Cloning and expression of the phosphotriesterase
... The cloning of a gene encoding the novel phosphotriesterase from Pseudomonas monteilii C11, which enabled it to use the organophosphate (OP) coroxon as its sole phosphorus source, is described. The gene, called hocA (hydrolysis of coroxon) consists of 501 bp and encodes a protein of 19 kDa. This pro ...
... The cloning of a gene encoding the novel phosphotriesterase from Pseudomonas monteilii C11, which enabled it to use the organophosphate (OP) coroxon as its sole phosphorus source, is described. The gene, called hocA (hydrolysis of coroxon) consists of 501 bp and encodes a protein of 19 kDa. This pro ...
Analysis continued Each TopHat run will result in four files: a list of
... Running CuffCompare results in a number of different datasets. The transcript accuracy dataset calculates the accuracy of each of the transcripts as compared to the reference at various levels (nucleotide, exon, intron, transcript, gene), e.g., how often an exon that was predicted by the output from ...
... Running CuffCompare results in a number of different datasets. The transcript accuracy dataset calculates the accuracy of each of the transcripts as compared to the reference at various levels (nucleotide, exon, intron, transcript, gene), e.g., how often an exon that was predicted by the output from ...
DNA SEQUENCING (using a Li
... To label and separate DNA fragments varying by single nucleotides, in order to determine the sequence of nucleotides. ...
... To label and separate DNA fragments varying by single nucleotides, in order to determine the sequence of nucleotides. ...
Studies of the Growth Hormone-Prolactin Gene Family and their
... (90-100% sequence identity, corresponding to the last ~40 million years of human evolution), with the particular enrichment of genes involved in immunity and defense, membrane surface interactions, drug detoxification as well as growth and development (Bailey et al. 2002). Following the duplication ...
... (90-100% sequence identity, corresponding to the last ~40 million years of human evolution), with the particular enrichment of genes involved in immunity and defense, membrane surface interactions, drug detoxification as well as growth and development (Bailey et al. 2002). Following the duplication ...
Polyamines and other charged amines bind to RNA by hydrogen
... proposed models for spermine and spermidine, NH 2 (CH 2 ) NH(CH2) NH2,binding to DNA in which phosphate groups interact with each positively charged amino group, the tetramethylene portion of the polyamine bridges the narrow (minor) groove of the helix between the two strands and the trimethylene po ...
... proposed models for spermine and spermidine, NH 2 (CH 2 ) NH(CH2) NH2,binding to DNA in which phosphate groups interact with each positively charged amino group, the tetramethylene portion of the polyamine bridges the narrow (minor) groove of the helix between the two strands and the trimethylene po ...
Disrupting antibiotic resistance propagation by inhibiting
... shares significant sequence identity with relaxases of many R plasmids (e.g., 98% with R100 TraI); thus, the F plasmid serves as a model system for examining conjugative plasmids and the inhibition of conjugative transfer. In this study we first sought to understand the role that the relaxase enzyme ...
... shares significant sequence identity with relaxases of many R plasmids (e.g., 98% with R100 TraI); thus, the F plasmid serves as a model system for examining conjugative plasmids and the inhibition of conjugative transfer. In this study we first sought to understand the role that the relaxase enzyme ...
Francis Harry Compton Crick – Nobel Lecture
... respectively). This discovery has opened the way to a rapid although somewhat confused attack on the genetic code. It would not be appropriate to review this work in detail here. I have discussed critically the earlier work in the review mentioned previously1 but such is the pace of work in this fie ...
... respectively). This discovery has opened the way to a rapid although somewhat confused attack on the genetic code. It would not be appropriate to review this work in detail here. I have discussed critically the earlier work in the review mentioned previously1 but such is the pace of work in this fie ...
Analysis of the histone H3 gene family in Arabidopsis and
... read-through transcript of At5g65360 and a new intron is generated in this transcript, which was determined by DNA sequencing. ...
... read-through transcript of At5g65360 and a new intron is generated in this transcript, which was determined by DNA sequencing. ...
Pole Region-Dependent Repression of the Drosophila Gap Gene
... Scott and O’Farrell, 1986). Gap genes are required for the normal expression of pair-rule genes. The activity of the pair-rule genes establishes parasegmentai units (Martinez-Arias and Lawrence, 1985) and regulates both homeotic gene activities and the expression of segment-polarity genes (Ingham an ...
