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... 26 exons ranging in size from 69 to 3,106 bp, and its 25 introns range in size from 207 to 32,400 bp. The complete gene comprises ~9 kb of exon and ~177 kb of intron. • The biggest human gene yet is for dystrophin. It has >30 exons and is spread over 2.4 million bp. ...
The Story of Molecular Biology and Its Creators
The Story of Molecular Biology and Its Creators

... “Once information has passed into protein it cannot get out again”… Crick’s choice of the word “dogma” was not a call for blind faith in what was really a central hypothesis. According to Horace Judson in his book The Eighth Day of Creation, it was because Crick had it in his mind that “a dogma was ...
The Story of Molecular Biology and Its Creators
The Story of Molecular Biology and Its Creators

... “Once information has passed into protein it cannot get out again”… Crick’s choice of the word “dogma” was not a call for blind faith in what was really a central hypothesis. According to Horace Judson in his book The Eighth Day of Creation, it was because Crick had it in his mind that “a dogma was ...
Notes and Study Questions
Notes and Study Questions

... I.B. Strategies for identifying binding sites for regulatory protein So how can we find and identify binding sites for regulatory protein? You’ll see one clue by examining the sequence bound by CRP (Fig. 1C). Notice that there is a 7-bp sequence on one strand that is almost identical to a 7-bp seque ...
Information Flow in Eukaryotes (PowerPoint) Madison 2008
Information Flow in Eukaryotes (PowerPoint) Madison 2008

... we accommodated both linear and non-linear approaches to solving the problem. Tidbit 1 also engaged kinesthetic and visual learners. We used grapes rather than wine (alcohol) as an example. We used an example from the plant/agricultural field because not all students are pre-med. We were sensitive t ...
1 CHAPTER 3- DNA FUNCTION – THE EXPRESSION OF GENETIC
1 CHAPTER 3- DNA FUNCTION – THE EXPRESSION OF GENETIC

... Transfer RNAs – tRNAs – transport amino acids to RNA during protein synthesis Ribosomal RNAs – rRNAs – component of the ribosome Small nuclear RNAs – snRNAs – involved in RNA processing in eukaryotes Small cytoplasmic RNAs – scRNAs – protein trafficking in eukaryotes 2. How is RNA made from DNA? TRA ...
Genetic Engineering
Genetic Engineering

... • Each member of the parental generation transmits half of its hereditary factors to each offspring • Different sets of offspring from the same parents receive different sets of hereditary factors – Ex= siblings are not identical, their differences come from the inheritance of different genes from t ...
Structure of DNA (Deoxyribonucleic acid)
Structure of DNA (Deoxyribonucleic acid)

... ribosome to direct protein synthesis – makes mRNA • Occurs in the nucleus; occurs at only 1 gene at a time • A in DNA pairs with U (Uracil) in RNA, not T; T in DNA pairs with A in RNA • Steps: • Segment of DNA unwinds and unzips • RNA polymerase joins the RNA nucleotides that are complementary to th ...
The polymerase chain reaction (PCR)
The polymerase chain reaction (PCR)

... making an error about once every 8 million base pairs. Before Taq polymerase, other polymerases were used, but many of them broke down at the necessary temperatures for the reaction to commence. The heating cycle is complicated, but includes temperatures briefly ranging almost all the way to the boi ...
cells
cells

... 44. What is the reason for so many different proteins existing, when there are only 20 amino acids? -Each protein is made from a different combination and number of amino acids. ...
Unit 6 - John Adams Academy
Unit 6 - John Adams Academy

... Began studying crosses involving only one trait at a time  Crossed a pure-breeding strain of red flowered pea plants with a pure-breeding strain of white flowered pea plants ...
They are the offspring of these two people They are the
They are the offspring of these two people They are the

... Every organism exhibits one or more of the traits of their grandparents. Your description could involve; via the people who married into the family, by the expression of a recessive trait, via mutation. The children share more traits with parents than the grandchildren share. The children share more ...
Chapter 18: Regulation of Gene Expression - Biology E
Chapter 18: Regulation of Gene Expression - Biology E

... Almost all the cells in an organism contain an identical genome. (Cells of the immune system are one exception.) However, the subset of genes expressed in the cells of each type is unique, allowing these cells to carry out their specific function. The difference between cell types are due to differ ...
Key for Practice Exam 4
Key for Practice Exam 4

... RNA on a membrane is probed with a DNA probe. They differ because in a Southern Blot it is DNA on a membrane that is probed, whereas, in a Northern Blot is it RNA on a membrane that is probed. Also, a Southern blot tells whether a certain DNA sequence is present or not, and a Northern blot tells if ...
Intest Aid IB - SpeechNutrients.eu
Intest Aid IB - SpeechNutrients.eu

... The DNA in a cell consists of a long pattern made up of four different nucleotide bases. ...
Biotechnology and Genetic Engineering
Biotechnology and Genetic Engineering

... crown gall disease by first transferring part of its DNA into an opening in the plant. The DNA then integrates itself into the plant's genome and causes the formation of the gall. ...
Chapter 4 Outline
Chapter 4 Outline

... a. The sides of the ladder are made up of _________________________. b. The rungs of the ladder are made up of ________________________. 3. Before a cell divides, its DNA duplicates itself by unwinding and separating its ...
Bacterial species
Bacterial species

... What modification neutralizes the charges on histones and loosens up the interactions between histones and DNA? 1. phosphorylation 2. demethylation 3. acetylation 4. polyadenylation ...
Bingo Questions
Bingo Questions

...  Cell(s) – the simplest living unit that can have a life of its own  Tissue(s) – a group of cells with the same function (ie. – muscle, nervous tissue, etc.)  Organ(s) – a group of tissues that serve a specific function  Organ System(s) – a group of organs working in concert for a specific funct ...
Fall Semester Exam Review PDF
Fall Semester Exam Review PDF

... 18. When a substance is being moved into a cell, against the concentration gradient, such as A in the image below is this active or passive transport? ...
Junk DNA - repetitive sequences
Junk DNA - repetitive sequences

... including PDHA1, although it is essential for function of all cells. This lacking function was apparently rescued by retrotransposition - there is closely related gene PDHA2 on chromosome 4 - and this gene is intronless - a typical feature of processed pseudogenes.. Highly expressed housekeeping gen ...
7 October 2015 The Royal Swedish Academy of Sciences has
7 October 2015 The Royal Swedish Academy of Sciences has

... information. Their work has provided fundamental knowledge of how a living cell functions and is, for instance, used for the development of new cancer treatments. Each day our DNA is damaged by UV radiation, free radicals and other carcinogenic substances, but even without such external attacks, a D ...
Chapter 13
Chapter 13

... • Consider the formula on page 406 for probability of finding a particular fragment in N clones • Suppose you seek a 99% probability of finding a given fragment in N clones of 10 kbp fragments • If your library is from the human genome, you would need 1,400,000 clones to reach 99% probability of fin ...
introduction1
introduction1

... Central dogma of information flow in genetics ...
13.4 Gene Regulation and Expression
13.4 Gene Regulation and Expression

... Eukaryotic Gene Regulation Transcription factors are DNA-binding proteins. They control the expression of genes in eukaryotes by binding DNA sequences in the regulatory regions. Gene promoters have multiple binding sites for transcription factors, each of which can influence transcription. ▶ Complex ...
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Primary transcript



A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.
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