Molecular Plant-Microbe Interacttions
... inoculated by E. carotovora (5 × 108 CFU per ml) and plants were further grown for 24 h before analysis. Pre-treatment with P. polymyxa induced significant resistance: limited maceration was generally observed around the infection site (Fig. 1A), but the plants were still vital after 2 subsequent da ...
... inoculated by E. carotovora (5 × 108 CFU per ml) and plants were further grown for 24 h before analysis. Pre-treatment with P. polymyxa induced significant resistance: limited maceration was generally observed around the infection site (Fig. 1A), but the plants were still vital after 2 subsequent da ...
Citrinin enhances toxic and genotoxic effects of ochratoxin A, in vitro
... tumours in male mice. The aim of this study was to determine the cytotoxic and genotoxic combined effect of CIT and OTA, (i) in cells culture and (ii) in vivo on Dark Agouty rat fed for 3 weeks with ground wheat enriched with OTA and/or CIT. When the mycotoxins are simultaneously present, the cytoto ...
... tumours in male mice. The aim of this study was to determine the cytotoxic and genotoxic combined effect of CIT and OTA, (i) in cells culture and (ii) in vivo on Dark Agouty rat fed for 3 weeks with ground wheat enriched with OTA and/or CIT. When the mycotoxins are simultaneously present, the cytoto ...
Two-Exon Skipping Due to a Point Mutation in p67
... proline-rich sequence and the mutant protein may have lost its stability due to a loss of these sequences. The sequences, therefore, contributetoprotein stabilitythrough intra-or intermolecular association of p67-phox with itself or with other proteins such as p47-phox or p40-phox that contain simil ...
... proline-rich sequence and the mutant protein may have lost its stability due to a loss of these sequences. The sequences, therefore, contributetoprotein stabilitythrough intra-or intermolecular association of p67-phox with itself or with other proteins such as p47-phox or p40-phox that contain simil ...
Lab Meeting, Oct 16 2003
... Primer Testing • The bands which are approximately similar in size to the length of the original degenerate sequence are then cloned and sequenced to see if they share a homology to the QTL markers in tomato • The amplified PCR samples are inserted into a cloning Vector which is then inserted into ...
... Primer Testing • The bands which are approximately similar in size to the length of the original degenerate sequence are then cloned and sequenced to see if they share a homology to the QTL markers in tomato • The amplified PCR samples are inserted into a cloning Vector which is then inserted into ...
Structural variations in the human genome
... world, perhaps even in the entire universe. Its massive amount of base pairs consisting of a varying number of genes (per organism) contains hereditary information that is used in the development and functioning of an entire organism. In fact, it is hard to imagine life or living without DNA being i ...
... world, perhaps even in the entire universe. Its massive amount of base pairs consisting of a varying number of genes (per organism) contains hereditary information that is used in the development and functioning of an entire organism. In fact, it is hard to imagine life or living without DNA being i ...
Cloning, expression, sequence analysis and
... B. subtilis 168 autolysins in all of the above processes is speculative, as they have not been thoroughly investigated at the molecular level. B. subtilis 168 has two major vegetative cell autolysins, an amidase and a glucosaminidase of 50 and 90 kDa respectively, and of as yet This paper is dedicat ...
... B. subtilis 168 autolysins in all of the above processes is speculative, as they have not been thoroughly investigated at the molecular level. B. subtilis 168 has two major vegetative cell autolysins, an amidase and a glucosaminidase of 50 and 90 kDa respectively, and of as yet This paper is dedicat ...
UNC-115, a Conserved Protein with Predicted LIM and Actin
... in response to extracellular signals (Ridley and Hall, 1992; Ridley et al., 1992). Constitutively active forms of Cdc42, Rac, and Rho can alter actin cytoskeleton dynamics and cell morphology of cultured cells in distinct ways (Tapon and Hall, 1997). Some of these changes, such as filipodial and lam ...
... in response to extracellular signals (Ridley and Hall, 1992; Ridley et al., 1992). Constitutively active forms of Cdc42, Rac, and Rho can alter actin cytoskeleton dynamics and cell morphology of cultured cells in distinct ways (Tapon and Hall, 1997). Some of these changes, such as filipodial and lam ...
Neoplasia Lec4
... It is capable to damage DNA With extensive exposure to sunlight, the repair system is overwhelmed skin cancer They cause mutations in TP53 gene ...
