Expression of the six chromate ion transporter
... fragments were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and ligated into the pGEM-T vector. Recombinant plasmids were transferred by electroporation to E. coli JM101, selecting transformants on LB agar plates with 100 mg ampicillin ml21. The cloning process was verified by ...
... fragments were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and ligated into the pGEM-T vector. Recombinant plasmids were transferred by electroporation to E. coli JM101, selecting transformants on LB agar plates with 100 mg ampicillin ml21. The cloning process was verified by ...
Shoot Branching and Leaf Dissection in Tomato Are
... N-terminal part containing the two MYB repeats is highly conserved (see Supplemental Figure 1 online), but sequence similarity in the C-terminal domains can be detected only between specific pairs of sequences (Bl to Bli2 and Bli6, RAX3 to MYB68, and MYB36 to Bli8) and is not significant between oth ...
... N-terminal part containing the two MYB repeats is highly conserved (see Supplemental Figure 1 online), but sequence similarity in the C-terminal domains can be detected only between specific pairs of sequences (Bl to Bli2 and Bli6, RAX3 to MYB68, and MYB36 to Bli8) and is not significant between oth ...
Control of GL2 expression in Arabidopsis leaves and trichomes
... The expression pattern of the GL2 gene • In order to look at where the GL2 gene was being translated, the researchers made a special T-DNA construct • It was composed of a 5’UTR 2,1kb fragment of the GL2 gene and the b-glucuronidase gene (GUS) – GL2::GUS, (essentially the GUS gene under the control ...
... The expression pattern of the GL2 gene • In order to look at where the GL2 gene was being translated, the researchers made a special T-DNA construct • It was composed of a 5’UTR 2,1kb fragment of the GL2 gene and the b-glucuronidase gene (GUS) – GL2::GUS, (essentially the GUS gene under the control ...
Multi-petal cyclamen flowers produced by
... ornamental plants would be highly valuable, because this would confer both an interesting appearance and complete sterility, which would prevent the dispersal of transgenic seeds and pollen. Moreover, this trait could be produced in a selected variety with other valuable traits such as perfume or a ...
... ornamental plants would be highly valuable, because this would confer both an interesting appearance and complete sterility, which would prevent the dispersal of transgenic seeds and pollen. Moreover, this trait could be produced in a selected variety with other valuable traits such as perfume or a ...
peas? - Westgate Mennonite Collegiate
... These slides are provided as a teaching resource. You are encouraged to modify them to meet your specific teaching and learning needs. Please adhere to the copyright conditions specified on the following slide. There is a reference slide at the end of the presentation that lists the sources for the ...
... These slides are provided as a teaching resource. You are encouraged to modify them to meet your specific teaching and learning needs. Please adhere to the copyright conditions specified on the following slide. There is a reference slide at the end of the presentation that lists the sources for the ...
Gregor Mendel
... • 1. Plant traits are handed down through “hereditary factors” in the sex cells. • 2. Because offspring obtain hereditary factors from both parents, each plant must contain two factors for every trait. • 3. The factors in a pair segregate (separate) during the formation of the sex cells, and each sp ...
... • 1. Plant traits are handed down through “hereditary factors” in the sex cells. • 2. Because offspring obtain hereditary factors from both parents, each plant must contain two factors for every trait. • 3. The factors in a pair segregate (separate) during the formation of the sex cells, and each sp ...
2n gametes in the potato: essential ingredients for breeding and
... Genetic consequences associated with 2n gamete production Each meiotic mutation leading to 2n gametes is genetically equivalent to either first-division restitution (FDR) or second-division restitution (SDR) mechanisms (Peloquin 1983). Thus, for example, ps is equivalent to FDR, whereas os is equiva ...
... Genetic consequences associated with 2n gamete production Each meiotic mutation leading to 2n gametes is genetically equivalent to either first-division restitution (FDR) or second-division restitution (SDR) mechanisms (Peloquin 1983). Thus, for example, ps is equivalent to FDR, whereas os is equiva ...
quantitative genetics - E-Learning/An
... seeds. The alleles that govern these traits affect the phenotype in a qualitative way. In analyzing crosses involving these types of traits, each offspring can be put into a particular phenotypic category. Such attributes are called discontinuous traits. In contrast, quantitative traits show a conti ...
