, and initiate protein production. They can stitch chromatin shut to silence a gene. New research suggests that variations in this epigenetic machinery can be inherited, but not via ...
... this complex. These chaperone molecules can tag genes needed by the cell, pry open the appropriate stretch of chromatin, and initiate protein production. They can stitch chromatin shut to silence a gene. New research suggests that variations in this epigenetic machinery can be inherited, but not via ...
How Does Replication-Associated Mutational Pressure Influence
... ORFs were distributed evenly on the chromosome inorg and http://smorfland. microb.uni.wroc.pl). dependently of W or C strands or leading/lagging DNA The analysis of DNA walks on W and C strands strands. Figure 1b presents the same DNA walks for T. done for the third positions in coding sequences and ...
... ORFs were distributed evenly on the chromosome inorg and http://smorfland. microb.uni.wroc.pl). dependently of W or C strands or leading/lagging DNA The analysis of DNA walks on W and C strands strands. Figure 1b presents the same DNA walks for T. done for the third positions in coding sequences and ...