The tightly regulated promoter of the xanA gene of
... GATA factor AreA (Arst and Cove, 1973; Kudla et al., 1990). AreA is a, positive acting, transcription factor necessary for efficient transcription of most genes involved in the utilization of nitrogen sources (Arst and Cove, 1973; Kudla et al., 1990). Ammonium and glutamine repress transcription by ...
... GATA factor AreA (Arst and Cove, 1973; Kudla et al., 1990). AreA is a, positive acting, transcription factor necessary for efficient transcription of most genes involved in the utilization of nitrogen sources (Arst and Cove, 1973; Kudla et al., 1990). Ammonium and glutamine repress transcription by ...
Methods for detection of point mutations
... fragments [13]. With RNA, larger fragments can be analyzed [13]. Screening of multiple fragments can be achieved by either restriction digest of larger PCR fragments [14] or multiplex PCR [15, 16]. To identify potential mutations, SSCP has been combined with direct DNA sequencing [17]. In several ap ...
... fragments [13]. With RNA, larger fragments can be analyzed [13]. Screening of multiple fragments can be achieved by either restriction digest of larger PCR fragments [14] or multiplex PCR [15, 16]. To identify potential mutations, SSCP has been combined with direct DNA sequencing [17]. In several ap ...
mutations
... Mutations that involve changes in one or a few nucleotides are known as point mutations because they occur at a single point in the DNA sequence. They generally occur during replication. If a gene in one cell is altered, the alteration can be passed on to every cell that develops from the original o ...
... Mutations that involve changes in one or a few nucleotides are known as point mutations because they occur at a single point in the DNA sequence. They generally occur during replication. If a gene in one cell is altered, the alteration can be passed on to every cell that develops from the original o ...
NUCLEOTIDE and PROTEIN databases
... a wide range of web-based retrieval and analysis tools Is built primarily from the submission of sequence data from authors (i.e BankIt) and from bulk submission of high-throughput data from sequencing centre (i.e. Sequin) ...
... a wide range of web-based retrieval and analysis tools Is built primarily from the submission of sequence data from authors (i.e BankIt) and from bulk submission of high-throughput data from sequencing centre (i.e. Sequin) ...
Molecular Evolution of Overlapping Genes
... limitations, and compare the possible outcomes of estimating selection without ...
... limitations, and compare the possible outcomes of estimating selection without ...
Becker Muscular Dystrophy (BMD)
... creatine kinase. A muscle biopsy may be necessary to confirm that the diagnosis of BMD is correct. One the diagnosis is confirmed, genetic testing should be offered to identify the exact gene alteration in the dystrophin gene causing BMD on this occasion. If a gene alteration is identified, other fa ...
... creatine kinase. A muscle biopsy may be necessary to confirm that the diagnosis of BMD is correct. One the diagnosis is confirmed, genetic testing should be offered to identify the exact gene alteration in the dystrophin gene causing BMD on this occasion. If a gene alteration is identified, other fa ...
Interactions Between Genes Controlling Pathogenicity in the Flax
... segregating in these studies are different genes, it would seem, with the exception of five resistance genes that remain untested, that the pathogen possesses an avirulence gene specific and complementary to each of the resistance genes in the host. This one-for-one relationship, first elucidated by ...
... segregating in these studies are different genes, it would seem, with the exception of five resistance genes that remain untested, that the pathogen possesses an avirulence gene specific and complementary to each of the resistance genes in the host. This one-for-one relationship, first elucidated by ...
SensiFAST™ Two-Step Real-Time RT-PCR
... components during amplification and leading to greater sensitivity (Fig. 1). The addition of the latest advances in buffer chemistry and enhancers also ensures that the SensiFAST™ SYBR® kit produces faster (under 30 ...
... components during amplification and leading to greater sensitivity (Fig. 1). The addition of the latest advances in buffer chemistry and enhancers also ensures that the SensiFAST™ SYBR® kit produces faster (under 30 ...
Ref - SQA
... chromatids and formation of daughter nuclei. a ii Control of the cell cycle – notes changed to read “ Position and purpose of checkpoints... Mitosis promoting factor (MPF): protein complex controlling the entry of cells into mitosis. a ii Abnormal cell division: cancer cells. Notes amended to read “ ...
... chromatids and formation of daughter nuclei. a ii Control of the cell cycle – notes changed to read “ Position and purpose of checkpoints... Mitosis promoting factor (MPF): protein complex controlling the entry of cells into mitosis. a ii Abnormal cell division: cancer cells. Notes amended to read “ ...
Molecular Plant-Microbe Interactions 13:
... (1991). Gamma-aminobutyrate aminotransferase (GAAT, EC 1.6.1.19) activity was determined with a colorimetric reaction according to Yonaha and Toyama (1980). Glutamate decarboxylase (GAD, EC 4.1.1.15) activity was measured by the method of Fonda (1985) as modified by Vézina et al. (1988). The bacteri ...
... (1991). Gamma-aminobutyrate aminotransferase (GAAT, EC 1.6.1.19) activity was determined with a colorimetric reaction according to Yonaha and Toyama (1980). Glutamate decarboxylase (GAD, EC 4.1.1.15) activity was measured by the method of Fonda (1985) as modified by Vézina et al. (1988). The bacteri ...
High-order chromatin architecture determines the
... purifying selection alone; (2) comparisons with permuted data demonstrate the significant connection between megabase-level 3D chromatin structure and SCNA; (3) SCNA data reflect mutational mechanisms and purifying selection, in addition to commonly considered positive selection. These results argue ...
