- Wiley Online Library
... [1,3,13]. In retrospect, the nitrate reductase reported above should be the respiratory one encoded by narGHJI since the enzyme was shown later to be induced under anaerobiosis and not under the control of the global nitrogen regulatory network that operates in B. subtilis and which governs the expr ...
... [1,3,13]. In retrospect, the nitrate reductase reported above should be the respiratory one encoded by narGHJI since the enzyme was shown later to be induced under anaerobiosis and not under the control of the global nitrogen regulatory network that operates in B. subtilis and which governs the expr ...
Molecular Characterization of a Powdery Mildew Resistance Gene
... Wheat powdery mildew, caused by the obligate biotrophic parasitic fungus Blumeria graminis (DC) E.O. Speer f. sp. tritici, is an important foliar disease of wheat worldwide and is promulgated by the heavy use of high-yielding semidwarf cultivars, irrigation, and nitrogen fertilizer (1). Resistance t ...
... Wheat powdery mildew, caused by the obligate biotrophic parasitic fungus Blumeria graminis (DC) E.O. Speer f. sp. tritici, is an important foliar disease of wheat worldwide and is promulgated by the heavy use of high-yielding semidwarf cultivars, irrigation, and nitrogen fertilizer (1). Resistance t ...
What`s in a Genotype? - CEUR Workshop Proceedings
... whose extent is only those bases that vary between the variant locus and the reference sequence specified in the reference genome. This „parts list‟ of a genotype outlined above represents one of many ways that a genome sequence can be partitioned into simpler elements. But it is important because i ...
... whose extent is only those bases that vary between the variant locus and the reference sequence specified in the reference genome. This „parts list‟ of a genotype outlined above represents one of many ways that a genome sequence can be partitioned into simpler elements. But it is important because i ...
Supplementary material for “Modularity in the genetic
... m11 and m10 imply that cancer phenotypes are not distributed randomly in the phenotype network. Dyadicity D 1 ( D 1 ) indicates that phenotypes in the disease class tend to connect more (less) densely among themselves than expected for a random configuration. Similarly heterophilicity H 1 ( H ...
... m11 and m10 imply that cancer phenotypes are not distributed randomly in the phenotype network. Dyadicity D 1 ( D 1 ) indicates that phenotypes in the disease class tend to connect more (less) densely among themselves than expected for a random configuration. Similarly heterophilicity H 1 ( H ...
SPT3 interacts with TFIID to allow normal transcription in
... confers several mutant phenotypes and causes alterations in transcription in vivo (Eisenmann et al. 1989). Here, we present further studies on sptl5-21 and report that the sptl 5-21 mutation changes a conserved residue of TFIID. This mutation does not alter the ability of TFIID to bind DNA or to int ...
... confers several mutant phenotypes and causes alterations in transcription in vivo (Eisenmann et al. 1989). Here, we present further studies on sptl5-21 and report that the sptl 5-21 mutation changes a conserved residue of TFIID. This mutation does not alter the ability of TFIID to bind DNA or to int ...
BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF THE
... RNA isolation, RT-PCR, sequencing. Total RNA (at final concentration of 1 μg/μl) was extracted from infected C. pepo var. giromontiina cv. ‘Astra Polka’ plants and purified with phenol-chloroform. RNA was dissolved in 40 l of sterile water and 1 l of solution was used for cDNA synthesis. First-str ...
... RNA isolation, RT-PCR, sequencing. Total RNA (at final concentration of 1 μg/μl) was extracted from infected C. pepo var. giromontiina cv. ‘Astra Polka’ plants and purified with phenol-chloroform. RNA was dissolved in 40 l of sterile water and 1 l of solution was used for cDNA synthesis. First-str ...
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... μl. The PCR products (including the three SNPs) were analyzed by direct sequencing using BigDye Terminator cycle sequencing on an ABI 3730XL POP7 DNA sequencing analysis 5.2 (Applied Biosystems). Statistical analysis The two groups were compared using Student unpaired t test. Hardy-Weinberg equilibr ...
... μl. The PCR products (including the three SNPs) were analyzed by direct sequencing using BigDye Terminator cycle sequencing on an ABI 3730XL POP7 DNA sequencing analysis 5.2 (Applied Biosystems). Statistical analysis The two groups were compared using Student unpaired t test. Hardy-Weinberg equilibr ...
