modification Identifying regulatory mechanisms using individual
... fraction of the variance: explaining ⬎50% PGV for 828 genes, as compared with 238 in the analysis of Brem and Kruglyak (16) of the same data set. Our comparison to three simpler Geronemo models suggests that most of the improvement results from the incorporation of trans-E regulation, which captures ...
... fraction of the variance: explaining ⬎50% PGV for 828 genes, as compared with 238 in the analysis of Brem and Kruglyak (16) of the same data set. Our comparison to three simpler Geronemo models suggests that most of the improvement results from the incorporation of trans-E regulation, which captures ...
Genetics - Mount Mansfield Union High School
... • The expected frequency of a particular event when an experiment is repeated an infinite number of times is the probability of the event. • Probabilities in genetics are often predicted based on certain hypotheses and then the predictions are used to test the hypothesis using real data. ...
... • The expected frequency of a particular event when an experiment is repeated an infinite number of times is the probability of the event. • Probabilities in genetics are often predicted based on certain hypotheses and then the predictions are used to test the hypothesis using real data. ...
A new DNA computing model for the NAND gate based on induced
... • Then ligase is added to seal the nick between the the gate strand and the output strand. • Output strand z at the end of these gate strands whose output is “1”, while those whose output is “0” remain ...
... • Then ligase is added to seal the nick between the the gate strand and the output strand. • Output strand z at the end of these gate strands whose output is “1”, while those whose output is “0” remain ...
Functional and Evolutionary Analysis of Flowering Time Genes in
... plants lack sex chromosomes; polyploidy in animals almost always leads to sterility. Another fact is that plant pollen is constantly spread across species boundaries. However, in most cases hybridization is hindered by pollen incompatibility. Polyploidization is believed to fuel plant speciation by ...
... plants lack sex chromosomes; polyploidy in animals almost always leads to sterility. Another fact is that plant pollen is constantly spread across species boundaries. However, in most cases hybridization is hindered by pollen incompatibility. Polyploidization is believed to fuel plant speciation by ...
Cancer What is cancer? Properties of cancer cells Properties of
... p53 protein (short half life, nuclear localization), led to the notion that wild type p53 could play a positive role in cell proliferation. This idea was strengthed by the work of Mercer and collaborators (Mercer et al. 1984; Mercer et al. 1982). Microinjection of p53 antibody (200.47 and PAb122) in ...
... p53 protein (short half life, nuclear localization), led to the notion that wild type p53 could play a positive role in cell proliferation. This idea was strengthed by the work of Mercer and collaborators (Mercer et al. 1984; Mercer et al. 1982). Microinjection of p53 antibody (200.47 and PAb122) in ...
Cancer
... protein is coprecipitated with the large-T antigen (Chang et al. 1979; Kress et al. 1979; Lane and Crawford 1979; Linzer and Levine 1979; Melero et al. 1979). This association was shown to be the result of an in vivo association between the two proteins (Lane and Crawford 1979). It was then postulat ...
... protein is coprecipitated with the large-T antigen (Chang et al. 1979; Kress et al. 1979; Lane and Crawford 1979; Linzer and Levine 1979; Melero et al. 1979). This association was shown to be the result of an in vivo association between the two proteins (Lane and Crawford 1979). It was then postulat ...
Expression of the Ly-6E.1 - Development
... fragment containing the 14 kb Ly-6E.1 genomic sequence with the inserted lacZ gene was obtained from pL6LZ and designated BL (for BamH1-lacZ). The truncated Ly-6E.1-lacZ fragment was obtained by XbaI and NotI digestion of pL6LZ and designated XN (for XbaI/NotI). Both fragments were gel-purified for ...
... fragment containing the 14 kb Ly-6E.1 genomic sequence with the inserted lacZ gene was obtained from pL6LZ and designated BL (for BamH1-lacZ). The truncated Ly-6E.1-lacZ fragment was obtained by XbaI and NotI digestion of pL6LZ and designated XN (for XbaI/NotI). Both fragments were gel-purified for ...
Homeotic selector genes
... • many HOM-C genes have autoregulatory binding sites in their promoters – but positive feedback, in itself, is insufficient to maintain memory without other factors – another group of genes, the Polycomb group, are required to repress homeotic selector genes that should not be expressed in a particu ...
... • many HOM-C genes have autoregulatory binding sites in their promoters – but positive feedback, in itself, is insufficient to maintain memory without other factors – another group of genes, the Polycomb group, are required to repress homeotic selector genes that should not be expressed in a particu ...
