Pre-made tetracycline regulator (TetR) expression lentiviral
... Lentivectors are HIV-1 (Human Immunodeficiency Virus 1) derived plasmids, used to generate lentiviral particles (lentivirus) that can be transduced into almost all kinds of mammalian cell types or organs, including stem cells, primary cells and non-dividing cells both in vivo and in cell culture sys ...
... Lentivectors are HIV-1 (Human Immunodeficiency Virus 1) derived plasmids, used to generate lentiviral particles (lentivirus) that can be transduced into almost all kinds of mammalian cell types or organs, including stem cells, primary cells and non-dividing cells both in vivo and in cell culture sys ...
Requirements for Human Medical Genome
... Medical testing by genomic methods shares many ethical, legal and social issues with other forms of clinical investigation. Existing issues of informed consent, incidental findings, the right not to know, family studies and re-contacting are potentially magnified due to the volume of information tha ...
... Medical testing by genomic methods shares many ethical, legal and social issues with other forms of clinical investigation. Existing issues of informed consent, incidental findings, the right not to know, family studies and re-contacting are potentially magnified due to the volume of information tha ...
Mutualism and asexual reproduction influence recognition genes in
... within the rab1, ITS, and Tef-1a regions examined. The recombination parameter R was calculated (Hudson et al. 1987), while the minimum number of recombination (RM) events during the history of the species (Hudson & Kaplan 1985) was estimated using neutral coalescence simulations, based on the numbe ...
... within the rab1, ITS, and Tef-1a regions examined. The recombination parameter R was calculated (Hudson et al. 1987), while the minimum number of recombination (RM) events during the history of the species (Hudson & Kaplan 1985) was estimated using neutral coalescence simulations, based on the numbe ...
Introduction To Genetics- Chapter 11
... 1. The different forms of a gene is called and an alleles. 2. The principal of dominance states that some alleles are dominant and others are recessive. ...
... 1. The different forms of a gene is called and an alleles. 2. The principal of dominance states that some alleles are dominant and others are recessive. ...
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... 1998; Iroskie et aI., 1999), AFLPs (Zabeau & Vos, 1993; Lottering et ai., 1999) and SIS markers (Gupta et ai., 1998; Naik et al., 1998; Seyfarth et al., 1999; Lottering et ...
... 1998; Iroskie et aI., 1999), AFLPs (Zabeau & Vos, 1993; Lottering et ai., 1999) and SIS markers (Gupta et ai., 1998; Naik et al., 1998; Seyfarth et al., 1999; Lottering et ...
the lysis/lysogeny switch in phage \lambda
... with no cooperativity does not lead to the same switch-like behaviour. ...
... with no cooperativity does not lead to the same switch-like behaviour. ...
Structure–function relationships of the variable - IMGT
... Technologies for the generation of full human rmAbs have been developed relatively recently and no fully human rmAb has been approved for cancer treatment to-date, however, several are currently in phase III trials.1 The five unconjugated therapeutic antibodies currently in use in oncology, are rmAb ...
... Technologies for the generation of full human rmAbs have been developed relatively recently and no fully human rmAb has been approved for cancer treatment to-date, however, several are currently in phase III trials.1 The five unconjugated therapeutic antibodies currently in use in oncology, are rmAb ...
L. LUZZATTO - per una vita come prima
... FROM SOMATIC MUTATIONS AND DARWINIAN SELECTION n-1 MUTATION ...
... FROM SOMATIC MUTATIONS AND DARWINIAN SELECTION n-1 MUTATION ...
出國報告電子檔規格
... aggressive and over than 75% of these tumors harbor TP53 mutations (14-18). DNA copy number alterations including chromosomal amplification, deletion and aneuploidy are the hallmarks of neoplasia (19). Amplification is one of the mechanisms that leads to an increase in activity of oncogenes and deve ...
... aggressive and over than 75% of these tumors harbor TP53 mutations (14-18). DNA copy number alterations including chromosomal amplification, deletion and aneuploidy are the hallmarks of neoplasia (19). Amplification is one of the mechanisms that leads to an increase in activity of oncogenes and deve ...
Leukaemia Section t(7;12)(q34;p13), t(12;14)(p13;q11) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... interphasic nuclei from a patient with t(7;12)(q34;p13) using CCND2 flanking probes at 12p13. The centromeric BAC clone RP11-388F6 (red) and the telomeric BAC clone RP11-320N7 (green) show one fusion signal at the normal chromosome 12, and dissociated signals at der(12) (red signal) and der(7) ...
... interphasic nuclei from a patient with t(7;12)(q34;p13) using CCND2 flanking probes at 12p13. The centromeric BAC clone RP11-388F6 (red) and the telomeric BAC clone RP11-320N7 (green) show one fusion signal at the normal chromosome 12, and dissociated signals at der(12) (red signal) and der(7) ...
Gene expression profiling of mice with genetically modified muscle
... ABI Prism 7700 Sequence Detection System [Applied Biosystems (ABI), Foster City, CA, U.S.A.] according to the manufacturer’s instructions. The following primers were designed using Primer ExpressTM 1.0 software: rGys1 (5 -tgtcacagatcaccgccatc-3 , 5 -ccaacggcctgaatgtga-3 ); mGys1 (5 -ccggctttggc ...
... ABI Prism 7700 Sequence Detection System [Applied Biosystems (ABI), Foster City, CA, U.S.A.] according to the manufacturer’s instructions. The following primers were designed using Primer ExpressTM 1.0 software: rGys1 (5 -tgtcacagatcaccgccatc-3 , 5 -ccaacggcctgaatgtga-3 ); mGys1 (5 -ccggctttggc ...