RNA interference - Bio
... elicits a very limited unspecific response allowed the use of technology as a tool to assess gene function in mammalian cells. Because of its high efficiency and specificity, RNAi has revolutionized functional genomics and drug discovery. ...
... elicits a very limited unspecific response allowed the use of technology as a tool to assess gene function in mammalian cells. Because of its high efficiency and specificity, RNAi has revolutionized functional genomics and drug discovery. ...
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... accounting for about 95% of known cases (Patterson, 2003). The large Npc1 protein has 13 transmembrane domains and a sterolsensing domain (SSD) (Carstea et al., 1997; Loftus et al., 1997). Npc2, a small, secreted protein that binds cholesterol strongly, was first found as an abundant component of hu ...
... accounting for about 95% of known cases (Patterson, 2003). The large Npc1 protein has 13 transmembrane domains and a sterolsensing domain (SSD) (Carstea et al., 1997; Loftus et al., 1997). Npc2, a small, secreted protein that binds cholesterol strongly, was first found as an abundant component of hu ...
Online resources for genetic variation study-Part One
... Genotype: Each person has two copies of all chromosomes except the sex chromosomes. The set of alleles at a given locus forms the genotype. Genotyping: the process of identifying what genotype a person has for any given locus (loci). Whole-genome genotyping of all SNPs in a human genome? (11.8 mi ...
... Genotype: Each person has two copies of all chromosomes except the sex chromosomes. The set of alleles at a given locus forms the genotype. Genotyping: the process of identifying what genotype a person has for any given locus (loci). Whole-genome genotyping of all SNPs in a human genome? (11.8 mi ...
Inheritance and expression of transgenes in white clover
... present in a heterozygous form. Since white clover is outcrossing and predominantly self incompatible it is necessary to cross primary transgenic plants to produce an BC1 population, then intercross BC1 transgenic plants to produce an F2 population. The F2 population will have a highly heterogeneous ...
... present in a heterozygous form. Since white clover is outcrossing and predominantly self incompatible it is necessary to cross primary transgenic plants to produce an BC1 population, then intercross BC1 transgenic plants to produce an F2 population. The F2 population will have a highly heterogeneous ...
(FISH) or Immunohistochemistry (IHC)
... quantifying HER-2/neu gene amplification in formalin-fixed, paraffin-embedded breast cancer tissue specimens. These include but are not limited to the PathVysionTM HER-2 DNA Probe Kit (Vysis, Inc.) approved in 2001, the INFORM® her-2/neu test kit (Ventana Medical Systems, Inc.) approved in 2000, and ...
... quantifying HER-2/neu gene amplification in formalin-fixed, paraffin-embedded breast cancer tissue specimens. These include but are not limited to the PathVysionTM HER-2 DNA Probe Kit (Vysis, Inc.) approved in 2001, the INFORM® her-2/neu test kit (Ventana Medical Systems, Inc.) approved in 2000, and ...
File_details - Harvard PlasmID Database
... 3. Defining the Linker Sequences In the context of this analysis, “Linkers” refers to nucleotide sequences that flank the relevant CDS that will be evaluated on the nucleotide level but not at the amino acid level. From a molecular biology perspective, these are often thought of as “junction sequenc ...
... 3. Defining the Linker Sequences In the context of this analysis, “Linkers” refers to nucleotide sequences that flank the relevant CDS that will be evaluated on the nucleotide level but not at the amino acid level. From a molecular biology perspective, these are often thought of as “junction sequenc ...
Multiple RNA regulatory elements mediate distinct
... Pattern formation in the early development of many organisms relies on localized cytoplasmic proteins, which can be prelocalized as mRNAs. The Drosophila oskar gene, required both for posterior body patterning and germ cell determination, encodes one such mRNA. Localization of oskar mRNA is an elabo ...
... Pattern formation in the early development of many organisms relies on localized cytoplasmic proteins, which can be prelocalized as mRNAs. The Drosophila oskar gene, required both for posterior body patterning and germ cell determination, encodes one such mRNA. Localization of oskar mRNA is an elabo ...
Power Point Presentation
... color and wing size to find that body color and wing size are usually inherited together in specific combinations ...
... color and wing size to find that body color and wing size are usually inherited together in specific combinations ...