... seeds. The alleles that govern these traits affect the phenotype in a qualitative way. In analyzing crosses involving these types of traits, each offspring can be put into a particular phenotypic category. Such attributes are called discontinuous traits. In contrast, quantitative traits show a conti ...
mendelian inheritance - E-Learning/An
... gametes are produced in plants and fungi. These processes are described in greater detail in Chapter 3. In some experiments, Mendel wanted to carry out selffertilization, which means that the pollen and egg are derived from the same plant. In peas, a modified petal known as the keel covers the repro ...
... gametes are produced in plants and fungi. These processes are described in greater detail in Chapter 3. In some experiments, Mendel wanted to carry out selffertilization, which means that the pollen and egg are derived from the same plant. In peas, a modified petal known as the keel covers the repro ...
reactions of sweet corn hybrids to prevalent diseases
... trials are probably more accurate than estimates based on one or two trials. Symptoms may occur on hybrids rated R, but the amount of the disease on these hybrids is less than the amount on hybrids rated MR, M, MS, or S. Similarly, the effects of diseases on yield should correspond to this scale of ...
... trials are probably more accurate than estimates based on one or two trials. Symptoms may occur on hybrids rated R, but the amount of the disease on these hybrids is less than the amount on hybrids rated MR, M, MS, or S. Similarly, the effects of diseases on yield should correspond to this scale of ...
Lesson Overview
... Dominant and Recessive Traits Mendel’s second conclusion is called the principle of dominance. This principle states that some alleles are dominant and others are recessive. An organism with at least one dominant allele for a particular form of a trait will exhibit that form of the trait. An organis ...
... Dominant and Recessive Traits Mendel’s second conclusion is called the principle of dominance. This principle states that some alleles are dominant and others are recessive. An organism with at least one dominant allele for a particular form of a trait will exhibit that form of the trait. An organis ...
Universal chloroplast integration and expression vectors
... The invention provides universal chloroplast integration and expression vectors which are competent to stably transform and integrate genes of interest into chloroplast genome of multiple species of plants. Transformed plants and their progeny are provided. Monocotyledonous and dicotyledonous plants ...
... The invention provides universal chloroplast integration and expression vectors which are competent to stably transform and integrate genes of interest into chloroplast genome of multiple species of plants. Transformed plants and their progeny are provided. Monocotyledonous and dicotyledonous plants ...
Slide 1
... During sexual reproduction, male and female reproductive cells join in a process known as fertilization to produce a new cell. In peas, this new cell develops into a tiny embryo encased within a seed. ...
... During sexual reproduction, male and female reproductive cells join in a process known as fertilization to produce a new cell. In peas, this new cell develops into a tiny embryo encased within a seed. ...
Applied and Environmental Microbiology
... region were cloned (32). A leucine-responsive regulatory protein (LRP)-like gene (lrpL), which is located immediately upstream of acdS, is required for the expression of ACC deaminase in 128C53K. Neither the acdS nor the lrpL gene knockout mutant of 128C53K produces ACC deaminase, and both of the mu ...
... region were cloned (32). A leucine-responsive regulatory protein (LRP)-like gene (lrpL), which is located immediately upstream of acdS, is required for the expression of ACC deaminase in 128C53K. Neither the acdS nor the lrpL gene knockout mutant of 128C53K produces ACC deaminase, and both of the mu ...
Traits and Alleles Laboratory
... weather traits controlled by dominant alleles were more common than traits controlled by recessive alleles. The significance behind conducting this lab was to gain a better understanding on why people look the way they do and if there were common trends in which traits were seen more commonly. At th ...
... weather traits controlled by dominant alleles were more common than traits controlled by recessive alleles. The significance behind conducting this lab was to gain a better understanding on why people look the way they do and if there were common trends in which traits were seen more commonly. At th ...
The axr4 auxin-resistant mutants of Arabidopsis thaliana define a
... One striking aspect of the axr4 mutant phenotype is defective root gravitropism. To assess this defect quantitatively, vertically grown seedlings were reoriented by 90° and the time course of root tip curvature was measured. Plants of both alleles were consistently delayed in their response to gravi ...
... One striking aspect of the axr4 mutant phenotype is defective root gravitropism. To assess this defect quantitatively, vertically grown seedlings were reoriented by 90° and the time course of root tip curvature was measured. Plants of both alleles were consistently delayed in their response to gravi ...
Plant Molecular Biology
... extreme end ofnodM. It is known that transposon insertions at the very end of genes can leave the protein products derived from these genes active. Strain RBL5580 harbouring pMP180 : : TnphoA derivatives in which the transposon was ...