Characterization of Acetyl-CoA Carboxylases in the Basal
... over a diel light cycle. Previously, in A. carterae, global changes in mRNA abundance were observed over a diel light cycle. Previously, in A. carterae, global changes in mRNA abundance were observed near the light:dark transition [22,23]. The ACC transcripts closely followed this patter ...
... over a diel light cycle. Previously, in A. carterae, global changes in mRNA abundance were observed over a diel light cycle. Previously, in A. carterae, global changes in mRNA abundance were observed near the light:dark transition [22,23]. The ACC transcripts closely followed this patter ...
MARKER GENE TRANSFER TO WINTER DURUM WHEAT (Triticum
... 1100 psi and 6 cm distance displayed statistically significant differences (p<0.05). This also confirmed the higher values (number of blue spots) obtained from 650 psi-6 cm and 1100 psi-6 cm studies. In order to test the system by employing gold particles of 1.0 µm average diameter, four different p ...
... 1100 psi and 6 cm distance displayed statistically significant differences (p<0.05). This also confirmed the higher values (number of blue spots) obtained from 650 psi-6 cm and 1100 psi-6 cm studies. In order to test the system by employing gold particles of 1.0 µm average diameter, four different p ...
- eScholarship@UMMS - University of Massachusetts
... ChIP in the same HGPS and Father fibroblasts as in the H3K27me3 experiment (at passage 16) (Supplemental Table S1). Two different anti-lamin A/C antibodies, MAB3211 and N18, were used for two biological replicates. The correlation between replicates was high (R = 0.95). After filtering and normalizi ...
... ChIP in the same HGPS and Father fibroblasts as in the H3K27me3 experiment (at passage 16) (Supplemental Table S1). Two different anti-lamin A/C antibodies, MAB3211 and N18, were used for two biological replicates. The correlation between replicates was high (R = 0.95). After filtering and normalizi ...
Determination of primary structure
... There are no more than 50,000 protein-coding genes with ≤400 AA on average. This is ~20 x 106 possible unique sequences. So, a hexamer is not likely to appear more than once. Once you have at least 6 AA sequence, you can compare that to all possible proteins encoded in the entirety of the gene seque ...
... There are no more than 50,000 protein-coding genes with ≤400 AA on average. This is ~20 x 106 possible unique sequences. So, a hexamer is not likely to appear more than once. Once you have at least 6 AA sequence, you can compare that to all possible proteins encoded in the entirety of the gene seque ...
x-linked mental retardation
... proved to be particularly informative in the identification of genes that are involved in XLMR. Through the systematic characterization of patients with balanced X-chromosomal rearrangements, several additional genes were found to be truncated (see for example, REFS 11,12; FIG. 2). However, these ge ...
... proved to be particularly informative in the identification of genes that are involved in XLMR. Through the systematic characterization of patients with balanced X-chromosomal rearrangements, several additional genes were found to be truncated (see for example, REFS 11,12; FIG. 2). However, these ge ...
Comparative genomics provides evidence for the 3
... frames that show a high degree of sequence similarity to genes encoding enzymes in the C. aurantiacus pathway. Metagenomic DNA sequences from the microbial mats of alkaline siliceous hot springs also contain homologues of these genes that are highly similar to genes in both Roseiflexus spp. and Chlo ...
... frames that show a high degree of sequence similarity to genes encoding enzymes in the C. aurantiacus pathway. Metagenomic DNA sequences from the microbial mats of alkaline siliceous hot springs also contain homologues of these genes that are highly similar to genes in both Roseiflexus spp. and Chlo ...
A Fine Physical Map of Arabidopsis thaliana Chromosome 5
... YAC, PI, TAC and BAC libraries of the genome using the sequences of a variety of genetic and EST markers and terminal sequences of clones. The markers used were 88 genetic markers, 13 EST markers, 87 YAC end probes, 100 YAC subclone end probes, and 390 end probes of PI, TAC and BAC clones. The entir ...
... YAC, PI, TAC and BAC libraries of the genome using the sequences of a variety of genetic and EST markers and terminal sequences of clones. The markers used were 88 genetic markers, 13 EST markers, 87 YAC end probes, 100 YAC subclone end probes, and 390 end probes of PI, TAC and BAC clones. The entir ...
Role of tumor necrosis factor alpha gene polymorphism in childhood
... of the published asthma studies are from quite different population groups, which could account for some of their different findings, as the prevalence of polymerphisms within different racial groups is often markedly different21 .Comparisons of findings between different ethnic groups may be irrele ...
... of the published asthma studies are from quite different population groups, which could account for some of their different findings, as the prevalence of polymerphisms within different racial groups is often markedly different21 .Comparisons of findings between different ethnic groups may be irrele ...
Characteristics of a Laboratory Strain of Coleomegilla maculata with
... Insects were maintained as previously described [2] [3]. Individual insects were isolated for reciprocal straincrosses, and putative heterozygous first generation offspring (F1) crosses were pooled. Observed phenotype data were collected and entered into a spreadsheet, and phenotypic ratios were com ...
... Insects were maintained as previously described [2] [3]. Individual insects were isolated for reciprocal straincrosses, and putative heterozygous first generation offspring (F1) crosses were pooled. Observed phenotype data were collected and entered into a spreadsheet, and phenotypic ratios were com ...
Isolating, Cloning, and Sequencing DNA
... ends of each fragment (Figure 8-21). Ends of this type are known as cohesive ends, as each tail can form complementary base pairs with the tail at any other end produced by the same enzyme (Figure 8-22). The cohesive ends generated by restriction enzymes allow any two DNA fragments to be easily join ...
... ends of each fragment (Figure 8-21). Ends of this type are known as cohesive ends, as each tail can form complementary base pairs with the tail at any other end produced by the same enzyme (Figure 8-22). The cohesive ends generated by restriction enzymes allow any two DNA fragments to be easily join ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.