Association of the T-cell regulatory gene CTLA4 with susceptibility to
... there are two very common CTLA4 haplotypes, one susceptible for Graves’ disease, the other, protective (Supplementary Table B4). This result along with the single-point-association analysis (Fig. 1 and Supplementary Table B1), a Graves’ disease family-based study (Supplementary Table B1) and the two ...
... there are two very common CTLA4 haplotypes, one susceptible for Graves’ disease, the other, protective (Supplementary Table B4). This result along with the single-point-association analysis (Fig. 1 and Supplementary Table B1), a Graves’ disease family-based study (Supplementary Table B1) and the two ...
Functional binding of secreted molecules to heparan sulfate
... Particularly, these secreted factors pattern tissues in a concentration-dependent manner. Thus, regulating the extracellular distribution of Dpp is critical to patterning. In developing discs, a reduction in Dpp level increases the defects associated with dally mutations in the eye, antenna and geni ...
... Particularly, these secreted factors pattern tissues in a concentration-dependent manner. Thus, regulating the extracellular distribution of Dpp is critical to patterning. In developing discs, a reduction in Dpp level increases the defects associated with dally mutations in the eye, antenna and geni ...
DNA-Based Technologies
... no environmental influence on simple traits like coat color, and usually a single gene is responsible for the phenotype. However, in the case of complex traits, each marker is only associated with one of the genes that contributes toward the phenotype. Both “marked” and “unmarked” genes, in conjunct ...
... no environmental influence on simple traits like coat color, and usually a single gene is responsible for the phenotype. However, in the case of complex traits, each marker is only associated with one of the genes that contributes toward the phenotype. Both “marked” and “unmarked” genes, in conjunct ...
A twist in sea urchin gastrulation and mesoderm specification
... Abstract The bHLH (basic helix-loop-helix) transcription factor, sea urchin myogenic factor-1 (SUM-1), plays an important role in myogenic determination during sea urchin embryogenesis. SUM-1-mediated transactivation is restricted to the mesenchyme lineages in transgenic sea urchin embryos, suggesti ...
... Abstract The bHLH (basic helix-loop-helix) transcription factor, sea urchin myogenic factor-1 (SUM-1), plays an important role in myogenic determination during sea urchin embryogenesis. SUM-1-mediated transactivation is restricted to the mesenchyme lineages in transgenic sea urchin embryos, suggesti ...
Comparison of Genomes using High-Performance - FACOM
... Consider two genomes of close evolutionary relationship. The term homology is used to indicate the fact that two genes share a common evolutionary past. Two genes are homologous if they are descendants of a same ancestral gene. Often the similarity between two sequences of DNA or amino acids is impo ...
... Consider two genomes of close evolutionary relationship. The term homology is used to indicate the fact that two genes share a common evolutionary past. Two genes are homologous if they are descendants of a same ancestral gene. Often the similarity between two sequences of DNA or amino acids is impo ...
Extensive post-transcriptional regulation of miRNAs within
... of miRNA synthesis plays an important role in various human diseases including cancer. The biogenesis of miRNAs lends itself to regulation at several stages, from transcription of the miRNA gene to post-transcriptional control of primary, precursor and mature miRNA transcripts. We have analysed miRN ...
... of miRNA synthesis plays an important role in various human diseases including cancer. The biogenesis of miRNAs lends itself to regulation at several stages, from transcription of the miRNA gene to post-transcriptional control of primary, precursor and mature miRNA transcripts. We have analysed miRN ...
SVD and PCA
... Deflation • Once we have found an eigenvector e1 with eigenvalue 1, can compute matrix A – 1 e1 e1 T • This makes eigenvalue of e1 equal to 0, but has no effect on other eigenvectors/values • In principle, could find all eigenvectors this way ...
... Deflation • Once we have found an eigenvector e1 with eigenvalue 1, can compute matrix A – 1 e1 e1 T • This makes eigenvalue of e1 equal to 0, but has no effect on other eigenvectors/values • In principle, could find all eigenvectors this way ...
Molecular Evolution of Two Linked Genes, Est-6 and Sod, in
... times higher for lbl than for lbe (Tables 1 and 2). The difference was mostly due to variation in lbl exon II, where synonymous K/ = 18.89 (D. melanogaster – D. simulans) and 26.35 (D. melanogaster – D. sechellia). These differences suggest that the pressure to conserve synonymous sites of the codi ...