... It is capable to damage DNA With extensive exposure to sunlight, the repair system is overwhelmed skin cancer They cause mutations in TP53 gene ...
Précis - Scoliosis and Spinal Disorders
... of genetic loci predisposing to IS: maximum evidence of allele-sharing in one family was detected for three loci on chromosome 6p, distal 10q, and 18q [23]. Another study found a linkage with IS at locus 17p11 in a three generation IS Italian family 9 and another linkage with IS was found at locus ...
... of genetic loci predisposing to IS: maximum evidence of allele-sharing in one family was detected for three loci on chromosome 6p, distal 10q, and 18q [23]. Another study found a linkage with IS at locus 17p11 in a three generation IS Italian family 9 and another linkage with IS was found at locus ...
Bruce Wallace Biotechnology Lab Program Student Guide 5th
... Beta lactamase, then, enables bacteria to reproduce in the presence of ampicillin. In addition, pARA carries a gene for the AraC protein, a protein that helps the bacterium make proteins encoded by genes inserted into this plasmid. A gene, even a foreign one, can be expressed (produced) if it is ins ...
... Beta lactamase, then, enables bacteria to reproduce in the presence of ampicillin. In addition, pARA carries a gene for the AraC protein, a protein that helps the bacterium make proteins encoded by genes inserted into this plasmid. A gene, even a foreign one, can be expressed (produced) if it is ins ...
Opening conference
... Plants are exposed to constantly changing environments and often have to survive in adverse conditions. Although numerous genes have been identified which control stress responses, the regulatory pathways by which plants tolerate salinity are still not well understood. To identify novel factors whic ...
... Plants are exposed to constantly changing environments and often have to survive in adverse conditions. Although numerous genes have been identified which control stress responses, the regulatory pathways by which plants tolerate salinity are still not well understood. To identify novel factors whic ...
Frequent Association of p53 Gene Mutation in
... cancers. Tumor suppressor genes, such as the retinoblastoma gene, were also implicated in a variety of cancers, and it is suggested that inactivation or loss of suppressor genes on a specific chromosome plays an important role in the develop ment of cancer and tumor progression. Recent studies have ...
... cancers. Tumor suppressor genes, such as the retinoblastoma gene, were also implicated in a variety of cancers, and it is suggested that inactivation or loss of suppressor genes on a specific chromosome plays an important role in the develop ment of cancer and tumor progression. Recent studies have ...
Technique ChIC and ChEC: Genomic Mapping of Chromatin Proteins
... Chromatin Endogenous Cleavage with Fixed Cells We demonstrate next that ectopic and endogenous genes can be tagged with micrococcal nuclease, which allows direct genomic mapping. This method is called ChEC, for chromatin endogenous cleavage. It is based on the reasoning that the Ca2⫹ level in the cy ...
... Chromatin Endogenous Cleavage with Fixed Cells We demonstrate next that ectopic and endogenous genes can be tagged with micrococcal nuclease, which allows direct genomic mapping. This method is called ChEC, for chromatin endogenous cleavage. It is based on the reasoning that the Ca2⫹ level in the cy ...
as a PDF
... that the reduction in expression was not caused by undesired sequence changes in the ocn promoter or lacZ coding sequence, but instead was a direct result of X linkage. Because the assays of b-galactosidase activity measure expression at the level of protein abundance, it is possible that they do no ...
... that the reduction in expression was not caused by undesired sequence changes in the ocn promoter or lacZ coding sequence, but instead was a direct result of X linkage. Because the assays of b-galactosidase activity measure expression at the level of protein abundance, it is possible that they do no ...
EIN4 and ERS2 Are Members of the Putative Ethylene Receptor
... the longest cDNA was approximately the same as the size of the corresponding RNA transcript on an RNA gel blot (data not shown), indicating that this cDNA is full length, or nearly so. Comparing the genomic DNA and cDNA sequences revealed that EIN4 contains one intron. The predicted protein has 766 ...
... the longest cDNA was approximately the same as the size of the corresponding RNA transcript on an RNA gel blot (data not shown), indicating that this cDNA is full length, or nearly so. Comparing the genomic DNA and cDNA sequences revealed that EIN4 contains one intron. The predicted protein has 766 ...
Mapping strategies for sequence reads (with focus on RNA-seq)
... ted by its splice site scoring algorithm; its authors mapping 71 million RNA-Seq reads to A.thaliana 0 CPU hours, which is ∼180 000 reads per CPU hour. ...