... Scott and O’Farrell, 1986). Gap genes are required for the normal expression of pair-rule genes. The activity of the pair-rule genes establishes parasegmentai units (Martinez-Arias and Lawrence, 1985) and regulates both homeotic gene activities and the expression of segment-polarity genes (Ingham an ...
Distinguishing coding from non-coding sequences in a prokaryote
... where T P denotes the number of correctly recognized coding sequences, F N the number of coding sequences recognized as non-coding sequences, F P the number of noncoding sequences recognized as coding sequences, and T N the number of correctly recognized non-coding sequences. Then we use the algorit ...
... where T P denotes the number of correctly recognized coding sequences, F N the number of coding sequences recognized as non-coding sequences, F P the number of noncoding sequences recognized as coding sequences, and T N the number of correctly recognized non-coding sequences. Then we use the algorit ...
silicon nanowire biosensor for studying nuclear hormone receptor
... Estrogen receptors (ERs) are members of a superfamily of nuclear receptors that function as ligand-modulated transcriptional regulators. The ERs mainly function as a DNA binding transcription factor that regulates gene expression. They play a key role in many normal physiological processes, but prom ...
... Estrogen receptors (ERs) are members of a superfamily of nuclear receptors that function as ligand-modulated transcriptional regulators. The ERs mainly function as a DNA binding transcription factor that regulates gene expression. They play a key role in many normal physiological processes, but prom ...
as a PDF
... not shown). Another region of GUS staining was detected in the root-shoot junction in which the adventitious roots are initiated. In roots, GUS staining was detected all over the pericycle and parenchyma of the vascular cylinder (Figures 2C and 2D). The pericycle cells retain a potential to divide, ...
... not shown). Another region of GUS staining was detected in the root-shoot junction in which the adventitious roots are initiated. In roots, GUS staining was detected all over the pericycle and parenchyma of the vascular cylinder (Figures 2C and 2D). The pericycle cells retain a potential to divide, ...
Two-Component System of Mycobacterium Tuberculosis
... Two Component Systems (TCSs), are widespread signal transduction devices in prokaryotes that enable these organisms to elicit an adaptive response to environmental stimuli, primarily through changes in gene expression [1]. They are typically composed of a membrane-located sensor with histidine kinas ...
... Two Component Systems (TCSs), are widespread signal transduction devices in prokaryotes that enable these organisms to elicit an adaptive response to environmental stimuli, primarily through changes in gene expression [1]. They are typically composed of a membrane-located sensor with histidine kinas ...
cdc2a Expression in Arabidopsis 1s Linked with
... not shown). Another region of GUS staining was detected in the root-shoot junction in which the adventitious roots are initiated. In roots, GUS staining was detected all over the pericycle and parenchyma of the vascular cylinder (Figures 2C and 2D). The pericycle cells retain a potential to divide, ...
... not shown). Another region of GUS staining was detected in the root-shoot junction in which the adventitious roots are initiated. In roots, GUS staining was detected all over the pericycle and parenchyma of the vascular cylinder (Figures 2C and 2D). The pericycle cells retain a potential to divide, ...
Single Gene
... Wild-type human eye color is brown - Blue and green eyes stemmed from mutations that persisted Melanin synthesis confers eye color, and this is controlled by a single gene. However, a second gene controls expression of the first gene, and a mutation in this gene abolishes that control and blue eyes ...
... Wild-type human eye color is brown - Blue and green eyes stemmed from mutations that persisted Melanin synthesis confers eye color, and this is controlled by a single gene. However, a second gene controls expression of the first gene, and a mutation in this gene abolishes that control and blue eyes ...
v2 PEBiosystems News 2 01/00
... AmpliTaq Gold, GeneAmp and TaqMan are registered trademarks of Roche Molecular System, Inc. ICAT is a trademark of the University of Washington, exclusively licensed to Applied Biosystems Group of Applera Corporation. Paracel is a registered trademark of Paracel Inc. The ABI PRISM 3100 and the 3100- ...
... AmpliTaq Gold, GeneAmp and TaqMan are registered trademarks of Roche Molecular System, Inc. ICAT is a trademark of the University of Washington, exclusively licensed to Applied Biosystems Group of Applera Corporation. Paracel is a registered trademark of Paracel Inc. The ABI PRISM 3100 and the 3100- ...