BMC Microbiology
... ppoD. Expression analysis confirmed that all three genes are indeed expressed under the conditions tested. Conclusion: A. niger produces the same oxylipins and has similar dioxygenase genes as A. nidulans. Their presence could point towards the existence of sexual reproduction in A. niger or a broad ...
... ppoD. Expression analysis confirmed that all three genes are indeed expressed under the conditions tested. Conclusion: A. niger produces the same oxylipins and has similar dioxygenase genes as A. nidulans. Their presence could point towards the existence of sexual reproduction in A. niger or a broad ...
A New Player in the Spermiogenesis Pathway of
... A suppressor screen of spe-27(it132ts) designed to identify additional members of the SPE-8 pathway turned up numerous mutations that suppress spe-27 mutant sterility (Muhlrad and Ward 2002). Interestingly, none of the spe-27 suppressors characterized so far are members of the SPE-8 group activation ...
... A suppressor screen of spe-27(it132ts) designed to identify additional members of the SPE-8 pathway turned up numerous mutations that suppress spe-27 mutant sterility (Muhlrad and Ward 2002). Interestingly, none of the spe-27 suppressors characterized so far are members of the SPE-8 group activation ...
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... some transposable elements can move between genomes (between individuals) as well as within an individual’s genome. Given their prevalence in genomes, the function (if any) of transposable elements has been much discussed but is little understood. It is not even clear whether transposable elements s ...
... some transposable elements can move between genomes (between individuals) as well as within an individual’s genome. Given their prevalence in genomes, the function (if any) of transposable elements has been much discussed but is little understood. It is not even clear whether transposable elements s ...
The Primary Structure of a 4.0-kDa Photosystem I Polypeptide
... Enzymatic cleavage of the PSI-I polypeptide with pepsin was Photosystem I (PS I)’ in plants and cyanobacteriacatalyzes the photochemical transfer of electrons from plastocyanin to carried out by dissolving the lyophilized PSI-I polypeptide (5 nmol) in 100 p l of 0.2% acetic acid (adjusted to pH 2.0 ...
... Enzymatic cleavage of the PSI-I polypeptide with pepsin was Photosystem I (PS I)’ in plants and cyanobacteriacatalyzes the photochemical transfer of electrons from plastocyanin to carried out by dissolving the lyophilized PSI-I polypeptide (5 nmol) in 100 p l of 0.2% acetic acid (adjusted to pH 2.0 ...
Williams syndrome deficits in visual spatial Chromosome 7q11.23
... Genomic DNA from Epstein-Barr virus transformed lymphoblastoid cell lines was prepared using Puregene DNA Isolation Kit (Gentra, Mineapolis, Minnesota). DNA samples isolated from subjects 1 to 3, from normal controls, and from WS subjects with typical deletions were then digested with PSTI, size fra ...
... Genomic DNA from Epstein-Barr virus transformed lymphoblastoid cell lines was prepared using Puregene DNA Isolation Kit (Gentra, Mineapolis, Minnesota). DNA samples isolated from subjects 1 to 3, from normal controls, and from WS subjects with typical deletions were then digested with PSTI, size fra ...
Association of Apolipoprotein E Alleles with Susceptibility to Age
... Apo E plays a pivotal role in lipid transportation and is known to be one of the drusen making elements (21). APOE is a polymorphic gene and makes a large number of different apo E isoforms of which the apoE2, apoE3 and apoE4, encoded by ε2, ε3 and ε4 allelic variants are the main isoforms identifie ...
... Apo E plays a pivotal role in lipid transportation and is known to be one of the drusen making elements (21). APOE is a polymorphic gene and makes a large number of different apo E isoforms of which the apoE2, apoE3 and apoE4, encoded by ε2, ε3 and ε4 allelic variants are the main isoforms identifie ...
Joint analysis of the influence of CYP11B1 and DGAT1 genetic
... DGAT1K) of son j within sire i, b1 and b2 are the regression coefficients representing half of the gene substitution effect (α/2), and eij is the random residual effect including polygenic and environmental effects. Sires were included as fixed effects because they represent highly selected animals ...
... DGAT1K) of son j within sire i, b1 and b2 are the regression coefficients representing half of the gene substitution effect (α/2), and eij is the random residual effect including polygenic and environmental effects. Sires were included as fixed effects because they represent highly selected animals ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.