... purifying selection alone; (2) comparisons with permuted data demonstrate the significant connection between megabase-level 3D chromatin structure and SCNA; (3) SCNA data reflect mutational mechanisms and purifying selection, in addition to commonly considered positive selection. These results argue ...
Origin and evolution of Y chromosomes: Drosophila tales
... mentioning a caveat. Ideally, the complete gene set of the Y chromosomes in the 12 species should be available before starting a comparative analysis, similar to the approach used (at least approximately) for analysing the euchromatic portion of the other chromosomes. However, given the notorious di ...
... mentioning a caveat. Ideally, the complete gene set of the Y chromosomes in the 12 species should be available before starting a comparative analysis, similar to the approach used (at least approximately) for analysing the euchromatic portion of the other chromosomes. However, given the notorious di ...
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... varies within each district and among different districts of Basrah. This is in agreement with what was reported that βThalassemia mutations were very heterogeneous, and there are no specific distribution patterns that would aid in the identification of any ethnic background (Weatherall, 2000; Lahir ...
... varies within each district and among different districts of Basrah. This is in agreement with what was reported that βThalassemia mutations were very heterogeneous, and there are no specific distribution patterns that would aid in the identification of any ethnic background (Weatherall, 2000; Lahir ...
Punnett Squares
... Every individual offspring inherits at least two copies of every gene – one from the mother and one from the father. ...
... Every individual offspring inherits at least two copies of every gene – one from the mother and one from the father. ...
New functions of the Drosophila rhomboid gene
... Ruohola-Baker et al., 1993; J.W. O’Neill and E. Bier, unpublished data; M. Sturtevant and E. Bier, unpublished data – see Table 1 in this study), confirming the expectation that localized expression of rho is important for targeting EGF-R signaling to appropriate cells at a variety of developmental ...
... Ruohola-Baker et al., 1993; J.W. O’Neill and E. Bier, unpublished data; M. Sturtevant and E. Bier, unpublished data – see Table 1 in this study), confirming the expectation that localized expression of rho is important for targeting EGF-R signaling to appropriate cells at a variety of developmental ...
The linear chromosome of the plant
... number of ABC transporters for amino acids. In contrast, 'Ca. P. mali' has an extended set of genes for homologous recombination, excision repair and SOS response than 'Ca. P. asteris'. Conclusion: The small linear chromosome with large terminal inverted repeats and covalently closed hairpin ends, t ...
... number of ABC transporters for amino acids. In contrast, 'Ca. P. mali' has an extended set of genes for homologous recombination, excision repair and SOS response than 'Ca. P. asteris'. Conclusion: The small linear chromosome with large terminal inverted repeats and covalently closed hairpin ends, t ...
Punnett Squares
... Every individual offspring inherits at least two copies of every gene – one from the mother and one from the father. ...
... Every individual offspring inherits at least two copies of every gene – one from the mother and one from the father. ...
Pathway Analysis using Partek Genomics Suite® 6.6 and Partek
... genes within the pathway for each factor, respectively. A high score indicates that the genes which fall into the pathway have a low p-value for the given factor (i.e. display a greater degree of differential expression) ...
... genes within the pathway for each factor, respectively. A high score indicates that the genes which fall into the pathway have a low p-value for the given factor (i.e. display a greater degree of differential expression) ...
Myb genes enhance tobacco trichome production
... phenotype in Nicotiana tabacum. The data presented herein are in good agreement with their results which indicate that the transgene can also regulate trichome differentiation in tobacco. Suppression of the pleiotropic Arabidopsis ttg1-1 mutant by overexpression of the R gene from maize, a monocot, ...
... phenotype in Nicotiana tabacum. The data presented herein are in good agreement with their results which indicate that the transgene can also regulate trichome differentiation in tobacco. Suppression of the pleiotropic Arabidopsis ttg1-1 mutant by overexpression of the R gene from maize, a monocot, ...
Controlling Soil-borne cereal mosaic virus in the UK by developing
... Istabraq roots than in Claire roots after only 12 days, indicating the resistance mechanisms were activated at this early stage. Total RNA extractions were prepared from root samples taken 12 days after infection with SBCMV for cultivars Claire and Istabraq. The RDA method was carried out in both fo ...
... Istabraq roots than in Claire roots after only 12 days, indicating the resistance mechanisms were activated at this early stage. Total RNA extractions were prepared from root samples taken 12 days after infection with SBCMV for cultivars Claire and Istabraq. The RDA method was carried out in both fo ...
R4, a non-LTR retrotransposon specific to the
... The specific location of Rl and R2 elements within the 28S rRNA genes of the host simplifies their identification in other species by either Southern blotting or PCR amplification (1,18). PCR is the more sensitive of the two approaches and we are currently able to detect R1 and/or R2 elements in ins ...
... The specific location of Rl and R2 elements within the 28S rRNA genes of the host simplifies their identification in other species by either Southern blotting or PCR amplification (1,18). PCR is the more sensitive of the two approaches and we are currently able to detect R1 and/or R2 elements in ins ...
Characterization of the amino acid response element within the
... Total RNA was isolated from HepG2 cells using the Qiagen RNeasy kit (Qiagen) and including DNase I treatment before final elution to eliminate any DNA contamination. To measure the transcriptional activity from the SNAT2 gene, oligonucleotides derived from SNAT2 exon 4 and intron 4 were used to meas ...
... Total RNA was isolated from HepG2 cells using the Qiagen RNeasy kit (Qiagen) and including DNase I treatment before final elution to eliminate any DNA contamination. To measure the transcriptional activity from the SNAT2 gene, oligonucleotides derived from SNAT2 exon 4 and intron 4 were used to meas ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.