Mechanistic Models of Cancer in the Space of Pathways
... P53 TSG • P53 is a transcription factor that inhibits cell growth and stimulates cell death ...
... P53 TSG • P53 is a transcription factor that inhibits cell growth and stimulates cell death ...
Acute Phase Proteins - a Potent Biomarker for Mastitis
... activated at the cytoplasmic side of the IL-6 receptor by phosphorylation. Once activated it translocates to the nucleus. In mice, the three main regulatory elements of the Hp gene promoter are two recognition sites for the transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) flanking a ...
... activated at the cytoplasmic side of the IL-6 receptor by phosphorylation. Once activated it translocates to the nucleus. In mice, the three main regulatory elements of the Hp gene promoter are two recognition sites for the transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) flanking a ...
genotyping arabidopsis - STLCC.edu :: Users` Server
... called “Activator” (Ac) is necessary for the transposition of Ds. Ds is believed to be a mutant transposon, lacking the gene for transposase. Ac carries the transposase gene, and no matter where in the genome it is located, it supplies the enzyme for the transposition of Ds. It is possible to cross ...
... called “Activator” (Ac) is necessary for the transposition of Ds. Ds is believed to be a mutant transposon, lacking the gene for transposase. Ac carries the transposase gene, and no matter where in the genome it is located, it supplies the enzyme for the transposition of Ds. It is possible to cross ...
The human genome. Implications for medicine and society
... constituted another point of contention between Celera and public HGP. In 1998, when Celera announced the commencement of decoding the human genome, at the same time stated its intention to acquire the “rights” (patents) for several genes10. This announcement forced the public HGP to increase the pa ...
... constituted another point of contention between Celera and public HGP. In 1998, when Celera announced the commencement of decoding the human genome, at the same time stated its intention to acquire the “rights” (patents) for several genes10. This announcement forced the public HGP to increase the pa ...
How Should Species Phylogenies Be Inferred from
... and Carpenter, 1996) concatenates all available nucleotides (or amino acids) for a set of taxa into a single matrix for analysis. In doing so, simultaneous analysis collapses what should be two levels of analysis into one. This results in three problems: First, and probably most important, the simul ...
... and Carpenter, 1996) concatenates all available nucleotides (or amino acids) for a set of taxa into a single matrix for analysis. In doing so, simultaneous analysis collapses what should be two levels of analysis into one. This results in three problems: First, and probably most important, the simul ...
Regulation of cell fusion in C. elegans - Development
... decision. Perhaps both Hox proteins bind to regulatory sites in a cell fusion gene that encodes a direct effector of cell fusion. In this model, the binding of either Hox protein alone would inhibit expression of this gene while binding of both Hox proteins would activate expression. To investigate ...
... decision. Perhaps both Hox proteins bind to regulatory sites in a cell fusion gene that encodes a direct effector of cell fusion. In this model, the binding of either Hox protein alone would inhibit expression of this gene while binding of both Hox proteins would activate expression. To investigate ...
MOLECULAR BIOLOGY AND BIOTECHNOLOGY
... MBB 502/ FUNDAMENTALS OF MOLECULAR BIOLOGY 3+0 SEM - I BIOCHEM 504/ (To be taught jointly by Molecular Biology & Biotechnology, Biochemistry and BIF508 Bioinformatics) ObjectiveT o familiarize the students with the basic cellular processes at molecular level. Theory UNIT-I: Historical developments o ...
... MBB 502/ FUNDAMENTALS OF MOLECULAR BIOLOGY 3+0 SEM - I BIOCHEM 504/ (To be taught jointly by Molecular Biology & Biotechnology, Biochemistry and BIF508 Bioinformatics) ObjectiveT o familiarize the students with the basic cellular processes at molecular level. Theory UNIT-I: Historical developments o ...
Importance of integrons in the diffusion of resistance
... not contain the sul1 gene but in fact include genes whose function promotes Tn7 transposition [36, 72, 73, 88]. To date, only one class 3 integron has been reported, containing the blaIMP gene cassette that confers resistance to broadspectrum β-lactams including carbapenems, and part of the aacA4 ge ...
... not contain the sul1 gene but in fact include genes whose function promotes Tn7 transposition [36, 72, 73, 88]. To date, only one class 3 integron has been reported, containing the blaIMP gene cassette that confers resistance to broadspectrum β-lactams including carbapenems, and part of the aacA4 ge ...