... extreme end ofnodM. It is known that transposon insertions at the very end of genes can leave the protein products derived from these genes active. Strain RBL5580 harbouring pMP180 : : TnphoA derivatives in which the transposon was ...
REVIEW ARTICLE Gene cassettes
... However, despite this heterogeneity, all 59-base elements share several features. They are bounded by an inverse core site (RYYYAAC) at the end closest to the 3’ end of the gene coding region and a core site (GTTRRRY) at the other end, and are related to a consensus sequence that covers approximatel ...
... However, despite this heterogeneity, all 59-base elements share several features. They are bounded by an inverse core site (RYYYAAC) at the end closest to the 3’ end of the gene coding region and a core site (GTTRRRY) at the other end, and are related to a consensus sequence that covers approximatel ...
Implementation of molecular markers for quantitative traits in
... Markers have been most widely used in practical plant breeding to assist backcrossing of major genes into elite cultivars that were previously developed through conventional plant breeding and to select alleles with major effects on high-value traits when marker information is reliable across multip ...
... Markers have been most widely used in practical plant breeding to assist backcrossing of major genes into elite cultivars that were previously developed through conventional plant breeding and to select alleles with major effects on high-value traits when marker information is reliable across multip ...
Classical Genetics - Morinville Community High School
... Many of you are already familiar with many of the genetic traits of humans such as baldness, eye color, color blindness and blood types. However, other characters such as size and position of eyes, number and shape of fingers, total body size and body proportion may also be genetically determined (a ...
... Many of you are already familiar with many of the genetic traits of humans such as baldness, eye color, color blindness and blood types. However, other characters such as size and position of eyes, number and shape of fingers, total body size and body proportion may also be genetically determined (a ...
Compound leaves: equal to the sum of their parts?
... reduced, which can be interpreted as a reduction in complexity (Molinero-Rosales et al., 1999). Known expression patterns of FLO/LFY orthologs in vegetative apices have been summarized recently (Busch and Gleissberg, 2003). In species with compound leaves, such as pea, tomato, grapevine and poppy, F ...
... reduced, which can be interpreted as a reduction in complexity (Molinero-Rosales et al., 1999). Known expression patterns of FLO/LFY orthologs in vegetative apices have been summarized recently (Busch and Gleissberg, 2003). In species with compound leaves, such as pea, tomato, grapevine and poppy, F ...
Occurrence of Multiple Antibiotic Resistant Bacteria in Aquatic
... attributed to both the natural environment and human overuse of these antibiotics for infections. The mere presence of antibiotic resistance in bacteria means danger to the future of antibiotics GXHWREDFWHULD¶VDELOLW\WRWUDQVIHUJHQHV(most importantly, antibiotic resistant genes) now known as h ...
... attributed to both the natural environment and human overuse of these antibiotics for infections. The mere presence of antibiotic resistance in bacteria means danger to the future of antibiotics GXHWREDFWHULD¶VDELOLW\WRWUDQVIHUJHQHV(most importantly, antibiotic resistant genes) now known as h ...
Journal of Bacteriology
... root exudate (15). As naringenin, apigenin, and some other flavonoids can replace exudate for the induction of the nodABC promoter (21), we investigated whether naringenin or apigenin alone is sufficient to replace total root exudate in the process of Tsr factor synthesis by R. leguminosarum. The fl ...
... root exudate (15). As naringenin, apigenin, and some other flavonoids can replace exudate for the induction of the nodABC promoter (21), we investigated whether naringenin or apigenin alone is sufficient to replace total root exudate in the process of Tsr factor synthesis by R. leguminosarum. The fl ...
File
... Mendel chose to work with edible pea, Pisum sativum, since seeds for numerous varieties were available from local seeds man. Mendel started with 34 varieties of peas and spent 2 years selecting those varieties that he would use in his experiments. He verified that each variety was genetically pure ( ...
... Mendel chose to work with edible pea, Pisum sativum, since seeds for numerous varieties were available from local seeds man. Mendel started with 34 varieties of peas and spent 2 years selecting those varieties that he would use in his experiments. He verified that each variety was genetically pure ( ...
Fulltext PDF - Indian Academy of Sciences
... preparation and hybridization were described by Bao et al. (2009). GISH signals were detected with fluoresceinconjugated anti-digoxigenin antibodies, and the slides were finally mounted within a thin layer of Vectrashield antifade ...
... preparation and hybridization were described by Bao et al. (2009). GISH signals were detected with fluoresceinconjugated anti-digoxigenin antibodies, and the slides were finally mounted within a thin layer of Vectrashield antifade ...