... times higher for lbl than for lbe (Tables 1 and 2). The difference was mostly due to variation in lbl exon II, where synonymous K/ = 18.89 (D. melanogaster – D. simulans) and 26.35 (D. melanogaster – D. sechellia). These differences suggest that the pressure to conserve synonymous sites of the codi ...
View PDF - Molecular Systems Biology
... occasion, but I hope that you will find the reviews below useful. _______________________ Reviewer #1: The authors modify existing single cell RNA-seq protocols in order to identify polyadenylation sites at single molecule resolution. They also introduce a novel statistical framework to interpret th ...
... occasion, but I hope that you will find the reviews below useful. _______________________ Reviewer #1: The authors modify existing single cell RNA-seq protocols in order to identify polyadenylation sites at single molecule resolution. They also introduce a novel statistical framework to interpret th ...
A DNA Polymerase ε Mutant That Specifically Causes 1
... that possess 3⬘ → 5⬘ exonuclease proofreading activity. Many errors arising during replication are corrected by these exonuclease activities. We have investigated the contributions of regions of Polε other than the proofreading motifs to replication accuracy. An allele, pol2-C1089Y, was identified i ...
... that possess 3⬘ → 5⬘ exonuclease proofreading activity. Many errors arising during replication are corrected by these exonuclease activities. We have investigated the contributions of regions of Polε other than the proofreading motifs to replication accuracy. An allele, pol2-C1089Y, was identified i ...
Amplified fragment length polymorphism (AFLP)
... against this damaging insect. To date, 27 resistance genes (H1–H27) have been reported in wheat; among these, 11 are very effective in Morocco. In this study, we have utilized amplified fragment length polymorphism (AFLP) analysis in conjunction with near-isogenic lines (NILs) and bulked segregant a ...
... against this damaging insect. To date, 27 resistance genes (H1–H27) have been reported in wheat; among these, 11 are very effective in Morocco. In this study, we have utilized amplified fragment length polymorphism (AFLP) analysis in conjunction with near-isogenic lines (NILs) and bulked segregant a ...
Hd6, a rice quantitative trait locus involved in photoperiod
... series of genes and show continuous variation in progeny. Many efforts have been made to map such genes, known as quantitative trait loci (QTLs), because of their biological and agronomic importance. The development of a high-density linkage map based on DNA markers made it possible to map QTLs with ...
... series of genes and show continuous variation in progeny. Many efforts have been made to map such genes, known as quantitative trait loci (QTLs), because of their biological and agronomic importance. The development of a high-density linkage map based on DNA markers made it possible to map QTLs with ...
Phylogenetic Portrait of the Saccharomyces cerevisiae Functional
... 20% of C. elegans genes are orthologous and that these proteins carry out a set of core biological processes (intermediary metabolism, DNA/RNA metabolism, protein folding, trafficking, and degradation; Chervitz et al. 1998). The major limitation of this study was that yeast and worm were the only com ...
... 20% of C. elegans genes are orthologous and that these proteins carry out a set of core biological processes (intermediary metabolism, DNA/RNA metabolism, protein folding, trafficking, and degradation; Chervitz et al. 1998). The major limitation of this study was that yeast and worm were the only com ...
Genomic approaches for the understanding of aging
... chain (ETC). Mutations in genes involved in the activity of mitochondrial ETC, such as isp-1 and clk-1, significantly increase lifespan (24, 25). Genomic screening of C. elegans chromosome I using a RNAi library showed that RNAi knockdown of genes encoding mitochondrial ETC components and mitochondr ...
... chain (ETC). Mutations in genes involved in the activity of mitochondrial ETC, such as isp-1 and clk-1, significantly increase lifespan (24, 25). Genomic screening of C. elegans chromosome I using a RNAi library showed that RNAi knockdown of genes encoding mitochondrial ETC components and mitochondr ...
Complete Thyroxine-Binding Globulin (TBG) Deficiency Produced
... mRNA transcripts from both expressing (liver) and nonexpressing (fibroblast) cells were indistinguishable at the site of the mutation. Although illegitimate transcription should, theoretically, amplify minute amounts of any mRNA present in all tissues, the amount of mRNA in nonexpressing tissues dep ...
... mRNA transcripts from both expressing (liver) and nonexpressing (fibroblast) cells were indistinguishable at the site of the mutation. Although illegitimate transcription should, theoretically, amplify minute amounts of any mRNA present in all tissues, the amount of mRNA in nonexpressing tissues dep ...