... ted by its splice site scoring algorithm; its authors mapping 71 million RNA-Seq reads to A.thaliana 0 CPU hours, which is ∼180 000 reads per CPU hour. ...
Exam 1
... There are many possible answers to this question, but the answer should describe a specific function for that particular class of mutation. Only one function was needed per class of mutation. Some examples include: Missense = can be used to study protein-protein interactions (e.g. by isolating allel ...
... There are many possible answers to this question, but the answer should describe a specific function for that particular class of mutation. Only one function was needed per class of mutation. Some examples include: Missense = can be used to study protein-protein interactions (e.g. by isolating allel ...
Differential chromatin packaging of genomic
... and one is the CpG methylation. Almost all imprinted genes have sequence elements that are methylated on only one of the two parental alleles. The differential methylation is a signal that leads to an inactive state of chromatin probably through binding to methyl-CpG-binding proteins, such as MeCP2, ...
... and one is the CpG methylation. Almost all imprinted genes have sequence elements that are methylated on only one of the two parental alleles. The differential methylation is a signal that leads to an inactive state of chromatin probably through binding to methyl-CpG-binding proteins, such as MeCP2, ...
Slide 1
... The specific amino acids in a polypeptide, and the order in which they are joined, determine the properties of different proteins. The sequence of amino acids influences the shape of the protein, which in turn determines its function. ...
... The specific amino acids in a polypeptide, and the order in which they are joined, determine the properties of different proteins. The sequence of amino acids influences the shape of the protein, which in turn determines its function. ...
Ribosomes and Proteins
... The specific amino acids in a polypeptide, and the order in which they are joined, determine the properties of different proteins. The sequence of amino acids influences the shape of the protein, which in turn determines its function. ...
... The specific amino acids in a polypeptide, and the order in which they are joined, determine the properties of different proteins. The sequence of amino acids influences the shape of the protein, which in turn determines its function. ...
Translation Section 1 From Genes to Proteins Chapter 10
... • Both prokaryotic and eukaryotic cells are able to regulate which genes are expressed and which are not, depending on the cell’s needs. • The piece of DNA that overlaps the promoter site and serves as the on-off switch is called an operator. ...
... • Both prokaryotic and eukaryotic cells are able to regulate which genes are expressed and which are not, depending on the cell’s needs. • The piece of DNA that overlaps the promoter site and serves as the on-off switch is called an operator. ...
forever young: a gene facilitating the study of the third larval instar of
... larvae. Some eventually pupate. Homozygous fey2 animals die at an earlier stage. The fey phenotype allows studies on behavior and tumorigenesis of third instar larvae. When placed on food containing 20-hydroxyecdysone (1 µg/ml), most fey homozygotes pupate though morphogenesis is arrested early. Mix ...
... larvae. Some eventually pupate. Homozygous fey2 animals die at an earlier stage. The fey phenotype allows studies on behavior and tumorigenesis of third instar larvae. When placed on food containing 20-hydroxyecdysone (1 µg/ml), most fey homozygotes pupate though morphogenesis is arrested early. Mix ...
... The development of mycobacterial genetic tools makes possible today the inactivation of selected genes, allowing the study of the molecular basis of pathogenicity in Mycobacterium tuberculosis. In addition genomic studies permit the comparison of virulent and avirulent M. tuberculosis strains. We we ...
externial ophtlhalmiioplegila has been reported as inherited in two
... family is recessive and sex-linked. As far as we have been able to discover in the literature there is no earlier reference to inheritance of this ty-pe for this particular disease. The clinical and neurological examination carried out by one of us (J. C. (). de Z.) on all fourteen living members of ...
... family is recessive and sex-linked. As far as we have been able to discover in the literature there is no earlier reference to inheritance of this ty-pe for this particular disease. The clinical and neurological examination carried out by one of us (J. C. (). de Z.) on all fourteen living members of ...
ANNEX III GUIDANCE ON COMMONLY USED VIRAL VECTORS
... Residual "latent" virus can also be associated with lymphoid tissue. Modification of the virus (e.g. to the fibre protein gene) might alter its tissue tropism. As a result, additional tissues might become susceptible to a modified virus. b) Altered infectivity and pathogenicity: Either the vectors t ...
... Residual "latent" virus can also be associated with lymphoid tissue. Modification of the virus (e.g. to the fibre protein gene) might alter its tissue tropism. As a result, additional tissues might become susceptible to a modified virus. b) Altered infectivity and pathogenicity: Either the vectors t ...